scholarly journals Microfluidic-Based Amplification-Free Bacterial DNA Detection by Dielectrophoretic Concentration and Fluorescent Resonance Energy Transfer Assisted in Situ Hybridization (FRET-ISH)

Biosensors ◽  
2012 ◽  
Vol 2 (4) ◽  
pp. 405-416 ◽  
Author(s):  
Michelle M. Packard ◽  
Maxim Shusteff ◽  
Evangelyn C. Alocilja
Keyword(s):  
Energy Transfer ◽  
In Situ Hybridization ◽  
Dna Detection ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Bacterial Dna ◽  
Fluorescent Resonance Energy Transfer

Fluorescent resonance energy transfer -based biosensor for detecting conformational changes of Pin1

2018 ◽  
Vol 505 (2) ◽  
pp. 399-404 ◽  
Author(s):  
Masafumi Hidaka ◽  
Emiko Okabe ◽  
Kodai Hatakeyama ◽  
Heather Zook ◽  
Chiyoko Uchida ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Conformational Changes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Fluorescent Resonance Energy Transfer

scholarly journals TCR–pMHC bond conformation controls TCR ligand discrimination

2019 ◽  
Vol 17 (3) ◽  
pp. 203-217 ◽  
Author(s):  
Dibyendu K. Sasmal ◽  
Wei Feng ◽  
Sobhan Roy ◽  
Peter Leung ◽  
Yanran He ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Energy Transfer ◽  
Feedback Loop ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Intrinsic Property ◽  
Unanswered Question ◽  
Structural Differences ◽  
Self Peptides

Abstract A major unanswered question is how a TCR discriminates between foreign and self-peptides presented on the APC surface. Here, we used in situ fluorescence resonance energy transfer (FRET) to measure the distances of single TCR–pMHC bonds and the conformations of individual TCR–CD3ζ receptors at the membranes of live primary T cells. We found that a TCR discriminates between closely related peptides by forming single TCR–pMHC bonds with different conformations, and the most potent pMHC forms the shortest bond. The bond conformation is an intrinsic property that is independent of the binding affinity and kinetics, TCR microcluster formation, and CD4 binding. The bond conformation dictates the degree of CD3ζ dissociation from the inner leaflet of the plasma membrane via a positive calcium signaling feedback loop to precisely control the accessibility of CD3ζ ITAMs for phosphorylation. Our data revealed the mechanism by which a TCR deciphers the structural differences among peptides via the TCR–pMHC bond conformation.

In situ manipulation of fluorescence resonance energy transfer between quantum dots and monolayer graphene oxide by laser irradiation

Nanoscale ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 1236-1244 ◽  
Author(s):  
Wenjun He ◽  
Chengbing Qin ◽  
Zhixing Qiao ◽  
Yani Gong ◽  
Xiaorong Zhang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Graphene Oxide ◽  
Energy Transfer ◽  
Laser Irradiation ◽  
Fluorescence Resonance Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Monolayer Graphene ◽  
Fluorescence Resonance

Fluorescence resonance energy transfer between CdSeTe/ZnS quantum dots and monolayer graphene oxide is in situ manipulated by laser irradiation.

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