scholarly journals Polyketide-Derived Secondary Metabolites from a Dothideomycetes Fungus, Pseudopalawania siamensis gen. et sp. nov., (Muyocopronales) with Antimicrobial and Cytotoxic Activities

Biomolecules ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 569 ◽  
Author(s):  
Ausana Mapook ◽  
Allan Patrick G. Macabeo ◽  
Benjarong Thongbai ◽  
Kevin D. Hyde ◽  
Marc Stadler
Keyword(s):  
Mycobacterium Smegmatis ◽  
Fibroblast Cell ◽  
Culture Broth ◽  
Mouse Fibroblast ◽  
Cytotoxic Activities ◽  
Morphological Comparison ◽  
Cytotoxic Effects ◽  
Gram Positive Bacteria ◽  
Potent Inhibitory Activity ◽  
Fibroblast Cell Lines

Pseudopalawania siamensis gen. et sp. nov., from northern Thailand, is introduced based on multi-gene analyses and morphological comparison. An isolate was fermented in yeast malt culture broth and explored for its secondary metabolite production. Chromatographic purification of the crude ethyl acetate (broth) extract yielded four tetrahydroxanthones comprised of a new heterodimeric bistetrahydroxanthone, pseudopalawanone (1), two known dimeric derivatives, 4,4′-secalonic acid D (2) and penicillixanthone A (3), the corresponding monomeric tetrahydroxanthone paecilin B (4), and the known benzophenone, cephalanone F (5). Compounds 1–3 showed potent inhibitory activity against Gram-positive bacteria. Compounds 2 and 3 were inhibitory against Bacillus subtilis with minimum inhibitory concentrations (MIC) of 1.0 and 4.2 μg/mL, respectively. Only compound 2 showed activity against Mycobacterium smegmatis. In addition, the dimeric compounds 1–3 also showed moderate cytotoxic effects on HeLa and mouse fibroblast cell lines, which makes them less attractive as candidates for development of selectively acting antibiotics.

scholarly journals Extracellular vesicles released from irradiated neonatal mouse cheek tissue increased cell survival after radiation

2020 ◽  
Vol 62 (1) ◽  
pp. 73-78
Author(s):  
Kentaro Ariyoshi ◽  
Yota Hiroyama ◽  
Naoya Fujiwara ◽  
Tomisato Miura ◽  
Kosuke Kasai ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Fibroblast Cell ◽  
Mouse Fibroblast ◽  
High Dose ◽  
Protective Effects ◽  
X Ray ◽  
Secreted Factors ◽  
The Common ◽  
Dose Radiation ◽  
Fibroblast Cell Lines

Abstract Alopecia is one of the common symptoms after high-dose radiation exposure. In our experiments, neonatal mice that received 7 Gy X-ray exhibited defects in overall hair growth, except for their cheeks. This phenomenon might suggest that some substances were secreted and prevented hair follicle loss in the infant tissues around their cheeks after radiation damage. In this study, we focused on exosome-like vesicles (ELV) secreted from cheek skin tissues and back skin tissues, as control, and examined their radiation protective effects on mouse fibroblast cell lines. We observed that ELV from irradiated cheek skin showed protective effects from radiation. Our results suggest that ELV from radiation-exposed cheek skin tissue is one of the secreted factors that prevent hair follicle loss after high-dose radiation.

scholarly journals Antiproliferative Effects of a Series of Pyrazolines on Lung Cancer

Proceedings ◽  
2018 ◽  
Vol 2 (25) ◽  
pp. 1574
Author(s):  
Belgin Sever ◽  
Mehlika Dilek Altıntop ◽  
Elif Kaya Tilki ◽  
Miriş Dikmen ◽  
Ahmet Özdemir
Keyword(s):  
Lung Cancer ◽  
Anticancer Agents ◽  
Human Lung ◽  
Fibroblast Cell ◽  
Inhibitory Effect ◽  
Cytotoxic Effects ◽  
Human Lung Fibroblast ◽  
Antiproliferative Effects ◽  
Ic50 Value ◽  
Fibroblast Cell Lines

Lung cancer is one of the most diagnosed cancers worldwide and the development of anticancer agents for the treatment of lung cancer is an important task for researchers. For this purpose, herein pyrazoline-based compounds 1–18 were investigated for their cytotoxic effects on A549 human lung adenocarcinoma and CCD-19Lu human lung fibroblast cell lines using MTT assay. According to the results, 1-(4-methoxyphenyl)-3-(2-furanyl)-5-(4-methylphenyl)-2-pyrazoline (7) was identified as the most promising anticancer agent due to its selective inhibitory effect on A549 cell line with an IC50 value of 138.63 µg/mL when compared with cyclophosphamide (IC50 = 309.97 µg/mL).

scholarly journals Stimulation of Polo-Like Kinase 3 mRNA Decay by Tristetraprolin

2009 ◽  
Vol 29 (8) ◽  
pp. 1999-2010 ◽  
Author(s):  
Thierry J. Horner ◽  
Wi S. Lai ◽  
Deborah J. Stumpo ◽  
Perry J. Blackshear
Keyword(s):  
Protein Kinase ◽  
Cell Lines ◽  
Zinc Finger Protein ◽  
Fibroblast Cell ◽  
Mouse Fibroblast ◽  
Serum Stimulation ◽  
Ccch Zinc Finger ◽  
Finger Protein ◽  
Tandem Ccch Zinc Finger ◽  
Fibroblast Cell Lines

ABSTRACT Polo-like protein kinase 3 (Plk3) has been proposed to regulate entry into S phase and promote apoptosis in response to oxidative stress. Its mRNA contains three AU-rich elements (AREs) in its 3′ untranslated region (3′-UTR) that can contribute to the rapid degradation of labile transcripts. We investigated the possibility that tristetraprolin (TTP), a tandem CCCH zinc finger protein, could promote the decay of Plk3 transcripts. TTP is known to stimulate the deadenylation and decay of mRNAs possessing one or more copies of the consensus nonamer motif UUAUUUAUU. In stable mouse fibroblast cell lines derived from wild-type and TTP knockout littermates, the decay of Plk3 transcripts after serum stimulation was slowed in the absence of TTP. The specificity of TTP for promoting the degradation of Plk3 was demonstrated by the unaltered decay of Plk3 mRNA in cell lines deficient in the TTP family members ZFP36L1 and ZFP36L2. We also found that the AREs present in the Plk3 transcript were essential for both the binding of TTP to the 3′-UTR and promoting the destruction of target transcripts in cotransfection experiments. The regulation of Plk3 mRNA stability by TTP may influence the control of the cell cycle by this protein kinase.

scholarly journals Antimicrobial and cytotoxic activities of Barleria prionitis and Barleria grandiflora: A comparative study

2016 ◽  
Vol 11 (4) ◽  
pp. 802
Author(s):  
Hemant Arunrao Sawarkar ◽  
Pranita P. Kashyap ◽  
Ajit Kumar Pandey ◽  
Mukesh Kumar Singh ◽  
Chanchal Deep Kaur
Keyword(s):  
Dermal Fibroblast ◽  
Video Clip ◽  
Ethanolic Extract ◽  
Fibroblast Cell ◽  
Human Dermal Fibroblast ◽  
Human Gingival Fibroblast ◽  
Cytotoxic Activities ◽  
Gingival Fibroblast ◽  
Rural People ◽  
Fibroblast Cell Lines

<p class="Abstract">Leaf juices as well as leaves of <em>Barleria prionitis</em> and <em>Barleria grandiflora</em> are being used by rural people across various regions of India in the treatment of oral ailments such as dental troubles, gum ailments, pyorrhoea, dental carries and mouth ulcers. Zone of inhibition and MIC values obtained for all the extracts suggest ethanolic extract of the herbs were more antimicrobial when compared to the aqueous extract. Results of biofilm suppression were found statistically significant (p&lt;0.05) when compared to control. Cytotoxicity was evaluated by MTT assay on human gingival fibroblast and human dermal fibroblast cell lines for ethanolic extract of the herbs. CTC<sub>50</sub> value was found to be more than 1,000 µg/mL for ethanolic extracts of both herbs. Chlorhexidine was found to be more cytotoxic with CTC<sub>50 </sub>value of 12.5–25 µg/mL. Ethanolic extract of <em>B. prionitis</em> and <em>B. grandiflora</em> found significantly cytotoxic (p&lt;0.05) in comparison with control.</p><p class="Abstract"><strong>Video Clip:</strong></p><p class="Abstract"><a href="https://youtube.com/v/DNByJJQT3RY">Cytotoxicity assay:</a> 18 min 45 sec</p><p> </p>

Sign in / Sign up

Export Citation Format

Share Document