scholarly journals Panax ginseng-Derived Extracellular Vesicles Facilitate Anti-Senescence Effects in Human Skin Cells: An Eco-Friendly and Sustainable Way to Use Ginseng Substances

Cells ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 486
Author(s):  
Eun-Gyung Cho ◽  
Suh-Yeon Choi ◽  
Hyoseon Kim ◽  
Eun-Jeong Choi ◽  
Eun-Jeong Lee ◽  
...  
Keyword(s):  
Human Skin ◽  
Panax Ginseng ◽  
Extracellular Vesicles ◽  
Targeted Delivery ◽  
Biological Effects ◽  
Dermal Fibroblasts ◽  
Active Constituents ◽  
Skin Cells ◽  
Lipid Species ◽  
Human Skin Cells

Ginseng is a traditional herbal medicine in eastern Asian countries. Most active constituents in ginseng are prepared via fermentation or organic acid pretreatment. Extracellular vesicles (EVs) are released by most organisms from prokaryotes to eukaryotes and play central roles in intra- and inter-species communications. Plants produce EVs upon exposure to microbes; however, their direct functions and utility for human health are barely known, except for being proposed as delivery vehicles. In this study, we isolated EVs from ginseng roots (GrEVs) or the culture supernatants of ginseng cells (GcEVs) derived from Panax ginseng C.A. Meyer and investigated their biological effects on human skin cells. GrEV or GcEV treatments improved the replicative senescent or senescence-associated pigmented phenotypes of human dermal fibroblasts or ultraviolet B radiation-treated human melanocytes, respectively, by downregulating senescence-associated molecules and/or melanogenesis-related proteins. Based on comprehensive lipidomic analysis using liquid chromatography mass spectrometry, the lipidomic profile of GrEVs differed from that of the parental root extracts, showing significant increases in 70 of 188 identified lipid species and prominent increases in diacylglycerols, some phospholipids (phosphatidylcholine, phosphatidylethanolamine, lysophosphatidylcholine), and sphingomyelin, revealing their unique vesicular properties. Therefore, our results imply that GEVs represent a novel type of bioactive and sustainable nanomaterials that can be applied to human tissues for improving tissue conditions and targeted delivery of active constituents.

Biological effects of nitric oxide generated by an atmospheric pressure gas-plasma on human skin cells

Nitric Oxide ◽  
2011 ◽  
Vol 24 (1) ◽  
pp. 8-16 ◽  
Author(s):  
Joerg Liebmann ◽  
Joachim Scherer ◽  
Nikita Bibinov ◽  
Priyadarshini Rajasekaran ◽  
Reinhold Kovacs ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Human Skin ◽  
Atmospheric Pressure ◽  
Biological Effects ◽  
Skin Cells ◽  
Gas Plasma ◽  
Human Skin Cells

scholarly journals Can Light Emitted from Smartphone Screens and Taking Selfies Cause Premature Aging and Wrinkles?

2018 ◽  
Vol 8 (4Dec) ◽  
Author(s):  
N Arjmandi ◽  
Gh Mortazavi ◽  
S Zarei ◽  
M Faraz ◽  
S A R Mortazavi
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Visible Light ◽  
Human Skin ◽  
Blue Light ◽  
Biological Effects ◽  
Premature Aging ◽  
Oxygen Species ◽  
Skin Cells ◽  
Human Skin Cells

Since the early days of human life on the Earth, our skin has been exposed to different levels of light. Recently, due to inevitable consequences of modern life, humans are not exposed to adequate levels of natural light during the day but they are overexposed to relatively high levels of artificial light at night. Skin is a major target of oxidative stress and the link between aging and oxidative stress is well documented. Especially, extrinsic skin aging can be caused by oxidative stress. The widespread use of light emitting diodes (LEDs) and the rapidly increasing use of smartphones, tablets, laptops and desktop computers have led to a significant rise in the exposure of human eyes to short-wavelength visible light. Recent studies show that exposure of human skin cells to light emitted from electronic devices, even for exposures as short as 1 hour, may cause reactive oxygen species (ROS) generation, apoptosis, and necrosis. The biological effects of exposure to short-wavelength visible light in blue region in humans and other living organisms were among our research priorities at the Ionizing and Non-ionizing Radiation Protection Research Center (INIRPRC). Today, there is a growing concern over the safety of the light sources such as LEDs with peak emissions in the blue light range (400-490 nm). Recent studies aimed at investigating the effect of exposure to light emitted from electronic device on human skin cells, shows that even short exposures can increase the generation of reactive oxygen species. However, the biological effects of either long-term or repeated exposures are not fully known, yet. Furthermore, there are reports indicating that frequent exposure to visible light spectrum of the selfie flashes may cause skin damage and accelerated skin ageing. In this paper we have addressed the different aspects of potential effects of exposure to the light emitted from smartphones’ digital screens as well as smartphones’ photoflashes on premature aging of the human skin. Specifically, the effects of blue light on eyes and skin are discussed. Based on current knowledge, it can be suggested that changing the spectral output of LED-based smartphones’ flashes can be introduced as an effective method to reduce the adverse health effects associated with exposure to blue light.

scholarly journals Can Light Emitted from Smartphone Screens and Taking Selfies Cause Premature Aging and Wrinkles?

2018 ◽  
Vol 8 (4 Dec) ◽  
Author(s):  
N Arjmandi ◽  
Gh Mortazavi ◽  
S Zarei ◽  
M Faraz ◽  
S A R Mortazavi
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Visible Light ◽  
Human Skin ◽  
Blue Light ◽  
Biological Effects ◽  
Premature Aging ◽  
Oxygen Species ◽  
Skin Cells ◽  
Human Skin Cells

Since the early days of human life on the Earth, our skin has been exposed to different levels of light. Recently, due to inevitable consequences of modern life, humans are not exposed to adequate levels of natural light during the day but they are overexposed to relatively high levels of artificial light at night. Skin is a major target of oxidative stress and the link between aging and oxidative stress is well documented. Especially, extrinsic skin aging can be caused by oxidative stress. The widespread use of light emitting diodes (LEDs) and the rapidly increasing use of smartphones, tablets, laptops and desktop computers have led to a significant rise in the exposure of human eyes to short-wavelength visible light. Recent studies show that exposure of human skin cells to light emitted from electronic devices, even for exposures as short as 1 hour, may cause reactive oxygen species (ROS) generation, apoptosis, and necrosis. The biological effects of exposure to short-wavelength visible light in blue region in humans and other living organisms were among our research priorities at the Ionizing and Non-ionizing Radiation Protection Research Center (INIRPRC). Today, there is a growing concern over the safety of the light sources such as LEDs with peak emissions in the blue light range (400-490 nm). Recent studies aimed at investigating the effect of exposure to light emitted from electronic device on human skin cells, shows that even short exposures can increase the generation of reactive oxygen species. However, the biological effects of either long-term or repeated exposures are not fully known, yet. Furthermore, there are reports indicating that frequent exposure to visible light spectrum of the selfie flashes may cause skin damage and accelerated skin ageing. In this paper we have addressed the different aspects of potential effects of exposure to the light emitted from smartphones’ digital screens as well as smartphones’ photoflashes on premature aging of the human skin. Specifically, the effects of blue light on eyes and skin are discussed. Based on current knowledge, it can be suggested that changing the spectral output of LED-based smartphones’ flashes can be introduced as an effective method to reduce the adverse health effects associated with exposure to blue light.

Enhanced extracellular matrix synthesis using collagen dressings loaded with Artemisia absinthium plant extract

2018 ◽  
Vol 33 (5) ◽  
pp. 516-528 ◽  
Author(s):  
Alexandra Gaspar-Pintiliescu ◽  
Ana-Maria Seciu ◽  
Florin Miculescu ◽  
Lucia Moldovan ◽  
Elena Ganea ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Wound Healing ◽  
Human Skin ◽  
Composite Scaffold ◽  
Dermal Fibroblasts ◽  
Environment Interaction ◽  
High Porosity ◽  
Skin Cells ◽  
Extracellular Matrix Components ◽  
Human Skin Cells

The aim of this study was to develop three-dimensional porous composites of collagen (Col) incorporating polyphenolic-rich wormwood extract and to investigate their interaction with human skin cells, in order to optimize wound healing treatments. The scaffolds’ ultrastructure was observed by scanning electron microscopy, and biodegradability and bioactive compounds release were investigated in physiologic environment. Interaction of composites in direct and indirect contact with human skin cells was evaluated using two in vitro experimental models. ColWE scaffolds presented high porosity, swelling degree, and increased stability against enzymatic degradation, compared to Col scaffold. Composite scaffolds incorporating higher quantities of wormwood extract allowed better control of polyphenolics release. ColWE 0.5 variant favored the attachment and proliferation of human dermal fibroblasts and keratinocyte cells. In addition, the composite scaffold stimulated the synthesis of skin extracellular matrix components. All these results demonstrated that ColWE composites with improved physico-chemical and biological properties could be used in advanced wound healing applications.

scholarly journals Exploration of the Pressurization Condition for Killing Human Skin Cells and Skin Tumor Cells by High Hydrostatic Pressure

2020 ◽  
Vol 2020 ◽  
pp. 1-17 ◽  
Author(s):  
Toshihito Mitsui ◽  
Naoki Morimoto ◽  
Atsushi Mahara ◽  
Sharon Claudia Notodihardjo ◽  
Tien Minh Le ◽  
...  
Keyword(s):  
Hydrostatic Pressure ◽  
Tumor Cells ◽  
Human Skin ◽  
High Hydrostatic Pressure ◽  
Physical Method ◽  
Dermal Fibroblasts ◽  
Skin Tumor ◽  
Congenital Melanocytic Nevus ◽  
Skin Cells ◽  
Human Skin Cells

High hydrostatic pressure (HHP) is a physical method for inactivating cells or tissues without using chemicals such as detergents. We previously reported that HHP at 200 MPa for 10 min was able to inactivate all cells in skin and giant congenital melanocytic nevus (GCMN) without damaging the extracellular matrix. We also reported that HHP at 150 MPa for 10 min was not sufficient to inactivate them completely, while HHP at 200 MPa for 10 min was able to inactivate them completely. We intend to apply HHP to treat malignant skin tumor as the next step; however, the conditions necessary to kill each kind of cell have not been explored. In this work, we have performed a detailed experimental study on the critical pressure and pressurization time using five kinds of human skin cells and skin tumor cells, including keratinocytes (HEKas), dermal fibroblasts (HDFas), adipose tissue-derived stem cells (ASCs), epidermal melanocytes (HEMa-LPs), and malignant melanoma cells (MMs), using pressures between 150 and 200 MPa. We pressurized cells at 150, 160, 170, 180, or 190 MPa for 1 s, 2 min, and 10 min and evaluated the cellular activity using live/dead staining and proliferation assays. The proliferation assay revealed that HEKas were inactivated at a pressure higher than 150 MPa and a time period longer than 2 min, HDFas and MMs were inactivated at a pressure higher than 160 MPa and for 10 min, and ASCs and HEMa-LPs were inactivated at a pressure higher than 150 MPa and for 10 min. However, some HEMa-LPs were observed alive after HHP at 170 MPa for 10 min, so we concluded that HHP at a pressure higher than 180 MPa for 10 min was able to inactivate five kinds of cells completely.

scholarly journals Exogenous Antioxidants Impact on UV-Induced Changes in Membrane Phospholipids and the Effectiveness of the Endocannabinoid System in Human Skin Cells

Antioxidants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1260
Author(s):  
Agnieszka Gęgotek ◽  
Anna Jastrząb ◽  
Marta Dobrzyńska ◽  
Michał Biernacki ◽  
Elżbieta Skrzydlewska
Keyword(s):  
Ascorbic Acid ◽  
Cell Viability ◽  
Human Skin ◽  
Phospholipid Fatty Acid ◽  
Antioxidant Properties ◽  
Endocannabinoid System ◽  
Membrane Phospholipids ◽  
Skin Cells ◽  
Human Skin Cells ◽  
Induced Changes

Natural antioxidants effectively counteract changes caused by UV radiation in human skin cells. However, their action is limited due to their lipo/hydrophilicity. Therefore, the aim of this study was to analyze the mutual protective action of hydrophilic ascorbic acid and partially lipophilic rutin against UVA/UVB-induced changes in membranes phospholipid and endocannabinoid system in keratinocytes and fibroblasts. Obtained results clearly showed that, despite the stronger antioxidant properties of ascorbic acid, the lipid membranes were more effectively protected against UV-induced oxidation by rutin, including changes in phospholipid fatty acid levels, prevention against reactive aldehydes formation and endocannabinoids degradation. Ascorbic acid more strongly prevented UV-induced endocannabinoid receptors expression in fibroblasts, especially CB1. However, the combined action of used antioxidants resulted in the greatest cytoprotective effect, which was evident in the inflammatory marker TNFα down-regulation and increased cell viability following cell irradiation. The applied mixture of antioxidants showed a stronger protective in relation to membrane phospholipids in keratinocytes and in the endocannabinoid system in fibroblasts. In conclusion, it can be suggested that combined antioxidant capacities of ascorbic acid and rutin protects against lipid peroxidation but also decreases the UV-induced inflammation by direct interaction with the endocannabinoid system, thus increasing skin cell viability.

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