scholarly journals Electrochemical Immunosensor for Human IgE Using Ferrocene Self-Assembled Monolayers Modified ITO Electrode

Biosensors ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 38
Author(s):  
Myungsang Park ◽  
Yesol Song ◽  
Ki Jun Kim ◽  
Seung Jun Oh ◽  
Jun Ki Ahn ◽  
...  
Keyword(s):  
Indium Tin Oxide ◽  
Dynamic Range ◽  
Immunoglobulin E ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Self Assembled Monolayers ◽  
Limit Of Quantification ◽  
Ito Electrode ◽  
Assembled Monolayers ◽  
Self Assembled

The immunoglobulin E (IgE) level in serum is an important factor in the examination of allergy. Ferrocene (Fc)-modified self-assembled monolayers (SAMs) were placed on an indium tin oxide (ITO) electrode as a sensing layer for the detection of human IgE. The Fc moiety in the SAMs facilitated the electron transfer through the organic SAMs layer and electrocatalytic signal amplification. The electrochemical measurement was accomplished after the sandwich type immobilization of the receptor antibody, target human IgE, and enzyme conjugated secondary antibody. The enzyme product, p-aminophenol, was quantitatively analyzed by redox cycling via Fc. In addition, the electrochemical impedance spectroscopy (EIS) was investigated for the detection of IgE. The limit of detection (LOD), limit of quantification (LOQ), and dynamic range of the electrochemical sensor were 3 IU/mL, 10 IU/mL, and from 10 IU/mL to 100 IU/mL, respectively.

scholarly journals Microfluidic Impedance Biosensor Chips Using Sensing Layers Based on DNA-Based Self-Assembled Monolayers for Label-Free Detection of Proteins

Biosensors ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 80
Author(s):  
Khaled Alsabbagh ◽  
Tim Hornung ◽  
Achim Voigt ◽  
Sahba Sadir ◽  
Taleieh Rajabi ◽  
...  
Keyword(s):  
Troponin I ◽  
Electrochemical Impedance ◽  
Specific Binding ◽  
Self Assembled Monolayers ◽  
Significant Shift ◽  
Label Free ◽  
Label Free Detection ◽  
Assembled Monolayers ◽  
Self Assembled ◽  
Biosensor Chip

A microfluidic chip for electrochemical impedance spectroscopy (EIS) is presented as bio-sensor for label-free detection of proteins by using the example of cardiac troponin I. Troponin I is one of the most specific diagnostic serum biomarkers for myocardial infarction. The microfluidic impedance biosensor chip presented here consists of a microscope glass slide serving as base plate, sputtered electrodes, and a polydimethylsiloxane (PDMS) microchannel. Electrode functionalization protocols were developed considering a possible charge transfer through the sensing layer, in addition to analyte-specific binding by corresponding antibodies and reduction of nonspecific protein adsorption to prevent false-positive signals. Reagents tested for self-assembled monolayers (SAMs) on gold electrodes included thiolated hydrocarbons and thiolated oligonucleotides, where SAMs based on the latter showed a better performance. The corresponding antibody was covalently coupled on the SAM using carbodiimide chemistry. Sampling and measurement took only a few minutes. Application of a human serum albumin (HSA) sample, 1000 ng/mL, led to negligible impedance changes, while application of a troponin I sample, 1 ng/mL, led to a significant shift in the Nyquist plot. The results are promising regarding specific detection of clinically relevant concentrations of biomarkers, such as cardiac markers, with the newly developed microfluidic impedance biosensor chip.

Preparation and Characterization of Self-Assembled Monolayers on Indium Tin Oxide

Langmuir ◽  
2000 ◽  
Vol 16 (15) ◽  
pp. 6208-6215 ◽  
Author(s):  
C. Yan ◽  
M. Zharnikov ◽  
A. Gölzhäuser ◽  
M. Grunze
Keyword(s):  
Tin Oxide ◽  
Indium Tin Oxide ◽  
Self Assembled Monolayers ◽  
Assembled Monolayers ◽  
Self Assembled

Studies on the Effect of Solvents on Self-Assembled Monolayers Formed from Organophosphonic Acids on Indium Tin Oxide

Langmuir ◽  
2012 ◽  
Vol 28 (25) ◽  
pp. 9487-9495 ◽  
Author(s):  
Xin Chen ◽  
Erwann Luais ◽  
Nadim Darwish ◽  
Simone Ciampi ◽  
Pall Thordarson ◽  
...  
Keyword(s):  
Tin Oxide ◽  
Indium Tin Oxide ◽  
Self Assembled Monolayers ◽  
Effect Of Solvents ◽  
Assembled Monolayers ◽  
Self Assembled

scholarly journals Corrosion Protection of AA7075 Aluminium Alloy by Trimethoxy-Silanes Self-Assembled Monolayers

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Rodrigo S. Neves ◽  
Daiane P. B. Silva ◽  
Artur J. Motheo
Keyword(s):  
Aluminium Alloy ◽  
Electrochemical Impedance ◽  
Protective Coatings ◽  
Reduction Reaction ◽  
Self Assembled Monolayers ◽  
Organic Monolayers ◽  
Assembled Monolayers ◽  
Self Assembled ◽  
Surface Modified ◽  
Trimethoxy Silane

This study presents electrochemical data concerning the aluminium alloy AA7075 surface modified by self-assembled monolayers (SAMs) of octadecyl-trimethoxy-silane and propyl-trimethoxy-silane. Polarisation curves have shown SAMs blocking effect, as they partially block the oxygen reduction reaction and displace the corrosion potential to positive values. Electrochemical impedance spectroscopy experiments have suggested that the protective effect comes from the oxide layer stabilization by the organic monolayers, which block the corroding species diffusion to the surface. These results show the potential of using methoxy-silanes SAMs as corrosion protective coatings; however, they are also indicative of the superior octadecyl-trimethoxy-silane protective characteristics.

Biointerfaces on Indium–Tin Oxide Prepared from Organophosphonic Acid Self-Assembled Monolayers

Langmuir ◽  
2014 ◽  
Vol 30 (28) ◽  
pp. 8509-8515 ◽  
Author(s):  
Muthukumar Chockalingam ◽  
Astrid Magenau ◽  
Stephen G. Parker ◽  
Maryam Parviz ◽  
S. R. C. Vivekchand ◽  
...  
Keyword(s):  
Tin Oxide ◽  
Indium Tin Oxide ◽  
Self Assembled Monolayers ◽  
Assembled Monolayers ◽  
Self Assembled
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