scholarly journals Binding of Sperm to the Zona Pellucida Mediated by Sperm Carbohydrate-Binding Proteins is not Species-Specific in Vitro between Pigs and Cattle

Biomolecules ◽  
2013 ◽  
Vol 3 (4) ◽  
pp. 85-107 ◽  
Author(s):  
Kazuya Takahashi ◽  
Kazuhiro Kikuchi ◽  
Yasuomi Uchida ◽  
Saeko Kanai-Kitayama ◽  
Reiichiro Suzuki ◽  
...  
Keyword(s):  
Zona Pellucida ◽  
Binding Proteins ◽  
Carbohydrate Binding ◽  
Carbohydrate Binding Proteins ◽  
Species Specific

Effect of microenvironment and cell-line type on carbohydrate-binding proteins of macrophage-like cells

1988 ◽  
Vol 66 (11) ◽  
pp. 1169-1176 ◽  
Author(s):  
Hans-Joachim Gabius ◽  
Katalin Vehmeyer
Keyword(s):  
Binding Proteins ◽  
Cultured Cells ◽  
Sodium Dodecyl ◽  
Carbohydrate Binding ◽  
Intercellular Interaction ◽  
Cell Extracts ◽  
Carbohydrate Binding Proteins ◽  
Sugar Receptors

The pattern of sugar inhibition of rosette formation, a model for intercellular interaction between cultured cells and glutaraldehyde-fixed, trypsinated rabbit erythrocytes, served to infer the presence of carbohydrate-binding proteins. This profile from cell extracts for the two murine macrophage-like cell lines, P388D1 and J774A.1, was comparatively analyzed by affinity chromatography on supports with immobilized carbohydrates (lactose, L-fucose, N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, and maltose) or with the immobilized mannose-rich yeast glycoprotein mannan or fetuin-derived glycopeptides containing sialic acid residues. After elution with specific sugar in the absence of Ca2+ ions, the proteins were separated by sodium dodecyl sulfate – polyacrylamide slab gel electrophoresis. The composition of carbohydrate-binding proteins of the two lines clearly exhibited quantitative and qualitative differences. Moreover, the pattern of P388D1 cells was also demonstrated to change significantly in response to alterations in the conditions of the physiological environment. These alterations were imposed by in vitro growth, by subsequent in vivo growth in nude mice, and by re-adaptation of cells to culture after in vivo passage. Collectively, our observations and other physiological and biochemical reports on macrophage lectins indicate that the presence of sugar receptors with different specificities may be an indicator of macrophage differentiation, being reversibly modulated to a considerable extent by external factors, e.g., microenvironment. Extensive but selective alterations in this respect could play an important role in the control of recognition and effector mechanisms within diverse functions of macrophage subpopulations.

Inhibition of sperm-egg fusion in the hamster and mouse by carbohydrates

Zygote ◽  
1994 ◽  
Vol 2 (3) ◽  
pp. 253-262 ◽  
Author(s):  
Ruben H. Ponce ◽  
Umbert A. Urch ◽  
Ryuzo Yanagimachi
Keyword(s):  
Extracellular Matrix ◽  
Plasma Membrane ◽  
Zona Pellucida ◽  
Binding Proteins ◽  
Toxic Effects ◽  
Carbohydrate Binding ◽  
Dextran Sulphate ◽  
Membrane Glycoproteins ◽  
Carbohydrate Binding Proteins

SummaryAfter spermatozoa bind to and penetrate the extracellular matrix of the egg, the zona pellucida, they adhere to and fuse with the plasma membrane of the egg. Since sperm–egg fusion may involve membrane glycoproteins and/or carbohydrate binding proteins, we sought to test this hypothesis by challenging sperm–egg fusion in hamster and in mouse with added carbohydrates. In this study, a number of carbohydrate and glycoconjugates were examined for their ability to inhibit sperm–eggfusion. In the hamster, D(+)-glucosamine, D(+)-galactosamine, albumin-bovine-glucosamide and-galactosamide, fucoidan and dextran sulphate inhibited the fusion of spermatozoa with zona-free eggs. The same effects were seen in the mouse, except for the toxic effects of D(+)-galactosamine. These facts suggest a role of carbohydrate binding proteins or glycoproteins in the fertilisation process at the level of binding to and fusing with the oolemma.

In Vitro Synthesis of Carbohydrate-Binding Proteins by Human Leucocytes

1986 ◽  
Vol 15 (2) ◽  
pp. 123-138 ◽  
Author(s):  
Howard J. Allen ◽  
Dave Kachurek ◽  
Steve Bulera ◽  
Edward Kisailus ◽  
Richard Dicioccio
Keyword(s):  
Binding Proteins ◽  
Carbohydrate Binding ◽  
In Vitro Synthesis ◽  
Carbohydrate Binding Proteins

scholarly journals Screening for carbohydrate-binding proteins in extracts of Uruguayan plants

2003 ◽  
Vol 36 (7) ◽  
pp. 851-860 ◽  
Author(s):  
A. Plá ◽  
E. Alonso ◽  
F. Batista-Viera ◽  
L. Franco Fraguas
Keyword(s):  
Binding Proteins ◽  
Carbohydrate Binding ◽  
Carbohydrate Binding Proteins
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