scholarly journals Kaempferitrin-Treated HepG2 Differentially Expressed Exosomal Markers and Affect Extracellular Vesicle Sizes in the Secretome

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 187
Author(s):  
Wei-Chi Ku ◽  
Badrinathan Sridharan ◽  
Jiann-Yeu Chen ◽  
Jen-Ying Li ◽  
Shu-Yu Yang ◽  
...  
Keyword(s):  
Lipid Profile ◽  
Conditioned Medium ◽  
High Frequency ◽  
Cancer Cell Line ◽  
Extracellular Vesicle ◽  
Diabetic Rats ◽  
Protein Expression Profile ◽  
Differential Protein Expression ◽  
Force Microscopy ◽  
Atomic Force

Kaempferitrin is extracted in significantly high quantities from the leaves of Cinnamomum osmophloeum, which belongs to a group of plant species that comes under the genus Cinnamomum, well-known for its established anti-diabetic property in Chinese medicine. Oral administration of kaempferitrin and Cinnamomum osmophloeum extract reduced blood sugar in alloxan-induced diabetic rats and improved the lipid profile in hamsters respectively. In this paper we studied the differential protein expression profile using mass spectrometry approach in the kaempferitrin-treated conditioned medium of liver cancer cell line HepG2. We discovered that 33 genes were up/down-regulated consistently between two biological samples. A slightly different version of the analysis software selected 28 genes, and the final 18 genes that appeared in both lists were selected. Interestingly, 5 proteins out of 18 were either exosomal markers or reported in high frequency of occurrence in exosome/secreted vesicles. We also examined the extracellular particles with atomic force microscopy (AFM), which showed that the conditioned medium of kaempferitrin treated had larger vesicles and fewer small vesicles. Expression of some lipid-regulating genes were also altered. Our data suggested that extracellular vesicle secretions may be regulated by kaempferitrin, and regulation of lipid profile by kampeferitrin involves multiple mechanisms.

Study of NSCLC cell migration promoted by NSCLC-Derived Extracellular Vesicle using atomic force microscopy

2021 ◽  
Author(s):  
Shuwei Wang ◽  
Jiajia Wang ◽  
Tuoyu Ju ◽  
Kaige Qu ◽  
Fan Yang ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Migration ◽  
Cancer Cells ◽  
Extracellular Vesicles ◽  
Extracellular Vesicle ◽  
Cancer Microenvironment ◽  
Biological Processes ◽  
Force Microscopy ◽  
Atomic Force ◽  
Cellular Components

Extracellular Vesicles (EVs) secreted by cancer cells have a key role in the cancer microenvironment and progression. Previous studies have mainly focused on molecular functions, cellular components and biological processes...

scholarly journals Self-driving capacitive cantilevers for high-frequency atomic force microscopy

2012 ◽  
Vol 100 (5) ◽  
pp. 053110 ◽  
Author(s):  
Keith A. Brown ◽  
Benjamin H. Yang ◽  
R. M. Westervelt
Keyword(s):  
Atomic Force Microscopy ◽  
High Frequency ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Extending applications of AFM to fluidic AFM in single living cell and extracellular vesicle studies

2021 ◽  
Author(s):  
Yuan Qiu ◽  
Chen-Chi Chien ◽  
Basilis Maroulis ◽  
Angelo Gaitas ◽  
Bin Gong
Keyword(s):  
Atomic Force Microscopy ◽  
Single Cell ◽  
Living Cell ◽  
Cell Monolayer ◽  
Extracellular Vesicle ◽  
Liquid Environment ◽  
Force Microscopy ◽  
Atomic Force ◽  
Single Living Cell ◽  
Single Living

Abstract In this article, a review of the application of atomic force microscopy (AFM) for the analyses of extracellular vesicles is presented. This information is then extended to include fluidic Atomic Force Microscopy (fluidic AFM) applications. Fluidic AFM is an offshoot of AFM that combines a microfluidic cantilever with AFM and has enabled the research community to conduct biological, pathological, and pharmacological studies on cells at the single-cell level in a liquid environment. AFM applications involving single cell and extracellular vesicle studies, colloidal force spectroscopy, and single cell adhesion measurements are discussed. In this review, new results are offered, using fluidic AFM, to illustrate (1) the speed with which sequential measurements of adhesion using coated colloid beads can be done, (2) the ability to assess lateral binding forces (LBFs) of endothelial or epithelial cells in a confluent cell monolayer in appropriate physiological environment, and (3) the ease of measurement of vertical binding force (VBFs) of intercellular adhesion between heterogeneous cells. Finally, key applications are discussed that include extracellular vesicle absorption, manipulation of a single living cell by intracellular injection, sampling of cellular fluid from a single living cell, patch clamping, and mass measurements of a single living cell.

Microwave absorption of polymer nanocomposites on the base high-density polyethylene and magnetite nanoparticles

2018 ◽  
Vol 51 (2) ◽  
pp. 130-142 ◽  
Author(s):  
MA Ramazanov ◽  
FV Hajiyeva ◽  
AM Maharramov ◽  
HM Mamedov
Keyword(s):  
Polymer Nanocomposites ◽  
High Frequency ◽  
High Density Polyethylene ◽  
Electromagnetic Waves ◽  
Nanocomposite Materials ◽  
Nanocomposite Films ◽  
Frequency Range ◽  
Force Microscopy ◽  
Atomic Force ◽  
Agglomeration Of Nanoparticles

In this article, we report about the synthesis of magnetic polymer nanocomposites comprising magnetite (Fe3O4) nanoparticles in a polyethylene (PE) matrix. The structure and composition of nanocomposite materials using scanning electron microscopy and atomic force microscopy have been studied. It has been defined that the agglomeration of nanoparticles does not occur, by increasing the volume content of nanoparticles up to 60%, and Fe3O4 nanoparticles act out like single-domain particles. It has also been revealed that nanocomposites based on PE + Fe3O4 with nanoparticle content in a polymer matrix up to 60% behave like superparamagnetic particles. It has also been revealed that the PE + Fe3O4-based nanocomposites effectively absorb the electromagnetic waves in the high frequency range of 0.1–30 GHz. It is shown that the properties of ultrahigh electromagnetic waves can be varied by varying the thickness of the nanocomposite films and the concentration of Fe3O4 nanoparticles.

scholarly journals Electrothermally driven high-frequency piezoresistive SiC cantilevers for dynamic atomic force microscopy

2014 ◽  
Vol 116 (5) ◽  
pp. 054304 ◽  
Author(s):  
R. Boubekri ◽  
E. Cambril ◽  
L. Couraud ◽  
L. Bernardi ◽  
A. Madouri ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
High Frequency ◽  
Force Microscopy ◽  
Atomic Force
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