scholarly journals High-Resolution Imaging for the Analysis and Reconstruction of 3D Microenvironments for Regenerative Medicine: An Application-Focused Review

2021 ◽  
Vol 8 (11) ◽  
pp. 182
Author(s):  
Michail E. Klontzas ◽  
Alexandros Protonotarios
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Multiphoton Microscopy ◽  
Rapid Evolution ◽  
Matrix Composition ◽  
Great Promise ◽  
Imaging Methods ◽  
Scaffold Structure ◽  
Magnetic Resonance Imaging Mri ◽  
Cellular Behaviour

The rapid evolution of regenerative medicine and its associated scientific fields, such as tissue engineering, has provided great promise for multiple applications where replacement and regeneration of damaged or lost tissue is required. In order to evaluate and optimise the tissue engineering techniques, visualisation of the material of interest is crucial. This includes monitoring of the cellular behaviour, extracellular matrix composition, scaffold structure, and other crucial elements of biomaterials. Non-invasive visualisation of artificial tissues is important at all stages of development and clinical translation. A variety of preclinical and clinical imaging methods—including confocal multiphoton microscopy, optical coherence tomography, magnetic resonance imaging (MRI), and computed tomography (CT)—have been used for the evaluation of artificial tissues. This review attempts to present the imaging methods available to assess the composition and quality of 3D microenvironments, as well as their integration with human tissues once implanted in the human body. The review provides tissue-specific application examples to demonstrate the applicability of such methods on cardiovascular, musculoskeletal, and neural tissue engineering.

scholarly journals Cell Microenvironment Engineering and Monitoring for Tissue Engineering and Regenerative Medicine: The Recent Advances

2014 ◽  
Vol 2014 ◽  
pp. 1-18 ◽  
Author(s):  
Julien Barthes ◽  
Hayriye Özçelik ◽  
Mathilde Hindié ◽  
Albana Ndreu-Halili ◽  
Anwarul Hasan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Control Cell ◽  
Matrix Composition ◽  
Tissue Engineering Scaffolds ◽  
Cell Microenvironment ◽  
Engineered Tissues ◽  
Cellular Behaviour

In tissue engineering and regenerative medicine, the conditions in the immediate vicinity of the cells have a direct effect on cells’ behaviour and subsequently on clinical outcomes. Physical, chemical, and biological control of cell microenvironment are of crucial importance for the ability to direct and control cell behaviour in 3-dimensional tissue engineering scaffolds spatially and temporally. In this review, we will focus on the different aspects of cell microenvironment such as surface micro-, nanotopography, extracellular matrix composition and distribution, controlled release of soluble factors, and mechanical stress/strain conditions and how these aspects and their interactions can be used to achieve a higher degree of control over cellular activities. The effect of these parameters on the cellular behaviour within tissue engineering context is discussed and how these parameters are used to develop engineered tissues is elaborated. Also, recent techniques developed for the monitoring of the cell microenvironmentin vitroandin vivoare reviewed, together with recent tissue engineering applications where the control of cell microenvironment has been exploited. Cell microenvironment engineering and monitoring are crucial parts of tissue engineering efforts and systems which utilize different components of the cell microenvironment simultaneously can provide more functional engineered tissues in the near future.

scholarly journals A Precised Surface Modification of Hydroxyapatite with Poly(methylmethacrylate) for Tissue Engineering & Regenerative Medicine

2019 ◽  
Vol 31 (3) ◽  
pp. 545-550
Author(s):  
Trinh Duy Nguyen ◽  
Phu Thuong Nhan Nguyen ◽  
Thien Hien Tran ◽  
Md. Rafiqul Islam ◽  
Kwon Taek Lim ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Radical Polymerization ◽  
Colloidal Stability ◽  
Hybrid Nanocomposites ◽  
Great Promise ◽  
Dsc Studies ◽  
One Step ◽  
Grafting From ◽  
Hydroxyapatite Nanocrystals

The poly(methylmethacrylate) (PMMA) grafted biocompatible hydroxyapatite nanocrystals (HAPs) hybrid nanocomposites (PMMA-g-HAPs) were synthesized by employing surface thiol-lactam initiated radical polymerization (TLIRP) through grafting from strategy. At first, the surface of HAPs was functionalized by 3-mercaptopropyl-trimethoxysilane in one-step process to prepare thiol immobilized HAPs (HAPs-SH). Subsequently, a controlled radical polymerization of MMA by using two component initiating system comprising of HAPs-SH and butyrolactam (BL) successfully afforded PMMA-g-HAPs nanocomposites. The resulting structure and morphological feature of nanocomposites was systematically characterized by FT-IR and XRD analyses. GPC studies of cleaved polymers from nanocomposites of different time revealed that the grafting polymerization from the surface of HAP was well controlled in nature. Moreover, the thermal property of the PMMA was found to be improved by incorporation of inorganic HAP nanoparticles in the polymer matrix as revealed by TGA and DSC studies. The colloidal stability of the synthesized nanocomposites was observed to be exceptionally good in organic solvents as suggested by the time dependent monitoring using UV-visible spectroscopy and captured digital photographs. The synthesized nanocomposites show a great promise for the safe application in tissue engineering and regenerative medicine.

scholarly journals Advances in Regenerative Medicine and Tissue Engineering: Innovation and Transformation of Medicine

2018 ◽  
Vol 2018 ◽  
pp. 1-24 ◽  
Author(s):  
Kevin Dzobo ◽  
Nicholas Ekow Thomford ◽  
Dimakatso Alice Senthebane ◽  
Hendrina Shipanga ◽  
Arielle Rowe ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Three Dimensional ◽  
The Body ◽  
Congenital Defects ◽  
Great Promise ◽  
Disruptive Technology ◽  
3D Bioprinting ◽  
Huge Impact ◽  
Made In

Humans and animals lose tissues and organs due to congenital defects, trauma, and diseases. The human body has a low regenerative potential as opposed to the urodele amphibians commonly referred to as salamanders. Globally, millions of people would benefit immensely if tissues and organs can be replaced on demand. Traditionally, transplantation of intact tissues and organs has been the bedrock to replace damaged and diseased parts of the body. The sole reliance on transplantation has created a waiting list of people requiring donated tissues and organs, and generally, supply cannot meet the demand. The total cost to society in terms of caring for patients with failing organs and debilitating diseases is enormous. Scientists and clinicians, motivated by the need to develop safe and reliable sources of tissues and organs, have been improving therapies and technologies that can regenerate tissues and in some cases create new tissues altogether. Tissue engineering and/or regenerative medicine are fields of life science employing both engineering and biological principles to create new tissues and organs and to promote the regeneration of damaged or diseased tissues and organs. Major advances and innovations are being made in the fields of tissue engineering and regenerative medicine and have a huge impact on three-dimensional bioprinting (3D bioprinting) of tissues and organs. 3D bioprinting holds great promise for artificial tissue and organ bioprinting, thereby revolutionizing the field of regenerative medicine. This review discusses how recent advances in the field of regenerative medicine and tissue engineering can improve 3D bioprinting and vice versa. Several challenges must be overcome in the application of 3D bioprinting before this disruptive technology is widely used to create organotypic constructs for regenerative medicine.

Isomeric control of the mechanical properties of supramolecular filament hydrogels

2018 ◽  
Vol 6 (1) ◽  
pp. 216-224 ◽  
Author(s):  
Yi-An Lin ◽  
Myungshim Kang ◽  
Wei-Chiang Chen ◽  
Yu-Chuan Ou ◽  
Andrew G. Cheetham ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Drug Delivery ◽  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Fine Tuning ◽  
Great Promise ◽  
Network Structures ◽  
Peptide Design

Supramolecular filament hydrogels are an emerging class of biomaterials that hold great promise for regenerative medicine, tissue engineering, and drug delivery. The use of isomeric hydrocarbons in the peptide design enables fine-tuning of the mechanical properties of their supramolecular filament hydrogels without altering their network structures.

scholarly journals Biofabrication offers future hope for tackling various obstacles and challenges in tissue engineering and regenerative medicine: A Prospective

2018 ◽  
Vol 5 (1) ◽  
Author(s):  
Tanveer Ahmad Mir ◽  
Shintaroh Iwanaga ◽  
Taketoshi Kurooka ◽  
Hideki Toda ◽  
Shinji Sakai ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Regenerative Medicine ◽  
Clinical Medicine ◽  
Three Dimensional ◽  
Point Of View ◽  
Great Promise ◽  
Organ Shortage ◽  
Innovation Research ◽  
Organ Specific

Biofabrication is an emerging multidisciplinary field that makes a revolutionary impact on the researches on life science, biomedical engineering, and both basic and clinical medicine, has progressed tremendously over the past few years. Recently, there has been a big boom in three-dimensional (3D) printing or additive manufacturing (AM) research worldwide, and there is a significant increase not only in the number of researchers turning their attention to AM but also publications demonstrating the potential applications of 3D printing techniques in multiple fields. Biofabrication and bioprinting hold great promise for the innovation of engineering-based organ replacing medicine. In this mini review, various challenges in the field of tissue engineering are focused from the point of view of the biofabrication - strategies to bridge the gap between organ shortage and mission of medical innovation research seek to achieve organ-specific treatments or regenerative therapies. Four major challenges are discussed including (i) challenge of producing organs by AM, (ii) digitalization of tissue engineering and regenerative medicine, (iii) rapid production of organs beyond the biological natural course, and (iv) extracorporeal organ engineering.

scholarly journals Taking a deep look: modern microscopy technologies to optimize the design and functionality of biocompatible scaffolds for tissue engineering in regenerative medicine

2013 ◽  
Vol 10 (86) ◽  
pp. 20130263 ◽  
Author(s):  
M. Vielreicher ◽  
S. Schürmann ◽  
R. Detsch ◽  
M. A. Schmidt ◽  
A. Buttgereit ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Harmonic Generation ◽  
Cultured Cells ◽  
Second Harmonic ◽  
Multiphoton Microscopy ◽  
Cell Encapsulation ◽  
Conventional Microscopy ◽  
Indispensable Tool ◽  
Anti Stokes

This review focuses on modern nonlinear optical microscopy (NLOM) methods that are increasingly being used in the field of tissue engineering (TE) to image tissue non-invasively and without labelling in depths unreached by conventional microscopy techniques. With NLOM techniques, biomaterial matrices, cultured cells and their produced extracellular matrix may be visualized with high resolution. After introducing classical imaging methodologies such as µCT, MRI, optical coherence tomography, electron microscopy and conventional microscopy two-photon fluorescence (2-PF) and second harmonic generation (SHG) imaging are described in detail (principle, power, limitations) together with their most widely used TE applications. Besides our own cell encapsulation, cell printing and collagen scaffolding systems and their NLOM imaging the most current research articles will be reviewed. These cover imaging of autofluorescence and fluorescence-labelled tissue and biomaterial structures, SHG-based quantitative morphometry of collagen I and other proteins, imaging of vascularization and online monitoring techniques in TE. Finally, some insight is given into state-of-the-art three-photon-based imaging methods (e.g. coherent anti-Stokes Raman scattering, third harmonic generation). This review provides an overview of the powerful and constantly evolving field of multiphoton microscopy, which is a powerful and indispensable tool for the development of artificial tissues in regenerative medicine and which is likely to gain importance also as a means for general diagnostic medical imaging.

Tissue engineering in regenerative medicine

2015 ◽  
Vol 6 (5) ◽  
pp. 291-298
Author(s):  
Barbara Różalska ◽  
Bartłomiej Micota ◽  
Małgorzata Paszkiewicz ◽  
Beata Sadowska
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine

A Photoactivatable α5β1-Specific Integrin Ligand

2018 ◽  
Author(s):  
Roshna Vakkeel ◽  
Aleeza Farrukh ◽  
Aranzazu del Campo
Keyword(s):  
Cellular Response ◽  
Light Exposure ◽  
Matrix Composition ◽  
Cellular Behavior ◽  
Dynamic Variations ◽  
In Situ Activation ◽  
Controlled Exposure ◽  
Cellular Behaviour ◽  
Integrin Ligand

In order to study how dynamic changes of α5β1 integrin engagement affect cellular behaviour, photoactivatable derivatives of α5β1 specific ligands are presented in this article. The presence of the photoremovable protecting group (PRPG) introduced at a relevant position for integrin recognition, temporally inhibits ligand bioactivity. Light exposure at cell-compatible dose efficiently cleaves the PRPG and restores functionality. Selective cell response (attachment, spreading, migration) to the activated ligand on the surface is achieved upon controlled exposure. Spatial and temporal control of the cellular response is demonstrated, including the possibility to in situ activation. Photoactivatable integrin-selective ligands in model microenvironments will allow the study of cellular behavior in response to changes in the activation of individual integrins as consequence of dynamic variations of matrix composition.

Graphene and Graphene-Based Materials in Biomedical Applications

2019 ◽  
Vol 26 (38) ◽  
pp. 6834-6850 ◽  
Author(s):  
Mohammad Omaish Ansari ◽  
Kalamegam Gauthaman ◽  
Abdurahman Essa ◽  
Sidi A. Bencherif ◽  
Adnan Memic
Keyword(s):  
Tissue Engineering ◽  
Thermal Properties ◽  
Gene Delivery ◽  
Regenerative Medicine ◽  
Biomedical Research ◽  
Biomedical Applications ◽  
Biomedical Application ◽  
Two Dimensional ◽  
Drug And Gene Delivery ◽  
Biomedical Fields

: Nanobiotechnology has huge potential in the field of regenerative medicine. One of the main drivers has been the development of novel nanomaterials. One developing class of materials is graphene and its derivatives recognized for their novel properties present on the nanoscale. In particular, graphene and graphene-based nanomaterials have been shown to have excellent electrical, mechanical, optical and thermal properties. Due to these unique properties coupled with the ability to tune their biocompatibility, these nanomaterials have been propelled for various applications. Most recently, these two-dimensional nanomaterials have been widely recognized for their utility in biomedical research. In this review, a brief overview of the strategies to synthesize graphene and its derivatives are discussed. Next, the biocompatibility profile of these nanomaterials as a precursor to their biomedical application is reviewed. Finally, recent applications of graphene-based nanomaterials in various biomedical fields including tissue engineering, drug and gene delivery, biosensing and bioimaging as well as other biorelated studies are highlighted.

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