scholarly journals High-Contrast Fluorescence Microscopy for a Biomolecular Analysis Based on Polarization Techniques Using an Optical Interference Mirror Slide

Biosensors ◽  
2014 ◽  
Vol 4 (4) ◽  
pp. 513-522 ◽  
Author(s):  
Mitsuru Yasuda ◽  
Takuo Akimoto
Keyword(s):  
Fluorescence Microscopy ◽  
High Contrast ◽  
Optical Interference ◽  
Interference Mirror ◽  
Biomolecular Analysis

Photolithographic Process Based on High Contrast Acrylate Photoresist for Multi-Protein Patterning

2006 ◽  
Vol 950 ◽  
Author(s):  
Margarita Chatzichristidi ◽  
Panagiota S. Petrou ◽  
Antonios M. Douvas ◽  
Constantinos D. Diakoumakos ◽  
Ioannis Raptis ◽  
...  
Keyword(s):  
Molecular Recognition ◽  
Fluorescence Microscopy ◽  
Protein Patterning ◽  
High Contrast ◽  
Sulfonium Salt ◽  
Photoacid Generator ◽  
Recognition Capability

ABSTRACTContinuing the effort of our group for biocompatible, photoresist-based lithographic processes, high contrast near UV photoresists are presented along with a novel multi-step lithographic scheme. The proposed lithographic scheme is based on successive lithographic steps (exposure at 310 nm) on the same substrate for the patterning of different proteins. The photoresists are based on (meth)acrylate tetrapolymers and a near UV sensitive sulfonium salt as photoacid generator and can be processed under mild baking and development conditions allowing patterning of proteins without denaturation and loss of their molecular recognition capability. Protein patterning in successive lithographic steps down to 2μm has been achieved. Sub-1μm patterning structures have been also obtained. Lithographic results were evaluated with SEM and fluorescence microscopy, after the recognition of the patterned biomolecules by their fluorescently labeled counterparts.

High-Contrast Imaging of Nano-Channels Using Reflection Near-Field Scanning Optical Microscope Enhanced by Optical Interference

2006 ◽  
Vol 13 (4) ◽  
pp. 266-268 ◽  
Author(s):  
Masaru Sakai ◽  
Shuji Mononobe ◽  
Shusaku Akiba ◽  
Akifumi Matsuda ◽  
Wakana Hara ◽  
...  
Keyword(s):  
Near Field ◽  
Optical Microscope ◽  
High Contrast ◽  
Contrast Imaging ◽  
Optical Interference ◽  
High Contrast Imaging ◽  
Near Field Scanning

Gold nanoparticle printed coverslips to facilitate fluorescence-TEM correlative microscopy

Microscopy ◽  
2017 ◽  
Vol 67 (1) ◽  
pp. 51-54
Author(s):  
Neeraj Prabhakar ◽  
Anni Määttänen ◽  
Jouko Peltonen ◽  
Pekka Hänninen ◽  
Markus Peurla ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Fluorescence Microscopy ◽  
Gold Nanoparticle ◽  
Cell Tracking ◽  
Light And Electron Microscopy ◽  
High Contrast ◽  
Correlative Microscopy ◽  
Glass Coverslip ◽  
Nano Gold ◽  
Commercial Glass

Abstract Correlative light and electron microscopy (CLEM) allows combining the advantages of fluorescence microscopy and electron microscopy for cell imaging. Rare phenomenon expressing cells can be studied by specifically tagged fluorophores with fluorescence microscopy. Subsequently, cells can be fixed and ultra-structural details can be studied with transmission electron microscopy (TEM) at a higher resolution. However, precise landmarks are necessary to track the same cell throughout the CLEM process. In this technical report, we present a high contrast inkjet-printed gold nanoparticle patterns over commercial glass coverslip to facilitate cell tracking with correlative microscopy. High contrast and strong reflection from nano gold pattern can be used as a fixed landmark for cell identification with fluorescence microscopy. Nano gold printed letters over coverslips are visible in resin blocks, which can be further used to identify the cell of interest for performing sectioning of embedded cell blocks for TEM.

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