scholarly journals Development of Surface-Enhanced Raman Scattering (SERS)-Based Surface-Corrugated Nanopillars for Biomolecular Detection of Colorectal Cancer

Biosensors ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 163
Author(s):  
Kuan-Hung Chen ◽  
Meng-Ju Pan ◽  
Zoljargal Jargalsaikhan ◽  
Tseren-Onolt Ishdorj ◽  
Fan-Gang Tseng
Keyword(s):  
Colorectal Cancer ◽  
Raman Scattering ◽  
Hot Spots ◽  
Electrochemical Etching ◽  
Surface Enhanced Raman Scattering ◽  
Biomolecular Detection ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Comparable Concentration

In this paper, a nanobiosensor with surface-enhanced Raman scattering (SERS) capability is introduced for highly sensitive miRNA detection in colorectal cancer. This sensor was designed and fabricated by employing a nanoshielding mechanism from nanopolystyrene beads to resist reactive ion etching and allow anisotropic electrochemical etching, producing high-aspect-ratio, surface-corrugated nanopillars (SiNPs) on a silicon wafer to create extensive hot spots along the nanopillars for improved SERS signals. SERS enhancements were correlated with nanorange roughness, indicating that hot spots along the pillars were the crucial factor to improve the SERS effect. We achieved the detection capability of a trace amount of R6G (10−8 M), and the SERS signal enhancement factor (EF) was close to 1.0 × 107 on surface-corrugated gold SiNPs. miRNA samples were also demonstrated on this sensor with good sensitivity and specificity. The target molecule miR-21-Cy5 was easily monitored through Raman spectrum variation with a PCR-comparable concentration at around 100 pM with clear nucleotide-specific Raman signals, which is also suitable for biomolecule sensing.

Optimization of electromagnetic hot spots in surface-enhanced Raman scattering substrates for an ultrasensitive drug assay of emergency department patients’ plasma

The Analyst ◽  
2020 ◽  
Vol 145 (23) ◽  
pp. 7662-7672
Author(s):  
Thakshila Liyanage ◽  
Adrianna N. Masterson ◽  
Sumon Hati ◽  
Greta Ren ◽  
Nicholas E. Manicke ◽  
...  
Keyword(s):  
Emergency Department ◽  
Raman Scattering ◽  
Hot Spots ◽  
Surface Enhanced Raman Scattering ◽  
Ultrasensitive Detection ◽  
Drug Assay ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Emergency Department Patients

Nanoplasmonic superlattice surface-enhanced Raman scattering substrates have been developed for an ultrasensitive detection of fentanyl and cocaine from patients’ plasma.

Binary “island” shaped arrays with high-density hot spots for surface-enhanced Raman scattering substrates

Nanoscale ◽  
2018 ◽  
Vol 10 (29) ◽  
pp. 14220-14229 ◽  
Author(s):  
Weidong Zhao ◽  
Shuyuan Xiao ◽  
Yuxian Zhang ◽  
Dong Pan ◽  
Jiahui Wen ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Hot Spots ◽  
Surface Enhanced Raman Scattering ◽  
High Density ◽  
Self Assembled Monolayer ◽  
Sers Substrates ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Self Assembled

The BISA with high-density hot spots as reproducible SERS substrates by combining an opal structure with self-assembled monolayer AuNPs is demonstrated.

scholarly journals Formation of Interstitial Hot-Spots Using the Reduced Gap-Size between Plasmonic Microbeads Pattern for Surface-Enhanced Raman Scattering Analysis

Sensors ◽  
2019 ◽  
Vol 19 (5) ◽  
pp. 1046 ◽  
Author(s):  
Taeksu Lee ◽  
Sanghee Jung ◽  
Soongeun Kwon ◽  
Woochang Kim ◽  
Jinsung Park ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Hot Spots ◽  
Hybrid Approach ◽  
Chemical Deposition ◽  
Surface Enhanced Raman Scattering ◽  
Imprint Lithography ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Nano Imprint

To achieve an effective surface-enhanced Raman scattering (SERS) sensor with periodically distributed “hot spots” on wafer-scale substrates, we propose a hybrid approach combining physical nano-imprint lithography and a chemical deposition method to form a silver microbead array. Nano-imprint lithography (NIL) can lead to mass-production and high throughput, but is not appropriate for generating strong “hot-spots.” However, when we apply electrochemical deposition to an NIL substrate and the reaction time was increased to 45 s, periodical “hot-spots” between the microbeads were generated on the substrates. It contributed to increasing the enhancement factor (EF) and lowering the detection limit of the substrates to 4.40 × 106 and 1.0 × 10−11 M, respectively. In addition, this synthetic method exhibited good substrate-to-substrate reproducibility (RSD < 9.4%). Our research suggests a new opportunity for expanding the SERS application.

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