scholarly journals Atmospheric Pressure Plasma-Treated Carbon Nanowalls’ Surface-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (CNW-SALDI-MS)

2019 ◽  
Vol 5 (3) ◽  
pp. 40 ◽  
Author(s):  
Takayuki Ohta ◽  
Hironori Ito ◽  
Kenji Ishikawa ◽  
Hiroki Kondo ◽  
Mineo Hiramatsu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Atmospheric Pressure ◽  
Time Of Flight ◽  
Atmospheric Pressure Plasma ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Carbon Nanowalls

Carbon nanowalls (CNWs), vertically standing highly crystallizing graphene sheets, were used in the application of a surface-assisted laser desorption/ionization time-of-flight mass spectrometry (SALDI-TOF-MS). The CNW substrates solved the issues on interferences of matrix molecules and alkali metal addition ions in low-weight molecule detection. Before SALDI sample preparations, the hydrophobic CNW was treated by atmospheric pressure plasma for exposing hydrophilicity to the CNWs’ surface. Detection of water soluble amino acids, arginine, was demonstrated.

scholarly journals Characterization of Cryptosporidium parvum by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

2000 ◽  
Vol 66 (11) ◽  
pp. 4720-4724 ◽  
Author(s):  
Matthew L. Magnuson ◽  
James H. Owens ◽  
Catherine A. Kelty
Keyword(s):  
Mass Spectrometry ◽  
Cryptosporidium Parvum ◽  
Time Of Flight ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

ABSTRACT Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) was used to investigate whole and freeze-thawed Cryptosporidium parvum oocysts. Whole oocysts revealed some mass spectral features. Reproducible patterns of spectral markers and increased sensitivity were obtained after the oocysts were lysed with a freeze-thaw procedure. Spectral-marker patterns forC. parvum were distinguishable from those obtained forCryptosporidium muris. One spectral marker appears specific for the genus, while others appear specific at the species level. Three different C. parvum lots were investigated, and similar spectral markers were observed in each. Disinfection of the oocysts reduced and/or eliminated the patterns of spectral markers.

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