scholarly journals Genitourinary Tissue Engineering: Reconstruction and Research Models

2021 ◽  
Vol 8 (7) ◽  
pp. 99
Author(s):  
Christophe Caneparo ◽  
David Brownell ◽  
Stéphane Chabaud ◽  
Stéphane Bolduc
Keyword(s):  
Tissue Engineering ◽  
Self Assembly ◽  
Treatment Options ◽  
Culture Conditions ◽  
Translation Rate ◽  
Adequate Treatment ◽  
Plastic Surfaces ◽  
Cell Culture Conditions ◽  
Tissue Production ◽  
Specific Organisation

Tissue engineering is an emerging field of research that initially aimed to produce 3D tissues to bypass the lack of adequate tissues for the repair or replacement of deficient organs. The basis of tissue engineering protocols is to create scaffolds, which can have a synthetic or natural origin, seeded or not with cells. At the same time, more and more studies have indicated the low clinic translation rate of research realised using standard cell culture conditions, i.e., cells on plastic surfaces or using animal models that are too different from humans. New models are needed to mimic the 3D organisation of tissue and the cells themselves and the interaction between cells and the extracellular matrix. In this regard, urology and gynaecology fields are of particular interest. The urethra and vagina can be sites suffering from many pathologies without currently adequate treatment options. Due to the specific organisation of the human urethral/bladder and vaginal epithelium, current research models remain poorly representative. In this review, the anatomy, the current pathologies, and the treatments will be described before focusing on producing tissues and research models using tissue engineering. An emphasis is made on the self-assembly approach, which allows tissue production without the need for biomaterials.

scholarly journals 3D Hepatic Organoid-Based Advancements in LIVER Tissue Engineering

2021 ◽  
Vol 8 (11) ◽  
pp. 185
Author(s):  
Amit Panwar ◽  
Prativa Das ◽  
Lay Poh Tan
Keyword(s):  
Tissue Engineering ◽  
Self Assembly ◽  
Liver Tissue ◽  
3D Culture ◽  
Culture Method ◽  
Culture Conditions ◽  
Phenotypic Properties ◽  
Liver Tissue Engineering

Liver-associated diseases and tissue engineering approaches based on in vitro culture of functional Primary human hepatocytes (PHH) had been restricted by the rapid de-differentiation in 2D culture conditions which restricted their usability. It was proven that cells growing in 3D format can better mimic the in vivo microenvironment, and thus help in maintaining metabolic activity, phenotypic properties, and longevity of the in vitro cultures. Again, the culture method and type of cell population are also recognized as important parameters for functional maintenance of primary hepatocytes. Hepatic organoids formed by self-assembly of hepatic cells are microtissues, and were able to show long-term in vitro maintenance of hepato-specific characteristics. Thus, hepatic organoids were recognized as an effective tool for screening potential cures and modeling liver diseases effectively. The current review summarizes the importance of 3D hepatic organoid culture over other conventional 2D and 3D culture models and its applicability in Liver tissue engineering.

Laser microstructured scaffolds for tissue engineering under dynamic cell culture conditions

2020 ◽  
Author(s):  
Ελευθερία Μπαμπαλιάρη
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Culture Conditions ◽  
Dynamic Cell ◽  
Cell Culture Conditions

Παρόλο που το περιφερικό νευρικό σύστημα εμφανίζει υψηλότερο ρυθμό αναγέννησης από εκείνο του κεντρικού νευρικού συστήματος μέσω αυθόρμητης αναγέννησης μετά από έναν τραυματισμό, η καθοδηγούμενη αξονική νευρική αναγέννηση και η λειτουργική αποκατάσταση είναι αρκετά σπάνια. Συνεπώς, η ανάπτυξη επιτυχημένων μεθόδων για την καθοδήγηση της νευρικής ανάπτυξης, «in vitro», είναι υψίστης σημασίας. Έχει αναφερθεί λεπτομερώς ότι η τοπογραφία του υποστρώματος επηρεάζει την ανάπτυξη, τον προσανατολισμό και τη διαφοροποίηση των νευρικών κυττάρων. Ωστόσο, η συνδυασμένη δράση της διατμητικής τάσης και της τοπογραφίας του υποστρώματος στην νευρική ανάπτυξη έχει ελάχιστα μελετηθεί, παρόλο που οι διατμητικές τάσεις είναι ευρέως γνωστό ότι διαδραματίζουν καθοριστικό ρόλο στην οργάνωση, ανάπτυξη και λειτουργία των ιστών. Σε αυτή τη μελέτη, ένα σύστημα μικροροών ακριβούς ελεγχόμενης ροής με συγκεκριμένους ειδικά σχεδιασμένους θαλάμους, που ενσωματώνουν μικροδομημένα υποστρώματα λέιζερ, αναπτύχθηκε για να μελετηθεί η συνδυασμένη δράση της διατμητικής τάσης και της τοπογραφίας υποστρώματος στην ανάπτυξη, στον προσανατολισμό, στην επιμήκυνση και στη διαφοροποίηση νευρικών κυττάρων. Πολυμερικά μικροδομημένα υποστρώματα, με ελεγχόμενη γεωμετρία και κανονικότητα μοτίβου, κατασκευάστηκαν με χρήση υπερβραχέων παλμών λέιζερ. Πραγματοποιήθηκε συγκριτική μελέτη μεταξύ στατικών και δυναμικών κυτταρικών καλλιεργειών για να αξιολογηθεί η συνεργατική ή ανταγωνιστική επίδραση της διατμητικής τάσης και της τοπογραφίας στη συμπεριφορά των νευρικών κυττάρων. Τα αποτελέσματα της κυτταρικής καλλιέργειας συμπληρώθηκαν με υπολογιστικές προσομοιώσεις ροής με σκοπό τον ακριβή υπολογισμό των αντίστοιχων τιμών διατμητικής τάσης.

scholarly journals Differential Behavior Between Isolated and Aggregated Rabbit Auricular Chondrocytes on Plastic Surfaces

2004 ◽  
Vol 2004 (2) ◽  
pp. 86-92 ◽  
Author(s):  
V. Villar-Suárez ◽  
I. Calles-Venal ◽  
I. G. Bravo ◽  
J. G. Fernández-Álvarez ◽  
M. Fernández-Caso ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Density ◽  
Morphological Differentiation ◽  
Type Ii Collagen ◽  
Culture Conditions ◽  
Type Ii ◽  
Isolation Method ◽  
Simple Method ◽  
Differential Behavior ◽  
Plastic Surfaces

A knowledge of the behavior of chondrocytes in culture is relevant for tissue engineering. Chondrocytes dedifferentiate to a fibroblast-like phenotype on plastic surfaces. Dedifferentiation is reversible if these cells are then cultured in suspension. In this report a description is given of how when chondrocyte aggregates formed in suspension are next seeded on plastic, most of them attach as round or polygonal cells. This morphological differentiation, with synthesis of type II collagen, is stable for long culture periods. This simple method can be of use as a model for studies of chondrocyte behavior on plastic. The results indicate that in addition to culture conditions, such as cell isolation method or cell density, chondrocyte behavior on plastic depends on the presence of aggregates.

Lectin Binding Studies on RNA Tumor Virus-Infected Cells

1975 ◽  
Vol 33 ◽  
pp. 326-327
Author(s):  
D. C. Hixson
Keyword(s):  
Binding Sites ◽  
Lectin Binding ◽  
Culture Conditions ◽  
3T3 Cells ◽  
Binding Studies ◽  
Con A ◽  
Microscopic Studies ◽  
Tumor Virus ◽  
Infected Cells ◽  
Cell Culture Conditions

The abilities of plant lectins to preferentially agglutinate malignant cells and to bind to specific monosaccharide or oligosaccharide sequences of glycoproteins and glycolipids make them a new and important biochemical probe for investigating alterations in plasma membrane structure which may result from malignant transformation. Electron and light microscopic studies have demonstrated clustered binding sites on surfaces of SV40-infected or tryp- sinized 3T3 cells when labeled with concanavalin A (con A). No clustering of con A binding sites was observed in normal 3T3 cells. It has been proposed that topological rearrangement of lectin binding sites into clusters enables con A to agglutinate SV40-infected or trypsinized 3T3 cells (1). However, observations by other investigators have not been consistent with this proposal (2) perhaps due to differences in reagents used, cell culture conditions, or labeling techniques. The present work was undertaken to study the lectin binding properties of normal and RNA tumor virus-infected cells and their associated viruses using lectins and ferritin-conjugated lectins of five different specificities.

scholarly journals The effects of cononsolvents on the synthesis of responsive particles via polymerisation-induced thermal self-assembly

2021 ◽  
Author(s):  
Marissa Morales-Moctezuma ◽  
Sebastian G Spain
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Self Assembly ◽  
Biomedical Applications

Nanogels have emerged as innovative platforms for numerous biomedical applications including gene and drug delivery, biosensors, imaging, and tissue engineering. Polymerisation-induced thermal self-assembly (PITSA) has been shown to be suitable...

scholarly journals Electrochemical Stability of Poly(3,4-Ethylenedioxythiophene) Derivatives Under Cell Culture Conditions

2021 ◽  
Vol 1885 (3) ◽  
pp. 032004
Author(s):  
Qichao Pan ◽  
Zuwei Zhang ◽  
Yaqiong Zhang ◽  
Yaopeng Zhang ◽  
Bo Zhu
Keyword(s):  
Cell Culture ◽  
Culture Conditions ◽  
Electrochemical Stability ◽  
Cell Culture Conditions

scholarly journals Review: The Self-Assembly Approach for Organ Reconstruction by Tissue Engineering

2000 ◽  
Vol 1 (5) ◽  
pp. 75-86 ◽  
Author(s):  
F.A. Auger ◽  
M. Rémy-Zolghadri ◽  
G. Grenier ◽  
L. Germain
Keyword(s):  
Tissue Engineering ◽  
Self Assembly ◽  
The Self

Influence of cell culture conditions on aromatase activity in human genital skin fibroblasts

1989 ◽  
Vol 25 (9) ◽  
pp. 806-812 ◽  
Author(s):  
Tarek Bisat ◽  
Terry R. Brown ◽  
Claude J. Migeon ◽  
Gary D. Berkovitz
Keyword(s):  
Cell Culture ◽  
Culture Conditions ◽  
Skin Fibroblasts ◽  
Aromatase Activity ◽  
Cell Culture Conditions
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