scholarly journals Review of Integrated Optical Biosensors for Point-of-Care Applications

Biosensors ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 209
Author(s):  
Yung-Tsan Chen ◽  
Ya-Chu Lee ◽  
Yao-Hsuan Lai ◽  
Jin-Chun Lim ◽  
Nien-Tsu Huang ◽  
...  
Keyword(s):  
Optical Sensors ◽  
Point Of Care ◽  
Future Perspective ◽  
Optical Biosensors ◽  
Microfluidic Channels ◽  
Point Of Care Testing ◽  
Electrical Control ◽  
Semiconductor Chip ◽  
Integrated Optical ◽  
Signal Readout

This article reviews optical biosensors and their integration with microfluidic channels. The integrated biosensors have the advantages of higher accuracy and sensitivity because they can simultaneously monitor two or more parameters. They can further incorporate many functionalities such as electrical control and signal readout monolithically in a single semiconductor chip, making them ideal candidates for point-of-care testing. In this article, we discuss the applications by specifically looking into point-of-care testing (POCT) using integrated optical sensors. The requirement and future perspective of integrated optical biosensors for POC is addressed.

scholarly journals Nano-Interstice Driven Powerless Blood Plasma Extraction in a Membrane Filter Integrated Microfluidic Device

Sensors ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 1366
Author(s):  
Jaehoon Kim ◽  
Junghyo Yoon ◽  
Jae-Yeong Byun ◽  
Hyunho Kim ◽  
Sewoon Han ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Microfluidic Device ◽  
Blood Cells ◽  
Extraction Method ◽  
Membrane Filter ◽  
Point Of Care ◽  
Extraction Yield ◽  
Microfluidic Channels ◽  
Point Of Care Testing ◽  
Plasma Extraction

Blood plasma is a source of biomarkers in blood and a simple, fast, and easy extraction method is highly required for point-of-care testing (POCT) applications. This paper proposes a membrane filter integrated microfluidic device to extract blood plasma from whole blood, without any external instrumentation. A commercially available membrane filter was integrated with a newly designed dual-cover microfluidic device to avoid leakage of the extracted plasma and remaining blood cells. Nano-interstices installed on both sides of the microfluidic channels actively draw the extracted plasma from the membrane. The developed device successfully supplied 20 μL of extracted plasma with a high extraction yield (~45%) in 16 min.

scholarly journals High-accuracy quantitative principle of a new compact digital PCR equipment: Lab On An Array

2021 ◽  
Vol 19 (3) ◽  
pp. e34
Author(s):  
Haeun Lee ◽  
Cherl-Joon Lee ◽  
Dong Hee Kim ◽  
Chun-Sung Cho ◽  
Wonseok Shin ◽  
...  
Keyword(s):  
Target Gene ◽  
Point Of Care ◽  
Region Of Interest ◽  
Digital Pcr ◽  
Absolute Quantification ◽  
Micro Electro Mechanical System ◽  
High Accuracy ◽  
Point Of Care Testing ◽  
Semiconductor Chip ◽  
Compact Size

Digital PCR (dPCR) is the third-generation PCR that enables real-time absolute quantification without reference materials. Recently, global diagnosis companies have developed new dPCR equipment. In line with the development, the Lab On An Array (LOAA) dPCR analyzer (Optolane) was launched last year. The LOAA dPCR is a semiconductor chip-based separation PCR type equipment. The LOAA dPCR includes Micro Electro Mechanical System that can be injected by partitioning the target gene into 56 to 20,000 wells. The amount of target gene per wells is digitized to 0 or 1 as the number of well gradually increases to 20,000 wells because its principle follows Poisson distribution, which allows the LOAA dPCR to perform precise absolute quantification. LOAA determined region of interest first prior to dPCR operation. To exclude invalid wells for the quantification, the LOAA dPCR has applied various filtering methods using brightness, slope, baseline, and noise filters. As the coronavirus disease 2019 has now spread around the world, needs for diagnostic equipment of point of care testing (POCT) are increasing. The LOAA dPCR is expected to be suitable for POCT diagnosis due to its compact size and high accuracy. Here, we describe the quantitative principle of the LOAA dPCR and suggest that it can be applied to various fields.

scholarly journals A Compact, Syringe-Assisted, Vacuum-Driven Micropumping Device

Micromachines ◽  
2019 ◽  
Vol 10 (8) ◽  
pp. 543 ◽  
Author(s):  
Anyang Wang ◽  
Domin Koh ◽  
Philip Schneider ◽  
Evan Breloff ◽  
Kwang W. Oh
Keyword(s):  
Microfluidic Device ◽  
Point Of Care ◽  
Microfluidic Devices ◽  
Microfluidic Channels ◽  
Constant Flow ◽  
Proof Of Concept ◽  
Point Of Care Testing ◽  
Microfluidic Mixing ◽  
Pumping System ◽  
Dead End

In this paper, a simple syringe‑assisted pumping method is introduced. The proposed fluidic micropumping system can be used instead of a conventional pumping system which tends to be large, bulky, and expensive. The micropump was designed separately from the microfluidic channels and directly bonded to the outlet of the microfluidic device. The pump components were composed of a dead‑end channel which was surrounded by a microchamber. A syringe was then connected to the pump structure by a short tube, and the syringe plunger was manually pulled out to generate low pressure inside the microchamber. Once the sample was loaded in the inlet, air inside the channel diffused into the microchamber through the PDMS (polydimethylsiloxane) wall, acting as a dragging force and pulling the sample toward the outlet. A constant flow with a rate that ranged from 0.8 nl · s − 1 to 7.5 nl · s − 1 was achieved as a function of the geometry of the pump, i.e., the PDMS wall thickness and the diffusion area. As a proof-of-concept, microfluidic mixing was demonstrated without backflow. This method enables pumping for point-of-care testing (POCT) with greater flexibility in hand-held PDMS microfluidic devices.

Bioorthogonal Reaction-Mediated ELISA Using Peroxide Test Strip as Signal Readout for Point-of-Care Testing

2017 ◽  
Vol 89 (11) ◽  
pp. 6113-6119 ◽  
Author(s):  
Bei Ran ◽  
Yunlei Xianyu ◽  
Mingling Dong ◽  
Yiping Chen ◽  
Zhiyong Qian ◽  
...  
Keyword(s):  
Point Of Care ◽  
Test Strip ◽  
Point Of Care Testing ◽  
Bioorthogonal Reaction ◽  
Signal Readout

Giant Magnetoresistive Based Handheld System for Rapid Detection of Human NT-proBNP

2019 ◽  
Author(s):  
Wei Wang ◽  
Todd Klein ◽  
James Collins
Keyword(s):  
Limit Of Detection ◽  
Detection System ◽  
Point Of Care ◽  
High Sensitivity ◽  
Time Signal ◽  
Point Of Care Testing ◽  
Detection Range ◽  
Further Development ◽  
Signal Readout

In this work, we developed giant magnetoresistive (GMR) based handheld biosensing systems that serve as platform for detecting human NT-proBNP. This assay takes advantages of high sensitivity and real-time signal readout of GMR biosensor. The limit of detection was estimated to be less than 0.01ng/mL, and detection range covered from 0.01 ng/mL to 5 ng/mL was obtained. The assay can be completed within 20 min, which is very important for further development of point-of-care testing. The proposed GMR handheld system is also successfully used for the detection of real NT-proBNP human samples. It can be foreseen that this handheld detection system could become a robust contender in the applications of in vitro biomarker diagnostics.

Progress of Simple Signal Readout-based Point-of-Care Testing

2021 ◽  
Vol 49 (1) ◽  
pp. 1-13
Author(s):  
Hong-Hong RAO ◽  
Hai-Xia LIU ◽  
Ming-Yue LUO ◽  
Xin XUE ◽  
Wei Ming-Ming ◽  
...  
Keyword(s):  
Point Of Care ◽  
Point Of Care Testing ◽  
Signal Readout
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