scholarly journals Characterization of Bacterial Cellulose-Based Wound Dressing in Different Order Impregnation of Chitosan and Collagen

Biomolecules ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1511
Author(s):  
Khatarina Meldawati Pasaribu ◽  
Saharman Gea ◽  
Syafruddin Ilyas ◽  
Tamrin Tamrin ◽  
Izabela Radecka
Keyword(s):  
Bacterial Cellulose ◽  
Wound Dressing ◽  
Antibacterial Properties ◽  
Tissue Growth ◽  
Hydroxyl Group ◽  
Sem Images ◽  
E Coli ◽  
Zones Of Inhibition ◽  
Collagen Molecules

Bacterial cellulose (BC), chitosan (Chi), and collagen (Col) are known as biopolymers which have met some properties that are required as wound dressing. This study focused on investigating the fabrication of BC-based wound dressing with chitosan and collagen, since chitosan has red blood cells binding and anti-bacterial properties, while collagen can support cell and tissue growth for skin wounds. The BC-based wound dressing was prepared by impregnating BC fibers in the chitosan and/or collagen solution for 24 h. FTIR was used to confirm the intermolecular interaction of amine and hydroxyl group of chitosan and/or collagen in BC-based wound dressing. Furthermore, the XRD diffractogram of the wound dressing show broader peaks at 14.2°, 16.6°, and 22.4° due to the presence of chitosan and collagen molecules in BC fibers. These results were then supported by SEM images which confirmed that chitosan and collagen were well penetrated into BC fibers. TGA curves revealed that BC/Chi/Col has better thermal properties based on the Tmax compare to BC/Col/Chi. Feasibility of the mats to be applied as wound dressing was also supported by other tests, i.e., water content, porosity, and hemocompatibility, which indicates that the wound dressing is classified as nonhemolytic materials. However, BC/Col/Chi was considered a more potential wound dressing to be applied compared to BC/Chi/Col since it has larger pores and showed better antibacterial properties (larger zones of inhibition) against S. aureus and E. coli via disk diffusion tests.

scholarly journals NOVEL COMPOSITE OF SILVER-BACTERIAL CELLULOSE (Ag-BC) FROM SIWALAN SAP (Borassus flabellifer) AND ITS ANTIBACTERIAL ACTIVITY

2020 ◽  
Vol 83 (1) ◽  
pp. 19-25
Author(s):  
Suntini Suntini ◽  
Anastasia Wheni Indrianingsih ◽  
Harjono Harjono
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Cellulose ◽  
Antibacterial Properties ◽  
Reduction Process ◽  
Diffusion Method ◽  
Natural Material ◽  
Gram Positive Bacteria ◽  
Antibacterial Assay ◽  
Natural Composite

Recently, a wound healing from natural composite with excellent properties is in a high demand. In this study, a novel composite of bacterial cellulose made from Siwalan sap (Borassus flabellifer) was achieved. Siwalan is a common plant in Java Island of Indonesia and the application is very limited for beverage only. This study aims to determine the effect of the AgNO3/NaBH4 concentration ratio in the development of Ag-BC composites and its antibacterial properties from Siwalan sap. Ag-BC composites were prepared by impregnating the silver solution into the BC matrix through the reduction process with NaBH4. Characterization of Ag-BC composites conducted using Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and Energy Dispersive X-ray (EDX). Antibacterial assay was performed using disc diffusion method against Salmonella typhimurium (Gram-negative) and Staphylococcus aureus (Gram-positive) bacteria. The characterization of Ag-BC composite shows the nanostructure of BC with a length of fiber around 35-60 nm in width. The SEM-EDX micrograph showed that silver particles were impregnated into the BC matrix. Antibacterial activity test results showed that the Ag-BC composite had the ability to inhibit the bacteria S. typhimurium and S. aureus with good inhibition. This result showed the potential application of Ag-BC composite from Siwalan plant as a natural material for medical and pharmaceutical purpose, especially as an antibacterial agent.

scholarly journals Functional and structural basis of E. coli enolase inhibition by SF2312: a mimic of the carbanion intermediate

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Jolanta Krucinska ◽  
Michael N. Lombardo ◽  
Heidi Erlandsen ◽  
Akram Hazeen ◽  
Searle S. Duay ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
Antibacterial Properties ◽  
High Energy ◽  
Structural Basis ◽  
Gram Negative Bacteria ◽  
E Coli ◽  
Promising Target ◽  
Antibiotic Discovery ◽  
Growth And Reproduction

AbstractMany years ago, the natural secondary metabolite SF2312, produced by the actinomycete Micromonospora, was reported to display broad spectrum antibacterial properties against both Gram-positive and Gram-negative bacteria. Recent studies have revealed that SF2312, a natural phosphonic acid, functions as a potent inhibitor of human enolase. The mechanism of SF2312 inhibition of bacterial enolase and its role in bacterial growth and reproduction, however, have remained elusive. In this work, we detail a structural analysis of E. coli enolase bound to both SF2312 and its oxidized imide-form. Our studies support a model in which SF2312 acts as an analog of a high energy intermediate formed during the catalytic process. Biochemical, biophysical, computational and kinetic characterization of these compounds confirm that altering features characteristic of a putative carbanion (enolate) intermediate significantly reduces the potency of enzyme inhibition. When SF2312 is combined with fosfomycin in the presence of glucose-6 phosphate, significant synergy is observed. This suggests the two agents could be used as a potent combination, targeting distinct cellular mechanism for the treatment of bacterial infections. Together, our studies rationalize the structure-activity relationships for these phosphonates and validate enolase as a promising target for antibiotic discovery.

Effect of Synthesized Ag Nanoparticles by Using the Different Amounts of Polyvinylpyrrolidone for Ag-Natural Rubber Hybrid Sheets and their Antibacterial Properties

2017 ◽  
Vol 751 ◽  
pp. 270-276 ◽  
Author(s):  
Warot Prasanseang ◽  
Chaval Sriwong ◽  
Kittisak Choojun
Keyword(s):  
Natural Rubber ◽  
Low Cost ◽  
Antibacterial Properties ◽  
Rubber Matrix ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Zones Of Inhibition ◽  
Ft Ir ◽  
The Mean

Ag-natural rubber (Ag-NR) hybrid sheets were successfully prepared with a very simple and low cost method. In this method, silver nanoparticles (AgNPs) were firstly synthesized by a rapid and green microwave-assisted using polyvinylpyrroridone (PVP) media. The effect of PVP weight ratios towards the size of AgNPs was also investigated. Then, Ag-NR hybrid sheet samples were prepared by latex mixing-casting method using concentrated natural rubber (NR) latex with the synthesized AgNPs colloids. The characteristic absorption, particles sizes and shapes of the obtained AgNPs were examined through UV-vis, TEM and SAED. Also, the prepared Ag-NR sheet samples were characterized using XRD, FT-IR, SEM and EDS techniques. It was found that the particles sizes of all the synthesized AgNPs had spherical-like shape, and the mean sizes were increased from 29.7 to 90.4 nm upon increasing PVP contents. EDS results showed the AgNPs were well-dispersed and impregnated into the rubber matrix. Moreover, the antibacterial properties of the prepared Ag-NR sheets were tested by agar disk-diffusion method with Gram-positive and Gram-negative bacteria as Staphylococcus aureus(S. aureus) and Escherichia coli(E. coli), respectively. The results showed that the hybrid sheets exhibited excellent antibacterial properties against these bacteria, in which the zones of inhibition were also dependent on the synthesized AgNPs by utilizing the different amounts of PVP.

Bacterial cellulose-MMTs nanoreinforced composite films: novel wound dressing material with antibacterial properties

Cellulose ◽  
2012 ◽  
Vol 20 (2) ◽  
pp. 589-596 ◽  
Author(s):  
Mazhar Ul-Islam ◽  
Taous Khan ◽  
Waleed Ahmad Khattak ◽  
Joong Kon Park
Keyword(s):  
Bacterial Cellulose ◽  
Wound Dressing ◽  
Composite Films ◽  
Antibacterial Properties

Preparation and Characterization of a Bio-Composites Scaffold Containing Nano-Hydroxyapatite /Carboxymethyl Chitosan

2012 ◽  
Vol 476-478 ◽  
pp. 2055-2058 ◽  
Author(s):  
Zhi Hua Lu ◽  
Dong Mei Zhao ◽  
Kang Ning Sun
Keyword(s):  
Mechanical Properties ◽  
Freeze Drying ◽  
Crystallization Process ◽  
Average Diameter ◽  
Carboxymethyl Chitosan ◽  
Hydroxyl Group ◽  
Sem Images ◽  
Homogenous Distribution ◽  
Potential Use

In this study, a genipin cross linked scaffold containing nano-hydroxyapatite (n-HAp) and carboxymehthyl chitosan (CMCS) was developed by freeze drying technique. The scaffolds were characterized using FTIR, XRD and SEM. The cytotoxicity of the scaffolds was compared with scaffolds cross linked by glutaraldehyde, and the Young’s modulus was also tested. FTIR and XRD results indicated that CMCS’s hydroxyl group, amino and amide regulated the n-HAp crystallization process, which results in the nano homogenous distribution of n-HAp and provided nano topographical features for nanohybrid scaffolds. SEM images revealed the scaffolds had porous structure and the pores were interconnected with an average diameter of 150 μm, which was profit for the growth of tissues. Cell morphology showed the genipin cross linked scaffolds had less toxicity and more facility for adhesion and proliferation of cells. Great mechanical properties of the scaffolds indicate their potential use in bone tissue engineering.

Fabrication and Characterization of Hydroxyapatite Nanocomposites Based on Bacterial Cellulose with Surface Modification

2012 ◽  
Vol 476-478 ◽  
pp. 457-460 ◽  
Author(s):  
Na Yin ◽  
Shi Yan Chen ◽  
Yang Ou Yang ◽  
Lian Tang ◽  
Zhe Li ◽  
...  
Keyword(s):  
Surface Modification ◽  
Bacterial Cellulose ◽  
Three Dimensional ◽  
Biomimetic Synthesis ◽  
Sem Images ◽  
Xrd Pattern ◽  
Fabrication And Characterization ◽  
Sbf Solution ◽  
Great Potential Application

In this study, biomimetic synthesis of hydroxyapatite (HAp) nanocomposites based on bacterial cellulose (BC) with sodium alginate (SA) surface modification was studied. The results showed that SA treatment promoted the apatite nucleation ability of BC in SBF solution. The FE-SEM images demonstrated that HAp nanoparticles were successfully formed in the three dimensional nanofiber network of BC. FTIR spectra indicated that the obtained HAp crystals were partially substituted with carbonate, which were very similar with natural bones. It was also found that both of crystallite size and crystallinity of HAp crystals observed from XRD pattern were very low. The resultant nanocomposites consisted of nano-scale HAp crystals and SA treated BC nanofibrous have great potential application in bone tissue engineering.

scholarly journals Preparation and Characterization of Potato Starch Film with Various Size of Nano-SiO2

Polymers ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 1172 ◽  
Author(s):  
Rongfei Zhang ◽  
Xiangyou Wang ◽  
Meng Cheng
Keyword(s):  
Mechanical Properties ◽  
Food Packaging ◽  
Water Resistance ◽  
Gas Permeability ◽  
Potato Starch ◽  
Antibacterial Properties ◽  
E Coli ◽  
Nano Sio2 ◽  
Starch Films

The various sizes (15, 30, 80, and 100 nm) of nano-SiO2/potato starch films were synthesized and characterized. The gas permeability, antibacterial properties, and mechanical properties of the films were evaluated to their potential for application as food packaging materials. Results indicated that the 100 nm nano-SiO2 was well dispersed in the starch matrix, which induced an active group on the surface of 100 nm nano-SiO2 adequately combined with starch macromolecule. The water resistance and mechanical properties of the films were improved with the addition of nano-SiO2. Notably, resistance to ultraviolet and thermal aging was also enhanced. The nano-SiO2/potato starch films were more efficient against Escherichia coli (E. coli) than Staphylococcus aureus (S. aureus). Remarkable preservation properties of the films packaging the white mushrooms were obtained, with those of the 100 nm films considered superior. This study can significantly guide the rational choice of the nano-SiO2 size to meet the packaging requirements of various agricultural products.

scholarly journals Biopolymer Composites with Ti/Au Nanostructures and Their Antibacterial Properties

Pharmaceutics ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 826
Author(s):  
Petr Slepička ◽  
Dominik Fajstavr ◽  
Markéta Krejčová ◽  
Silvie Rimpelová ◽  
Nikola Slepičková Kasálková ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Large Scale ◽  
Antibacterial Properties ◽  
Chemical Properties ◽  
Laser Confocal Microscopy ◽  
Cathode Sputtering ◽  
X Ray ◽  
E Coli ◽  
Atomic Force

In this study, we have aimed at the preparation and characterization of poly-l-lactic acid (PLLA) composites with antibacterial properties. Thin bilayers of titanium and gold of various thickness ratios were deposited on PLLA by a cathode sputtering method; selected samples were subsequently thermally treated. The surface morphology of the prepared composites was studied by atomic force, scanning electron, and laser confocal microscopy. The chemical properties of the composites were determined by X-ray photoelectron and energy-dispersive X-ray spectroscopy in combination with contact angle and zeta potential analyses. The antibacterial properties of selected samples were examined against a Gram-negative bacterial strain of E. coli. We have found that a certain combination of Au and Ti nanolayers in combination with heat treatment leads to the formation of a unique wrinkled pattern. Moreover, we have developed a simple technique by which a large-scale sample modification can be easily produced. The dimensions of wrinkles can be tailored by the sequence and thickness of the deposited metals. A selected combination of gold, titanium, and heat treatment led to the formation of a nanowrinkled pattern with excellent antibacterial properties.

Synthesis and Characterization of Cellulose/Quaternary Phosphonium Salt

2013 ◽  
Vol 842 ◽  
pp. 138-141 ◽  
Author(s):  
Fan Long Kong ◽  
Jun Song ◽  
Bo Wen Cheng ◽  
Yun Bo Zheng
Keyword(s):  
Phosphonium Salt ◽  
Antibacterial Property ◽  
Antibacterial Properties ◽  
Synthesis And Characterization ◽  
Cellulose Structure ◽  
E Coli ◽  
Mechanical Stirring ◽  
H Nmr ◽  
Quaternary Phosphonium Salt

This paper investigates imparting antibacterial properties to wood pulp cellulose via introducing quaternary phosphonium salt groups in/onto the cellulose structure to get biocidal material. The 4-carboxybutyltriphenylphosphonium bromide was grafted to cellulose in an esterification at 70°C with mechanical stirring 5h.The grafted cellulose were evaluated with FTIR and 1H NMR. The product shows good antibacterial property against E. coli.

scholarly journals Comparative In vitro Antibacterial Properties of Methanol Extracts and Fractions of Myristica fragrans Seed and Thymus vulgaris Leaf

2021 ◽  
pp. 47-58
Author(s):  
Romanus A. Umoh ◽  
Affiong C. Essien ◽  
Imoh I. Johnny ◽  
Nsima A. Andy ◽  
Anwanabasi E. Udoh ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Antibacterial Properties ◽  
Absolute Methanol ◽  
Diffusion Method ◽  
Thymus Vulgaris ◽  
Gram Positive ◽  
E Coli ◽  
Methanol Extracts ◽  
Myristica Fragrans ◽  
Zones Of Inhibition

The aim of this work was to compare the antibacterial properties of methanol extracts and fractions of Myristica fragrans seed and Thymus vulgaris leaf on the gram positive and negative bacteria.  The Myristica fragrans seeds were crushed, defatted and air-dried. The defatted seed and leaf powders were separately macerated in absolute methanol for 72 hours. The methanol extracts and fractions were reconstituted at different concentrations of 100mg/mL, 80mg/mL, 60mg/mL, 40mg/mL and 20mg/mL for the antibacterial assay by agar diffusion method with activated cultured Staphylococcus aureus and Escherichia coli , incubated at 37oC for 24 hours . The results showed that these plants possess antibacterial activity on the basis of their zones of inhibition. Methanol extract of M. fragrans had a higher activity of 8-19mm on S. aureus than E. coli with 5-14mm range respectively. Ethylacetate fraction had the highest activity with 9-25mm on S. aureus, while chloroform fraction had the highest activity on E. coli with 8-18mm.  For T. vulgaris, the methanol extract had a higher activity of 6-18mm on E. coli than S. aureus of 4-17mm and for the fractions, n-hexane fraction had the highest activity of 7-20mm on S. aureus , while aqueous fraction had the highest activity of 5-18mm on E. coli, compared with zones of inhibition of 18mm against S. aureus  and 28mm against E. coli  for gentamycin of 2mg/mL which was the reference drug. Methanol extracts and fractions of M. fragrans seed and T. vulgaris leaf showed excellent activities on the gram positive and gram negative bacteria but the M. fragrans had a better activity than T. vulgaris.

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