scholarly journals Deeper Insights on Alchornea cordifolia (Schumach. & Thonn.) Müll.Arg Extracts: Chemical Profiles, Biological Abilities, Network Analysis and Molecular Docking

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 219
Author(s):  
Kouadio Ibrahime Sinan ◽  
Gunes Ak ◽  
Ouattara Katinan Etienne ◽  
József Jekő ◽  
Zoltán Cziáky ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Carcinoma Cells ◽  
Human Hepatocellular Carcinoma ◽  
Total Phenolic ◽  
Hepatocellular Carcinoma Cells ◽  
Methanolic Extracts ◽  
Human Hepatocellular Carcinoma Cells ◽  
Phenolic And Flavonoid

Alchornea cordifolia (Schumach. & Thonn.) Müll. Arg. is a well-known African medicinal plant traditionally used for various healing purposes. In the present study, methanolic, ethyl acetate and infusion extracts of A. cordifolia leaves were studied for their total phenolic and flavonoid contents and screened for their chemical composition. Moreover, the enzyme (acetyl- and butyryl-cholinesterases, α-amylase, α-glucosidase, and tyrosinase) inhibitory and cytotoxicity activities on HepG2: human hepatocellular carcinoma cells, B16 4A5: murine melanoma cells, and S17: murine bone marrow (normal) cells of extracts were evaluated. Finally, components-targets and docking analyzes were conducted with the aim to unravel the putative mechanisms underlying the observed bio-pharmacological effects. Interestingly, the infusion and methanolic extracts showed significantly higher total phenolic and flavonoid contents compared with the ethyl acetate extract (TPC: 120.38–213.12 mg GAE/g and TFC: 9.66–57.18 mg RE/g). Besides, the methanolic extracts followed by the infusion extracts were revealed to contain a higher number of compounds (84 and 74 compounds, respectively), while only 64 compounds were observed for the ethyl acetate extract. Gallic acid, ellagic acid, shikimic acid, rutin, quercetin, myricetin, vitexin, quercitrin, kaempferol, and naringenin were among the compounds that were commonly identified in all the studied extracts. Additionally, the methanolic and infusion extracts displayed higher antioxidant capacity than ethyl acetate extract in all assays performed. In ABTS and DPPH radical scavenging assays, the methanol extract (500.38 mg TE/g for DPPH and 900.64 mg TE/g for ABTS) exhibited the best ability, followed by the water and ethyl acetate extracts. Furthermore, the extracts exhibited differential enzyme inhibitory profiles. In particular, the methanolic and infusion extracts showed better cytotoxic selectivity activity against human hepatocellular carcinoma cells. Overall, this study demonstrated A cordifolia to be a species worthy of further investigations, given its richness in bioactive phytochemicals and wide potentialities for antioxidants and pharmacological agents.

scholarly journals In VitroProtective Effects ofLycium barbarumBerries Cultivated in Umbria (Italy) on Human Hepatocellular Carcinoma Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
M. R. Ceccarini ◽  
S. Vannini ◽  
S. Cataldi ◽  
M. Moretti ◽  
M. Villarini ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Carcinoma Cells ◽  
Human Hepatocellular Carcinoma ◽  
Migration And Invasion ◽  
Total Phenolic ◽  
Lycium Barbarum ◽  
Hepatocellular Carcinoma Cells ◽  
Umbria Region ◽  
Increased Risk ◽  
Human Hepatocellular Carcinoma Cells

Lycium barbarumis a famous plant in the traditional Chinese medicine. The plant is known to have health-promoting bioactive components. The properties ofLycium barbarumberries cultivated in Umbria (Italy) and their effect on human hepatocellular carcinoma cells (HepG2) have been investigated in this work. The obtained results demonstrated that theLycium barbarumberries from Umbria region display high antioxidant properties evaluated by total phenolic content and ORAC method, on hydrophilic and lipophilic fractions. Moreover, on HepG2 cell lineLycium barbarumberries extract did not change cell viability analyzed by MTT and Trypan blue exclusion assay and did not induce genotoxic effect analyzed by comet assay. Furthermore, it was demonstrated, for the first time, that the berries extract showed a protective effect on DNA damage, expressed as antigenotoxic activityin vitro. Finally,Lycium barbarumberries extract was able to modulate the expression of genes involved in oxidative stress, proliferation, apoptosis, and cancer. In particular, downexpression of genes involved in tumor migration and invasion (CCL5), in increased risk of metastasis and antiapoptotic signal (DUSP1), and in carcinogenesis (GPx-3 and PTGS1), together with overexpression of tumor suppressor gene (MT3), suggested that UmbrianLycium barbarumberries could play a protective role against hepatocellular carcinoma.

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