scholarly journals Recent Advances in Biocatalysis and Metabolic Engineering

Catalysts ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1052
Author(s):  
Nien-Jen Hu ◽  
Si-Yu Li ◽  
Yung-Chuan Liu
Keyword(s):  
Metabolic Engineering ◽  
Chemical Reactions ◽  
Crude Cell Lysate ◽  
Intact Cells ◽  
Cell Lysate ◽  
Recent Advances ◽  
Synthetic Molecules

Biocatalysis refers to the utilization of enzymes, either in purified form, or existed as part of crude cell lysate or intact cells, to catalyze single- or multi-step chemical reactions, converting synthetic molecules or natural metabolites into high-value products [...]

Recent advances in lipid metabolic engineering of oleaginous yeasts

2021 ◽  
pp. 107722
Author(s):  
Atrayee Chattopadhyay ◽  
Mohor Mitra ◽  
Mrinal K. Maiti
Keyword(s):  
Metabolic Engineering ◽  
Oleaginous Yeasts ◽  
Recent Advances

Recent Advances in the Biosynthesis of Farnesene Using Metabolic Engineering

2021 ◽  
Author(s):  
Ruohao Tang ◽  
Qifeng Wen ◽  
Meijie Li ◽  
Wei Zhang ◽  
Zhaobao Wang ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Recent Advances

scholarly journals Engineered drug-loaded cells and cell derivatives as a delivery platform for cancer immunotherapy

2021 ◽  
Author(s):  
Lu Wang ◽  
Jinyao Liu
Keyword(s):  
Cancer Immunotherapy ◽  
Intact Cells ◽  
Recent Advances ◽  
Delivery Platform

Recent advances in improving cancer immunotherapy have been summarized with a focus on using functionalized intact cells and cell derivatives.

Recent advances of metabolic engineering strategies in natural isoprenoid production using cell factories

2020 ◽  
Vol 37 (1) ◽  
pp. 80-99 ◽  
Author(s):  
Meijie Li ◽  
Feifei Hou ◽  
Tong Wu ◽  
Xinglin Jiang ◽  
Fuli Li ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Microbial Production ◽  
Cell Factories ◽  
Recent Advances

This review covers the strategies mostly developed in the last three years for microbial production of isoprenoid, classified according to the engineering targets.

scholarly journals Intracellular calcium and calcineurin regulate neutrophil motility on vitronectin through a receptor identified by antibodies to integrins alphav and beta3

Blood ◽  
1996 ◽  
Vol 87 (5) ◽  
pp. 2038-2048 ◽  
Author(s):  
B Hendey ◽  
M Lawson ◽  
EE Marcantonio ◽  
FR Maxfield
Keyword(s):  
Flow Cytometry ◽  
Intracellular Calcium ◽  
Alpha Subunit ◽  
Intact Cells ◽  
Cell Lysate ◽  
Calcium Calmodulin Dependent ◽  
Quantitative Immunofluorescence ◽  
Peptide Maps ◽  
Neutrophil Cell ◽  
Rule Out

Abstract Buffering of intracellular calcium ([Ca2+]i) or inhibition of the calcium/calmodulin-dependent phosphatase, calcineurin, results in neutrophils being unable to detach from vitronectin with a consequent loss of motility. Treatment of [Ca2+]i-buffered or calcineurin- inhibited neutrophils with monoclonal antibodies (MoAbs) to beta3 or alphav beta3 integrins allowed neutrophils to detach and restored motility. Quantitative immunofluorescence and flow cytometry showed that MoAbs specific for beta3, alphav, or alphav beta3 integrins bind to neutrophils. Immunolocalization studies using antibodies to the highly conserved cytoplasmic domains of alphav and beta3 also identified the receptor on neutrophils. Whereas antibodies to alphav, alphav beta3, and beta3 recognized the receptor in intact cells, only the beta3 MoAb immunoprecipitated the receptor from a neutrophil cell lysate. The alpha subunit co-immunoprecipitated by the beta3 antibody reacted with an antibody to alphav by Western blot. Peptide maps of V8 protease digests showed a strong similarity in alpha and beta chains precipitated by antibodies to beta3 from neutrophils and endothelial cells. These results indicate that [Ca2+]i and calcineurin regulate neutrophil motility on vitronectin through an alphav beta3-like receptor. Although we cannot rule out the possibility that neutrophils have an isoform of alphav, such an isoform would have to be similar enough to react with alphav- and alphav beta3-specific MoAbs in intact cells.

Recent advances in the synthesis of oxaspirolactones and their application in the total synthesis of related natural products

2019 ◽  
Vol 17 (31) ◽  
pp. 7270-7292 ◽  
Author(s):  
Sagar S. Thorat ◽  
Ravindar Kontham
Keyword(s):  
Natural Products ◽  
Total Synthesis ◽  
Natural Product ◽  
Medicinal Chemistry ◽  
Natural Product Chemistry ◽  
Structural Motifs ◽  
Recent Advances ◽  
Synthetic Molecules ◽  
Product Chemistry

Oxaspirolactones are ubiquitous structural motifs found in natural products and synthetic molecules with a diverse biochemical and physicochemical profile, and represent a valuable target in natural product chemistry and medicinal chemistry.

Affinity induced immobilization of adenylate cyclase from the crude cell lysate for ATP conversion

2018 ◽  
Vol 164 ◽  
pp. 155-164 ◽  
Author(s):  
Qianqian Zhu ◽  
Wei Zhuang ◽  
Huanqing Niu ◽  
Lei Ge ◽  
Byron Villacorta Hernandez ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Crude Cell Lysate ◽  
Cell Lysate

Peptide Self-Assembled Monolayers for Label-Free and Unamplified Surface Plasmon Resonance Biosensing in Crude Cell Lysate

2009 ◽  
Vol 81 (16) ◽  
pp. 6779-6788 ◽  
Author(s):  
Olivier R. Bolduc ◽  
Christopher M. Clouthier ◽  
Joelle N. Pelletier ◽  
Jean-François Masson
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Self Assembled Monolayers ◽  
Label Free ◽  
Crude Cell Lysate ◽  
Cell Lysate ◽  
Assembled Monolayers ◽  
Self Assembled
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