scholarly journals Metabolomic Variation Aligns with Two Geographically Distinct Subpopulations of Brachypodium Distachyon before and after Drought Stress

Cells ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 683
Author(s):  
Aleksandra Skalska ◽  
Manfred Beckmann ◽  
Fiona Corke ◽  
Gulsemin Savas Tuna ◽  
Metin Tuna ◽  
...  
Keyword(s):  
Drought Stress ◽  
High Resolution Mass Spectrometry ◽  
Brachypodium Distachyon ◽  
Principal Component ◽  
Population Level ◽  
Tca Cycle ◽  
Genomic Variation ◽  
Climatic Regions ◽  
Before And After ◽  
Model Grass

Brachypodium distachyon (Brachypodium) is a non-domesticated model grass that has been used to assess population level genomic variation. We have previously established a collection of 55 Brachypodium accessions that were sampled to reflect five different climatic regions of Turkey; designated 1a, 1c, 2, 3 and 4. Genomic and methylomic variation differentiated the collection into two subpopulations designated as coastal and central (respectively from regions 1a, 1c and the other from 2, 3 and 4) which were linked to environmental variables such as relative precipitation. Here, we assessed how far genomic variation would be reflected in the metabolomes and if this could be linked to an adaptive trait. Metabolites were extracted from eight-week-old seedlings from each accession and assessed using flow infusion high-resolution mass spectrometry (FIE-HRMS). Principal Component Analysis (PCA) of the derived metabolomes differentiated between samples from coastal and central subpopulations. The major sources of variation between seedling from the coastal and central subpopulations were identified. The central subpopulation was typified by significant increases in alanine, aspartate and glutamate metabolism and the tricarboxylic acid (TCA) cycle. Coastal subpopulation exhibited elevated levels of the auxin, indolacetic acid and rhamnose. The metabolomes of the seedling were also determined following the imposition of drought stress for seven days. The central subpopulation exhibited a metabolomic shift in response to drought, but no significant changes were seen in the coastal one. The drought responses in the central subpopulation were typified by changes in amino acids, increasing the glutamine that could be functioning as a stress signal. There were also changes in sugars that were likely to be an osmotic counter to drought, and changes in bioenergetic metabolism. These data indicate that genomic variation in our Turkish Brachypodium collection is largely reflected as distinctive metabolomes (“metabolotypes”) through which drought tolerance might be mediated.

scholarly journals Accelerated Growth Rate and Increased Drought Stress Resilience of the Model Grass Brachypodium distachyon Colonized by Bacillus subtilis B26

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0130456 ◽  
Author(s):  
François Gagné-Bourque ◽  
Boris F. Mayer ◽  
Jean-Benoit Charron ◽  
Hojatollah Vali ◽  
Annick Bertrand ◽  
...  
Keyword(s):  
Growth Rate ◽  
Bacillus Subtilis ◽  
Drought Stress ◽  
Brachypodium Distachyon ◽  
Stress Resilience ◽  
Accelerated Growth ◽  
Model Grass

scholarly journals Main Human Urinary Metabolites after Genipap (Genipa americana L.) Juice Intake

Nutrients ◽  
2018 ◽  
Vol 10 (9) ◽  
pp. 1155 ◽  
Author(s):  
Livia Dickson ◽  
Mathieu Tenon ◽  
Ljubica Svilar ◽  
Pascale Fança-Berthon ◽  
Raphael Lugan ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Metabolic Pathways ◽  
High Resolution Mass Spectrometry ◽  
Principal Component ◽  
Urinary Metabolites ◽  
Human Consumption ◽  
Healthy Male ◽  
Wide Range ◽  
Before And After ◽  
First Time

Genipap (Genipa americana L.) is a native fruit from Amazonia that contains bioactive compounds with a wide range of bioactivities. However, the response to genipap juice ingestion in the human exposome has never been studied. To identify biomarkers of genipap exposure, the untargeted metabolomics approach in human urine was applied. Urine samples from 16 healthy male volunteers, before and after drinking genipap juice, were analyzed by liquid chromatography–high-resolution mass spectrometry. XCMS package was used for data processing in the R environment and t-tests were applied on log-transformed and Pareto-scaled data to select the significant metabolites. The principal component analysis (PCA) score plots showed a clear distinction between experimental groups. Thirty-three metabolites were putatively annotated and the most discriminant were mainly related to the metabolic pathways of iridoids and phenolic derivatives. For the first time, the bioavailability of genipap iridoids after human consumption is reported. Dihydroxyhydrocinnamic acid, (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate, hydroxyhydrocinnamic acid, genipic acid, 12-demethylated-8-hydroxygenipinic acid, 3(7)-dehydrogenipinic acid, genipic acid glucuronide, nonate, and 3,4-dihydroxyphenylacetate may be considered biomarkers of genipap consumption. Human exposure to genipap reveals the production of derivative forms of bioactive compounds such as genipic and genipinic acid. These findings suggest that genipap consumption triggers effects on metabolic signatures.

scholarly journals Mid-Infrared Spectroscopy as a Rapid Tool to Qualitatively Predict the Effects of Species, Regions and Roasting on the Nutritional Composition of Australian Acacia Seed Species

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 1879
Author(s):  
Oladipupo Q. Adiamo ◽  
Yasmina Sultanbawa ◽  
Daniel Cozzolino
Keyword(s):  
Principal Component ◽  
Nutritional Composition ◽  
Human Consumption ◽  
Mid Infrared ◽  
Mir Spectroscopy ◽  
As Species ◽  
Before And After ◽  
Mir Spectra ◽  
Main Components ◽  
Rapid Tool

In recent times, the popularity of adding value to under-utilized legumes have increased to enhance their use for human consumption. Acacia seed (AS) is an underutilized legume with over 40 edible species found in Australia. The study aimed to qualitatively characterize the chemical composition of 14 common edible AS species from 27 regions in Australia using mid-infrared (MIR) spectroscopy as a rapid tool. Raw and roasted (180 °C, 5, 7, and 9 min) AS flour were analysed using MIR spectroscopy. The wavenumbers (1045 cm−1, 1641 cm−1, and 2852–2926 cm−1) in the MIR spectra show the main components in the AS samples. Principal component analysis (PCA) of the MIR data displayed the clustering of samples according to species and roasting treatment. However, regional differences within the same AS species have less of an effect on the components, as shown in the PCA plot. Statistical analysis of absorbance at specific wavenumbers showed that roasting significantly (p < 0.05) reduced the compositions of some of the AS species. The results provided a foundation for hypothesizing the compositional similarity and/or differences among AS species before and after roasting.

scholarly journals Examining the Effects of the Sacramento Dockless E-Bike Share on Bicycling and Driving

2021 ◽  
Vol 13 (1) ◽  
pp. 368
Author(s):  
Dillon T. Fitch ◽  
Hossain Mohiuddin ◽  
Susan L. Handy
Keyword(s):  
Travel Behavior ◽  
Regression Models ◽  
Population Level ◽  
Panel Survey ◽  
Multilevel Regression ◽  
Combine Data ◽  
Cross Sectional ◽  
Individual Level ◽  
Before And After ◽  
Bike Share

One way cities are looking to promote bicycling is by providing publicly or privately operated bike-share services, which enable individuals to rent bicycles for one-way trips. Although many studies have examined the use of bike-share services, little is known about how these services influence individual-level travel behavior more generally. In this study, we examine the behavior of users and non-users of a dockless, electric-assisted bike-share service in the Sacramento region of California. This service, operated by Jump until suspended due to the coronavirus pandemic, was one of the largest of its kind in the U.S., and spanned three California cities: Sacramento, West Sacramento, and Davis. We combine data from a repeat cross-sectional before-and-after survey of residents and a longitudinal panel survey of bike-share users with the goal of examining how the service influenced individual-level bicycling and driving. Results from multilevel regression models suggest that the effect of bike-share on average bicycling and driving at the population level is likely small. However, our results indicate that people who have used-bike share are likely to have increased their bicycling because of bike-share.

scholarly journals Grain dormancy and light quality effects on germination in the model grass Brachypodium distachyon

2011 ◽  
Vol 193 (2) ◽  
pp. 376-386 ◽  
Author(s):  
José M. Barrero ◽  
John V. Jacobsen ◽  
Mark J. Talbot ◽  
Rosemary G. White ◽  
Stephen M. Swain ◽  
...  
Keyword(s):  
Light Quality ◽  
Brachypodium Distachyon ◽  
Grain Dormancy ◽  
Model Grass

scholarly journals Influence of Stratospheric Sudden Warming on AIRS Midtropospheric CO2

2013 ◽  
Vol 70 (8) ◽  
pp. 2566-2573 ◽  
Author(s):  
Xun Jiang ◽  
Jingqian Wang ◽  
Edward T. Olsen ◽  
Thomas Pagano ◽  
Luke L. Chen ◽  
...  
Keyword(s):  
Large Scale ◽  
Principal Component ◽  
Department Of Energy ◽  
Atmospheric Infrared Sounder ◽  
Stratospheric Sudden Warming ◽  
Chemical Tracers ◽  
Before And After ◽  
Temporal And Spatial ◽  
Global Reanalysis ◽  
Height Data

Abstract Midtropospheric CO2 retrievals from the Atmospheric Infrared Sounder (AIRS) were used to explore the influence of stratospheric sudden warming (SSW) on CO2 in the middle to upper troposphere. To choose the SSW events that had strong coupling between the stratosphere and troposphere, the authors applied a principal component analysis to the NCEP/Department of Energy Global Reanalysis 2 (NCEP-2) geopotential height data at 17 pressure levels. Two events (April 2003 and March 2005) that have strong couplings between the stratosphere and troposphere were chosen to investigate the influence of SSW on AIRS midtropospheric CO2. The authors investigated the temporal and spatial variations of AIRS midtropospheric CO2 before and after the SSW events and found that the midtropospheric CO2 concentrations increased by 2–3 ppm within a few days after the SSW events. These results can be used to better understand how the chemical tracers respond to the large-scale dynamics in the high latitudes.

scholarly journals CHANGES IN FRUIT YIELD AND PHOTOSYNTHESIS PARAMETERS IN DIFFERENT OLIVE CULTIVARS (Olea europaea L.) UNDER CON-TRASTING WATER REGIMES

2020 ◽  
Vol 19 (3) ◽  
pp. 135-147
Author(s):  
Majid Golmohammadi ◽  
Omid Sofalian ◽  
Mehdi Taheri ◽  
Alireza Ghanbari ◽  
Valiollah Rasoli
Keyword(s):  
Drought Stress ◽  
Olea Europaea ◽  
Principal Component ◽  
Fruit Yield ◽  
Photosynthetic Parameters ◽  
Olive Cultivar ◽  
Drought Treatment ◽  
Olea Europaea L ◽  
Olive Cultivars ◽  
Olea Europaéa

The evergreen tree olive (Olea europaea L.) is the only species of the genus Olea that produces edible fruits with high ecological and economic value. This tree species has developed a series of physiochemical mechanisms to tolerate drought stress and grow under adverse climatic environments. One of these mechanisms is photosynthesis activities, so that as yet little information achieved about the relations between olive production and photosynthetic parameters under drought conditions. An experiment was carried out during two consecutive years (2015–2017) to study the response of 20 different olive tree cultivars (Olea europaea L.) to drought stress. Several parameters like net photosynthetic rate (PN), stomatal conductance (GS), transpiration rate (TE), photosynthetic pigments (total chlorophyll, chlorophyll a, b and carotenoid) and fruit yield were measured. The results of combined analysis of variance for fruit yield and other measured traits showed that year, drought treatment, cultivar main effects and their interactions were highly significant. The results indicated that drought stress reduced all traits, however GS (42.80%), PN (37.21%) and TE (37.17%) significantly affected by drought. Lower reduction in photosynthetic performance (PN, GS and TE) in the cultivar T7 compared to other olive cultivars allowed them to maintain better fruit yield. Principal component analysis (PCA) identified two PCs that accounted for 82.04 and 83.27% of the total variation in photosynthetic parameters under optimal and drought stress conditions, respectively. Taken together, mean comparison, relative changes due to drought and biplot analysis revealed that cultivars ‘T7’, ‘Roghani’, ‘Koroneiki’, ‘Korfolia’ and ‘Abou-satl’ displayed better response against drought stress. According to our results, one olive cultivar namely ‘T7’, could be used in olive breeding programs to improve new high yielding cultivars with drought tolerance for use in the drought-prone environments.

scholarly journals PROMOTING PROFESSIONAL IDENTITY AMONG PRE-SERVICE ENGLISH TEACHERS THROUGH TEACHER CAMPS

2021 ◽  
Vol 32 (2) ◽  
pp. 214
Author(s):  
Anderson Hidarto ◽  
Chindy Christine
Keyword(s):  
Professional Identity ◽  
Identity Formation ◽  
English Language ◽  
Principal Component ◽  
Language Teachers ◽  
Resource Exploitation ◽  
Before And After ◽  
Pre Service Teacher ◽  
Teaching Ideas

With a mission to enhance the quality of EFL teachers, the Regional English Language Office (RELO) of the U.S. Embassy in Indonesia has conducted a pre-service teacher camp where practical teaching innovations are taught alongside reflective practices and the development of social and intercultural competences. This paper aims to examine how the participants, who are pre-service language teachers, perceived their professional identity as potential EFL educators after attending Camp EPIC and to what extent the program contributed to this process of professional identity formation. The study employed a mixed-methods survey where a total of 45 participants of Camp EPIC 2018 were purposively selected. They were asked to provide descriptions of themselves as teachers before and after the teacher camp, which were then analysed qualitatively using thematic analysis. As for the quantitative data, a set of thirty-three Likert-scale items were examined using Principal Component Analysis (PCA). The triangulated data indicated that the participants’ perception was positively altered after the camp. While some mentioned that they were more resourceful and creative with teaching ideas, others showed a higher level of self-efficacy concerning their competence. These changes could be attributed to four major features of the camp: reflection, feedback, resource exploitation, and collaboration.

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