scholarly journals Potential of Fluorescence Index Derived from the Slope Characteristics of Laser-Induced Chlorophyll Fluorescence Spectrum for Rice Leaf Nitrogen Concentration Estimation

2019 ◽  
Vol 9 (5) ◽  
pp. 916 ◽  
Author(s):  
Jian Yang ◽  
Lin Du ◽  
Shuo Shi ◽  
Wei Gong ◽  
Jia Sun ◽  
...  

Leaf nitrogen concentration (LNC) is a major biochemical parameter for estimating photosynthetic efficiency and crop yields. Laser-induced fluorescence, which is a promising potential technology, has been widely used to estimate the growth status of crops with the help of multivariate analysis. In this study, a fluorescence index was proposed based on the slope characteristics of fluorescence spectrum and was used to estimate LNC. Then, the performance of different fluorescence characteristics (proposed fluorescence index, fluorescence ratios, and fluorescence characteristics calculated by principal component analysis (PCA)) for LNC estimation was analyzed based on back-propagation neural network (BPNN) model. The proposed fluorescence index exhibited more stability and reliability for LNC estimation than fluorescence ratios and characteristics calculated by PCA. In addition, the effect of different kernel functions and hidden layer sizes of BPNN model on the accuracy of LNC estimation was discussed for different fluorescence characteristics. The optimal train functions “trainrp,” “trainbr,” and “trainlm” were then selected with higher R2 and lower standard deviation (SD) values than those of other train functions. In addition, experimental results demonstrated that the hidden layer size has a smaller impact on the accuracy of LNC estimation than the kernel function of the BPNN model.

2020 ◽  
Vol 7 (2) ◽  
pp. 191941
Author(s):  
Jian Yang ◽  
Lin Du ◽  
Wei Gong ◽  
Shuo Shi ◽  
Jia Sun

Leaf nitrogen concentration (LNC) is a major indicator in the estimation of the crop growth status which has been diffusely applied in remote sensing. Thus, it is important to accurately obtain LNC by using passive or active technology. Laser-induced fluorescence can be applied to monitor LNC in crops through analysing the changing of fluorescence spectral information. Thus, the performance of fluorescence spectrum (FS) and first-derivative fluorescence spectrum (FDFS) for paddy rice (Yangliangyou 6 and Manly Indica) LNC estimation was discussed, and then the proposed FS + FDFS was used to monitor LNC by multivariate analysis. The results showed that the difference between FS ( R 2 = 0.781, s.d. = 0.078) and FDFS ( R 2 = 0.779, s.d. = 0.097) for LNC estimation by using the artificial neural network is not obvious. The proposed FS + FDFS can improved the accuracy of LNC estimation to some extent ( R 2 = 0.813, s.d. = 0.051). Then, principal component analysis was used in FS and FDFS, and extracted the main fluorescence characteristics. The results indicated that the proposed FS + FDFS exhibited higher robustness and stability for LNC estimation ( R 2 = 0.851, s.d. = 0.032) than that only using FS ( R 2 = 0.815, s.d. = 0.059) or FDFS ( R 2 = 0.801, s.d. = 0.065).


2018 ◽  
Vol 10 (9) ◽  
pp. 1402 ◽  
Author(s):  
Jian Yang ◽  
Shalei Song ◽  
Lin Du ◽  
Shuo Shi ◽  
Wei Gong ◽  
...  

Leaf nitrogen concentration (LNC) is a significant indicator of crops growth status, which is related to crop yield and photosynthetic efficiency. Laser-induced fluorescence is a promising technology for LNC estimation and has been widely used in remote sensing. The accuracy of LNC monitoring relies greatly on the selection of fluorescence characteristics and the number of fluorescence characteristics. It would be useful to analyze the performance of fluorescence intensity and ratio characteristics at different wavelengths for LNC estimation. In this study, the fluorescence spectra of paddy rice excited by different excitation light wavelengths (355 nm, 460 nm, and 556 nm) were acquired. The performance of the fluorescence intensity and fluorescence ratio of each band were analyzed in detail based on back-propagation neural network (BPNN) for LNC estimation. At 355 nm and 460 nm excitation wavelengths, the fluorescence characteristics related to LNC were mainly located in the far-red region, and at 556 nm excitation wavelength, the red region being an optimal band. Additionally, the effect of the number of fluorescence characteristics on the accuracy of LNC estimation was analyzed by using principal component analysis combined with BPNN. Results demonstrate that at least two fluorescence spectral features should be selected in the red and far-red regions to estimate LNC and efficiently improve the accuracy of LNC estimation.


2019 ◽  
Vol 27 (4) ◽  
pp. 3978 ◽  
Author(s):  
Jian Yang ◽  
Lin Du ◽  
Wei Gong ◽  
Shuo Shi ◽  
Jia Sun ◽  
...  

2014 ◽  
Vol 38 (6) ◽  
pp. 640-652 ◽  
Author(s):  
YAN Shuang ◽  
◽  
ZHANG Li ◽  
JING Yuan-Shu ◽  
HE Hong-Lin ◽  
...  

2015 ◽  
Vol 7 (11) ◽  
pp. 14939-14966 ◽  
Author(s):  
Xia Yao ◽  
Yu Huang ◽  
Guiyan Shang ◽  
Chen Zhou ◽  
Tao Cheng ◽  
...  

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