scholarly journals Systemic Administration of PTH Supports Vascularization in Segmental Bone Defects Filled with Ceramic-Based Bone Graft Substitute

Cells ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 2058
Author(s):  
Holger Freischmidt ◽  
Jonas Armbruster ◽  
Emma Bonner ◽  
Thorsten Guehring ◽  
Dennis Nurjadi ◽  
...  
Keyword(s):  
Bone Graft ◽  
Donor Site ◽  
Bone Grafts ◽  
Bone Graft Substitute ◽  
Therapeutic Strategies ◽  
Care Providers ◽  
Sprague Dawley ◽  
Duration Of Treatment ◽  
Autologous Bone ◽  
Autologous Bone Grafts

Non-unions continue to present a challenge to trauma surgeons, as current treatment options are limited, duration of treatment is long, and the outcome often unsatisfactory. Additionally, standard treatment with autologous bone grafts is associated with comorbidity at the donor site. Therefore, alternatives to autologous bone grafts and further therapeutic strategies to improve on the outcome and reduce cost for care providers are desirable. In this study in Sprague–Dawley rats we employed a recently established sequential defect model, which provides a platform to test new potential therapeutic strategies on non-unions while gaining mechanistic insight into their actions. The effects of a combinatorial treatment of a bone graft substitute (HACaS+G) implantation and systemic PTH administration was assessed by µ-CT, histological analysis, and bio-mechanical testing and compared to monotreatment and controls. Although neither PTH alone nor the combination of a bone graft substitute and PTH led to the formation of a stable union, our data demonstrate a clear osteoinductive and osteoconductive effect of the bone graft substitute. Additionally, PTH administration was shown to induce vascularization, both as a single adjuvant treatment and in combination with the bone graft substitute. Thus, systemic PTH administration is a potential synergistic co-treatment to bone graft substitutes.

The Influence of Barrier Membranes on Autologous Bone Grafts

2008 ◽  
Vol 87 (11) ◽  
pp. 1048-1052 ◽  
Author(s):  
P.F.M. Gielkens ◽  
J. Schortinghuis ◽  
J.R. de Jong ◽  
A.M.J. Paans ◽  
J.L. Ruben ◽  
...  
Keyword(s):  
Bone Grafts ◽  
Sprague Dawley ◽  
Barrier Membranes ◽  
Barrier Membrane ◽  
Sprague Dawley Rats ◽  
Autologous Bone ◽  
Implant Dentistry ◽  
Autologous Bone Grafts ◽  
The Right ◽  
Microct Analysis

In implant dentistry, there is continuing debate regarding whether a barrier membrane should be applied to cover autologous bone grafts in jaw augmentation. A membrane would prevent graft remodeling with resorption and enhance graft incorporation. We hypothesized that membrane coverage does not effect resorption and incorporation of autologous onlay bone grafts. We treated 192 male Sprague-Dawley rats. A 4.0-mm-diameter bone graft was harvested from the right mandibular angle and transplanted to the left. Poly(DL-lactide-ε-caprolactone), collagen, and expanded polytetrafluoroethylene membranes were used to cover the grafts. The controls were left uncovered. Graft resorption at 2, 4, and 12 weeks was evaluated by post mortem microradiography and microCT. Analysis of the data showed no significant differences among the 4 groups. This demonstrates that the indication of barrier membrane use, to prevent bone remodeling with resorption and to enhance incorporation of autologous onlay bone grafts, is at least disputable.

scholarly journals The Use of Bone Grafts, Bone Graft Substitutes, and Orthobiologics for Osseous Healing in Foot and Ankle Surgery

2019 ◽  
Vol 4 (3) ◽  
pp. 247301141984901 ◽  
Author(s):  
Jonathan R. Peterson ◽  
Fangyu Chen ◽  
Eugene Nwankwo ◽  
Travis J. Dekker ◽  
Samuel B. Adams
Keyword(s):  
Bone Graft ◽  
Donor Site ◽  
Bone Grafts ◽  
Bone Graft Substitute ◽  
Autologous Bone Graft ◽  
Donor Site Morbidity ◽  
Foot And Ankle ◽  
Level Of Evidence ◽  
Ankle Surgery ◽  
Bone Graft Substitutes

Achieving fusion in osseous procedures about the foot and ankle presents unique challenges to the surgeon. Many patients have comorbidities that reduce osseous healing rates, and the limited space and high weightbearing demand placed on fusion sites makes the choice of bone graft, bone graft substitute, or orthobiologic agent of utmost importance. In this review, we discuss the essential characteristics of grafts, including their osteoconductive, osteoinductive, osteogenic, and angiogenic properties. Autologous bone graft remains the gold standard and contains all these properties. However, the convenience and lack of donor site morbidity of synthetic bone grafts, allografts, and orthobiologics, including growth factors and allogenic stem cells, has led to these being used commonly as augments. Level of Evidence: Level V, expert opinion.

Ethylene oxide gas sterilization: a simple technique for storing explanted skull bone

2007 ◽  
Vol 107 (2) ◽  
pp. 440-445 ◽  
Author(s):  
David H. Jho ◽  
Sergey Neckrysh ◽  
Julian Hardman ◽  
Fady T. Charbel ◽  
Sepideh Amin-Hanjani
Keyword(s):  
Ethylene Oxide ◽  
Decompressive Craniectomy ◽  
Room Temperature ◽  
Simple Technique ◽  
Functional Results ◽  
Bone Grafts ◽  
Increased Risk ◽  
Autologous Bone ◽  
Autologous Bone Grafts

✓ The authors evaluated the effectiveness of a simple technique using ethylene oxide (EtO) gas sterilization and room temperature storage of autologous bone grafts for reconstructive cranioplasty following decompressive craniectomy. The authors retrospectively analyzed data in 103 consecutive patients who underwent cranioplasty following decompressive craniectomy for any cause at the University of Illinois at Chicago between 1999 and 2005. Patients with a pre-existing intracranial infection prior to craniectomy or lost to follow-up before reconstruction were excluded. Autologous bone grafts were cleansed of soft tissue, hermetically sealed in sterilization pouches for EtO gas sterilization, and stored at room temperature until reconstructive cranioplasty was performed. Cranioplasties were performed an average of 4 months after decompressive craniectomy, and the follow-up after reconstruction averaged 14 months. Excellent aesthetic and functional results after single-stage reconstruction were achieved in 95 patients (92.2%) as confirmed on computed tomography. An infection of the bone flap occurred in eight patients (7.8%), and the skull defects were eventually reconstructed using polymethylmethacrylate with satisfactory results. The mean preservation interval was 3.8 months in patients with uninfected flaps and 6.4 months in those with infected flaps (p = 0.02). A preservation time beyond 10 months was associated with a significantly increased risk of flap infection postcranioplasty (odds ratio [OR] 10.8, p = 0.02). Additionally, patients who had undergone multiple craniotomies demonstrated a trend toward increased infection rates (OR 3.0, p = 0.13). Data in this analysis support the effectiveness of this method, which can be performed at any institution that provides EtO gas sterilization services. The findings also suggest that bone flaps preserved beyond 10 months using this technique should be discarded or resterilized prior to reconstruction.

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