scholarly journals Chemically Defined Xeno- and Serum-Free Cell Culture Medium to Grow Human Adipose Stem Cells

Cells ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 466
Author(s):  
Stefano Panella ◽  
Francesco Muoio ◽  
Valentin Jossen ◽  
Yves Harder ◽  
Regine Eibl-Schindler ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Culture Medium ◽  
Ex Vivo ◽  
Free Cell ◽  
Adipose Stem Cells ◽  
Cell Culture Medium ◽  
Serum Free ◽  
A Cell ◽  
Human Adipose Stem Cells

Adipose tissue is an abundant source of stem cells. However, liposuction cannot yield cell quantities sufficient for direct applications in regenerative medicine. Therefore, the development of GMP-compliant ex vivo expansion protocols is required to ensure the production of a “cell drug” that is safe, reproducible, and cost-effective. Thus, we developed our own basal defined xeno- and serum-free cell culture medium (UrSuppe), specifically formulated to grow human adipose stem cells (hASCs). With this medium, we can directly culture the stromal vascular fraction (SVF) cells in defined cell culture conditions to obtain hASCs. Cells proliferate while remaining undifferentiated, as shown by Flow Cytometry (FACS), Quantitative Reverse Transcription PCR (RT-qPCR) assays, and their secretion products. Using the UrSuppe cell culture medium, maximum cell densities between 0.51 and 0.80 × 105 cells/cm2 (=2.55–4.00 × 105 cells/mL) were obtained. As the expansion of hASCs represents only the first step in a cell therapeutic protocol or further basic research studies, we formulated two chemically defined media to differentiate the expanded hASCs in white or beige/brown adipocytes. These new media could help translate research projects into the clinical application of hASCs and study ex vivo the biology in healthy and dysfunctional states of adipocytes and their precursors. Following the cell culture system developers’ practice and obvious reasons related to the formulas’ patentability, the defined media’s composition will not be disclosed in this study.

scholarly journals Development of a Biodegradable Microcarrier for the Cultivation of Human Adipose Stem Cells (hASCs) with a Defined Xeno- and Serum-Free Medium

2021 ◽  
Vol 11 (3) ◽  
pp. 925
Author(s):  
Francesco Muoio ◽  
Stefano Panella ◽  
Matias Lindner ◽  
Valentin Jossen ◽  
Yves Harder ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Culture Medium ◽  
Free Cell ◽  
Adipose Stem Cells ◽  
Cell Culture Medium ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Human Adipose Stem Cells

Stirred single-use bioreactors in combination with microcarriers (MCs) have established themselves as a technology that has the potential to meet the demands of current and future cell therapeutic markets. However, most of the published processes have been performed using fetal bovine serum (FBS) containing cell culture medium and non-biocompatible MCs. This approach has two significant drawbacks: firstly, the inevitable potential risks associated with the use of FBS for clinical applications; secondly, non-biocompatible MCs have to be removed from the cell suspension before implantation, requiring a step that causes loss of viable cells and adds further costs and complications. This study aimed to develop a new platform based on a chemically defined xeno- and serum-free cell culture medium and biodegradable MC that can support the growth of human adipose stem cells (hASCs) while still preserving their undifferentiated status. A specific combination of components and manufacturing parameters resulted in a MC prototype, called “BR44”, which delivered the desired functionality. MC BR44 allows the hASCs to stick to its surface and grow in a chemically defined xeno- and serum-free medium (UrSuppe). Although the cells’ expansion rate was not as high as with a commercial non-biodegradable standard MC, those cultured on BR44 maintained a better undifferentiated status in both static and dynamic conditions than those cultured on traditional 2D surfaces.

Development of a novel ELISA for human insulin using monoclonal antibodies produced in serum-free cell culture medium

2007 ◽  
Vol 40 (1-2) ◽  
pp. 98-103 ◽  
Author(s):  
Megha S. Even ◽  
Chad B. Sandusky ◽  
Neal D. Barnard ◽  
Jehangir Mistry ◽  
Madhur K. Sinha
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibodies ◽  
Human Insulin ◽  
Culture Medium ◽  
Free Cell ◽  
Cell Culture Medium ◽  
Serum Free

Preliminary clinical trials of serum free cell culture medium as a treatment for chronic atrophic ulcers

1997 ◽  
Vol 20 (3) ◽  
pp. 115-120 ◽  
Author(s):  
E. Lindenbaum ◽  
Y. Har-Shai ◽  
Y. Ullmann ◽  
A. L. Feitelberg ◽  
M. Tendler ◽  
...  
Keyword(s):  
Cell Culture ◽  
Clinical Trials ◽  
Culture Medium ◽  
Free Cell ◽  
Cell Culture Medium ◽  
Serum Free

Serum-free cell culture medium induces acceleration of wound healing in guinea-pigs

Burns ◽  
1995 ◽  
Vol 21 (2) ◽  
pp. 110-115 ◽  
Author(s):  
E.S. Lindenbaum ◽  
M. Tendler ◽  
D. Beach
Keyword(s):  
Cell Culture ◽  
Wound Healing ◽  
Guinea Pigs ◽  
Culture Medium ◽  
Free Cell ◽  
Cell Culture Medium ◽  
Serum Free

Production of Recombinant Proteins in Chinese Hamster Ovary Cells Using A Protein-Free Cell Culture Medium

1995 ◽  
Vol 13 (4) ◽  
pp. 389-392 ◽  
Author(s):  
Michael Zang ◽  
Helmut Trautmann ◽  
Christine Gandor ◽  
Ferruccio Messi ◽  
Fred Asselbergs ◽  
...  
Keyword(s):  
Cell Culture ◽  
Recombinant Proteins ◽  
Chinese Hamster Ovary ◽  
Culture Medium ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Free Cell ◽  
Cell Culture Medium ◽  
Ovary Cells

Low serum and serum-free culture of multipotential human adipose stem cells

Cytotherapy ◽  
2007 ◽  
Vol 9 (7) ◽  
pp. 637-646 ◽  
Author(s):  
A.M. Parker ◽  
H. Shang ◽  
M. Khurgel ◽  
A.J. Katz
Keyword(s):  
Stem Cells ◽  
Adipose Stem Cells ◽  
Serum Free ◽  
Human Adipose Stem Cells ◽  
Low Serum

scholarly journals Silk fibroin induces chondrogenic differentiation of canine adipose–derived multipotent mesenchymal stromal cells/mesenchymal stem cells

2019 ◽  
Vol 10 ◽  
pp. 204173141983505 ◽  
Author(s):  
Metka Voga ◽  
Natasa Drnovsek ◽  
Sasa Novak ◽  
Gregor Majdic
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Stromal Cells ◽  
Chondrogenic Differentiation ◽  
Culture Medium ◽  
Collagen Type ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Culture Conditions ◽  
Standard Cell ◽  
Cell Culture Medium

Under appropriate culture conditions, mesenchymal stem cells (MSC), also called more properly multipotent mesenchymal stromal cells (MMSC), can be induced toward differentiation into different cell lineages. In order to guide stem cell fate within an environment resembling the stem cell niche, different biomaterials are being developed. In the present study, we used silk fibroin (SF) as a biomaterial supporting the growth of MMSC and studied its effect on chondrogenesis of canine adipose–derived MMSC (cADMMSC). Adipose tissue was collected from nine privately owned dogs. MMSC were cultured on SF films and SF scaffolds in a standard cell culture medium. Cell morphology was evaluated by scanning electron microscopy (SEM). Chondrogenic differentiation was evaluated by alcian blue staining and mRNA expression of collagen type 1, collagen type 2, Sox9, and Aggrecan genes. cADMMSC cultured on SF films and SF scaffolds stained positive using alcian blue. SEM images revealed nodule-like structures with matrix vesicles and fibers resembling chondrogenic nodules. Gene expression of chondrogenic markers Sox9 and Aggrecan were statistically significantly upregulated in cADMMSC cultured on SF films in comparison to negative control cADMMSC. This result suggests that chondrogenesis of cADMMSC could occur when cells were grown on SF films in a standard cell culture medium without specific culture conditions, which were previously considered necessary for induction of chondrogenic differentiation.

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