scholarly journals Comparison of a New 68Ga-Radiolabelled PET Imaging Agent sCD146 and RGD Peptide for In Vivo Evaluation of Angiogenesis in Mouse Model of Myocardial Infarction

Cells ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2305
Author(s):  
Anaïs Moyon ◽  
Philippe Garrigue ◽  
Samantha Fernandez ◽  
Fabien Hubert ◽  
Laure Balasse ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Functional Recovery ◽  
Pet Imaging ◽  
Vascular Diseases ◽  
Αvβ3 Integrin ◽  
Mice Model ◽  
In Vivo Evaluation ◽  
Tissue Fibrosis ◽  
18F Fdg

Ischemic vascular diseases are associated with elevated tissue expression of angiomotin (AMOT), a promising molecular target for PET imaging. On that basis, we developed an AMOT-targeting radiotracer, 68Ga-sCD146 and performed the first in vivo evaluation on a myocardial infarction mice model and then, compared AMOT expression and αvβ3-integrin expression with 68Ga-sCD146 and 68Ga-RGD2 imaging. After myocardial infarction (MI) induced by permanent ligation of the left anterior descending coronary artery, myocardial perfusion was evaluated by Doppler ultrasound and by 18F-FDG PET imaging. 68Ga-sCD146 and 68Ga-RGD2 PET imaging were performed. In myocardial infarction model, heart-to-muscle ratio of 68Ga-sCD146 imaging showed a significantly higher radiotracer uptake in the infarcted area of MI animals than in sham (* p = 0.04). Interestingly, we also observed significant correlations between 68Ga-sCD146 imaging and delayed residual perfusion assessed by 18F-FDG (* p = 0.04), with lowest tissue fibrosis assessed by histological staining (* p = 0.04) and with functional recovery assessed by ultrasound imaging (** p = 0.01). 68Ga-sCD146 demonstrated an increase in AMOT expression after MI. Altogether, significant correlations of early post-ischemic 68Ga-sCD146 uptake with late heart perfusion, lower tissue fibrosis and better functional recovery, make 68Ga-sCD146 a promising radiotracer for tissue angiogenesis assessment after MI.

scholarly journals Cardiac 18F-FDG Positron Emission Tomography: An Accurate Tool to Monitor In vivo Metabolic and Functional Alterations in Murine Myocardial Infarction

2021 ◽  
Vol 8 ◽  
Author(s):  
Maximilian Fischer ◽  
Mathias J. Zacherl ◽  
Ludwig Weckbach ◽  
Lisa Paintmayer ◽  
Tobias Weinberger ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Pet Imaging ◽  
Heart Function ◽  
Left Ventricular ◽  
Cardiac Monitoring ◽  
Cardiac Pet ◽  
Non Invasive ◽  
18F Fdg ◽  
Pet Scans

Cardiac monitoring after murine myocardial infarction, using serial non-invasive cardiac 18F-FDG positron emissions tomography (PET) represents a suitable and accurate tool for in vivo studies. Cardiac PET imaging enables tracking metabolic alterations, heart function parameters and provides correlations of the infarct size to histology. ECG-gated 18F-FDG PET scans using a dedicated small-animal PET scanner were performed in mice at baseline, 3, 14, and 30 days after myocardial infarct (MI) by permanent ligation of the left anterior descending (LAD) artery. The percentage of the injected dose per gram (%ID/g) in the heart, left ventricular metabolic volume (LVMV), myocardial defect, and left ventricular function parameters: end-diastolic volume (EDV), end-systolic volume (ESV), stroke volume (SV), and the ejection fraction (EF%) were estimated. PET assessment of the defect positively correlates with post-infarct histology at 3 and 30 days. Infarcted murine hearts show an immediate decrease in LVMV and an increase in %ID/g early after infarction, diminishing in the remodeling process. This study of serial cardiac PET scans provides insight for murine myocardial infarction models by novel infarct surrogate parameters. It depicts that serial PET imaging is a valid, accurate, and multimodal non-invasive assessment.

scholarly journals In Vivo Evaluation of11C-Preladenant for PET Imaging of Adenosine A2AReceptors in the Conscious Monkey

2017 ◽  
Vol 58 (5) ◽  
pp. 762-767 ◽  
Author(s):  
Xiaoyun Zhou ◽  
Ronald Boellaard ◽  
Kiichi Ishiwata ◽  
Muneyuki Sakata ◽  
Rudi A.J.O. Dierckx ◽  
...  
Keyword(s):  
Pet Imaging ◽  
In Vivo Evaluation

Synthesis and in vivo evaluation of [18F]N-(2-benzofuranylmethyl)-N′-[4-(2-fluoroethoxy)benzyl]piperazine, a novel σ1 receptor PET imaging agent

2011 ◽  
Vol 21 (22) ◽  
pp. 6820-6823 ◽  
Author(s):  
Iman A. Moussa ◽  
Samuel D. Banister ◽  
Nicolas Giboureau ◽  
Steven R. Meikle ◽  
Michael Kassiou
Keyword(s):  
Pet Imaging ◽  
Imaging Agent ◽  
In Vivo Evaluation ◽  
Σ1 Receptor

Improved radiosynthesis and preliminary in vivo evaluation of a 18F-labeled glycopeptide–peptoid hybrid for PET imaging of neurotensin receptor 2

2015 ◽  
Vol 23 (14) ◽  
pp. 4026-4033 ◽  
Author(s):  
Simone Maschauer ◽  
Cornelia Greff ◽  
Jürgen Einsiedel ◽  
Julian Ott ◽  
Philipp Tripal ◽  
...  
Keyword(s):  
Pet Imaging ◽  
In Vivo Evaluation ◽  
Neurotensin Receptor

68Ga-labeling and in vivo evaluation of a uPAR binding DOTA- and NODAGA-conjugated peptide for PET imaging of invasive cancers

2012 ◽  
Vol 39 (4) ◽  
pp. 560-569 ◽  
Author(s):  
Morten Persson ◽  
Jacob Madsen ◽  
Søren Østergaard ◽  
Michael Ploug ◽  
Andreas Kjaer
Keyword(s):  
Pet Imaging ◽  
In Vivo Evaluation

Radiosynthesis and in vivo evaluation of [ 11 C]MOV as a PET imaging agent for COX-2

2018 ◽  
Vol 28 (14) ◽  
pp. 2432-2435 ◽  
Author(s):  
Jaya Prabhakaran ◽  
Mark Underwood ◽  
Francesca Zanderigo ◽  
Norman R. Simpson ◽  
Anna R. Cooper ◽  
...  
Keyword(s):  
Pet Imaging ◽  
Imaging Agent ◽  
In Vivo Evaluation ◽  
Cox 2

scholarly journals In vitro and in vivo antileishmanial efficacy of a combination therapy of diminazene and artesunate against Leishmania donovani in BALB/c mice

2011 ◽  
Vol 53 (3) ◽  
pp. 129-132 ◽  
Author(s):  
Joshua Muli Mutiso ◽  
John Chege Macharia ◽  
Mustafa Barasa ◽  
Evans Taracha ◽  
Alain J. Bourdichon ◽  
...  
Keyword(s):  
Leishmania Donovani ◽  
Combined Therapy ◽  
Parasite Burden ◽  
Mice Model ◽  
In Vivo Evaluation ◽  
Reference Drug ◽  
Drug Treatments ◽  
Potential Use

The in vitro and in vivo activity of diminazene (Dim), artesunate (Art) and combination of Dim and Art (Dim-Art) against Leishmania donovani was compared to reference drug; amphotericin B. IC50 of Dim-Art was found to be 2.28 ± 0.24 µg/mL while those of Dim and Art were 9.16 ± 0.3 µg/mL and 4.64 ± 0.48 µg/mL respectively. The IC50 for Amphot B was 0.16 ± 0.32 µg/mL against stationary-phase promastigotes. In vivo evaluation in the L. donovani BALB/c mice model indicated that treatments with the combined drug therapy at doses of 12.5 mg/kg for 28 consecutive days significantly (p < 0.001) reduced parasite burden in the spleen as compared to the single drug treatments given at the same dosages. Although parasite burden was slightly lower (p < 0.05) in the Amphot B group than in the Dim-Art treatment group, the present study demonstrates the positive advantage and the potential use of the combined therapy of Dim-Art over the constituent drugs, Dim or Art when used alone. Further evaluation is recommended to determine the most efficacious combination ratio of the two compounds.

scholarly journals In vivo PET imaging with [18F]FDG to explain improved glucose uptake in an apolipoprotein A-I treated mouse model of diabetes

Diabetologia ◽  
2016 ◽  
Vol 59 (9) ◽  
pp. 1977-1984 ◽  
Author(s):  
Blake J. Cochran ◽  
William J. Ryder ◽  
Arvind Parmar ◽  
Shudi Tang ◽  
Anthonin Reilhac ◽  
...  
Keyword(s):  
Mouse Model ◽  
Glucose Uptake ◽  
Pet Imaging ◽  
Treated Mouse ◽  
Apolipoprotein A ◽  
18F Fdg
Sign in / Sign up

Export Citation Format

Share Document