scholarly journals In Vitro and In Vivo Antioxidant Activity of Agave sisalana Agro-Industrial Residue

Biomolecules ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1435 ◽  
Author(s):  
Stella Maria Andrade Gomes Barreto ◽  
Cesar Orlando Muñoz Cadavid ◽  
Rafael Amir de Oliveira Moura ◽  
Giovanna Melo Martins Silva ◽  
Samara Vitória Ferreira de Araújo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Direct Action ◽  
Radical Scavenging ◽  
Reducing Power ◽  
New Production ◽  
Industrial Residue ◽  
Agave Sisalana

Agave sisalana agro-industrial residue has considerable potential against damage associated with oxidative stress and skin aging. This study aims to demonstrate, in vitro and in vivo, the potential of Agave sisalana agro-industrial residue as a safe and effective alternative for the prevention of damage caused by oxidative stress and aging. The antioxidant activity was evaluated in vitro (total antioxidant capacity, reducing power, DPPH radical scavenging, metal chelating (Fe2+ and Cu2+), and hydroxyl radical scavenging) and in vivo using the Caenorhabditis elegans organism model. The extract showed in vitro antioxidant activity in all tests performed. Tests with C. elegans showed that the extract was able to reduce the intracellular levels of reactive oxygen species (ROS) and increase the survival rate of worms. A downregulation of gst-4::GFP expression suggests a direct action against free radicals. Agave sisalana agro-industrial residue extract (AsRE) can therefore be considered as a source of antioxidant biomolecules, and the use of this agro-industrial residue in a new production process can lead to sustainability and socioeconomic development.

scholarly journals In Vitro and In Vivo Antioxidant Activity of the New Magnetic-Cerium Oxide Nanoconjugates

Nanomaterials ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 1565 ◽  
Author(s):  
Turin-Moleavin ◽  
Fifere ◽  
Lungoci ◽  
Rosca ◽  
Coroaba ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Iron Oxide ◽  
Cerium Oxide ◽  
Chemical Activation ◽  
Radical Scavenging ◽  
Cerium Oxide Nanoparticles ◽  
Oxide Nanoparticles

Background. Cerium oxide nanoparticles present the mimetic activity of superoxide dismutase, being able to inactivate the excess of reactive oxygen species (ROS) correlated with a large number of pathologies, such as stents restenosis and the occurrence of genetic mutations that can cause cancer. This study presents the synthesis and biological characterisation of nanoconjugates based on nanoparticles of iron oxide interconnected with cerium oxide conjugates. Methods. The synthesis of magnetite-nanoceria nanoconjugates has been done in several stages, where the key to the process is the coating of nanoparticles with polyethyleneimine and its chemical activation-reticulation with glutaraldehyde. The nanoconjugates are characterised by several techniques, and the antioxidant activity was evaluated in vitro and in vivo. Results. Iron oxide nanoparticles interconnected with cerium oxide nanoparticles were obtained, having an average diameter of 8 nm. Nanoconjugates prove to possess superparamagnetic properties and the saturation magnetisation varies with the addition of diamagnetic components in the system, remaining within the limits of biomedical applications. In vitro free-radical scavenging properties of nanoceria are improved after the coating of nanoparticles with polyethylenimine and conjugation with magnetite nanoparticles. In vivo studies reveal increased antioxidant activity in all organs and fluids collected from mice, which demonstrates the ability of the nanoconjugates to reduce oxidative stress. Conclusion. Nanoconjugates possess magnetic properties, being able to scavenge free radicals, reducing the oxidative stress. The combination of the two properties mentioned above makes them excellent candidates for theranostic applications.

scholarly journals Optimization of Phenolic Compound Extraction from Chinese Moringa oleifera Leaves and Antioxidant Activities

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Beibei Zhao ◽  
Jiawen Deng ◽  
Hua Li ◽  
Yaqiang He ◽  
Tao Lan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Moringa Oleifera ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ultrasonic Extraction ◽  
Extraction Temperature ◽  
Moringa Oleifera Leaves

Rich in phenolic compounds, Moringa oleifera leaf extract (ME) exhibits significant antioxidant activity both in vitro and in vivo. ME has already been widely used in fields of medicine, functional food, and cosmetics. Ultrasonic extraction (UE) method has been improved to be one of the most effective ways to extract phenols from M. oleifera leaves. The purpose of this study was to optimize ultrasonic extraction of phenols by response surface methodology (RSM). Four parameters were discussed, such as ethanol concentration, solvent-sample ratio, extraction temperature, and extraction time. Also, purification methods of the crude ME by organic solvent extraction and column chromatography were examined. Antioxidant activities of ME and each fraction were evaluated by DPPH, ABTS, and hydroxy radical-scavenging activities and reducing power. The phenol content of the purified ME reached up to 962.6 mg RE/g, extremely higher than the crude extract 107.22 ± 1.93 mg RE/g. The antioxidant activity of the purified ME was also significantly improved. Furthermore, phenols were identified by using the HPLC-MS method, and the results showed that there were 6 phenolic acids and derivatives and 7 flavonoids in ME. Quercetin-3-O-β-D-glucoside isolated from ME showed excellent DPPH and ABTS radical-scavenging abilities, which were comparable to VC.

Review on Methods Used to Determine Antioxidant Activity of Origanum majorana

2021 ◽  
Vol 17 ◽  
Author(s):  
Gyanendra Narayan Mohapatra ◽  
Bimala Tripathy ◽  
B.V.V. Ravi Kumar ◽  
Bimalendu Chowdhury ◽  
Rajaram Das
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Radical Scavenging ◽  
Thiobarbituric Acid ◽  
Antioxidants Activity ◽  
Origanum Majorana ◽  
Online Library

Background: Presence of free radicals in human body are harmful and cell systems are induce many diseases like cardiovascular, diabetes, cancer, inflammation, neuro-degenerative disorder, atherosclerosis, cataract, etc. Antioxidants can balance the effect of free radicals. Antioxidant-rich herbs are identified for preparation of drugs that can be administered to neutralize the free radicals. In the present context the selected medicinal plant is “Origanum majorana” (Family- Lamiaceae) widely known as Marwa in India sub-continent. As the medical professionals show their desires towards composite or lateral treatment, application of drugs with herbal origin gained its importance. Objective: This review presented various in vitro and in vivo methods used in the antioxidant activity study of O.majorana and observed its efficacy to reduce oxidative stress. Methods: Referred many reliable sources like Research gate, PubMed, Science Direct, Google scholar, Wiley online library, books to collect all information about the antioxidant activity of the selected plant. Results: Used several methods to determine the antioxidants activity of O.majorana, such as superoxide radical scavenging, ferric ion reducing antioxidant potency, thiobarbituric acid reactive substances, hydrogen peroxide scavenging, hydroxyl radical scavenging, lipid peroxidation inhibition, etc. The selected plant contains many phytoconstituents such as gallic acid, ferulic acid, apigenin, catechin, rutin, quercetin, luteolin, linolenic acid, β-sitosterol, and essential oils, which may be responsible for antioxidant activity. Conclusions : The review article provides information for investigating and developing new antioxidant methods and major phytoconstituents from O.majorana for better therapy of oxidative stress-mediated complications.

scholarly journals In VitroAntioxidant, Antibacterial, and Cytotoxic Activity andIn VivoEffect ofSyngonium podophyllumandEichhornia crassipesLeaf Extracts on Isoniazid Induced Oxidative Stress and Hepatic Markers

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Shashank Kumar ◽  
Ramesh Kumar ◽  
Astha Dwivedi ◽  
Abhay K. Pandey
Keyword(s):  
Oxidative Stress ◽  
Metal Ion ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Brain Homogenate ◽  
Salmonella Typhi ◽  
Malondialdehyde Content ◽  
Rat Tissue

The present study reports thein vitroantioxidant, antibacterial, and cytotoxic potential ofSyngonium podophyllum(SP) andEichhornia crassipes(EC) leaf aqueous extracts as well as theirin vivoeffect on oxidative stress and hepatic biomarkers in isoniazid induced rats. Phytochemical screening of extracts revealed the presence of flavonoids, terpenoids, reducing sugars, alkaloids, and saponins. Phenolic content in SP and EC extracts was5.36±0.32and10.63±0.13 mg PGE/g, respectively, while flavonoid content was1.26±0.03and0.51±0.03 μg QE/mg, respectively. EC extract exhibited comparatively better antioxidant activity as indicated by reducing power (0.197–0.775), DPPH radical scavenging potential (11%–96%), and metal ion chelating ability (42%–93%). Both the extracts provided 13%–65% protection against lipid peroxidation in rat tissue (liver, kidney, and brain) homogenate. SP and EC extracts exhibited 51% and 43% cytotoxicity against lung cancer (NCI-H322) cell line, respectively. Both extracts demonstrated considerable antibacterial activity againstProteus vulgaris,Salmonella typhi, andBordetella bronchiseptica. Coadministration ofE. crassipesextract with isoniazid in rats accounted for 46% decrease in malondialdehyde content and 21% increase in FRAP value of plasma. It also mitigated the isoniazid induced alterations in serum enzymes (SGOT, SGPT, and ALP), total bilirubin, creatinine, and hemoglobin contents.S. podophyllumextract was found to be hepatotoxic.

scholarly journals Antioxidant Activities of Stilbenoids fromRheum emodiWall

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Yuan-yuan Chai ◽  
Fang Wang ◽  
Yan-li Li ◽  
Ke Liu ◽  
Hui Xu
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Inflammatory Diseases ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Hydroxyl Group ◽  
Antioxidant Power

Rheum emodiWall has been reported to possess protective effect in many inflammatory diseases and oxidative stress-related injuries. This study aims to investigate antioxidant power of stilbenoids fromR. emodiand then explore the material basis for its antioxidant potential. The most abundant stilbenoid piceatannol-4′-O-β-D-glucopyranoside (PICG) and its aglycon piceatannol (PICE) were isolated fromR. emodirhizome. Using well-accepted antioxidant chemicals as reference, antioxidant activity of these stilbenoids was examined by measuring DPPH and superoxide anion radical scavenging, ferric reducing power, and inhibition of lipid peroxidationin vitro. Both PICG and PICE displayed promising antioxidant activity in all the four assays. Comparisons among the tested compounds indicated that PICE has the most potent antioxidant activity and the presence of 3′-hydroxyl group may enhance antioxidant activity of stilbenoids. The antioxidative effect of PICE at the cellular level was further demonstrated on the model of hydrogen-peroxide-induced H9c2 rat cardiomyoblasts injury. Taking into account the rapidin vivometabolic transformation of PICG into PICE it can be inferred that the most abundant stilbenoid PICG may be an important constituent responsible for the antioxidant potential ofR. emodiand promising to be developed as an antioxidant agent for supplementary or therapeutic use.

scholarly journals Ethanolic Extract of Glycyrrhiza glabra to Ameliorate Oxidative Stress – Studies in vitro

2020 ◽  
Vol 2 (1) ◽  
pp. 80
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Glycyrrhiza Glabra ◽  
New Drugs ◽  
Phytochemical Analysis ◽  
Radical Scavenging Assay

Plants are major sources of bioactive organic molecules that are of interest to the pharmaceutical industry and are being screened for new drugs and chemicals. Glycyrrhiza glabra Linn. (Family: Fabaceae) also known as Liquorice, Mulaithi, or Yashtimadu is a well-known medicinal plant used in traditional medicine. Its roots and rhizomes are the medicinal parts used and are reported to possess antitumor, antimicrobial, antiviral, anti-inflammatory, immunoregulatory activities. This plant is also used as a flavoring agent due to its sweetness. In the present work, the ethanolic extract of Glycyrrhiza glabra was prepared, and its phytochemical analysis was done using HPLC. The in vitro antioxidant assays such as DPPH radical scavenging assay, Hydroxyl radical scavenging assay, total antioxidant activity assay, and total reducing power assay were done, and the results showed significant antioxidant activity of the extract. The extract was analyzed further to evaluate the ability to protect against oxidative stress in chicken liver tissue. The levels of glutathione and lipid peroxidation in H2O2 and/or G glabra extract-treated tissue indicated the potential to protect against oxidative stress under in vitro conditions. The in vitro comet assay results showed that the G glabra extract protected against H2O2 induced cellular DNA damage. These findings indicated promising antioxidant and antigenotoxic potential of G.glabra and need further exploration for translating these findings to its possible health benefits.

scholarly journals Characterization and Antioxidant Activity Determination of Neutral and Acidic Polysaccharides from Panax Ginseng C. A. Meyer

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 791 ◽  
Author(s):  
Hyung Min Kim ◽  
Yanxue Song ◽  
Gyu Hwan Hyun ◽  
Nguyen Phuoc Long ◽  
Jeong Hill Park ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Panax Ginseng ◽  
High Performance ◽  
Biological Effects ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
White Ginseng

Panax ginseng (P. ginseng) is the most widely consumed herbal plant in Asia and is well-known for its various pharmacological properties. Many studies have been devoted to this natural product. However, polysaccharide’s components of ginseng and their biological effects have not been widely studied. In this study, white ginseng neutral polysaccharide (WGNP) and white ginseng acidic polysaccharide (WGAP) fractions were purified from P. ginseng roots. The chemical properties of WGNP and WGAP were investigated using various chromatography and spectroscopy techniques, including high-performance gel permeation chromatography, Fourier-transform infrared spectroscopy, and high-performance liquid chromatography with an ultra-violet detector. The antioxidant, anti-radical, and hydrogen peroxide scavenging activities were evaluated in vitro and in vivo using Caenorhabditis elegans as the model organism. Our in vitro data by ABTS (2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid), reducing power, ferrous ion chelating, and hydroxyl radical scavenging activity suggested that the WGAP with significantly higher uronic acid content and higher molecular weight exhibits a much stronger antioxidant effect as compared to that of WGNP. Similar antioxidant activity of WGAP was also confirmed in vivo by evaluating internal reactive oxygen species (ROS) concentration and lipid peroxidation. In conclusion, WGAP may be used as a natural antioxidant with potent scavenging and metal chelation properties.

scholarly journals Antioxidant Activity of the Ethanol Extract of Manilkara zapota Leaf

2011 ◽  
Vol 4 (1) ◽  
pp. 193 ◽  
Author(s):  
M. E. Islam ◽  
M. S. Parvin ◽  
M. R. Islam ◽  
M. S. Islam ◽  
S. M. R. Hasan
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Serum Marker ◽  
Control Group ◽  
Marker Enzymes ◽  
Manilkara Zapota

The present study evaluated the antioxidant activity of cold ethanolic extract of Manilkara zapota (Sapotaceae) leaves. In vitro antioxidant activity was determined using 1, 1-diphenyl-2-picrylhydrazyl radical, reducing power capacity, total phenol and flavonoid content. The extract demonstrated significant dose dependent antioxidant activity in vitro methods. In DPPH radical scavenging assay IC50 values of Manilkara zapota leaves (MZL) and ascorbic acid (standard) were found to be 68.27 and 16.17 μg/ml, respectively. In vivo, the extract was evaluated by carbon tetrachloride (CCl4) induced liver damage rats in hepatoprotective model. CCl4 produced significant alteration of serum marker enzymes, total bilirubin, total protein and liver weight. Restoration of these values towards normal, which is comparable to control group, indicated hepatoprotective activity, which reflects the antioxidant potential of the extract. Results presented here indicate that MZL possess strong antioxidant activity and they can therefore be used as a good natural source of antioxidant.Keywords: MZL; DPPH; Scavenging activity; Serum marker enzymes.© 2012 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi: http://dx.doi.org/10.3329/jsr.v4i1.7148J. Sci. Res. 4 (1), 193-202 (2012)

scholarly journals Ethanolic Extract of Glycyrrhiza glabra to Ameliorate Oxidative Stress – Studies In vitro

2020 ◽  
Vol 10 (2) ◽  
pp. 2289-2297
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Glycyrrhiza Glabra ◽  
New Drugs ◽  
Phytochemical Analysis ◽  
Radical Scavenging Assay

Plants are major sources of bioactive organic molecules of interest for the pharmaceutical industry and are being screened for new drugs and chemicals. Glycyrrhiza glabra Linn. (Family: Fabaceae) is a very well-known medicinal plant traditionally used as medicine. Its roots and rhizomes are the medicinal parts used and are reported to possess antitumor, antimicrobial, antiviral, anti-inflammatory, immunoregulatory activities. The ethanolic extract of Glycyrrhiza glabra was prepared in the present work, and its phytochemical analysis and HPLC were done. The in vitro antioxidant activity assays such as DPPH radical scavenging assay, Hydroxyl radical scavenging assay, total antioxidant activity assay, and total reducing power assay was done, and the results showed significant antioxidant activity. The extract was analyzed further to evaluate the ability to protect against oxidative stress in chicken liver tissue. The extent of lipid peroxidation and glutathione in H2O2 and/or Glycyrrhiza glabra extract-treated tissue indicated the extract's potential to protect against oxidative stress under in vitro conditions. The in vitro comet assay results showed that the Glycyrrhiza glabra extract protected against H2O2 induced cellular DNA damage. These findings indicated promising antioxidant and antigenotoxic potential of Glycyrrhiza glabra and need further exploration for translating these findings to its possible health benefits.

Antioxidant Activity, Phenolic Content and Hematoprotective Effects of Cleome arabica Polyphenolic Leaf Extract

2019 ◽  
Author(s):  
C. Tigrine ◽  
A. Kameli
Keyword(s):  
Antioxidant Activity ◽  
Biological Effects ◽  
Reducing Power ◽  
Hematological Toxicity ◽  
Protective Effects ◽  
Ferrous Ions ◽  
Polyphenolic Extract ◽  
Cleome Arabica

In this study a polyphenolic extract from Cleome arabica leaves (CALE) was investigated for its antioxidant activity in vitro using DPPH•, metal chelating and reducing power methods and for its protective effects against AraC-induced hematological toxicity in vivo using Balb C mice. Results indicated that CALE exhibited a strong and dose-dependent scavenging activity against the DPPH• free radical (IC50 = 4.88 μg/ml) and a high reducing power activity (EC50 = 4.85 μg/ml). Furthermore, it showed a good chelating effects against ferrous ions (IC50 = 377.75 μg/ml). The analysis of blood showed that subcutaneous injection of AraC (50 mg/kg) to mice during three consecutive days caused a significant myelosupression (P < 0.05). The combination of CALE and AraC protected blood cells from a veritable toxicity. Where, the number of the red cells, the amount of hemoglobin and the percentage of the hematocrite were significantly high. On the other hand, AraC cause an elevation of body temperature (39 °C) in mice. However, the temperature of the group treated with CALE and AraC remained normal and did not exceed 37.5 °C. The observed biological effects of CALE, in vitro as well as in vivo, could be due to the high polyphenol and flavonoid contents. In addition, the antioxidant activity of CALE suggested to be responsible for its hematoprotective effect.

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