scholarly journals PC 12 Pheochromocytoma Cell Response to Super High Frequency Terahertz Radiation from Synchrotron Source

Cancers ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 162 ◽  
Author(s):  
Palalle Perera ◽  
Dominique Appadoo ◽  
Samuel Cheeseman ◽  
Jason Wandiyanto ◽  
Denver Linklater ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Cell Membrane ◽  
Electromagnetic Radiation ◽  
High Frequency ◽  
Biological Effects ◽  
Cell Membrane Permeability ◽  
Temporary Increase ◽  
Thz Radiation ◽  
Synchrotron Source ◽  
Pc 12 Cells

High frequency (HF) electromagnetic fields (EMFs) have been widely used in many wireless communication devices, yet within the terahertz (THz) range, their effects on biological systems are poorly understood. In this study, electromagnetic radiation in the range of 0.3–19.5 × 1012 Hz, generated using a synchrotron light source, was used to investigate the response of PC 12 neuron-like pheochromocytoma cells to THz irradiation. The PC 12 cells remained viable and physiologically healthy, as confirmed by a panel of biological assays; however, exposure to THz radiation for 10 min at 25.2 ± 0.4 °C was sufficient to induce a temporary increase in their cell membrane permeability. High-resolution transmission electron microscopy (TEM) confirmed cell membrane permeabilization via visualisation of the translocation of silica nanospheres (d = 23.5 ± 0.2 nm) and their clusters (d = 63 nm) into the PC 12 cells. Analysis of scanning electron microscopy (SEM) micrographs revealed the formation of atypically large (up to 1 µm) blebs on the surface of PC 12 cells when exposed to THz radiation. Long-term analysis showed no substantial differences in metabolic activity between the PC 12 cells exposed to THz radiation and untreated cells; however, a higher population of the THz-treated PC 12 cells responded to the nerve growth factor (NGF) by extending longer neurites (up to 0–20 µm) compared to the untreated PC12 cells (up to 20 µm). These findings present implications for the development of nanoparticle-mediated drug delivery and gene therapy strategies since THz irradiation can promote nanoparticle uptake by cells without causing apoptosis, necrosis or physiological damage, as well as provide a deeper fundamental insight into the biological effects of environmental exposure of cells to electromagnetic radiation of super high frequencies.

The Analysis of the Influence of EMR Effects Provided From the Mobile Phones with Different SAR Index

2014 ◽  
Vol 555 ◽  
pp. 446-451
Author(s):  
Loredana Ungureanu ◽  
Luciana Cristea ◽  
Marina Bulmaga ◽  
Diana Cazangiu
Keyword(s):  
Electromagnetic Radiation ◽  
Mobile Phones ◽  
Human Tissue ◽  
High Frequency ◽  
Absorption Rate ◽  
Specific Absorption Rate ◽  
Human Factor ◽  
Biological Effects ◽  
Absorption Index ◽  
Electromagnetic Radiations

The researches realized on the biological effects of Electromagnetic Radiation (EMR) highlighted that the exposure of the human factor on the electromagnetic radiations of high frequency determines an excessive heating of the tissue causing different burning types. The aim of this paper is to analyze, by comparison, the effect of heating of the facial tissue under the Electromagnetic Radiation (EMR) influence provided from two mobile phones with different Specific Absorption Rate (SAR) index. Helping with the thermal maps, it determined the heating level of the human tissue exposed under the EMR generated by the phone during the conversation. The results obtained after the analysis are very useful for the evaluation of the heating level of the facial tissue in function of the SAR absorption index of the phone

scholarly journals Quantification of cell membrane permeability induced by monopolar and high-frequency bipolar bursts of electrical pulses

2016 ◽  
Vol 1858 (11) ◽  
pp. 2689-2698 ◽  
Author(s):  
Daniel C. Sweeney ◽  
Matej Reberšek ◽  
Janja Dermol ◽  
Lea Rems ◽  
Damijan Miklavčič ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
High Frequency ◽  
Cell Membrane Permeability ◽  
Electrical Pulses

scholarly journals Terahertz radiation may not alter rat basophilic leukemia cell membrane permeability to propidium iodide

Spectrum ◽  
2021 ◽  
Author(s):  
Savita Ayyalasomayajula ◽  
Steven Willows ◽  
Cameron Hough ◽  
Frank Hegmann ◽  
Marianna Kulka
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Terahertz Radiation ◽  
Propidium Iodide ◽  
Leukemia Cell ◽  
Cell Membrane Permeability ◽  
Leukemia Cell Line ◽  
Thz Radiation ◽  
Basophilic Leukemia Cell ◽  
Basophilic Leukemia

Terahertz radiation (THz) technology is fast-growing, with applications in sensing, security, monitoring and pharmaceutical industries. Since it is non-invasive, THz has been used as a diagnostic and therapeutic tool in medicine but its specific effects on biological systems is still largely under-studied. THz has been used to image tissues and cells mainly because it allows for identification of morphological features without the need for fluorescent or radioactive labels, but the potential effects of high intensities of THz radiation are currently not well understood.  One of the hypotheses that has been proposed for possible effects of THz on living cells, is that it disrupts the cell membrane and induces increased permeability. To test this hypothesis we exposed a rat basophilic leukemia cell line (RBL-2H3) to non-thermal intense THz radiation (duration, dose, etc) and observed the internalization of propidium iodide, a fluorescent intercalating agent that binds to DNA. We did not observe any changes in RBL-2H3 fluorescence following exposure to these intense THz pulses suggesting that exposure of RBL-2H3 to THz radiation may not increase their membrane permeability. These experiments were preliminary and further optimization and analysis is required before we can make definitive conclusions. However, our preliminary observations have set a baseline of RBL-2H3 internalization of propidium iodide, show that it is possible to expose RBL-2H3 cells to THz radiation using our configuration, and set the stage for future experiments.

scholarly journals Pinocembrin-7-Glucoside (P7G) Reduced Postharvest Blue Mold of Navel Orange by Suppressing Penicillium italicum Growth

2020 ◽  
Vol 8 (4) ◽  
pp. 536
Author(s):  
Chuying Chen ◽  
Jinyin Chen ◽  
Chunpeng Wan
Keyword(s):  
Electron Microscopy ◽  
Cell Membrane ◽  
Mycelial Growth ◽  
Cell Membrane Permeability ◽  
Wall Structure ◽  
Navel Orange ◽  
Penicillium Italicum ◽  
Blue Mold

The current study aimed to examine the in vitro and in vivo antifungal potential of pinocembrin-7-glucoside (P7G). P7G is an antifungal flavanone glycoside isolated from Ficus hirta Vahl. fruit against Penicillium italicum, a causative pathogen of blue mold disease in citrus fruit, and this study elucidates its possible action mechanism. P7G had a prominent mycelial growth inhibitory activity against P. italicum, with an observed half maximal effective concentration, minimum inhibitory concentration and minimum fungicidal concentration of 0.08, 0.2, and 0.8 g/L, respectively. The data from the in vivo test show that P7G significantly reduced blue mold symptoms and disease development of P. italicum in artificially inoculated “Newhall” navel orange. Compared to the control, increases in the cell membrane permeability of P. italicum supernatant and decreases in the intracellular constituent (e.g., soluble protein, reducing sugar, and total lipid) contents of P. italicum mycelia were identified, supporting scanning electron microscopy and transmission electron microscopy observations. Furthermore, a marked decline in both chitin and glucanase contents of P. italicum mycelia treated with P7G was induced by increasing its related degrading enzyme activities, suggesting that the cell wall structure was destroyed. The current study indicated that P7G may be a novel alternative for reducing blue mold by suppressing mycelial growth of P. italicum via a cell membrane/wall-targeting mechanism.

Sarcolemmal permeability changes during early myocardial anoxia

1978 ◽  
Vol 36 (2) ◽  
pp. 642-643
Author(s):  
M. Ashraf ◽  
L. Landa ◽  
L. Nimmo ◽  
C. M. Bloor
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Coronary Artery Occlusion ◽  
Artery Occlusion ◽  
Cell Membrane Permeability ◽  
Enzyme Release ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Sarcolemmal Permeability ◽  
Membrane Changes

Following coronary artery occlusion, the myocardial cells lose intracellular enzymes that appear in the serum 3 hrs later. By this time the cells in the ischemic zone have already undergone irreversible changes, and the cell membrane permeability is variably altered in the ischemic cells. At certain stages or intervals the cell membrane changes, allowing release of cytoplasmic enzymes. To correlate the changes in cell membrane permeability with the enzyme release, we used colloidal lanthanum (La+++) as a histological permeability marker in the isolated perfused hearts. The hearts removed from sprague-Dawley rats were perfused with standard Krebs-Henseleit medium gassed with 95% O2 + 5% CO2. The hypoxic medium contained mannitol instead of dextrose and was bubbled with 95% N2 + 5% CO2. The final osmolarity of the medium was 295 M osmol, pH 7. 4.

High-voltage electron microscopy of patch-clamped membranes

1987 ◽  
Vol 45 ◽  
pp. 582-583
Author(s):  
F. Sachs ◽  
M. J. Song
Keyword(s):  
Electron Microscopy ◽  
Cell Membrane ◽  
Patch Clamp ◽  
High Voltage Electron Microscopy ◽  
Small Patch ◽  
Cellular Electrophysiology ◽  
Voltage Electron ◽  
Electron Microscopes ◽  
Patch Technique ◽  
Membrane Patch

Cellular electrophysiology has been revolutionized by the introduction of patch clamp techniques. The patch clamp records current from a small patch of the cell membrane which has been sucked into a glass pipette. The membrane patch, a few micons in diameter, is attached to the glass by a seal which is electrically, diffusionally and mechanically tight. Because of the tight electrical seal, the noise level is low enough to record the activity of single ion channels over a time scale extending from 10μs to days. However, although the patch technique is over ten years old, the patch structure is unknown. The patch is inside a glass pipette where it has been impossible to see with standard electron microscopes. We show here that at 1 Mev the glass pipette is transparent and the membrane within can be seen with a resolution of about 30 A.

Numerical Modelling for Evaluation of Biological Effects Due to High Frequency Radiations in Indoor Environment

PIERS Online ◽  
2010 ◽  
Vol 6 (3) ◽  
pp. 247-251
Author(s):  
Matteo Cacciola ◽  
G. Megali ◽  
Diego Pellicano ◽  
M. Versaci ◽  
Francesco Carlo Morabito
Keyword(s):  
Numerical Modelling ◽  
High Frequency ◽  
Indoor Environment ◽  
Biological Effects
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