scholarly journals Cell-Free Approaches and Therapeutic Biomolecules for Cardiac Regeneration

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 161
Author(s):  
Mariann Gyöngyösi
Keyword(s):  
Stem Cells ◽  
Human Heart ◽  
Cardiac Regeneration ◽  
Cardiac Stem Cells ◽  
Regeneration Capacity ◽  
Cardiac Progenitors ◽  
Human Organs ◽  
Adult Human

In contrast with some adult human organs, such as liver or skin, the adult human heart shows very limited self-regeneration capacity, attributed to the negligible presence of resident cardiac stem cells or cardiac progenitors [...]

scholarly journals P2551The distribution and characteristics of endogenous cardiac stem cells in the atria and ventricle of the adult human heart

2017 ◽  
Vol 38 (suppl_1) ◽  
Author(s):  
S. Sarvananthan ◽  
P.P. Punjabi ◽  
F. Lewis ◽  
N. Latif ◽  
P. Sarathchandra ◽  
...  
Keyword(s):  
Stem Cells ◽  
Human Heart ◽  
Cardiac Stem Cells ◽  
Adult Human

scholarly journals Generation of Cardiomyocytes From Vascular Adventitia-Resident Stem Cells

2018 ◽  
Vol 123 (6) ◽  
pp. 686-699 ◽  
Author(s):  
Subba Rao Mekala ◽  
Philipp Wörsdörfer ◽  
Jochen Bauer ◽  
Olga Stoll ◽  
Nicole Wagner ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Heart Failure ◽  
Stem Cells ◽  
Endothelial Cells ◽  
Aortic Wall ◽  
Fluorescent Protein ◽  
Therapeutic Option ◽  
Cardiac Regeneration ◽  
Cardiac Progenitors ◽  
Resident Stem Cells

Rationale: Regeneration of lost cardiomyocytes is a fundamental unresolved problem leading to heart failure. Despite several strategies developed from intensive studies performed in the past decades, endogenous regeneration of heart tissue is still limited and presents a big challenge that needs to be overcome to serve as a successful therapeutic option for myocardial infarction. Objective: One of the essential prerequisites for cardiac regeneration is the identification of endogenous cardiomyocyte progenitors and their niche that can be targeted by new therapeutic approaches. In this context, we hypothesized that the vascular wall, which was shown to harbor different types of stem and progenitor cells, might serve as a source for cardiac progenitors. Methods and Results: We describe generation of spontaneously beating mouse aortic wall-derived cardiomyocytes without any genetic manipulation. Using aortic wall-derived cells (AoCs) of WT (wild type), αMHC (α-myosin heavy chain), and Flk1 (fetal liver kinase 1)-reporter mice and magnetic bead-associated cell sorting sorting of Flk1 + AoCs from GFP (green fluorescent protein) mice, we identified Flk1 + CD (cluster of differentiation) 34 + Sca-1 (stem cell antigen-1)-CD44 − AoCs as the population that gives rise to aortic wall-derived cardiomyocytes. This AoC subpopulation delivered also endothelial cells and macrophages with a particular accumulation within the aortic wall-derived cardiomyocyte containing colonies. In vivo, cardiomyocyte differentiation capacity was studied by implantation of fluorescently labeled AoCs into chick embryonic heart. These cells acquired cardiomyocyte-like phenotype as shown by αSRA (α-sarcomeric actinin) expression. Furthermore, coronary adventitial Flk1 + and CD34 + cells proliferated, migrated into the myocardium after mouse myocardial infarction, and expressed Isl-1 + (insulin gene enhancer protein-1) indicative of cardiovascular progenitor potential. Conclusions: Our data suggest Flk1 + CD34 + vascular adventitia-resident stem cells, including those of coronary adventitia, as a novel endogenous source for generating cardiomyocytes. This process is essentially supported by endothelial cells and macrophages. In summary, the therapeutic manipulation of coronary adventitia-resident cardiac stem and their supportive cells may open new avenues for promoting cardiac regeneration and repair after myocardial infarction and for preventing heart failure.

scholarly journals Blood Serum Stimulates p38-Mediated Proliferation and Changes in Global Gene Expression of Adult Human Cardiac Stem Cells

Cells ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 1472 ◽  
Author(s):  
Anna L. Höving ◽  
Kazuko E. Schmidt ◽  
Madlen Merten ◽  
Jassin Hamidi ◽  
Ann-Katrin Rott ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
P38 Mapk ◽  
Regulatory Networks ◽  
Human Blood Plasma ◽  
Cardiac Stem Cells ◽  
Dependent Manner ◽  
Protective Effects ◽  
Adult Human ◽  
Age And Sex

During aging, senescent cells accumulate in various tissues accompanied by decreased regenerative capacities of quiescent stem cells, resulting in deteriorated organ function and overall degeneration. In this regard, the adult human heart with a generally low regenerative potential is of extreme interest as a target for rejuvenating strategies with blood borne factors that might be able to activate endogenous stem cell populations. Here, we investigated for the first time the effects of human blood plasma and serum on adult human cardiac stem cells (hCSCs) and showed significantly increased proliferation capacities and metabolism accompanied by a significant decrease of senescent cells, demonstrating a beneficial serum-mediated effect that seemed to be independent of age and sex. However, RNA-seq analysis of serum-treated hCSCs revealed profound effects on gene expression depending on the age and sex of the plasma donor. We further successfully identified key pathways that are affected by serum treatment with p38-MAPK playing a regulatory role in protection from senescence and in the promotion of proliferation in a serum-dependent manner. Inhibition of p38-MAPK resulted in a decline of these serum-mediated beneficial effects on hCSCs in terms of decreased proliferation and accelerated senescence. In summary, we provide new insights in the regulatory networks behind serum-mediated protective effects on adult human cardiac stem cells.

Harnessing endogenous intra- and extra-cardiac stem cells for cardiac regeneration – hope or hype?

2009 ◽  
Vol 6 (4) ◽  
pp. 127-133 ◽  
Author(s):  
Sunny Sun-Kin Chan ◽  
Ying-Zhang Shueh ◽  
Yen-Wen Liu ◽  
Patrick C.H. Hsieh
Keyword(s):  
Stem Cells ◽  
Cardiac Regeneration ◽  
Cardiac Stem Cells

Medical imaging for cardiac regeneration using resident cardiac stem cells

2013 ◽  
Vol 5 (5) ◽  
pp. 477-486
Author(s):  
Sung Hyun Choi ◽  
So Young Yoo ◽  
Sae Mi Yoo ◽  
Kyeong Been Lee ◽  
Sang Hong Baek ◽  
...  
Keyword(s):  
Stem Cells ◽  
Medical Imaging ◽  
Cardiac Regeneration ◽  
Cardiac Stem Cells
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