scholarly journals Construction and Experimental Validation of a Quantitative Kinetic Model of Nitric Oxide Stress in Enterohemorrhagic Escherichia coli O157:H7

2016 ◽  
Vol 3 (1) ◽  
pp. 9 ◽  
Author(s):  
Jonathan Robinson ◽  
Mark Brynildsen
Keyword(s):  
Nitric Oxide ◽  
Escherichia Coli ◽  
Kinetic Model ◽  
Experimental Validation ◽  
Enterohemorrhagic Escherichia Coli ◽  
Escherichia Coli O157 ◽  
Oxide Stress

scholarly journals Quantification of enterohemorrhagic Escherichia coli O157:H7 protein abundance by high-throughput proteome

PLoS ONE ◽  
2018 ◽  
Vol 13 (12) ◽  
pp. e0208520 ◽  
Author(s):  
Wanderson Marques Da Silva ◽  
Jinlong Bei ◽  
Natalia Amigo ◽  
María Pía Valacco ◽  
Ariel Amadio ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
High Throughput ◽  
Enterohemorrhagic Escherichia Coli ◽  
Escherichia Coli O157 ◽  
Protein Abundance

Enterohemorrhagic Escherichia coli O157 Outbreak in Obihiro-City

1998 ◽  
Vol 72 (2) ◽  
pp. 89-96
Author(s):  
Sou-ichi MAKINO ◽  
Hiroshi ASAKURA ◽  
Toshikazu SHIRAHATA ◽  
Tetsuya IKEDA ◽  
Koichi TAKESHI ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Enterohemorrhagic Escherichia Coli ◽  
Escherichia Coli O157

Repression of flagella motility in enterohemorrhagic Escherichia coli O157:H7 by mucin components

2012 ◽  
Vol 423 (4) ◽  
pp. 789-792 ◽  
Author(s):  
Jong Chul Kim ◽  
Jang W. Yoon ◽  
Cheorl-Ho Kim ◽  
Mi-Sun Park ◽  
Seung-Hak Cho
Keyword(s):  
Escherichia Coli ◽  
Enterohemorrhagic Escherichia Coli ◽  
Escherichia Coli O157

scholarly journals Correlation of enterohemorrhagic Escherichia coli O157 prevalence in feces, hides, and carcasses of beef cattle during processing

2000 ◽  
Vol 97 (7) ◽  
pp. 2999-3003 ◽  
Author(s):  
R. O. Elder ◽  
J. E. Keen ◽  
G. R. Siragusa ◽  
G. A. Barkocy-Gallagher ◽  
M. Koohmaraie ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Beef Cattle ◽  
Enterohemorrhagic Escherichia Coli ◽  
Escherichia Coli O157

scholarly journals Cooperative Roles of Nitric Oxide-Metabolizing Enzymes To Counteract Nitrosative Stress in Enterohemorrhagic Escherichia coli

2019 ◽  
Vol 87 (9) ◽  
Author(s):  
Takeshi Shimizu ◽  
Akio Matsumoto ◽  
Masatoshi Noda
Keyword(s):  
Nitric Oxide ◽  
Escherichia Coli ◽  
Stationary Phase ◽  
Nitrosative Stress ◽  
Enterohemorrhagic Escherichia Coli ◽  
The Other ◽  
Anaerobic Growth ◽  
Bacterial Cells ◽  
Metabolizing Enzymes ◽  
Microaerobic Conditions

ABSTRACT Enterohemorrhagic Escherichia coli (EHEC) has at least three enzymes, NorV, Hmp, and Hcp, that act independently to lower the toxicity of nitric oxide (NO), a potent antimicrobial molecule. This study aimed to reveal the cooperative roles of these defensive enzymes in EHEC against nitrosative stress. Under anaerobic conditions, combined deletion of all three enzymes significantly increased the NO sensitivity of EHEC determined by the growth at late stationary phase; however, the expression of norV restored the NO resistance of EHEC. On the other hand, the growth of Δhmp mutant EHEC was inhibited after early stationary phase, indicating that NorV and Hmp play a cooperative role in anaerobic growth. Under microaerobic conditions, the growth of Δhmp mutant EHEC was inhibited by NO, indicating that Hmp is the enzyme that protects cells from NO stress under microaerobic conditions. When EHEC cells were exposed to a lower concentration of NO, the NO level in bacterial cells of Δhcp mutant EHEC was higher than those of the other EHEC mutants, suggesting that Hcp is effective at regulating NO levels only at a low concentration. These findings of a low level of NO in bacterial cells with hcp indicate that the NO consumption activity of Hcp was suppressed by Hmp at a low range of NO concentrations. Taken together, these results show that the cooperative effects of NO-metabolizing enzymes are regulated by the range of NO concentrations to which the EHEC cells are exposed.

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