scholarly journals A Portable, Label-Free, Reproducible Quartz Crystal Microbalance Immunochip for the Detection of Zearalenone in Food Samples

Biosensors ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 53
Author(s):  
Shengmiao Liu ◽  
Xinyu Liu ◽  
Qianwen Pan ◽  
Zhihan Dai ◽  
Mingfei Pan ◽  
...  
Keyword(s):  
Frequency Response ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Low Cost ◽  
High Specificity ◽  
Food Samples ◽  
Soy Sauce ◽  
Label Free ◽  
Qualitative Detection ◽  
Application Frequency

This research reports a portable immunochip, based on quartz crystal microbalance (QCM) for label-free, low-cost qualitative detection of zearalenone (ZEN) in food samples. The experimental parameters in the functionalization and working process were evaluated in detail, in order to achieve a high accuracy and sensitivity. Under optimal conditions, the ZEN concentration at an inhibition ratio of 50% and 15% of the proposed QCM immunochip achieved 3.41 µg L−1 and 0.37 µg L−1, respectively. This portable QCM immunochip also exhibited high specificity, no obvious cross-reaction to five structural analogs of ZEN, and showed other mycotoxins. It could finish the whole qualitative measurement within 30 min, showed good stability during the processes of preparation (SD < 5%, n = 9), storage (frequency response >90%, in PBS at 4 °C for 15 days), and application (frequency response >90% after being reused 6 times). The developed QCM immunochip obtained accurate and repeatable recovery results in ZEN analysis in the chosen food samples (corn, wheat flour, soy sauce, and milk), which had a high correlation (R2 = 0.9844) with that achieved by the HPLC–MS/MS method. In short, this work developed a portable, stable, and reproducible QCM immunochip that could be used for rapid, low-cost, and sensitively measurement of ZEN content in real food samples.

scholarly journals Quantitative Assessment of Periodontal Bacteria Using a Cell-Based Immunoassay with Functionalized Quartz Crystal Microbalance

Chemosensors ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 159
Author(s):  
Satit Rodphukdeekul ◽  
Miyuki Tabata ◽  
Chindanai Ratanaporncharoen ◽  
Yasuo Takeuchi ◽  
Pakpum Somboon ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Quartz Crystal Microbalance ◽  
Quantitative Assessment ◽  
Quartz Crystal ◽  
Dynamic Range ◽  
Gold Surface ◽  
Enzyme Linked Immunosorbent Assay ◽  
Inflammatory Disorder ◽  
Label Free ◽  
Periodontal Bacteria

Periodontal disease is an inflammatory disorder that is triggered by bacterial plaque and causes the destruction of the tooth-supporting tissues leading to tooth loss. Several bacteria species, including Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans, are considered to be associated with severe periodontal conditions. In this study, we demonstrated a quartz crystal microbalance (QCM) immunoassay for quantitative assessment of the periodontal bacteria, A. actinomycetemcomitans. An immunosensor was constructed using a self-assembled monolayer of 11-mercaptoundecanoic acid (11-MUA) on the gold surface of a QCM chip. The 11-MUA layer was evaluated using a cyclic voltammetry technique to determine its mass and packing density. Next, a monoclonal antibody was covalently linked to 11-MUA using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide to act as the biorecognition element. The specificity of the monoclonal antibody was confirmed by an enzyme-linked immunosorbent assay. A calibration curve, for the relationship between the frequency shifts and number of bacteria, was used to calculate the number of A. actinomycetemcomitans bacteria in a test sample. Based on a regression equation, the lower detection limit was 800 cells, with a dynamic range up to 2.32 × 106 cells. Thus, the QCM biosensor in this study provides a sensitive and label-free method for quantitative analysis of periodontal bacteria. The method can be used in various biosensing assays for practical application and routine detection of periodontitis pathogens.

scholarly journals A Real-Time Method for Improving Stability of Monolithic Quartz Crystal Microbalance Operating under Harsh Environmental Conditions

Sensors ◽  
2021 ◽  
Vol 21 (12) ◽  
pp. 4166
Author(s):  
Román Fernández ◽  
María Calero ◽  
Yolanda Jiménez ◽  
Antonio Arnau
Keyword(s):  
Real Time ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Limit Of Detection ◽  
Quartz Substrate ◽  
Low Cost ◽  
Discrete Wavelet ◽  
Reagent Consumption ◽  
Measurement Cell ◽  
The Stability

Monolithic quartz crystal microbalance (MQCM) has recently emerged as a very promising technology suitable for biosensing applications. These devices consist of an array of miniaturized QCM sensors integrated within the same quartz substrate capable of detecting multiple target analytes simultaneously. Their relevant benefits include high throughput, low cost per sensor unit, low sample/reagent consumption and fast sensing response. Despite the great potential of MQCM, unwanted environmental factors (e.g., temperature, humidity, vibrations, or pressure) and perturbations intrinsic to the sensor setup (e.g., mechanical stress exerted by the measurement cell or electronic noise of the characterization system) can affect sensor stability, masking the signal of interest and degrading the limit of detection (LoD). Here, we present a method based on the discrete wavelet transform (DWT) to improve the stability of the resonance frequency and dissipation signals in real time. The method takes advantage of the similarity among the noise patterns of the resonators integrated in an MQCM device to mitigate disturbing factors that impact on sensor response. Performance of the method is validated by studying the adsorption of proteins (neutravidin and biotinylated albumin) under external controlled factors (temperature and pressure/flow rate) that simulate unwanted disturbances.

scholarly journals The Role of Wettability on the Response of a Quartz Crystal Microbalance Loaded with a Sessile Droplet

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Brandon Murray ◽  
Shankar Narayanan
Keyword(s):  
Contact Angle ◽  
Frequency Response ◽  
Quartz Crystal Microbalance ◽  
Contact Area ◽  
Quartz Crystal ◽  
Quartz Substrate ◽  
Dynamic Contact Angle ◽  
Dynamic Contact ◽  
Decay Length ◽  
Sessile Droplet

AbstractIn this work, the interaction between a sessile droplet’s contact angle and a quartz crystal microbalance (QCM) is elucidated. We differentiate the QCM’s frequency response to changes in the droplet contact area from variations in the dynamic contact angle. This is done by developing a computational model that couples the electrical and mechanical analysis of the quartz substrate with the visco-acoustic behavior of the sessile droplet. From our analysis, we conclude that changes in the contact angle have an effect on the frequency response of the QCM when the droplet height is on the order of the viscous decay length or smaller. On the other hand, changes in the interfacial contact area of the sessile droplets have a significant impact on the frequency response of the QCM regardless of the droplet size.

scholarly journals Rapid and Simultaneous Quantification of 4 Urinary Proteins by Piezoelectric Quartz Crystal Microbalance Immunosensor Array

2006 ◽  
Vol 52 (12) ◽  
pp. 2273-2280 ◽  
Author(s):  
Yang Luo ◽  
Ming Chen ◽  
Qianjun Wen ◽  
Meng Zhao ◽  
Bo Zhang ◽  
...  
Keyword(s):  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Clinical Chemistry ◽  
Label Free ◽  
Urinary Proteins ◽  
Quartz Crystals ◽  
Piezoelectric Quartz ◽  
Piezoelectric Immunosensor ◽  
Highly Sensitive ◽  
Piezoelectric Quartz Crystal

Abstract Background: Urinary proteins are predictive and prognostic markers for diabetes nephropathy. Conventional methods for the quantification of urinary proteins, however, are time-consuming, and most require radioactive labeling. We designed a label-free piezoelectric quartz crystal microbalance (QCM) immunosensor array to simultaneously quantify 4 urinary proteins. Methods: We constructed a 2 × 5 model piezoelectric immunosensor array fabricated with disposable quartz crystals for quantification of microalbumin, α1-microglobulin, β2-microglobulin, and IgG in urine. We made calibration curves after immobilization of antibodies at an optimal concentration and then evaluated the performance characteristics of the immunosensor with a series of tests. In addition, we measured 124 urine samples with both QCM immunosensor array and immunonephelometry to assess the correlation between the 2 methods. Results: With the QCM immunosensor array, we were able to quantify 4 urinary proteins within 15 min. This method had an analytical interval of 0.01–60 mg/L. The intraassay and interassay imprecisions (CVs) were &lt;10%, and the relative recovery rates were 90.3%–109.1%. Nonspecificity of the immunosensor was insignificant (frequency shifts &lt;20 Hz). ROC analyses indicated sensitivities were ≥95.8% and, specificities were ≥76.3%. Bland–Altman difference plots showed the immunosensor array to be highly comparable to immunonephelometry. Conclusions: The QCM system we designed has the advantages of being rapid, label free, and highly sensitive and thus can be a useful supplement to commercial assay methods in clinical chemistry.

Determination of Albumin in Urine by a Quartz Crystal Microbalance Label-Free Assay

2017 ◽  
Vol 50 (12) ◽  
pp. 1912-1925 ◽  
Author(s):  
Watcharinthon Theansun ◽  
Jittawat Sripratumporn ◽  
Chamras Promptmas
Keyword(s):  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Label Free

Diagnosis and genotyping of Plasmodium falciparum by a DNA biosensor based on quartz crystal microbalance (QCM)

2011 ◽  
Vol 49 (8) ◽  
Author(s):  
Tiparat Potipitak ◽  
Warunee Ngrenngarmlert ◽  
Chamras Promptmas ◽  
Sirinart Chomean ◽  
Wanida Ittarat
Keyword(s):  
Quartz Crystal Microbalance ◽  
Malaria Infection ◽  
Quartz Crystal ◽  
High Sensitivity ◽  
Cost Effective ◽  
Dna Biosensor ◽  
Dual Function ◽  
Label Free ◽  
Free Dna ◽  
Nanogram Level

AbstractMalaria infection withA label-free DNA biosensor based on quartz crystal microbalance (QCM) to diagnose and genotypeThe newly developed QCM was tested for its diagnosis ability using both malaria laboratory strains and clinical isolates. The biosensor was sensitive at the sub-nanogram level, specific for onlyThe dual function QCM was successfully developed with high sensitivity and specificity, and was cost-effective, stable and field adaptable.

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