scholarly journals Electrochemical Immunosensors Based on Screen-Printed Gold and Glassy Carbon Electrodes: Comparison of Performance for Respiratory Syncytial Virus Detection

Biosensors ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 175
Author(s):  
Wioleta Białobrzeska ◽  
Daniel Firganek ◽  
Maciej Czerkies ◽  
Tomasz Lipniacki ◽  
Marta Skwarecka ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Glassy Carbon ◽  
Dynamic Range ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Electrochemical Characterizations ◽  
Virus Detection ◽  
Electrochemical Immunosensors ◽  
Syncytial Virus ◽  
Screen Printed

This paper presents the development and comparison of label-free electrochemical immunosensors based on screen-printed gold and glassy carbon (GC) disc electrodes for efficient and rapid detection of respiratory syncytial virus (RSV). Briefly, the antibody specific to the F protein of RSV was successfully immobilized on modified electrodes. Antibody coupling on the Au surface was conducted via 4-aminothiophenol (4-ATP) and glutaraldehyde (GA). The GC surface was modified with poly-L-lysine (PLL) for direct anti-RSV conjugation after EDC/NHS (1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-Hydroxysuccinimide) activation. Electrochemical characterizations of the immunosensors were carried out by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). GC-based immunosensors show a dynamic range of antigen detection from 1.0 × 105 PFU/mL to 1.5×107 PFU/mL, more than 1.0 × 105 PFU/mL to 1.0 × 107 PFU/mL for the Au-based sensor. However, the GC platform is less sensitive and shows a higher detection limit (LOD) for RSV. The limit of detection of the Au immunosensor is 1.1 × 103 PFU/mL, three orders of magnitude lower than 2.85 × 106 PFU/mL for GC. Thus, the Au-based immunosensor has better analytical performance for virus detection than a carbon-based platform due to high sensitivity and very low RSV detection, obtained with good reproducibility.

scholarly journals Highly sensitive dual mode electrochemical platform for microRNA detection

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Pawan Jolly ◽  
Marina R. Batistuti ◽  
Anna Miodek ◽  
Pavel Zhurauski ◽  
Marcelo Mulato ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Dynamic Range ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Dual Mode ◽  
Electrode Surfaces ◽  
Microrna Detection ◽  
Highly Sensitive ◽  
Detection Mode ◽  
Amplification Strategy

Abstract MicroRNAs (miRNAs) play crucial regulatory roles in various human diseases including cancer, making them promising biomarkers. However, given the low levels of miRNAs present in blood, their use as cancer biomarkers requires the development of simple and effective analytical methods. Herein, we report the development of a highly sensitive dual mode electrochemical platform for the detection of microRNAs. The platform was developed using peptide nucleic acids as probes on gold electrode surfaces to capture target miRNAs. A simple amplification strategy using gold nanoparticles has been employed exploiting the inherent charges of the nucleic acids. Electrochemical impedance spectroscopy was used to monitor the changes in capacitance upon any binding event, without the need for any redox markers. By using thiolated ferrocene, a complementary detection mode on the same sensor was developed where the increasing peaks of ferrocene were recorded using square wave voltammetry with increasing miRNA concentration. This dual-mode approach allows detection of miRNA with a limit of detection of 0.37 fM and a wide dynamic range from 1 fM to 100 nM along with clear distinction from mismatched target miRNA sequences. The electrochemical platform developed can be easily expanded to other miRNA/DNA detection along with the development of microarray platforms.

scholarly journals A High-Response Electrochemical As(III) Sensor Using Fe3O4–rGO Nanocomposite Materials

Chemosensors ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 150
Author(s):  
Haibing Hu ◽  
Wenjie Lu ◽  
Xingnan Liu ◽  
Fancheng Meng ◽  
Jianxiong Zhu
Keyword(s):  
Electron Microscopy ◽  
Glassy Carbon ◽  
Metal Ion ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Detection Methods ◽  
Experimental Conditions ◽  
Water Field ◽  
Conductive Substrates ◽  
Higher Sensitivity

Nowadays, heavy metal ion pollution in water is becoming more and more common, especially arsenic, which seriously threatens human health. In this work, we used Fe3O4–rGO nanocomposites to modify a glassy carbon electrode and selected square wave voltametric electrochemical detection methods to detect trace amounts of arsenic in water. Field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM) showed that Fe3O4 nanoparticles were uniformly distributed on the rGO sheet, with a particle size of about 20 nm. Raman spectroscopy and electrochemical impedance spectroscopy (EIS) showed that rGO provides higher sensitivity and conductive substrates. Under optimized experimental conditions, Fe3O4–rGO-modified glassy carbon electrodes showed a higher sensitivity (2.15 µA/ppb) and lower limit of detection (1.19 ppb) for arsenic. They also showed good selectivity, stability, and repeatability.

Evaluation of Respiratory Syncytial Virus Detection by Rapid Antigen Tests in Childhood

2007 ◽  
Vol 219 (4) ◽  
pp. 212-216 ◽  
Author(s):  
U. Schauer ◽  
G. Ihorst ◽  
A. Rohwedder ◽  
G. Petersen ◽  
R. Berner ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Virus Detection ◽  
Syncytial Virus ◽  
Antigen Tests

A colorimetric immunoassay for respiratory syncytial virus detection based on gold nanoparticles–graphene oxide hybrids with mercury-enhanced peroxidase-like activity

2014 ◽  
Vol 50 (78) ◽  
pp. 11526-11528 ◽  
Author(s):  
Lei Zhan ◽  
Chun Mei Li ◽  
Wen Bi Wu ◽  
Cheng Zhi Huang
Keyword(s):  
Gold Nanoparticles ◽  
Graphene Oxide ◽  
Respiratory Syncytial Virus ◽  
Virus Detection ◽  
Colorimetric Immunoassay ◽  
Syncytial Virus

A novel colorimetric immunoassay for detection of respiratory syncytial virus (RSV) was developed based on mercury-stimulated peroxidase-like activity of gold nanoparticles–graphene composites (AuNPs–GO).

Respiratory and Enteric Virus Detection in Children

2016 ◽  
Vol 32 (1) ◽  
pp. 84-93 ◽  
Author(s):  
Marko Pokorn ◽  
Monika Jevšnik ◽  
Miroslav Petrovec ◽  
Andrej Steyer ◽  
Tatjana Mrvič ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Febrile Seizure ◽  
Viral Infections ◽  
Virus Detection ◽  
Enteric Virus ◽  
Febrile Seizures ◽  
Healthy Controls ◽  
Human Coronavirus ◽  
Detection Rates ◽  
Syncytial Virus

The majority of children with febrile seizures have viral infections and viruses were detected in 22% to 63% of children in published studies. Using molecular methods, viruses were also detected in asymptomatic persons. A prospective study was conducted to detect respiratory and enteric viruses in 192 children with febrile seizures and compare the detection rates to those found in 156 healthy age-matched controls. A respiratory or enteric virus was detected in 72.9% of children with febrile seizures and in 51.4% of healthy controls. The viruses most strongly associated with febrile seizures were influenza, respiratory syncytial virus, parainfluenza, human coronavirus, and rotavirus. Compared to healthy controls, the age-adjusted odds ratios for nasopharynx virus positivity in febrile seizure patients were 79.4, 2.8, 7.2, and 4.9 for influenza virus, parainfluenza virus, respiratory syncytial virus, and human coronavirus, respectively, and 22.0 for rotavirus in stool. The detected virus did not influence clinical features of febrile seizure.

scholarly journals A quite sensitive fluorescent loop-mediated isothermal amplification for rapid detection of respiratory syncytial virus

2019 ◽  
Vol 13 (12) ◽  
pp. 1135-1141 ◽  
Author(s):  
Yihong Hu ◽  
Zhenzhou Wan ◽  
Yonglin Mu ◽  
Yi Zhou ◽  
Jia Liu ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Rapid Detection ◽  
Limit Of Detection ◽  
Isothermal Amplification ◽  
Clinical Samples ◽  
High Morbidity ◽  
Rt Pcr ◽  
Loop Mediated Isothermal Amplification ◽  
Syncytial Virus ◽  
Syto 9

Introduction: Human respiratory syncytial virus (hRSV) is a common respiratory virus closely related to respiratory tract infection (RTI). Rapid and accurate detection of hRSV is urgently needed to reduce the high morbidity and mortality due to hRSV infection. Methodology: Here, we established a highly sensitive and specific reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for the rapid detection of A and B group hRSV simultaneously. The specific primer sets for hRSV A and B groups were designed in the M and M2-2 gene, respectively. SYTO 9 was used as the fluorescent dye for real-time monitoring of the amplification of hRSV RNA without cross reaction between hRSV A and B. Results: The limit of detection (LOD) of our new method was 281.17 50% tissue culture infective doses (TCID50)/mL for hRSV A and 1.58 TCID50/mL for hRSV B. Using 90 clinical samples, a comparison to traditional RT-PCR was performed to validate this assay. The positivity rate of RT-LAMP and RT-PCR were 67.8% and 55.6%, respectively, and the positivity rate of RT-LAMP was significantly higher than RT-PCR (χ2 test, P < 0.01). Conclusions: Compared with traditional RT-PCR method, the newly developed fluorescent RT-LAMP combined with well-designed primers and SYTO 9 is quite sensitive, specific, rapid and well applicable to hRSV clinical diagnosis.

scholarly journals Development and Standardization of a High-Throughput Multiplex Immunoassay for the Simultaneous Quantification of Specific Antibodies to Five Respiratory Syncytial Virus Proteins

mSphere ◽  
2019 ◽  
Vol 4 (2) ◽  
Author(s):  
Rutger M. Schepp ◽  
Cornelis A. M. de Haan ◽  
Deidre Wilkins ◽  
Hans Layman ◽  
Barney S. Graham ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
High Throughput ◽  
Large Scale ◽  
Dynamic Range ◽  
Valuable Insight ◽  
Conformational Structure ◽  
Multiplex Immunoassay ◽  
Rsv Infection ◽  
Syncytial Virus ◽  
Antibody Levels

ABSTRACT Human respiratory syncytial virus (RSV) is a major cause of severe respiratory disease in (premature) newborns and causes respiratory illness in the elderly. Different monoclonal antibody (MAb) and vaccine candidates are in development worldwide and will hopefully become available within the near future. To implement such RSV vaccines, adequate decisions about immunization schedules and the different target group(s) need to be made, for which the assessment of antibody levels against RSV is essential. To survey RSV antigen-specific antibody levels, we developed a serological multiplex immunoassay (MIA) that determines and distinguishes antibodies against the five RSV glycoproteins postfusion F, prefusion F, Ga, Gb, and N simultaneously. The standardized RSV pentaplex MIA is sensitive, highly reproducible, and specific for the five RSV proteins. The preservation of the conformational structure of the immunodominant site Ø of prefusion F after conjugation to the beads has been confirmed. Importantly, good correlation is obtained between the microneutralization test and the MIA for all five proteins, resulting in an arbitrarily chosen cutoff value of prefusion F antibody levels for seropositivity in the microneutralization assay. The wide dynamic range requiring only two serum sample dilutions makes the RSV-MIA a high-throughput assay very suitable for (large-scale) serosurveillance and vaccine clinical studies. IMPORTANCE In view of vaccine and monoclonal development to reduce hospitalization and death due to lower respiratory tract infection caused by RSV, assessment of antibody levels against RSV is essential. This newly developed multiplex immunoassay is able to measure antibody levels against five RSV proteins simultaneously. This can provide valuable insight into the dynamics of (maternal) antibody levels and RSV infection in infants and toddlers during the first few years of life, when primary RSV infection occurs.

Voltammetry Determination of Cefotaxime on Zinc Oxide Nanorod Modified Electrode

2020 ◽  
Vol 17 (1) ◽  
pp. 40-46
Author(s):  
Vanitha Vasantharaghavan ◽  
Ravichandran Cingaram
Keyword(s):  
Zinc Oxide ◽  
Glassy Carbon Electrode ◽  
Modified Electrode ◽  
Glassy Carbon ◽  
Dynamic Range ◽  
Limit Of Detection ◽  
Accurate Determination ◽  
Voltammetric Determination ◽  
Carbon Electrode

Background: The Glassy Carbon Electrode (GCE) was modified with zinc oxide nanoparticles to enhance the electrocatalytic activity of the redox behavior of cefotaxime ion. ATOMIC Force Microscopy (AFM) photographic studies showed the nanorod like structure of the zinc oxide, which was coated uniformly on the electrode surface. Methods: The zinc oxide nanorod modified electrode was used as novel voltammetric determination of cefotaxime. The results of voltammetric behavior are satisfactory in the electro oxidation of cefotaxime, and exhibit considerable improvement compared to glassy carbon electrode. Results: Under the optimized experimental conditions, the ZnO nanorod modified electrode exhibit better linear dynamic range from 300 ppb to 700 ppb with lower limit of detection 200 ppb for the stripping voltammetric determination of cefotaxime. Conclusion: The pharmaceutical and clinical formulation of cefotaxime was successfully applied for accurate determination of trace amounts on ZnO nanomateials modified electrode.

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