scholarly journals FMSP-Nanoparticles Induced Cell Death on Human Breast Adenocarcinoma Cell Line (MCF-7 Cells): Morphometric Analysis

Biomolecules ◽  
2018 ◽  
Vol 8 (2) ◽  
pp. 32 ◽  
Author(s):  
Firdos Khan ◽  
Sultan Akhtar ◽  
Sarah Almofty ◽  
Dana Almohazey ◽  
Munthar Alomari
Keyword(s):  
Cell Death ◽  
Cell Line ◽  
Morphometric Analysis ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Adenocarcinoma Cell Line ◽  
Adenocarcinoma Cell ◽  
Human Breast Adenocarcinoma ◽  
Mcf 7

scholarly journals Pit-1/GHF-1 and GH expression in the MCF-7 human breast adenocarcinoma cell line

2002 ◽  
Vol 173 (1) ◽  
pp. 161-167 ◽  
Author(s):  
C Gil-Puig ◽  
M Blanco ◽  
T Garcia-Caballero ◽  
C Segura ◽  
R Perez-Fernandez
Keyword(s):  
Cell Proliferation ◽  
Cell Line ◽  
Carcinoma Cell ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Rt Pcr ◽  
Adenocarcinoma Cell Line ◽  
Adenocarcinoma Cell ◽  
Human Breast Adenocarcinoma ◽  
Mcf 7

GH expression in mammary tumors has been related to the increase and spreading of cell proliferation. Using the MCF-7 human breast adenocarcinoma cell line, it has been demonstrated that autocrine GH-stimulated mammary carcinoma cell proliferation decreased the apoptosis rate and enhanced cell spreading. Surprisingly, no data are available about the presence of Pit-1 (the main pituitary regulator of GH) or GH expression in this cell line. Using RT-PCR, Western blot and immunohistochemistry, we have demonstrated the presence of both mRNA coding Pit-1 and GH as well as Pit-1 and GH protein in the MCF-7 cell line. These data could imply that Pit-1 may be an adequate target to inhibit breast cell proliferation.

scholarly journals Cytotoxicity of Manganese (III) Complex in Human Breast Adenocarcinoma Cell Line Is Mediated by the Generation of Reactive Oxygen Species Followed by Mitochondrial Damage

2016 ◽  
Vol 35 (6) ◽  
pp. 672-682 ◽  
Author(s):  
Qudes Al-anbaky ◽  
Zeiyad Al-karakooly ◽  
Surya P. Kilaparty ◽  
Megha Agrawal ◽  
Yahya M. Albkuri ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Death ◽  
Breast Adenocarcinoma ◽  
Oxygen Species ◽  
Human Breast ◽  
Adenocarcinoma Cell Line ◽  
Cytotoxic Effects ◽  
Adenocarcinoma Cell ◽  
Human Breast Adenocarcinoma ◽  
Mcf 7

Manganese (Mn) complexes are widely studied because of their important catalytic properties in synthetic and biochemical reactions. A Mn (III) complex of an amidoamine ligand was synthesized using a tetradentate amidoamine ligand. In this study, the Mn (III) complex was evaluated for its biological activity by measuring its cytotoxicity in human breast adenocarcinoma cell line (MCF-7). Cytotoxic effects of the Mn (III) complex were determined using established biomarkers in an attempt to delineate the mechanism of action and the utility of the complex as a potential anticancer drug. The Mn (III) complex induces cell death in a dose- and time-dependent manner as shown by microculture tetrazolium assay, a measure of cytotoxic cell death. Our results demonstrated that cytotoxic effects were significantly increased at higher concentrations of Mn (III) complex and with longer time of treatment. The IC50 (Inhibitor concentration that results in 50% cell death) value of Mn (III) complex in MCF-7 cells was determined to be 2.5 mmol/L for 24 hours of treatment. In additional experiments, we determined the Mn (III) complex–mediated cell death was due to both apoptotic and nonspecific necrotic cell death mechanisms. This was assessed by ethidium bromide/acridine orange staining and flow cytometry techniques. The Mn (III) complex produced reactive oxygen species (ROS) triggering the expression of manganese superoxide dismutase 1 and ultimately damaging the mitochondrial function as is evident by a decline in mitochondrial membrane potential. Treatment of the cells with free radical scavenger, N, N-dimethylthiourea decreased Mn (III) complex–mediated generation of ROS and attenuated apoptosis. Together, these results suggest that the Mn (III) complex–mediated MCF-7 cell death utilizes combined mechanism involving apoptosis and necrosis perhaps due to the generation of ROS.

scholarly journals Lycopene-rich extract from red guava ( Psidium guajava L.) displays cytotoxic effect against human breast adenocarcinoma cell line MCF-7 via an apoptotic-like pathway

2018 ◽  
Vol 105 ◽  
pp. 184-196 ◽  
Author(s):  
Raimunda C. dos Santos ◽  
Alicia S. Ombredane ◽  
Jéssica Maria T. Souza ◽  
Andreanne G. Vasconcelos ◽  
Alexandra Plácido ◽  
...  
Keyword(s):  
Cell Line ◽  
Cytotoxic Effect ◽  
Psidium Guajava ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Adenocarcinoma Cell Line ◽  
Adenocarcinoma Cell ◽  
Human Breast Adenocarcinoma ◽  
Mcf 7
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