scholarly journals Evaluation of Peripheral Blood and Cord Blood Platelet Lysates in Isolation and Expansion of Multipotent Mesenchymal Stromal Cells

2018 ◽  
Vol 5 (1) ◽  
pp. 19 ◽  
Author(s):  
Ioanna Christou ◽  
Panagiotis Mallis ◽  
Efstathios Michalopoulos ◽  
Theofanis Chatzistamatiou ◽  
George Mermelekas ◽  
...  
Keyword(s):  
Cord Blood ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Peripheral Blood ◽  
Blood Platelet ◽  
Multipotent Mesenchymal Stromal Cells

scholarly journals Homing and Engraftment of Intravenously Administered Equine Cord Blood-Derived Multipotent Mesenchymal Stromal Cells to Surgically Created Cutaneous Wound in Horses: A Pilot Project

Cells ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 1162
Author(s):  
Suzanne J. K. Mund ◽  
Eiko Kawamura ◽  
Awang Hazmi Awang-Junaidi ◽  
John Campbell ◽  
Bruce Wobeser ◽  
...  
Keyword(s):  
Cord Blood ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Adverse Reactions ◽  
Pilot Project ◽  
Multipotent Mesenchymal Stromal Cells ◽  
The Body ◽  
Laboratory Animals ◽  
Clinical Safety ◽  
Allogeneic Mscs

Limb wounds on horses are often slow to heal and are prone to developing exuberant granulation tissue (EGT) and close primarily through epithelialization, which results in a cosmetically inferior and non-durable repair. In contrast, wounds on the body heal rapidly and primarily through contraction and rarely develop EGT. Intravenous (IV) multipotent mesenchymal stromal cells (MSCs) are promising. They home and engraft to cutaneous wounds and promote healing in laboratory animals, but this has not been demonstrated in horses. Furthermore, the clinical safety of administering >1.00 × 108 allogeneic MSCs IV to a horse has not been determined. A proof-of-principle pilot project was performed with two horses that were administered 1.02 × 108 fluorescently labeled allogeneic cord blood-derived MSCs (CB-MSCs) following wound creation on the forelimb and thorax. Wounds and contralateral non-wounded skin were sequentially biopsied on days 0, 1, 2, 7, 14, and 33 and evaluated with confocal microscopy to determine presence of homing and engraftment. Results confirmed preferential homing and engraftment to wounds with persistence of CB-MSCs at 33 days following wound creation, without clinically adverse reactions to the infusion. The absence of overt adverse reactions allows further studies to determine effects of IV CB-MSCs on equine wound healing.

Cord blood CD34+ cells expanded on Wharton's jelly multipotent mesenchymal stromal cells improve the hematopoietic engraftment in NOD/SCID mice

2014 ◽  
Vol 93 (5) ◽  
pp. 384-391 ◽  
Author(s):  
Luisa Milazzo ◽  
Francesca Vulcano ◽  
Alessandra Barca ◽  
Giampiero Macioce ◽  
Emanuela Paldino ◽  
...  
Keyword(s):  
Cord Blood ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Scid Mice ◽  
Wharton’S Jelly ◽  
Wharton's Jelly ◽  
Cd34 Cells ◽  
Cord Blood Cd34

scholarly journals Parameters that influence the isolation of multipotent mesenchymal stromal cells from human umbilical cord blood

2013 ◽  
Vol 6 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Attiyeh Vasaghi ◽  
Atefeh Dehghani ◽  
Zeinab Khademalhosseini ◽  
Mohsen Khosravi Maharlooei ◽  
Ahmad Monabati ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Mesenchymal Stromal Cells ◽  
Umbilical Cord Blood ◽  
Stromal Cells ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord

Humanized system to propagate cord blood-derived multipotent mesenchymal stromal cells for clinical application

2007 ◽  
Vol 2 (4) ◽  
pp. 371-382 ◽  
Author(s):  
Andreas Reinisch ◽  
Christina Bartmann ◽  
Eva Rohde ◽  
Katharina Schallmoser ◽  
Vesna Bjelic-Radisic ◽  
...  
Keyword(s):  
Cord Blood ◽  
Clinical Application ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Multipotent Mesenchymal Stromal Cells

scholarly journals Dynamics of Cytokines Concentration in the Blood Plasma of Patients after Multipotent Mesenchymal Stromal Cells Administration for Graft Versus Host Disease Prevention

Blood ◽  
2021 ◽  
Vol 138 (Supplement 1) ◽  
pp. 1101-1101
Author(s):  
Nataliya A. Petinati ◽  
Julia O Davydova ◽  
Kseniya A. Nikiforova ◽  
Alexey Bigildeev ◽  
Georgiy Arapidi ◽  
...  
Keyword(s):  
T Cells ◽  
Mesenchymal Stromal Cells ◽  
Graft Versus Host Disease ◽  
Stromal Cells ◽  
Peripheral Blood ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Control Group ◽  
Allogeneic Bone ◽  
Host Disease ◽  
Graft Versus Host

Abstract Introduction Despite the large number of clinical studies on the use of multipotent mesenchymal stromal cells (MSCs) for the treatment and prevention of graft-versus-host disease (GVHD), the mechanism of their action in the organism is not well understood. The known data refer either to clinical effects or obtained in vitro. Due to the immunomodulatory effect of MSCs in the body, subpopulations of T cells and the concentration of cytokines involved in the immune response can change. The role of T cells and certain cytokines (TNF alpha, IL6, IL8, etc.) in the pathophysiology of GVHD is described. The aim of this investigation was to study the composition of T cells subpopulations and the concentration of cytokines in the peripheral blood of patients who received MSCs for GVHD prophylaxis. Methods The study included 21 patients who received hematopoietic stem cells donor's derived MSCs for the prevention of GVHD as part of the ClinicalTrials.gov Identifier NCT01941394 trial. The control group included 16 patients who did not receive MSCs. After signing informed consent, blood samples were taken from all patients during routine examinations on the day of restoration of the number of leukocytes to 1000 in μl (day 0), after 3 and after 30 days. MSCs were injected on day 0. None of the patients developed GVHD during this time. To analyze plasma cytokines and chemokines, the Bio-Plex Pro Human Cytokine Panel kit, 27-Plex (BioRad) was used, to determine the concentrations of IL-1β, IL-1ra, IL-2, IL-4, IL-5, IL- 6, IL-7, IL-8, IL-9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17, bFGF, Eotaxin, G-CSF, GM-CSF, IFN-γ, IP-10, MCP-1 (MCAF), MIP-1α, MIP-1β, PDGF-bb, RANTES, TNF-α, VEGF according to manufacturer's recommendations. Forward and side scattering parameters determined the peripheral blood lymphocytes population and then CD4+ or CD8+ lymphocytes were gated. For each of this population the composition of memory cells subset were determined by flow cytometry. Results Significant differences were found between the 2 groups only on day 30 in the concentration of IL8 (17.0±3.2 pg/ml in the control group versus 32.8±4.1 pg/ml in the MSC group, p<0.0001). It has been shown that 30 days after MSCs, the number of CD4+ T cells in the peripheral blood of patients significantly increases compared to the group without MSCs (CD4 CM 38.9±9.0 vs 22.4±7.8, CD4 TM 97.0±32.5 vs 91.0±43.2, CD4 TE 5.0±1.9 vs 1.4±1.0, CD4 EM 34.6±20.1 vs 19.9±7.9, CD4CD25+ 27.6±6.6 vs 12.9±5.0). These cells produce IL8, which play an important role in immune cell homeostasis by activating antimicrobial neutrophils. Without the introduction of MSCs, the concentration of this protective against GVHD cytokine practically did not change within a month, whereas after the introduction of MSCs, it gradually increased almost 2 times. However, the dynamics of changes in the levels of the studied cytokines differed greatly between the 2 groups (Table). The concentration of IP10, which is involved in the development of GVHD, increased significantly faster and stronger in the group without MSCs. An increase in the concentration of other investigated cytokines associated with the activation of macrophages (MCP-1, MIP-1a, MIP-1b) did not depend on the MSCs administration. At the same time, in the MSC group, the concentration of the growth factor PDGF-bb necessary for the HSC proliferation increased significantly more actively. The IL9 concentration on day 0 was comparable to the level in healthy donors, and then gradually increased in both groups and after 30 days it was significantly higher than on day 0 in the MSC administration group. The concentration of G-CSF changed in a similar way. Conclusion Changes in the dynamics of T cells subpopulations and the concentration of cytokines in the blood after MSCs administration contribute to a faster recovery of patients after allogeneic bone marrow transplantation. The work were supported by the Russian Foundation for Basic Research, Project No. 19-29-04023. Figure 1 Figure 1. Disclosures No relevant conflicts of interest to declare.

scholarly journals Isolation and characterization of equine peripheral blood-derived multipotent mesenchymal stromal cells

2013 ◽  
Vol 33 (9) ◽  
pp. 1151-1154 ◽  
Author(s):  
Armando de M. Carvalho ◽  
Ana Lucia M. Yamada ◽  
Juliana R.B. Martins ◽  
Leandro Maia ◽  
Marjorie A. Golim ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Peripheral Blood ◽  
Chondrogenic Differentiation ◽  
Mononuclear Cells ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Isolation And Characterization ◽  
Oil Red O ◽  
Promising Type

The objective of the study was to isolate, cultivate and characterize equine peripheral blood-derived multipotent mesenchymal stromal cells (PbMSCs). Peripheral blood was collected, followed by the isolation of mononuclear cells using density gradient reagents, and the cultivation of adherent cells. Monoclonal mouse anti-horse CD13, mouse anti-horse CD44, and mouse anti-rat CD90 antibodies were used for the immunophenotypic characterization of the surface of the PbMSCs. These cells were also cultured in specific media for adipogenic and chondrogenic differentiation. There was no expression of the CD13 marker, but CD44 and CD90 were expressed in all of the passages tested. After 14 days of cell differentiation into adipocytes, lipid droplets were observed upon Oil Red O (ORO) staining. Twenty-one days after chondrogenic differentiation, the cells were stained with Alcian Blue. Although the technique for the isolation of these cells requires improvement, the present study demonstrates the partial characterization of PbMSCs, classifying them as a promising type of progenitor cells for use in equine cell therapy.

Mesenchymal stromal cells promote or suppress the proliferation of T lymphocytes from cord blood and peripheral blood: the importance of low cell ratio and role of interleukin-6

Cytotherapy ◽  
2009 ◽  
Vol 11 (5) ◽  
pp. 570-583 ◽  
Author(s):  
Mehdi Najar ◽  
Redouane Rouas ◽  
Gordana Raicevic ◽  
Hichame Id Boufker ◽  
Philippe Lewalle ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Cord Blood ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Interleukin 6 ◽  
Peripheral Blood ◽  
Cell Ratio

scholarly journals Peripheral Blood Derived Mononuclear Cells Enhance the Migration and Chondrogenic Differentiation of Multipotent Mesenchymal Stromal Cells

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Niina Hopper ◽  
John Wardale ◽  
Daniel Howard ◽  
Roger Brooks ◽  
Neil Rushton ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Cartilage Repair ◽  
Stromal Cells ◽  
Peripheral Blood ◽  
Chondrogenic Differentiation ◽  
Mononuclear Cells ◽  
Test Group ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Infrapatellar Fat Pad ◽  
Scratch Assay

A major challenge in cartilage repair is the lack of chondrogenic cells migrating from healthy tissue into damaged areas and strategies to promote this should be developed. The aim of this study was to evaluate the effect of peripheral blood derived mononuclear cell (PBMC) stimulation on mesenchymal stromal cells (MSCs) derived from the infrapatellar fat pad of human OA knee. Cell migration was measured using an xCELLigence electronic migration chamber system in combination with scratch assays. Gene expression was quantified with stem cell PCR arrays and validated using quantitative real-time PCR (rtPCR). In both migration assays PBMCs increased MSC migration by comparison to control. In scratch assay the wound closure was 55% higher after 3 hours in the PBMC stimulated test group (P=0.002), migration rate was 9 times faster (P=0.008), and total MSC migration was 25 times higher after 24 hours (P=0.014). Analysis of MSCs by PCR array demonstrated that PBMCs induced the upregulation of genes associated with chondrogenic differentiation over 15-fold. In conclusion, PBMCs increase both MSC migration and differentiation suggesting that they are an ideal candidate for inclusion in regenerative medicine therapies aimed at cartilage repair.

Effect of Cord Blood Platelet Gel on wound healing capacity of human Mesenchymal Stromal Cells

2020 ◽  
Vol 59 (3) ◽  
pp. 102734
Author(s):  
Panagiotis Mallis ◽  
Vivian Alevrogianni ◽  
Phaedra Sarri ◽  
Athanassios D. Velentzas ◽  
Catherine Stavropoulos-Giokas ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cord Blood ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Blood Platelet ◽  
Platelet Gel ◽  
Human Mesenchymal Stromal Cells
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