scholarly journals Combined Effect of Spirulina Platensis and Punica Granatum Peel Extacts: Phytochemical Content and Antiphytophatogenic Activity

2019 ◽  
Vol 9 (24) ◽  
pp. 5475 ◽  
Author(s):  
Hajer Ben Hlima ◽  
Thouraya Bohli ◽  
Mariem Kraiem ◽  
Abdelmottaleb Ouederni ◽  
Lotfi Mellouli ◽  
...  
Keyword(s):  
Spirulina Platensis ◽  
Marine Algae ◽  
Synergistic Effects ◽  
Fusarium Culmorum ◽  
Punica Granatum ◽  
Plant Diseases ◽  
Pomegranate Peel ◽  
Total Polyphenols ◽  
Phytochemical Content ◽  
By Products

Biological control is one of the effective methods for managing plant diseases in food production and quality. In fact, there is a growing trend to find new bio-sources, such as marine algae and vegetal by-products. In this study, pomegranate (Punica granatum) peel (S1) and Spirulina platensis (S2) alone and in combinations, pomegranate peel/Spirulina: 25%/75% (S3) and 50%/50% (S4) were evaluated for antimycotoxigenic and antiphytopathogenic fungal properties. The chemical composition (moisture, dry matter, protein, lipid and ash) as well as total polyphenols, flavonoids and anthocyanins content were evaluated in the four extracts. Using agar diffusion and broth microdilution methods, the anti Fusarium oxysporum, Fusarium culmorum, Fusarium graminearum, Aspergillus niger and Alternaria alternata activities were measured and their correlations with phytochemical content were evaluated. Interestingly, combinations between Spirulina at 75% and pomegranate peel at 25% (S3) have a significant impact (p < 0.05) on the antifungal activity compared to S1, S2 and S4. These findings underlie the effectiveness of biocontrols over standard fungicides and imply that existing methods can be further improved by synergistic effects while maintaining food safety in an eco-friendly manner.

scholarly journals Variation of the Polyphenolic Composition and Antioxidant Capacity of Freshly Prepared Pomegranate Leaf Infusions over One-Day Storage

Antioxidants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1187
Author(s):  
Manyou Yu ◽  
Irene Gouvinhas ◽  
Ana Barros
Keyword(s):  
Antioxidant Capacity ◽  
Gallic Acid ◽  
Antioxidant Properties ◽  
Punica Granatum ◽  
Added Value ◽  
Plant Materials ◽  
Polyphenolic Composition ◽  
Punica Granatum L ◽  
Intensive Search ◽  
By Products

In recent decades, an intensive search for natural and novel types of antioxidant polyphenolics has been carried out on numerous plant materials. However, the current literature has very little information on their storage stability in the form of freshly prepared infusions. This study aims to characterize the polyphenolic composition and the antioxidant capacity of pomegranate (Punica granatum L.) leaf infusions over one-day storage (analyzed at 0, 2, 4, 6, 8, and 24 h). Spectrophotometric evaluation demonstrated that the infusion presented no significant changes in the content of total phenols (131.40–133.47 mg gallic acid g−1) and ortho-diphenols (239.91–244.25 mg gallic acid g−1). The infusion also maintained high stability (over 98% and 82%, respectively) for flavonoids (53.30–55.84 mg rutin g−1) and condensed tannins (102.15–124.20 mg epicatechin g−1), with stable (>90%) potent antioxidant capacity (1.5–2.2 mmol Trolox g−1) throughout 0–24 h storage. The main decrease was observed during 0–2 h storage of flavonoids, 8–24 h storage of tannins, and 0–4 h storage of antioxidant capacity. Chromatographic analysis further revealed that 7 decreased and 11 increased compounds were found within 0–24 h storage. The good stability of the total polyphenolics and antioxidant properties might be related to the complex conversion and activity compensation among these compounds. The findings suggest that pomegranate leaf infusion could be of great interest in the valorization of high added-value by-products and in the application of green and functional alternatives in the food-pharma and nutraceutical industries.

Optimization of subcritical water extraction of phenolic antioxidants from pomegranate (Punica granatum L.) peel by response surface methodology

2017 ◽  
Vol 9 (32) ◽  
pp. 4647-4656 ◽  
Author(s):  
Linlin Yan ◽  
Yungang Cao ◽  
Guangyao Zheng
Keyword(s):  
Response Surface Methodology ◽  
Subcritical Water ◽  
Punica Granatum ◽  
Water Extraction ◽  
Phenolic Antioxidants ◽  
Subcritical Water Extraction ◽  
Extraction Technology ◽  
Pomegranate Peel ◽  
Efficient Extraction ◽  
Punica Granatum L

Subcritical water extraction (SWE), a ‘green’ and efficient extraction technology, was applied to extract phenolic antioxidants from pomegranate peel in this study.

Punica granatum peel extracts mediated the green synthesis of gold nanoparticles and their detailed in vivo biological activities

2021 ◽  
Vol 10 (1) ◽  
pp. 882-892
Author(s):  
Sami Bawazeer ◽  
Abdur Rauf ◽  
Taufiq Nawaz ◽  
Anees Ahmed Khalil ◽  
Muhammad Sameem Javed ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Muscle Relaxant ◽  
Biological Activities ◽  
Punica Granatum ◽  
Environmental Safety ◽  
Surface Plasma Resonance ◽  
Pomegranate Peel ◽  
Synthesis Of Nanoparticles ◽  
Vis Spectroscopy

Abstract Requirements for developing new methodologies to biosynthesize nanoparticles are increasing day by day. The typical chemical synthesis of nanoparticles has raised concerns regarding environmental safety and adverse impact on human health. Therefore, there is an urgent need to develop green synthesized nanoparticles that are considered to be safe, ecofriendly, and cost-effective as compared to chemical approaches. Hence, in this study, we synthesized and characterized pomegranate peel extract-based gold nanoparticles (PP-AuNPs) through UV-visible spectroscopy, FT-IR, and AFM microscopy. Furthermore, the biological activities like analgesic, muscle relaxant, and sedative properties of synthesized PP-AuNPs were also determined. The change of color to dark ruby indicates the formation of AuNPs. The surface plasma resonance (SPR) peak in the absorption spectra was shown at 525 nm by using (UV-Vis) spectroscopy. A single distinctive peak implied the shape of nanoparticles to be spherical. AFM images revealed that the biosynthesized nanoparticles were spherical in shape. Furthermore, the images confirm the uniform distribution of PP-AuNPs with particle sizes ranging from 4 to 16 nm. Different classes of phytochemicals were preliminarily identified in extracts. The analgesic effect of extracts (70.04%) and PP-AuNPs (81.98%) demonstrated a significant (p < 0.001) percent reduction in writhing at a dose of 100 and 15 mg·kg−1, respectively. A mild muscle relaxant effect was noted against both the tested samples while a significant sedative effect was observed for both samples; however, PP-AuNPs weres more sedative compared to the extract. Pomegranate peel extracts and synthesized PP-AuNPs were found to possess significant analgesic, muscle relaxant, and sedative properties.

Anti-Candida Activity of Pomegranate Peel Extract in Comparison with Curcumin Extract and their Synergism with Fluconazole and Nystatin

2021 ◽  
Vol 30 (2) ◽  
pp. 81-87
Author(s):  
Sara Y. Maxwell ◽  
Sally Elnawasany ◽  
Azza M. Hassan ◽  
Marwa M. E. Abd-Elmonsef
Keyword(s):  
Candida Species ◽  
Synergistic Effects ◽  
Systemic Disease ◽  
Antifungal Drugs ◽  
Diffusion Method ◽  
Oropharyngeal Candidiasis ◽  
Pomegranate Peel ◽  
Methanolic Extracts ◽  
Pomegranate Peel Extract

Background: Oropharyngeal candidiasis is an important sign that may reflect a serious systemic disease, especially in immunocompromised patients who face the intolerable side effects of the available antifungal drugs. This necessitates the development of safe and effective natural components. Objectives: to evaluate the in vitro activities of both pomegranate peel and curcumin extracts and to compare them with nystatin and fluconazole drugs against Candida species. As far as we know, this is the first study comparing between the antifungal potency of both extracts. Methodology: Different Candida species were isolated from patients with oropharyngeal candidiasis. The antifungal activities of methanolic extracts of pomegranate peel and curcumin were tested by disc diffusion method. Both extracts were added to each of nystatin and fluconazole discs to measure their synergistic effects. Results: Highly significant synergism was detected between both extracts and each of antifungal drugs. Curcumin extract was more potent than pomegranate extract. Conclusion: When used in combination with nystatin and fluconazole, curcumin and pomegranate peel extracts are promising and effective anti-Candida agents.

scholarly journals Nickel Effect on Pomegranate Cracking, Nutrient Concentrations, and Biochemical Parameters of Pomegranate Peel

HortScience ◽  
2018 ◽  
Vol 53 (11) ◽  
pp. 1677-1682
Author(s):  
Olga Dichala ◽  
Ioannis Therios ◽  
Magdalene Koukourikou-Petridou ◽  
Aristotelis Papadopoulos
Keyword(s):  
Field Experiment ◽  
Boric Acid ◽  
Biochemical Parameters ◽  
Calcium Concentration ◽  
Punica Granatum ◽  
Nutrient Concentrations ◽  
Crop Loss ◽  
Fruit Maturation ◽  
Fruit Peel ◽  
Pomegranate Peel

A field experiment was conducted in a pomegranate (Punica granatum L.) orchard of the well-known cultivars Wonderful and Acco, located in the farm of Aristotle University. The trees were sprayed, every 15 days from flowering (April) to fruit maturation (September), with solutions containing 0, 25, 50, 100 μm Ni, and 100 μm Ni + 100 μm B prepared with Ni(NO3)2·6H2O and boric acid. Leaves and fully ripe fruits were initially sorted into cracked and uncracked ones, then further separated into peel and seeds, sampled, and analyzed. Nickel sprays were effective in controlling fruit splitting as well as Ca and Mg concentration of fruit peels. The correlation between cracking level and Ni concentration in solution was linear and negative. Cracking percentage with 50 μm Ni was lower in ‘Wonderful’, whereas no difference was recorded between the cultivars in the remaining treatments. Leaves had the smallest Ni concentration compared with fruit peel and seeds. Calcium concentration of pomegranate peels was higher than that of control peel at 50 μm Ni in ‘Wonderful’. Concerning ‘Acco’, the treatments 25 μm Ni, 50 μm Ni, and 100 μm Ni + 100 μm B reduced Ca concentration, compared with control. ‘Wonderful’ fruit peel contained more phenolics than ‘Acco’. The treatments 25, 50, and 100 μm Ni increased significantly the flavonoid concentration of fruit peels. The antioxidant capacity ferric-reducing antioxidant potential (FRAP) was linearly increased with Ni concentration in solution in ‘Wonderful’, whereas in ‘Acco’ it decreased at 25 and 50 μm Ni. Our data indicates that improving Ni nutrition of pomegranate can potentially reduce crop loss due to cracking and modified phenol and flavonoid concentration and FRAP value of fruit peel.

scholarly journals Volatile Organic Compound Profiles From Wheat Diseases Are Pathogen-Specific and Can Be Exploited for Disease Classification

2022 ◽  
Vol 12 ◽  
Author(s):  
Andrea Ficke ◽  
Belachew Asalf ◽  
Hans Ragnar Norli
Keyword(s):  
Pure Culture ◽  
Fusarium Culmorum ◽  
Fungal Species ◽  
Plant Diseases ◽  
Disease Classification ◽  
Diagnostic Tools ◽  
Head Blight ◽  
Wheat Varieties ◽  
Wheat Diseases ◽  
Volatile Organic

Plants and fungi emit volatile organic compounds (VOCs) that are either constitutively produced or are produced in response to changes in their physico-chemical status. We hypothesized that these chemical signals could be utilized as diagnostic tools for plant diseases. VOCs from several common wheat pathogens in pure culture (Fusarium graminearum, Fusarium culmorum, Fusarium avenaceum, Fusarium poae, and Parastagonospora nodorum) were collected and compared among isolates of the same fungus, between pathogens from different species, and between pathogens causing different disease groups [Fusarium head blight (FHB) and Septoria nodorum blotch (SNB)]. In addition, we inoculated two wheat varieties with either F. graminearum or P. nodorum, while one variety was also inoculated with Blumeria graminis f.sp. tritici (powdery mildew, PM). VOCs were collected 7, 14, and 21 days after inoculation. Each fungal species in pure culture emitted a different VOC blend, and each isolate could be classified into its respective disease group based on VOCs with an accuracy of 71.4 and 84.2% for FHB and SNB, respectively. When all collection times were combined, the classification of the tested diseases was correct in 84 and 86% of all cases evaluated. Germacrene D and sativene, which were associated with FHB infection, and mellein and heptadecanone, which were associated with SNB infection, were consistently emitted by both wheat varieties. Wheat plants infected with PM emitted significant amounts of 1-octen-3-ol and 3,5,5-trimethyl-2-hexene. Our study suggests that VOC blends could be used to classify wheat diseases. This is the first step toward a real-time disease detection in the field based on chemical signatures of wheat diseases.

High potential of agro-industrial by-products of pomegranate (Punica granatum L.) as the powerful antifungal and antioxidant substances

2011 ◽  
Vol 34 (3) ◽  
pp. 1523-1527 ◽  
Author(s):  
Ali Tehranifar ◽  
Yahya Selahvarzi ◽  
Mahdiyeh Kharrazi ◽  
Vahid Jahan Bakhsh
Keyword(s):  
Punica Granatum ◽  
High Potential ◽  
Punica Granatum L ◽  
By Products

Fusarium culmorum multi-toxin screening in malting and brewing by-products

LWT ◽  
2018 ◽  
Vol 98 ◽  
pp. 642-645 ◽  
Author(s):  
Kristina Mastanjević ◽  
Bojan Šarkanj ◽  
Benedikt Warth ◽  
Rudolf Krska ◽  
Michael Sulyok ◽  
...  
Keyword(s):  
Fusarium Culmorum ◽  
By Products
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