scholarly journals Comparison of Anticancer Activity of Dorycnium pentaphyllum Extract on MCF-7 and MCF-12A Cell Line: Correlation with Invasion and Adhesion

Biomolecules ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 671
Author(s):  
Gözde Koygun ◽  
Emine Arslan ◽  
Gökhan Zengin ◽  
Giustino Orlando ◽  
Claudio Ferrante
Keyword(s):  
Breast Cancer ◽  
Lipid Peroxidation ◽  
Cell Line ◽  
Cytotoxic Effect ◽  
Bioinformatics Analysis ◽  
Biological Activities ◽  
Xtt Assay ◽  
Cytotoxic Effects ◽  
Antiproliferative Effects ◽  
Mcf 7

Dorycnium pentaphyllum subsp. haussknechtii is an important medicinal plant in several countries, including Turkey. This study aimed to evaluate the cytotoxicity of a crude extract of D. pentaphyllum subsp. haussknechtii against different breast cell lines to determine invasion, adhesion, and lipid peroxidation. The cytotoxic effects on MCF-7 breast cancer and MCF-12A as the immortalized cell line were examined by the XTT assay. Invasion and adhesion studies were performed according to the manufacturer’s kit procedure to IC50 values for 48 h. Lipid peroxidation was measured in the MCF-7 cell. A bioinformatics analysis was conducted to unravel the mechanism of action underlying antiproliferative effects, as well. According to XTT results, the tested extract showed a time- and a concentration-dependent cytotoxic effect. The most effective concentration was 100.5 µg/mL (48 h), which was selected for biological activities, such as apoptotic activity, invasion, adhesion, and lipid peroxidation assays. The extract caused tumoral cell death, and it did not have a cytotoxic effect on healthy human breast cells. Duplication times and measurement of CI analyses of cells were performed using the real-time cell analysis system xCELLigence. Finally, the bioinformatics analysis indicated the prominent role of quercetin as an extract component exerting a key role in the observed antiproliferative effects. This was supported by the micromolar/submicromolar affinity of quercetin towards proto-oncogene serine/threonine–protein kinase (PIM-1) and hematopoietic cell kinase (HCK), both involved in breast cancer. Altogether, our findings proposed that the extraction of the plant can be an effective strategy to isolate biomolecules with promising cytotoxic effects against breast cancer cells.

scholarly journals The Cytotoxic Effects of Humic Acid on Human Breast Cancer Cells

Proceedings ◽  
2018 ◽  
Vol 2 (25) ◽  
pp. 1565
Author(s):  
Asli Aykac ◽  
Eda Becer ◽  
Tuğçe Balcı Okcanoğlu ◽  
Meryem Güvenir ◽  
Kaya Süer ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Humic Acid ◽  
Cell Line ◽  
Cytotoxic Effect ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Cytotoxic Effects ◽  
Human Breast Adenocarcinoma ◽  
Mcf 7

Breast cancer is the most common cancer among women and in order to create alternative treatments different types of in vivo and in vitro studies have used various plant-based therapeutic agents. Humic acid (HA) induces apoptosis and has various pharmacological properties including anti-inflammatory and anti-proliferative effects. In our study, we examined the cytotoxic effects of HA at concentrations of 5, 10, 20, 50 and 100 μg/mL in human breast adenocarcinoma MCF-7 cell line for 24 and 48 h. By using MTT method, it has been found out that HA 100 g/mL had cytotoxic effect on human breast adenocarcinoma MCF-7 cell line at both 24 and 48 h; also found out that the effective dose of HA at the same time (24 and 48 h) was 50 μg/mL. The results of our study will shed light on the development of alternative therapeutic approaches in the treatment of cancer by evaluating the cytotoxic effect of HA.

scholarly journals Annona muricataLeaf Extract Triggered Intrinsic Apoptotic Pathway to Attenuate Cancerous Features of Triple Negative Breast Cancer MDA-MB-231 Cells

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Jee Young Kim ◽  
Thien T. P. Dao ◽  
Kwangho Song ◽  
Sait Byul Park ◽  
Hyeri Jang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Triple Negative Breast Cancer ◽  
Triple Negative ◽  
Biological Activities ◽  
Intrinsic Apoptotic Pathway ◽  
Apoptotic Pathway ◽  
Tropical Regions ◽  
Mcf 7

Annona muricataL., known as graviola, is an evergreen plant of the tropical regions and is a rich source of natural products. Graviola has various biological activities, and it is best known for its anticancer activity. This study aimed to investigate the effects of crude graviola extractin vitroon breast cancer cells; in particular, we aimed to identify an agent against triple negative breast cancer (TNBC). We used the TNBC MDA-MB-231 cell line as the experimental model and the ER(+) non-TNBC MCF-7 breast cancer cell line as the control. We identified annonaceous acetogenins, including annonacin isomers, characteristic to this plant by using liquid chromatography tandem mass spectrometry (LC/MS/MS). We observed a significant decrease in the cell viability in both cell lines within 48 h, whereas impaired cell motility and invasiveness were observed only in the MDA-MB-231 cell line. While the MCF-7 cells showed an ER-dependent mechanism of apoptosis, the apoptosis of MDA-MB-231 cells was governed by an intrinsic apoptotic pathway triggered by graviola leaf extract (GLE).

Role of β-Estradiol in MCF-7 Breast Cancer Cell Line Based on the Bioinformatics Analysis

2018 ◽  
Vol 84 (3) ◽  
pp. 268-276
Author(s):  
Rong  Wang ◽  
Jinbin  Li ◽  
Chunyu  Yin ◽  
Di  Zhao ◽  
Yulan  Zhao ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Bioinformatics Analysis ◽  
Cancer Cell Line ◽  
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Influence of anchoring moieties on new benzimidazole-based Schiff base copper(ii) complexes towards estrogen dependent breast cancer cells

2021 ◽  
Vol 50 (10) ◽  
pp. 3701-3716
Author(s):  
Anup Paul ◽  
Priya Singh ◽  
Maxim L. Kuznetsov ◽  
Anirban Karmakar ◽  
M. Fátima C. Guedes da Silva ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Schiff Base ◽  
Cancer Cells ◽  
Cell Line ◽  
Breast Cancer Cells ◽  
Cytotoxic Effect ◽  
Line P ◽  
Mcf 7

Effects of triphenylphosphonium and triethylammonium linkers on new benzimidazole based Schiff base copper(ii) complexes are described. A triphenylphosphonium anchored compound exhibits a better cytotoxic effect than cisplatin on the MCF-7 cell line.

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