scholarly journals The Stimulatory Effect of Purine-Type Cytokinins on Proliferation and Polyphenolic Compound Accumulation in Shoot Culture of Salvia viridis

Biomolecules ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 178 ◽  
Author(s):  
Izabela Grzegorczyk-Karolak ◽  
Katarzyna Hnatuszko-Konka ◽  
Mariola Zarzycka ◽  
Łukasz Kuźma
Keyword(s):  
Therapeutic Potential ◽  
Shoot Culture ◽  
Total Content ◽  
Biomass Accumulation ◽  
Hormonal Control ◽  
Polyphenolic Compound ◽  
Physiological Processes ◽  
Best Response ◽  
Alternative Sources ◽  
Metabolite Content

The present study demonstrates hormonal control of Salvia viridis growth and development using four different purine-type cytokinins at different concentrations. The addition of cytokinins significantly increased biomass of cultures, proliferation rate, and, interestingly, secondary metabolite production. The best response in terms of multiplication ratio was recorded on Murashige and Skoog medium supplemented with 0.5 mg/L BPA (N-benzylotetrahydropyranyl adenine), while the greatest biomass accumulation was achieved when supplemented with 1 mg/L m-T (meta-topoline). Quantitative UPLC-DAD analysis of the hydromethanolic extract from S. viridis culture revealed the presence of 12 polyphenols: seven phenolic acids and five phenylethanoids. The highest total content of polyphenolic compounds was found in shoots cultivated on medium with 2 mg/L BPA (18.66 mg/g DW): almost twice that of control shoots. The medium was also the most optimal for the biosynthesis of rosmarinic acid, the predominant phenolic acid. However, the greater phenylethanoid accumulation was stimulated by 1 mg/L m-T: the metabolite content was above three times higher than that found in shoots grown on the control medium (8.03 mg/g DW vs. 2.37 mg/g DW). Hence, it was demonstrated that phytohormones are capable of influencing not only vital physiological processes, but therapeutic potential of plants as well. Therefore, the cytokinin-based sage cultures may be also considered as the alternative sources of bioactive compounds.

Therapeutic Potential of Agonists and Antagonists of A1, A2a, A2b and A3 Adenosine Receptors

2019 ◽  
Vol 25 (26) ◽  
pp. 2892-2905 ◽  
Author(s):  
Sumit Jamwal ◽  
Ashish Mittal ◽  
Puneet Kumar ◽  
Dana M. Alhayani ◽  
Amal Al-Aboudi
Keyword(s):  
Nervous System ◽  
Therapeutic Potential ◽  
The Body ◽  
Membrane Receptors ◽  
Physiological Importance ◽  
Physiological Processes ◽  
Central Nervous Systems ◽  
Energy Consuming ◽  
Metabolic Dysfunctions ◽  
G Protein Coupled

Adenosine is a naturally occurring nucleoside and an essential component of the energy production and utilization systems of the body. Adenosine is formed by the degradation of adenosine-triphosphate (ATP) during energy-consuming processes. Adenosine regulates numerous physiological processes through activation of four subtypes of G-protein coupled membrane receptors viz. A1, A2A, A2B and A3. Its physiological importance depends on the affinity of these receptors and the extracellular concentrations reached. ATP acts as a neurotransmitter in both peripheral and central nervous systems. In the peripheral nervous system, ATP is involved in chemical transmission in sensory and autonomic ganglia, whereas in central nervous system, ATP, released from synaptic terminals, induces fast excitatory postsynaptic currents. ATP provides the energetics for all muscle movements, heart beats, nerve signals and chemical reactions inside the body. Adenosine has been traditionally considered an inhibitor of neuronal activity and a regulator of cerebral blood flow. Since adenosine is neuroprotective against excitotoxic and metabolic dysfunctions observed in neurological and ocular diseases, the search for adenosinerelated drugs regulating adenosine transporters and receptors can be important for advancement of therapeutic strategies against these diseases. This review will summarize the therapeutic potential and recent SAR and pharmacology of adenosine and its receptor agonists and antagonists.

scholarly journals Biomass accumulation and physiological responses of tomato plants to magnetically–treated water in hydroponic conditions

2021 ◽  
Author(s):  
Daniel I. Ospina-Salazar ◽  
Shimon Rachmilevitch ◽  
Santiago Cuervo-Jurado ◽  
Orlando Zúñiga-Escobar
Keyword(s):  
Stomatal Conductance ◽  
Water Potential ◽  
Biomass Accumulation ◽  
Lower Surface ◽  
Tomato Plants ◽  
Treated Water ◽  
Fresh Biomass ◽  
Physiological Processes ◽  
Underlying Mechanisms ◽  
Hydroponic Conditions

AbstractMagnetically-treated water (MTW) has been reported to enhance biomass accumulation in plants. However, the underlying mechanisms are not fully understood, and the existing reports only deal with soil-grown plants. Thus, the purpose of this experiment was to assess whether or not MTW affects main physiological processes (gas exchange, biomass accumulation and water potential) in tomato plants whose water supply was only MTW. Two experiments were done in hydroponic semi-controlled conditions, consisting of a loop system with permanent recirculation of water through a non-uniform magnet. The plants grown under MTW showed a significant increase in chlorophyll content, photosynthesis and transpiration at high light irradiances, although the increase in stomatal conductance was less significant. MTW also increased fruit fresh biomass, number of fruits and root dry biomass in 61.7 %, 85.3 % and 30.3 % respectively, but this was only achieved at natural sunlight conditions. Moreover, treated plants showed higher root hydraulic conductance and leaf water potential, which is thought to be related with a lower surface tension of MTW, an effect that is consistent with previous studies. The higher biomass accumulation in tomato plants under MTW is likely explained because of a faster water transport from the roots to the leaves via xylem, which in turn increases H2O efflux and CO2 assimilation in the leaves, thanks to a higher stomatal conductance.

scholarly journals Yield, Characterization, and Possible Exploitation of Cannabis Sativa L. Roots Grown under Aeroponics Cultivation

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4889
Author(s):  
Fabio Ferrini ◽  
Daniele Fraternale ◽  
Sabrina Donati Donati Zeppa ◽  
Giancarlo Verardo ◽  
Andrea Gorassini ◽  
...  
Keyword(s):  
Cannabis Sativa ◽  
Therapeutic Potential ◽  
Total Content ◽  
Bioactive Molecules ◽  
Bioactive Metabolites ◽  
Cultivated Plants ◽  
Root Extracts ◽  
Cultivation Technique ◽  
Long Time ◽  
Health Promoting

Cannabis sativa L. has been used for a long time to obtain food, fiber, and as a medicinal and psychoactive plant. Today, the nutraceutical potential of C. sativa is being increasingly reappraised; however, C. sativa roots remain poorly studied, despite citations in the scientific literature. In this direction, we identified and quantified the presence of valuable bioactives (namely, β-sitosterol, stigmasterol, campesterol, friedelin, and epi-friedelanol) in the root extracts of C. sativa, a finding which might pave the way to the exploitation of the therapeutic potential of all parts of the C. sativa plant. To facilitate root harvesting and processing, aeroponic (AP) and aeroponic-elicited cultures (AEP) were established and compared to soil-cultivated plants (SP). Interestingly, considerably increased plant growth—particularly of the roots—and a significant increase (up to 20-fold in the case of β-sitosterol) in the total content of the aforementioned roots’ bioactive molecules were observed in AP and AEP. In conclusion, aeroponics, an easy, standardized, contaminant-free cultivation technique, facilitates the harvesting/processing of roots along with a greater production of their secondary bioactive metabolites, which could be utilized in the formulation of health-promoting and health-care products.

Moving Past Quinone-Methides: Recent Advances toward Minimizing Electrophilic Byproducts from COS/H2S Donors

2021 ◽  
Vol 21 ◽  
Author(s):  
Michael Pluth
Keyword(s):  
Hydrogen Sulfide ◽  
Carbonic Anhydrase ◽  
Therapeutic Potential ◽  
Carbonyl Sulfide ◽  
Quinone Methide ◽  
Quinone Methides ◽  
On Demand ◽  
Physiological Processes ◽  
Recent Approach ◽  
Alternative Approaches

: Hydrogen sulfide (H2S) is an important biomolecule that plays key signaling and protective roles in different physiological processes. With the goals of advancing both the available research tools and the associated therapeutic potential of H2S, researchers have developed different methods to deliver H2S on-demand in different biological contexts. A recent approach to develop such donors has been to design compounds that release carbonyl sulfide (COS), which is quickly converted to H2S in biological systems by the ubiquitous enzyme carbonic anhydrase (CA). Although highly diversifiable, many approaches using this general platform release quinone methides or related electrophiles after donor activation. Many such electrophiles are likely scavenged by water, but recent efforts have also expanded alternative approaches that minimize the formation of electrophilic byproducts generated after COS release. This mini-review focuses specifically on recent examples of COS-based H2S donors that do not generate quinone methide byproducts after donor activation.

scholarly journals Mammalian hibernation as a model of disuse osteoporosis: the effects of physical inactivity on bone metabolism, structure, and strength

2008 ◽  
Vol 295 (6) ◽  
pp. R1999-R2014 ◽  
Author(s):  
Meghan E. McGee-Lawrence ◽  
Hannah V. Carey ◽  
Seth W. Donahue
Keyword(s):  
Bone Loss ◽  
Bone Metabolism ◽  
Bone Structure ◽  
Weight Bearing ◽  
Hormonal Control ◽  
Ground Squirrels ◽  
Valuable Insight ◽  
Physiological Processes ◽  
Skeletal Loading ◽  
Insight Into

Reduced skeletal loading typically leads to bone loss because bone formation and bone resorption become unbalanced. Hibernation is a natural model of musculoskeletal disuse because hibernating animals greatly reduce weight-bearing activity, and therefore, they would be expected to lose bone. Some evidence suggests that small mammals like ground squirrels, bats, and hamsters do lose bone during hibernation, but the mechanism of bone loss is unclear. In contrast, hibernating bears maintain balanced bone remodeling and preserve bone structure and strength. Differences in the skeletal responses of bears and smaller mammals to hibernation may be due to differences in their hibernation patterns; smaller mammals may excrete calcium liberated from bone during periodic arousals throughout hibernation, leading to progressive bone loss over time, whereas bears may have evolved more sophisticated physiological processes to recycle calcium, prevent hypercalcemia, and maintain bone integrity. Investigating the roles of neural and hormonal control of bear bone metabolism could give valuable insight into translating the mechanisms that prevent disuse-induced bone loss in bears into novel therapies for treating osteoporosis.

scholarly journals Zirconia Nanoparticles Impact On Morphophysiological Data And Mineral Composition Of P. ostreatus

2015 ◽  
Vol 22 (2) ◽  
pp. 169-188
Author(s):  
Nelya V. Doroshkevich ◽  
Marina V. Frontasyeva ◽  
Viktor S. Doroshkevich ◽  
Olena S. Lygina ◽  
Artem V. Shylo ◽  
...  
Keyword(s):  
Physiological Activity ◽  
Culture Fluid ◽  
Biomass Accumulation ◽  
Weight Percent ◽  
Biological Tissues ◽  
Extracellular Proteins ◽  
Physiological Processes ◽  
Low Concentrations ◽  
Biomedical Technologies

Abstract Neutron activation analysis of the Pleurotus ostreatus showed that adding of solid solution of ZrO2-Y2O3 hydroxide and oxide (3 mol % Y2O3) nanoparticles of size 4 and 9 nm at a concentration of 0.2 weight percent in a nutrient medium (Czapek) alters the character of physiological processes in the biological tissues of the mushrooms. This is manifested in the form of a significant change in morphological and physiological characteristics of the mushrooms and the elemental composition of the dry biomass. In particular, it is shown that the intercalation of nanoparticles into the tissues of the mushrooms leads to an increase of 1.3-1.4 times (more than 2.6 g/dm3) of biomass accumulation (industrial strain HK 35) and decrease of 1.7-1.8 times (below 1.7-2.5 mg/mm3) of concentrations of extracellular proteins into the culture fluid at a substantially constant value of the acidity. It is shown that the addition of ZrO2+3 mol % Y2O3 nanoparticles of sizes 4 or 9 nm into tissue of mushroom at step of the mother mycelium in very small concentrations can alter effectively the chemical composition of the substances produced by the cells and consequently, its physiological activity. It is shown that the use of low concentrations of ZrO2 nanoparticles allow to increase the yield and resistance of crops to diseases up to 1.2-1.5 times, as well as in the long term can be used in biomedical technologies for the treatment of cancer diseases.

scholarly journals In vitro tissue culture and genetic analysis of two high-CBD medical cannabis (Cannabis sativa L.) breeding lines

Genetika ◽  
2020 ◽  
Vol 52 (3) ◽  
pp. 925-941 ◽  
Author(s):  
Spela Mestinsek-Mubi ◽  
Sinja Svetik ◽  
Marko Flajsman ◽  
Jana Murovec
Keyword(s):  
Tissue Culture ◽  
Cannabis Sativa ◽  
Therapeutic Potential ◽  
Culture Media ◽  
Shoot Culture ◽  
Medical Cannabis ◽  
Breeding Lines ◽  
In Vitro Tissue Culture ◽  
Tissue Culture Media

The species Cannabis sativa L. has recently witnessed a resurgence of interest all over the world due to its multipurpose applications and the scientific confirmation of its pharmacological properties. Genotypes with high cannabinoid content are appreciated in the pharmaceutical and cosmetic industries due to their therapeutic potential. These genotypes, with predominantly high cannabidiol (CBD) content, are the subject of research and breeding in several programs, but to date, little data is published on the in vitro tissue culture of cannabis. Our study focused on the establishment of an efficient micropropagation method for two high-CBD breeding lines (MX-CBD-11 and MX-CBD-707) as the basis for advanced biotechnological breeding approaches. The results demonstrated that the in vitro culture of medical cannabis can be initiated on different culture media, that cultured plants can be successfully acclimatized, and that nodal position, and especially the genotype, have a significant influence on the success of shoot culture establishment. They showed that the published tissue culture media optimized for one high-THC strain of Mexican cannabis are not as efficient for other genotypes of (medical) cannabis. We complemented this research with a genetic study of 95 plants of the two breeding lines with 16 microsatellite (SSR) markers which clustered the plants based on breeding line. The results demonstrated that only 8 markers are needed for discrimination of all analyzed plants and their usefulness for clonal identification.

scholarly journals Circular RNAs and Hepatocellular Carcinoma: New Epigenetic Players With Diagnostic and Prognostic Roles

2021 ◽  
Vol 11 ◽  
Author(s):  
Kedeerya Aishanjiang ◽  
Xin-dong Wei ◽  
Yi Fu ◽  
Xinjie Lin ◽  
Yujie Ma ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Hepatocellular Carcinoma ◽  
Closed Loop ◽  
Therapeutic Potential ◽  
Therapeutic Targets ◽  
Circular Rnas ◽  
Diagnosis And Prognosis ◽  
Physiological Processes ◽  
Non Coding Rnas ◽  
Novel Targets

Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related death worldwide. Due to the lack of potent diagnosis and prognosis biomarkers and effective therapeutic targets, the overall prognosis of survival is poor in HCC patients. Circular RNAs (circRNAs) are a class of novel endogenous non-coding RNAs with covalently closed loop structures and implicated in diverse physiological processes and pathological diseases. Recent studies have demonstrated the involvement of circRNAs in HCC diagnosis, prognosis, development, and drug resistance, suggesting that circRNAs may be a class of novel targets for improving HCC diagnosis, prognosis, and treatments. In fact, some artificial circRNAs have been engineered and showed their therapeutic potential in treating HCV infection and gastric cancer. In this review, we introduce the potential of circRNAs as biomarkers for HCC diagnosis and prognosis, as therapeutic targets for HCC treatments and discuss the challenges in circRNA research and chances of circRNA application.

scholarly journals 502 Intermittent CO2 Enrichment for Hydroponic Lettuce Production

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 532A-532 ◽  
Author(s):  
David S. de Villiers ◽  
Robert W. Langhans ◽  
A.J. Both ◽  
Louis D. Albright ◽  
Sue Sue Scholl
Keyword(s):  
Gas Exchange ◽  
Carbon Assimilation ◽  
Co2 Enrichment ◽  
Biomass Accumulation ◽  
Light Level ◽  
Daily Light Integral ◽  
Physiological Processes ◽  
Light Levels ◽  
Growth Increments ◽  
Supplemental Lighting

CO2 enrichment increases efficiency of light utilization and rate of growth, thereby reducing the need for supplemental lighting and potentially lowering cost of production. However, during warmer periods of the year, CO2 enrichment is only possible intermittently due to the need to vent for temperature control. Previous research investigated the separate and combined effects of daily light integral and continuous CO2 enrichment on biomass accumulation in lettuce. The current research was designed to look at the efficiency with which lettuce is able to utilize intermittent CO2 enrichment, test the accuracy with which growth can be predicted and controlled, and examine effects of varying CO2 enrichment and supplemental lighting on carbon assimilation and plant transpiration on a minute by minute basis. Experiments included application of various schedules of intermittent CO2 enrichment and gas exchange analysis to elucidate underlying physiological processes. Same-day and day-to-day adjustments in daily light integrals were made in response to occasional CO2 venting episodes, using an up-to-the-minute estimate of growth progress based on an integration of growth increments that were calculated from actual light levels and CO2 concentrations experienced by the plants. Results indicated lettuce integrates periods of intermittent CO2 enrichment well, achieving expected growth targets as measured by destructive sampling. The gas-exchange work quantified a pervasive impact of instantaneous light level and CO2 concentration on conductance and CO2 assimilation. Implications for when to apply supplemental lighting and CO2 enrichment to best advantage and methods for predicting and controlling growth under intermittent CO2 enrichment are discussed.

scholarly journals Respons fisiologi pertumbuhan dan hasil tiga genotip jawawut terhadap cekaman kekeringan

Jurnal Agro ◽  
10.15575/4590 ◽  
2019 ◽  
Vol 6 (1) ◽  
pp. 35-48
Author(s):  
Yuyun Yuwariah ◽  
Sheli Mustikasari Dewi ◽  
Warid Ali Qosim ◽  
Anne Nuraini
Keyword(s):  
Field Capacity ◽  
Growth And Yield ◽  
Food Sources ◽  
Experimental Station ◽  
Physiological Processes ◽  
Best Response ◽  
Split Plot ◽  
Main Plot ◽  
Three Stages ◽  
Plot Design

Jawawut merupakan salah satu tanaman pangan lokal Indonesia yang belum banyak dikembangkan dan dimanfaatkan sebagai sumber pangan. Penelitian ini bertujuan untuk mendapatkan genotip jawawut yang memberikan pengaruh paling baik terhadap pertumbuhan dan hasil pada berbagai tingkat pemberian air di rumah plastik. Penelitian dilaksanakan pada bulan Juni sampai dengan September 2017 di Kebun Percobaan Fakultas Pertanian, Universitas Padjadjaran. Penelitian menggunakan Rancangan Petak Terbagi (Split Plot Design) dengan 3 ulangan. Petak utama terdiri dari tiga macam genotip yaitu genotip 44, 46, dan 48. Anak petak terdiri dari tiga taraf kapasitas lapang  yaitu 75%, 50% dan 25%. Hasil penelitian menunjukkan perlakuan pemberian air 25% kapasitas lapang berpengaruh paling buruk terhadap  proses fisiologis pertumbuhan dan hasil tiga genotip jawawut. Genotip 44 dan 46, pada pemberian air 50 % KL menghasilkan  konduktan stomata terbaik. Genotip 46 dan 48 memberikan pengaruh paling baik terhadap proses fisiologis pertumbuhan dan hasil jawawut yaitu jumlah anakan per rumpun.ABSTRACTMillet is one of Indonesia's local food crops that has not been widely developed as food sources. The purpose of this study was to obtain the genotypes of millet which gave the best effect on the growth and yield at various levels of water supply in the plastic house. The study was conducted from June to September 2017 at the Experimental Station of the Faculty of Agriculture, Universitas Padjadjaran. The research used the Split Plot Design with three replications. The main plot factor consisted of three levels of treatment; genotypes 44, 46, and 48. The subplot factor consisted of three stages of different treatment of the field capacity; 75%, 50% and 25%. The results showed the treatment of 25% water to field capacity had the worst effect on the physiological process of growth and yield of three millet genotypes. Genotypes 44 and 46, at 50% field capacity, produced the best stomatal conductance. Genotype 46 and 48 showed the best response to the physiological processes for the number of tillers.

Sign in / Sign up

Export Citation Format

Share Document