scholarly journals Strategy for Managing Industrial Anaerobic Sludge through the Heterotrophic Cultivation of Chlorella sorokiniana: Effect of Iron Addition on Biomass and Lipid Production

2021 ◽  
Vol 8 (6) ◽  
pp. 82
Author(s):  
Esteban Charria-Girón ◽  
Vanessa Amazo ◽  
Daniela De Angulo ◽  
Eliana Hidalgo ◽  
María Francisca Villegas-Torres ◽  
...  
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Specific Growth ◽  
Lipid Production ◽  
Oxygen Transfer Rate ◽  
Treatment Plant ◽  
Acid Synthesis ◽  
Chlorella Sorokiniana ◽  
Anaerobic Sludge ◽  
Heterotrophic Cultivation

Microalgae provides an alternative for the valorization of industrial by-products, in which the nutritional content varies substantially and directly affects microalgae system performance. Herein, the heterotrophic cultivation of Chlorella sorokiniana was systematically studied, allowing us to detect a nutritional deficiency other than the carbon source through assessing the oxygen transfer rate for glucose or acetate fermentation. Consequently, a mathematical model of the iron co-limiting effect on heterotrophic microalgae was developed by exploring its ability to regulate the specific growth rate and yield. For instance, higher values of the specific growth rate (0.17 h−1) compared with those reported for the heterotrophic culture of Chlorella were obtained due to iron supplementation. Therefore, anaerobic sludge from an industrial wastewater treatment plant (a baker’s yeast company) was pretreated to obtain an extract as a media supplement for C. sorokiniana. According to the proposed model, the sludge extract allowed us to supplement iron values close to the growth activation concentration (KFe ~12 mg L−1). Therefore, a fed-batch strategy was evaluated on nitrogen-deprived cultures supplemented with the sludge extract to promote biomass formation and fatty acid synthesis. Our findings reveal that nitrogen and iron in sludge extract can supplement heterotrophic cultures of Chlorella and provide an alternative for the valorization of industrial anaerobic sludge.

Specific growth rate of Chlamydomonas reinhardtii and Chlorella sorokiniana under medium duration light/dark cycles: 13–87 s

1999 ◽  
Vol 70 (1-3) ◽  
pp. 323-333 ◽  
Author(s):  
Marcel Janssen ◽  
Tjibbe Chris Kuijpers ◽  
Bram Veldhoen ◽  
Michel Brik Ternbach ◽  
Johannes Tramper ◽  
...  
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Chlamydomonas Reinhardtii ◽  
Specific Growth ◽  
Chlorella Sorokiniana

Examining the influence of substrates and temperature on maximum specific growth rate of denitrifiers

2006 ◽  
Vol 54 (8) ◽  
pp. 155-162 ◽  
Author(s):  
Y. Mokhayeri ◽  
A. Nichols ◽  
S. Murthy ◽  
R. Riffat ◽  
P. Dold ◽  
...  
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Growth Rates ◽  
Specific Growth ◽  
Treatment Plant ◽  
Capacity Utilization ◽  
Maximum Specific Growth Rate ◽  
Corn Syrup ◽  
Plant Uses ◽  
Specific Growth Rates

Facilities across North America are designing plants to meet stringent limits of technology (LOT) treatment for nitrogen removal (3–5 mg/L total effluent nitrogen). The anoxic capacity requirements for meeting LOT treatment are dependent on the growth rates of the denitrifying organisms. The Blue Plains Advanced Wastewater Treatment Plant (AWTP) is one of many facilities in the Chesapeake Bay region that is evaluating its ability to meet LOT treatment capability. The plant uses methanol as an external carbon source in a post-denitrification process. The process is very sensitive to denitrification in the winter. One approach to improve anoxic capacity utilization is to use an alternative substrate for denitrification in the winter to promote the growth of organisms that denitrify at higher rates. The aim of this study was to evaluate denitrification maximum specific growth rates for three substrates, acetate, corn syrup and methanol, at two temperatures (13 °C and 19 °C). These temperatures approximately reflect the minimum monthly and average annual wastewater temperature at the Blue Plains AWTP. The results suggest that the maximum specific growth rate (μmax) for corn syrup (1.3 d−1) and acetate (1.2 d−1) are higher than that for methanol (0.5 d−1) at low temperature of 13 °C. A similar trend was observed at 19 °C.

scholarly journals Requirement of Cyanobacteria Synechococcus elongatus for the biomass and lipid production

2021 ◽  
Vol 869 (1) ◽  
pp. 012042
Author(s):  
R Rusydi ◽  
A Yakupitiyage ◽  
S Pumpuang ◽  
S Mellisa ◽  
A Rahma ◽  
...  
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Lipid Content ◽  
Specific Growth ◽  
Lipid Production ◽  
Optimal Dose ◽  
Synechococcus Elongatus ◽  
Fast Growth ◽  
Good Growth ◽  
Institute Of Technology

Abstract Synechococcus elongatus is unicellularCyanobacteria having fast growth and can conduct photosynthesis process in its cells.This research purposed to evaluate the potential of Synechococcus elongatus as an candidate of biodiesel feedstocks by evaluating its biomass and lipid content in appropriate nutrients. The study was conducted at Asian Institute of Technology, Thailand. The Factorial Completely Randomized Design was used as experimental design in this study. The species was grown in BG II medium which was added by different doses of NaNO3 and KHPO4. Cultivation was conducted for seven days for several parameters, namely appropriate dose of nutrients, specific growth rate, biomass, and lipid content. This study resulted the optimal dose of nitrate from NaNO3 and phosphate from KHPO4 for good growth of Synechococcus elongatus which were 289.11 mg/L of NO3- and 22.26 mg/L of HPO4.This species grew well in BG-II medium added optimal nutrients at specific growth rate of 0.34µg/day. The optimal lipid productivity of Synechococcus elongatus was achieved at day 6th of cultivation which its dried biomass was 0.21±0.03 g/L and total lipid was 1.89±0.28%. Based on the results, Synechococcus elongatus cultivated in BG-II medium was potential tobe biodiesel source with its fast growth and lipid content in its cell.

scholarly journals PENGARUH CO2 TERHADAP LAJU PERTUMBUHAN DAN KANDUNGAN LIPID BOTRYOCOCCUS BRAUNII PADA MEDIA AIR LAUT

2016 ◽  
Vol 13 (1) ◽  
Author(s):  
Verina J. Wargadalam ◽  
Edi Saadudin ◽  
Silvy R. Fitri
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Total Lipid ◽  
Specific Growth ◽  
Sea Water ◽  
Fatty Acid Content ◽  
Lipid Production ◽  
Botryococcus Braunii ◽  
Cultivation Process ◽  
Co2 Addition

Mikroalga sebagai salah satu opsi penyedia sumber bahan bakar nabati telah banyak diteliti. Salah satu yang perlu diperhatikan untuk keberlajutannya adalah sangat besarnya kebutuhan air yang harus disediakan dalam proses budidaya. Untuk menghindari kompetisi kebutuhan air tersebut, budidaya mikroalga dalam media air laut/salin dapat dipertimbangkan. Penelitian ini mempelajari pengaruh penambahan CO2 terhadap laju pertumbuhan dan kandungan lipid mikroalga Botryococcus braunii Kützing strain NIES-836 yang dibudidaya dalam media salin dengan sistem terbuka. Penambahan CO2 sebesar 2% hingga 20% meningkatkan laju pertumbuhan spesifik, dengan nilai tertinggi dicapai pada penambahan 2% CO2, yaitu 0,53/hari, dan laju pertumbuhan mulai menurun signifikan pada penambahan 20% CO2. Produksi lipid tertinggi yang dapat dipulihkan juga dicapai pada penambahan 2% CO2, yaitu sebesar 0,34 g/L. Kandungan asam lemak dalam total lipid diketahui hanya mencapai 25% dengan komposisi utamanya Metil Palmitat, Metil Palmitoleat dan Metil Oleat.Kata kunci: Botryococcus braunii, CO2, laju pertumbuhan, lipid, air laut Microalgae as an alternative resource for biofuel have attracted many research works, and one of the concerns is its sustainability due to the need of a huge amount of water in the cultivation process. To avoid such water competition, cultivation of microalgae in media of saline/sea water can be considered. In this works, the effects of CO2 on the growth and lipid accumulation of Botryococcus braunii Kützing strain NIES-836 in sea water/saline media and open system have been studied. The CO2 additions of 2% to 20% were found to increase the specific growth rate. The highest value was reached at 2% CO2 addition, i.e.: 0.53/day, whereas, the specific growth rate started to decrease significantly at 20% CO2 addition. The highest lipid production that could be recovered was 0.34 g/L, and that was observed at 2% CO2 addition. The fatty acid content in the total lipid was 25%, mainly consists of Methyl Palmitate, Methyl Palmitoleate, and Methyl Oleate. Keywords: Botryococcus braunii, CO2, growth rate, lipid, sea water

scholarly journals KEPADATAN DAN LAJU PERTUMBUHAN SPESIFIK Nannochloropsis sp. PADA KULTIVASI HETEROTROPIK MENGGUNAKAN MEDIA HIDROLISAT SINGKONG

Omni-Akuatika ◽  
2015 ◽  
Vol 11 (2) ◽  
Author(s):  
Mujizat Kawaroe ◽  
Tri Prartono ◽  
Ganjar Saefurahman
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Culture Medium ◽  
Specific Growth ◽  
Medium Composition ◽  
Cultivation Condition ◽  
Microalgae Cultivation ◽  
Heterotrophic Cultivation ◽  
Seawater Ph ◽  
Black Plastic

Growth of Nannochloropsis sp. was observed in laboratory cultivation condition with enrichment of cassava hydrolysate into culture medium as follow : 25 ml microalgae strain, 75 ml seawater, without cassava hydrolysate (A); 25 ml microalgae strain, 25 ml cassava hydrolysate, 50 ml seawater (B), 25 ml microalgae strain, 50 ml cassava hydrolysate, 25 ml seawater (C). Cultivation condition was fixed as follow temperature 29°C, seawater pH 8, and salinity 30 ‰. Microalgae cultivation was performed at microalgae laboratory of Surfactant and Bioenergy Research Center using 100 ml Erlenmeyer covered by black plastic to prevent the influence of light. The specific growth rate of Nannochloropsis sp. was observed in 7 days for different cultivation medium composition. The highest density of microalgae was in the 4th days with 50ml cassava hydrolysate’s feeding (C treatment) about 172.661 cell/mL. The highest specific growth rate for Nannochloropsis sp. cultivation was observed in the 6th days without cassava hydrolysate’s feeding. Result of statistical analysis showed that different cassava hydrolysate’s feeding treatments (ml) in heterotrophically microalgae cultivation influenced the density of microalgae (cell/mL) but not influenced the specific growth rate of microalgae (per day).Keywords: Cassava hydrolysate, Density, Heterotrophic cultivation, Spesific growth rate, Nannochloropsis sp.

scholarly journals Growth and secondary metabolites content of chloroform extract of Chlorella sp. and Chlorella sorokiniana cultured on chicken broiler waste media

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Devy Susanty ◽  
Ade Ayu Oksari
Keyword(s):  
Growth Rate ◽  
Secondary Metabolites ◽  
Specific Growth Rate ◽  
Cell Density ◽  
Specific Growth ◽  
Culture Media ◽  
Chloroform Extract ◽  
Chlorella Sorokiniana ◽  
Phytochemical Content ◽  
Chlorella Sp

Abstract. Susanty D, Oksari AA. 2020. Growth and secondary metabolites content of chloroform extract of Chlorella sp. and Chlorella sorokiniana cultured on chicken broiler waste media. Nusantara Bioscience 12: 28-32. Intensive chicken broiler farms create waste in the environment. Chicken Broiler Waste (CBW) was used as culture media for Chlorella sp. InaCC M39 and Chlorella sorokiniana InaCC M38 at various concentrations (2, 4, 6, 8, and 10%), and compared to AF6 media. The growth of Chlorella was observed every 48 hours for 10 days by counting the number of cells using a hemocytometer. The chloroform extract of Chlorella sp. and C. sorokiniana were analyzed for their phytochemical content to determine the presence of alkaloids, flavonoids, saponin, steroids, and tannin. The results showed that 2% of CBW media was the best medium for the growth of Chlorella with nitrogen (N), phosphorus (P), and potassium (K) content was 0.01% N; 0.01% P; 0.06% K respectively. The highest specific growth rate of Chlorella sp. on 2% CBW medium was on the 8th day of culture (0.8133) with cell density of 1.6 x 106 cells/mL, while the highest specific growth rate of Chlorella sorokiniana was on the 6th day (0.8907) with cell density of 2.99 x 107 cells/mL. The chloroform extract of the biomass of Chlorella sorokiniana contained steroid and saponin

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