scholarly journals Motor Coordination Disorders Evaluated through the Grid Test and Changes in the Nigral Nrf2 mRNA Expression in Rats with Pedunculopontine Lesion

2020 ◽  
Vol 10 (10) ◽  
pp. 156
Author(s):  
Lisette Blanco-Lezcano ◽  
Esteban Alberti Amador ◽  
María Elena González Fraguela ◽  
Guadalupe Zaldívar Lelo de Larrea ◽  
Rosa Martha Pérez Serrano ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Motor Coordination ◽  
Pedunculopontine Nucleus ◽  
Control Groups ◽  
Post Injury ◽  
Coordination Disorders ◽  
Neurotoxic Lesion ◽  
Elevated Surface ◽  
The Right ◽  
Grid Test

Neurotoxic lesion of the pedunculopontine nucleus (PPN) is known to cause subtle motor dysfunctions. However, motor coordination during advance on a discontinuous and elevated surface has not been studied. It is also not known whether there are changes in the mRNA expression of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) in nigral tissue. Methods: The effects of the unilateral neurotoxic lesion of the PPN in motor coordination evaluated through grid test and Nrf2 mRNA expression in nigral tissue were evaluated. Two experimental designs (ED) were organized: ED#1 behavioral study (7 and 30 days after PPN lesion) and ED#2 molecular biology study (24 h, 48 h and 7 days) after PPN lesion. Results: ED#1—The number of faults made with left limbs, were significant higher in the lesioned groups (p < 0.01) both 7 and 30 days post-lesion. The number of failures made by the right limbs, was also significantly higher (p < 0.05) vs. control groups. ED#2—Nrf2 mRNA expression showed an increase 24 h after PPN injury (p < 0.01), followed by a peak of expression 48 h post injury (p < 0.001). Conclusions: Disorders of motor coordination associated with PPN injury are bilateral. The increased Nrf2 mRNA expression could represent an adaptive response to oxidative stress in the nigral tissue following pontine injury.

scholarly journals Nurr1, Pitx3, and α7 nAChRs mRNA Expression in Nigral Tissue of Rats with Pedunculopontine Neurotoxic Lesion

Medicina ◽  
2019 ◽  
Vol 55 (10) ◽  
pp. 616 ◽  
Author(s):  
Blanco-Lezcano ◽  
Alberti-Amador ◽  
González-Fraguela ◽  
Larrea ◽  
Pérez-Serrano ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mrna Expression ◽  
Cholinergic Neurons ◽  
Pedunculopontine Nucleus ◽  
Adult Brain ◽  
Related Factor ◽  
Nicotinic Cholinergic Receptors ◽  
Neurotoxic Lesion ◽  
Nigral Degeneration ◽  
Α7 Nachrs

Background and Objectives: The knowledge that the cholinergic neurons from pedunculopontine nucleus (PPN) are vulnerable to the degeneration in early stages of the Parkinson disease progression has opened new perspectives to the development of experimental model focused in pontine lesions that could increase the risk of nigral degeneration. In this context it is known that PPN lesioned rats exhibit early changes in the gene expression of proteins responsible for dopaminergic homeostasis. At the same time, it is known that nicotinic cholinergic receptors (nAChRs) mediate the excitatory influence of pontine-nigral projection. However, the effect of PPN injury on the expression of transcription factors that modulate dopaminergic neurotransmission in the adult brain as well as the α7 nAChRs gene expression has not been studied. The main objective of the present work was the study of the effects of the unilateral neurotoxic lesion of PPN in nuclear receptor-related factor 1 (Nurr1), paired-like homeodomain transcription factor 3 (Pitx3), and α7 nAChRs mRNA expression in nigral tissue. Materials and Methods: The molecular biology studies were performed by means of RT-PCR. The following experimental groups were organized: Non-treated rats, N-methyl-D-aspartate (NMDA)-lesioned rats, and Sham operated rats. Experimental subjects were sacrificed 24 h, 48 h and seven days after PPN lesion. Results: Nurr1 mRNA expression, showed a significant increase both 24 h (p < 0.001) and 48 h (p < 0.01) after PPN injury. Pitx3 mRNA expression evidenced a significant increase 24 h (p < 0.001) followed by a significant decrease 48 h and seven days after PPN lesion (p < 0.01). Finally, the α7 nAChRs nigral mRNA expression remained significantly diminished 24 h, 48 h (p < 0.001), and 7 days (p < 0.01) after PPN neurotoxic injury. Conclusion: Taking together these modifications could represent early warning signals and could be the preamble to nigral neurodegeneration events.

THEORETICAL SUBSTANTIATION OF IMPLEMENTATION OF KARATE TECHNIQUES IN CORRECTION AND DEVELOPMENT OF COORDINATION ABILITY COMPONENTS OF CHILDREN WITH HEARING IMPAIRMENTS

2020 ◽  
Vol 8 (1) ◽  
pp. 128-134
Author(s):  
Viktoriya Lerner ◽  
Galina Deryabina ◽  
Aleksey Filatkin ◽  
Yana Platonova
Keyword(s):  
Motor Coordination ◽  
Physical Culture ◽  
Hearing Impairments ◽  
Auditory Deprivation ◽  
Related Area ◽  
Auditory Analyzer ◽  
Motor Abilities ◽  
Theoretical Substantiation ◽  
The Right ◽  
Primary School Age

The aim. The karate competition requires the athlete to have a comprehensive manifestation of motor abilities. At the same time, the motor-related area is closely connected with function of the auditory analyzer. Consequently, auditory deprivation affects all types of motor abilities, but, first of all, it affects coordination abilities, since they are implemented on a defective basis of the sensory systems involved in controlling movements. Technical means (punches and kicks, blocks) and tactical means (instant assessment of the situation and making the right decisions) of traditional karate effectively affect the correction of various manifestations of motor coordination. Moreover, the above mentioned karate means affect motor coordination through motor experience in the form of complicating previously mastered movements and mastering new movements with increased coordination difficulties. Methods and organization of the study. We used the following set of complementary theoretical methods to meet the challenge of theoretical substantiation of the research: analysis of psychological, pedagogical and methodical literature, scientific articles on adaptive physical culture, abstracting, generalization and concretization. Results. The research revealed the most common deviations in the manifestation of various components of coordination abilities of children with auditory deprivation. We specified sensitive periods of development of this type of motor qualities in children with hearing impairments. We identified basic karate techniques and actions, which favorably influence the course of development of coordination abilities. Conclusion. Correction and development of the coordination abilities of children with hearing impairments represent the primary task of adaptive physical education. The most favorable age is primary school age. Techniques and actions of traditional karate have a high potential of effective impact on all types of coordination abilities of children.

Thrombosis and Altered expression of Intercellular Adhesion Molecule-1 (Icam-1) after Avulsion Injury in Rat Vessels

2004 ◽  
Vol 29 (3) ◽  
pp. 228-232 ◽  
Author(s):  
N. ISOGAI ◽  
H. TANAKA ◽  
S. ASAMURA
Keyword(s):  
Mrna Expression ◽  
Adhesion Molecule ◽  
Microscopic Analysis ◽  
Intercellular Adhesion ◽  
Intercellular Adhesion Molecule ◽  
Intercellular Adhesion Molecule 1 ◽  
Post Injury ◽  
Electron Microscopic ◽  
Altered Expression ◽  
Avulsion Injury

This study was undertaken to characterize the relative degrees of arterial and venous trauma after graded avulsion injuries. Rat femoral arteries and veins were subjected to reproducible avulsion injuries using forces of between 60 and 220 g. Thrombotic occlusion occurred at lower avulsion forces in veins than in arteries. Histologic and scanning electron microscopic analysis indicated increased endothelial disruption and exposed elastic lamina with increasing avulsion force in both vessels, but more prominently in arteries. Intercellular adhesion molecule-1 (ICAM-1) mRNA expression was evident at 3 and 6 hours after avulsion injury in veins, but only with higher avulsion force injuries in arteries. ICAM-1 mRNA expression was not found in either vessel before or after this 3 to 6 hour post-injury interval. These results indicate that the amount of avulsion force to which traumatized extremity vessels are subjected has a direct effect on the degree of intimal injury and subsequent thrombosis.

scholarly journals Comparative study of the areas of osteochondral defects produced in the femoral condyles of rabbits treated with gel of sugarcane biopolymer

2011 ◽  
Vol 26 (5) ◽  
pp. 383-386 ◽  
Author(s):  
Paulo Cezar Vidal Carneiro de Albuquerque ◽  
José Lamartine de Andrade Aguiar ◽  
Saulo Monteiro dos Santos ◽  
Nicodemus Pontes Filho ◽  
Roberto José Vieira de Mello ◽  
...  
Keyword(s):  
Sugar Cane ◽  
Digital Camera ◽  
Control Group ◽  
Study Group ◽  
Osteochondral Defects ◽  
Control Groups ◽  
The Right ◽  
And Control ◽  
Natural Healing ◽  
Femoral Condyles

PURPOSE: To measure the healed areas of osteochondral defects produced in femoral condyles of rabbits filled with biopolymer sugar cane gel and to compare these with those of the control group at 90, 120 and 180 days. METHODS: A study was made of 16 New Zealand rabbits, 6 and 7 months old, weighing between 2 and 2.5 kg. Defects of 3.2 x 4 mm were made, with trephine, in the femoral condyles of the right and left knees. As to the study group defects of the medial and lateral condyles of the right knee were used which were filled with Biopolymer Sugar Cane Gel; as to the Control Group defects of the medial and lateral condyles of the left t knees were used which were left open for natural healing. The defects were analyzed at 90, 120 and 180 days after surgery. After euthanasia, the knees were removed and fixed in Bouin's solution for later digital photographic documentation with a digital camera. The areas healed were measured in both the study and control groups using the images obtained from an Image-J® program. Statistical analysis was conducted using the non-parametric Mann-Whitney test. RESULTS: There were no significant differences between the means of the healed areas in the study and control groups at 90, 120 and 180 days after surgery. CONCLUSION: The dimension of the healed areas of the defects treated with the biopolymer sugar-cane gel in the study group was similar to those of the control group, which healed naturally.

Aging induces muscle-specific impairment of endothelium-dependent dilation in skeletal muscle feed arteries

2002 ◽  
Vol 93 (5) ◽  
pp. 1685-1690 ◽  
Author(s):  
Christopher R. Woodman ◽  
Elmer M. Price ◽  
M. Harold Laughlin
Keyword(s):  
Skeletal Muscle ◽  
Mrna Expression ◽  
Vascular Function ◽  
Fischer 344 ◽  
Old Rats ◽  
The Right ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide ◽  
Feed Arteries ◽  
Skeletal Muscle Feed Arteries

We tested the hypothesis that aging decreases endothelium-dependent vasodilation in feed arteries perfusing rat skeletal muscle. In addition, we tested the hypothesis that attenuated vasodilator responses are associated with decreased endothelial nitric oxide synthase (eNOS) and superoxide dismutase-1 (SOD-1) expression. Soleus feed arteries (SFA) and gastrocnemius feed arteries (GFA) were isolated from young (4 mo) and old (24 mo) male Fischer 344 rats. Feed arteries from the right hindlimb were cannulated with two glass micropipettes for examination of endothelium-dependent [acetylcholine (ACh)] and endothelium-independent [adenosine (Ado) or sodium nitroprusside (SNP)] vasodilator function. Feed arteries from the left hindlimb were frozen and used to assess eNOS and SOD-1 protein and mRNA expression. In SFA, endothelium-dependent dilation to ACh was reduced in old rats (0.9 ± 0.04 vs. 0.8 ± 0.03), whereas dilator responses to Ado and SNP were similar in SFA of young and old rats. In GFA, vasodilator responses to ACh, Ado, and SNP were not altered by age. eNOS and SOD-1 protein expression declined with age in SFA (−71 and −54%, respectively) but not in GFA. eNOS and SOD-1 mRNA expression were not altered by age in SFA or GFA. Collectively, these data indicate aging induces muscle-specific impairment of endothelium-dependent vascular function in SFA.

scholarly journals Evaluation of effect of local exogenous application of Myrrh oil on healing of wound incisions of facial skin (Histochemical, Histological and Histomorphometrical study in rabbits)

2019 ◽  
Vol 31 (4) ◽  
Author(s):  
Nawar Bahjet Kamil ◽  
Nada M. Al-Ghaban
Keyword(s):  
Healing Process ◽  
Inflammatory Cells ◽  
Control Group ◽  
Facial Skin ◽  
Histological Findings ◽  
Control Groups ◽  
Mean Values ◽  
Significant Difference ◽  
The Right ◽  
New Zealand Rabbits

Aim of the study: Is to evaluate the effect of myrrh oil local application on the healing process of skin wounds histologically , histomorphometrically and , histochemically. Materials and methods:Twenty male white New Zealand rabbits were used in this study. An incisional wounds with full thickness depth and of 2 cm length were done on both sides of the cheek skin of each rabbit. The left sided incisions (the control group) were irrigated with distilled water (10µL). The right sided incisions (the experimental groups) were treated with myrrh oil (10µL). Each group was subdivided into 4 subgroups according to the healing interval into 1,3,7 and 14 days(5 rabbits for each group). Results: Histological findings of our current study showed a highly significant difference between the experimental and the control groups in count of the inflammatory cells which showed that mean values increased with time for the control and the experimental groups. The histomorphometrical findings had shown that the thickness of the epithelium was nearly completed at about 7 days for the experimental groups and at about 14 days for the control. The blood vessels count was recorded to have a highly significant difference between the groups at days 1 and 3 only. The histochemical findings had shown that the collagen fibers remodeling had recorded a highly significant difference between the control and the experimental groups at days 7 and 14. Conclusion: The current study had revealed that myrrh oil accelerates wounds healing in rabbits. Key word:Wound healing , Myrrh oil, Masson’s Trichrome staining.

scholarly journals Clinical-enabled aspects after Neurotoxoplasmosis in a patient with immunosuppression: a case report

2021 ◽  
Author(s):  
Adrielle Galanti ◽  
Verônia Corrêa César Rodrigues ◽  
Daniela Maria Ribeiro Vaz ◽  
Ana Paula Oliveira Borges
Keyword(s):  
Case Report ◽  
Motor Coordination ◽  
Clinical Investigation ◽  
Functional Independence ◽  
Berg Balance Scale ◽  
Fine Motor ◽  
Cross Sectional ◽  
Right Cerebral Hemisphere ◽  
The Right ◽  
Immunodeficiency Syndrome

Background: Neurotoxoplasmosis is an opportunistic infection caused by the protozoan Toxoplasma Gondii, frequent in patients with Acquired Immunodeficiency Syndrome who become immunosuppressed by the presence of the disease. It can be presented by one or more brain abscesses, encephalitis or ventriculitis. Objectives: case report of a volunteer after neurological sequelae of neurotoxoplasmosis in order to highlight the relevant findings for a neurofunctional rehabilitation. Design and setting: This is an observational, cross-sectional case report type study conducted at the Clínica Escola de Fisioterapia of the University of Franca (UNIFRAN), with CEP approval (CAAE 83164918.2.0000.5495). Methods: Female patient, 53 years old. In the physiotherapy sector, neurological evaluation of the components was performed: sensitivity, movement, tone, reflexes, motor coordination, balance and gait. Balance and gait were assessed using the Berg Balance Scale, the Standing and Walking Test and the Dynamic Walking Index. Results: After clinical investigation, neurological toxoplasmosis infection was found in the right cerebral hemisphere. The physiotherapeutic evaluation showed the presence of left hemiparesis, the presence of spastic hypertonia, patellar and achilles hyperreflexia, the absence of fine motor skills and the presence of a reaping gait. The BSE result was 32 points, the TUG was 10.3 seconds and IMD was 20 points. Conclusion: The compromises found have an impact on the functionality of the volunteer. This study emphasizes the valorization of neurofunctional physiotherapeutic semiology for a treatment proposal that promotes greater functional independence.

scholarly journals Dust related respiratory pathology and pulmonary hypertension

2008 ◽  
pp. 77-80
Author(s):  
V. V. Razumov ◽  
N. A. Shatskikh ◽  
M. P. Zadorozhnaya
Keyword(s):  
Arterial Hypertension ◽  
Hemodynamic Parameters ◽  
Left Heart ◽  
Heart Ventricle ◽  
Control Groups ◽  
Right Heart ◽  
Pulmonary Hemodynamics ◽  
Initial Stage ◽  
Respiratory Pathology ◽  
The Right

Hemodynamic parameters of right and left heart ventricles and both greater and lesser blood circulations were studied using Doppler echocardiography in 129 patients with arterial hypertension, 54 patients with dust related respiratory pathology and 97 subjects of two control groups. Relying on the results we concluded that (1) the right heart ventricle and pulmonary circulation are targets for arterial hypertension, (2) pressure increase in arteries of lesser circulation occurs at the initial stage of dust related respiratory disease, (3) and the research of pulmonary hemodynamics with functional load should be obligatory when examining relationship between the disease and the profession.

The Effects of Bone Chips Dehydrated with Solvent on Healing Bone Defects

2002 ◽  
Vol 30 (2) ◽  
pp. 168-173 ◽  
Author(s):  
E Dayi ◽  
M Aslan ◽  
G Şimşek ◽  
AB Yilmaz
Keyword(s):  
Bone Marrow ◽  
Cross Sections ◽  
Bone Healing ◽  
Bone Grafts ◽  
Bone Defects ◽  
Control Groups ◽  
Bone Cortex ◽  
Healing Bone ◽  
The Right ◽  
And Control

The effect of bone chips dehydrated with solvent on the healing of bone defects was evaluated. Solvent-dehydrated spongiose bone chips were placed in experimentally formed cavities in the right back tibia of rabbits. After 10, 20 and 30 days, histopathological cross-sections from the bone grafts were examined microscopically for bone healing and formation of spongiose bone, cortex and bone marrow. Spongiose bone chips had a positive and accelerating influence on the healing of bone defects in the 10-day period after transplantation, but no significant differences were observed between the treated and control groups 20 and 30 days after transplantation.

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