scholarly journals The RNA-Binding Protein SBR (Dm NXF1) Is Required for the Constitution of Medulla Boundaries in Drosophila melanogaster Optic Lobes

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1144
Author(s):  
Ludmila Mamon ◽  
Anna Yakimova ◽  
Daria Kopytova ◽  
Elena Golubkova
Keyword(s):  
Drosophila Melanogaster ◽  
Glial Cells ◽  
Nuclear Export ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Cytoplasmic Localization ◽  
Optic Lobes ◽  
Axon Bundle

Drosophila melanogaster sbr (small bristles) is an orthologue of the Nxf1 (nuclear export factor 1) genes in different Opisthokonta. The known function of Nxf1 genes is the export of various mRNAs from the nucleus to the cytoplasm. The cytoplasmic localization of the SBR protein indicates that the nuclear export function is not the only function of this gene in Drosophila. RNA-binding protein SBR enriches the nucleus and cytoplasm of specific neurons and glial cells. In sbr12 mutant males, the disturbance of medulla boundaries correlates with the defects of photoreceptor axons pathfinding, axon bundle individualization, and developmental neurodegeneration. RNA-binding protein SBR participates in processes allowing axons to reach and identify their targets.

scholarly journals RNA-Binding Protein HuR Is Required for Stabilization of SLC11A1 mRNA and SLC11A1 Protein Expression

2005 ◽  
Vol 25 (18) ◽  
pp. 8139-8149 ◽  
Author(s):  
Yong Zhong Xu ◽  
Sergio Di Marco ◽  
Imed Gallouzi ◽  
Marek Rola-Pleszczynski ◽  
Danuta Radzioch
Keyword(s):  
Posttranscriptional Regulation ◽  
Macrophage Activation ◽  
Rna Binding ◽  
Binding Protein ◽  
Mrna Level ◽  
Rna Binding Protein ◽  
Cytoplasmic Localization ◽  
Differentiated Cells ◽  
Undifferentiated Cells ◽  
Solute Carrier

ABSTRACT The solute carrier family 11 member 1 (SLC11A1, formerly NRAMP1) gene is associated with infectious and autoimmune diseases and plays an important role in macrophage activation. Human SLC11A1 mRNA contains an AU-rich element (ARE) within the 3′ untranslated region; however, its role in the regulation of SLC11A1 gene expression has not been elucidated. Here we analyze the expression of SLC11A1 in human monocytes and HL-60 cells and then use HL-60 cells as a model to determine whether RNA-binding protein HuR is associated with the ARE and involved in SLC11A1 mRNA turnover. Our results demonstrate a binding of HuR to the SLC11A1 ARE in phorbol myristate acetate (PMA)-differentiated cells dramatically increased compared to that in undifferentiated cells. Interestingly, PMA-induced accumulation of cytoplasmic HuR occurs in parallel with an increase in the binding of HuR to SLC11A1 ARE and with an increase in the SLC11A1 mRNA level. This suggests that HuR's cytoplasmic localization plays an important role in the regulation of SLC11A1 expression. We also observe that down-regulation of HuR expression by RNA interference (RNAi) results in a decrease in SLC11A1 expression which can be restored by the addition of recombinant HuR protein to the RNAi-treated cells. Finally, we show that HuR overexpression in HL-60 cells significantly increases the SLC11A1 mRNA stability. Taken together, our data demonstrate that HuR is a key mediator of posttranscriptional regulation and expression of the SLC11A1 gene.

scholarly journals The RNA-binding protein FMRP facilitates the nuclear export of N6-methyladenosine–containing mRNAs

2019 ◽  
Vol 294 (52) ◽  
pp. 19889-19895 ◽  
Author(s):  
Phillip J. Hsu ◽  
Hailing Shi ◽  
Allen C. Zhu ◽  
Zhike Lu ◽  
Nimrod Miller ◽  
...  
Keyword(s):  
Nuclear Export ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein

scholarly journals The RNA-binding protein caper is required for sensory neuron development in Drosophila melanogaster

2017 ◽  
Vol 246 (8) ◽  
pp. 610-624 ◽  
Author(s):  
Eugenia C. Olesnicky ◽  
Jeremy M. Bono ◽  
Laura Bell ◽  
Logan T. Schachtner ◽  
Meghan C. Lybecker
Keyword(s):  
Drosophila Melanogaster ◽  
Sensory Neuron ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Neuron Development

scholarly journals The RNA-binding protein Staufen1 is increased in DM1 skeletal muscle and promotes alternative pre-mRNA splicing

2012 ◽  
Vol 196 (6) ◽  
pp. 699-712 ◽  
Author(s):  
Aymeric Ravel-Chapuis ◽  
Guy Bélanger ◽  
Ramesh S. Yadava ◽  
Mani S. Mahadevan ◽  
Luc DesGroseillers ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Alternative Splicing ◽  
Nuclear Export ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Splicing Regulation ◽  
Ribonucleic Acids ◽  
Dystrophia Myotonica Protein Kinase

In myotonic dystrophy type 1 (DM1), dystrophia myotonica protein kinase messenger ribonucleic acids (RNAs; mRNAs) with expanded CUG repeats (CUGexp) aggregate in the nucleus and become toxic to cells by sequestering and/or misregulating RNA-binding proteins, resulting in aberrant alternative splicing. In this paper, we find that the RNA-binding protein Staufen1 is markedly and specifically increased in skeletal muscle from DM1 mouse models and patients. We show that Staufen1 interacts with mutant CUGexp mRNAs and promotes their nuclear export and translation. This effect is critically dependent on the third double-stranded RNA–binding domain of Staufen1 and shuttling of Staufen1 into the nucleus via its nuclear localization signal. Moreover, we uncover a new role of Staufen1 in splicing regulation. Overexpression of Staufen1 rescues alternative splicing of two key pre-mRNAs known to be aberrantly spliced in DM1, suggesting its increased expression represents an adaptive response to the pathology. Altogether, our results unravel a novel function for Staufen1 in splicing regulation and indicate that it may positively modulate the complex DM1 phenotype, thereby revealing its potential as a therapeutic target.

scholarly journals The RNA-binding protein Sam68 is a multifunctional player in human cancer

2011 ◽  
Vol 18 (4) ◽  
pp. R91-R102 ◽  
Author(s):  
Pamela Bielli ◽  
Roberta Busà ◽  
Maria Paola Paronetto ◽  
Claudio Sette
Keyword(s):  
Nuclear Export ◽  
Rna Binding ◽  
Viral Infections ◽  
Human Cancer ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Mrna Translation ◽  
Endocrine Tumors ◽  
Recent Advancement ◽  
Kh Domain

Src associated in mitosis, of 68 kDa (Sam68) is a KH domain RNA-binding protein that belongs to the signal transduction and activation of RNA family. Although ubiquitously expressed, Sam68 plays very specialized roles in different cellular environments. In most cells, Sam68 resides in the nucleus and is involved in several steps of mRNA processing, from transcription, to alternative splicing, to nuclear export. In addition, Sam68 translocates to the cytoplasm upon cell stimulation, cell cycle transitions or viral infections, where it takes part to signaling complexes and associates with the mRNA translation machinery. Recent evidence has linked Sam68 function to the onset and progression of endocrine tumors, such as prostate and breast carcinomas. Notably, all the biochemical activities reported for Sam68 seem to be implicated in carcinogenesis. Herein, we review the recent advancement in the knowledge of Sam68 function and regulation and discuss it in the frame of its participation to neoplastic transformation and tumor progression.

scholarly journals The QKI-6 RNA Binding Protein Localizes with the MBP mRNAs in Stress Granules of Glial Cells

PLoS ONE ◽  
2010 ◽  
Vol 5 (9) ◽  
pp. e12824 ◽  
Author(s):  
Yunling Wang ◽  
Geneviève Lacroix ◽  
Jeffery Haines ◽  
Evgueni Doukhanine ◽  
Guillermina Almazan ◽  
...  
Keyword(s):  
Glial Cells ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Stress Granules
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