scholarly journals Peptide Mediated Antimicrobial Dental Adhesive System

2019 ◽  
Vol 9 (3) ◽  
pp. 557 ◽  
Author(s):  
Sheng-Xue Xie ◽  
Kyle Boone ◽  
Sarah Kay VanOosten ◽  
Esra Yuca ◽  
Linyong Song ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Bacterial Colonization ◽  
Antibacterial Properties ◽  
Adhesive System ◽  
Dental Composite ◽  
Material Degradation ◽  
Material Weakness ◽  
Food Preservative ◽  
Dental Adhesive ◽  
Adhesive Formulation

The most common cause for dental composite failures is secondary caries due to invasive bacterial colonization of the adhesive/dentin (a/d) interface. Innate material weakness often lead to an insufficient seal between the adhesive and dentin. Consequently, bacterial by-products invade the porous a/d interface leading to material degradation and dental caries. Current approaches to achieve antibacterial properties in these materials continue to raise concerns regarding hypersensitivity and antibiotic resistance. Herein, we have developed a multi-faceted, bio-functionalized approach to overcome the vulnerability of such interfaces. An antimicrobial adhesive formulation was designed using a combination of antimicrobial peptide and a ε-polylysine resin system. Effector molecules boasting innate immunity are brought together with a biopolymer offering a two-fold biomimetic design approach. The selection of ε-polylysine was inspired due to its non-toxic nature and common use as food preservative. Biomolecular characterization and functional activity of our engineered dental adhesive formulation were assessed and the combinatorial formulation demonstrated significant antimicrobial activity against Streptococcus mutans. Our antimicrobial peptide-hydrophilic adhesive hybrid system design offers advanced, biofunctional properties at the critical a/d interface.

scholarly journals Dentin Bonding Agents-II Recent Trials

2012 ◽  
Vol 3 (1) ◽  
pp. 115-118 ◽  
Author(s):  
Surbhi Kakar ◽  
Rohit Nagar
Keyword(s):  
Shear Bond Strength ◽  
Composite Resin ◽  
Antibacterial Properties ◽  
Adhesive System ◽  
Clinical Failure ◽  
Adhesive Systems ◽  
Bonding Agents ◽  
Dental Adhesive ◽  
Dentin Bonding ◽  
Dentin Bonding Agents

ABSTRACT Most modern adhesive systems are superior to their predecessors, especially in terms of retention that is no longer the main cause of premature clinical failure. Recent adhesives also appear less sensitive to substrate and other clinical covariables. Various recent trials have been conducted on various issues like the antibacterial properties of self-ethching dental adhesive system (which are effective against bacteria that invade through microleakage at the resin dentin interphase), shear bond strength of composite resin to dentin, using newer dentin bonding agents, nanoleakege expression in bonded dentin and next generation bonding agents. This paper gives the insight to the various recent trials on dental adhesive systems. How to cite this article Kakar S, Goswami M, Nagar R. Dentin Bonding Agents-II Recent Trials. World J Dent 2012;3(1): 115-118.

scholarly journals Adhesive Antimicrobial Peptides Containing 3,4-Dihydroxy-L-Phenylalanine Residues for Direct One-Step Surface Coating

2021 ◽  
Vol 22 (21) ◽  
pp. 11915
Author(s):  
Young Eun Hwang ◽  
Seonghun Im ◽  
Hyun Kim ◽  
Jung-Hoon Sohn ◽  
Byung-Kwan Cho ◽  
...  
Keyword(s):  
Antimicrobial Peptides ◽  
Antimicrobial Peptide ◽  
Surface Coating ◽  
Bacterial Colonization ◽  
Substrate Surface ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Coating Agent ◽  
C Terminus ◽  
One Step

Bacterial colonization and transmission via surfaces increase the risk of infection. In this study, we design and employ novel adhesive antimicrobial peptides to prevent bacterial contamination of surfaces. Repeats of 3,4-dihydroxy-L-phenylalanine (DOPA) were added to the C-terminus of NKC, a potent synthetic antimicrobial peptide, and the adhesiveness and antibacterial properties of the resulting peptides are evaluated. The peptide is successfully immobilized on polystyrene, titanium, and polydimethylsiloxane surfaces within 10 min in a one-step coating process with no prior surface functionalization. The antibacterial effectiveness of the NKC-DOPA5-coated polystyrene, titanium, and polydimethylsiloxane surfaces is confirmed by complete inhibition of the growth of Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus within 2 h. The stability of the peptide coated on the substrate surface is maintained for 84 days, as confirmed by its bactericidal activity. Additionally, the NKC-DOPA5-coated polystyrene, titanium, and polydimethylsiloxane surfaces show no cytotoxicity toward the human keratinocyte cell line HaCaT. The antimicrobial properties of the peptide-coated surfaces are confirmed in a subcutaneous implantation animal model. The adhesive antimicrobial peptide developed in this study exhibits potential as an antimicrobial surface-coating agent for efficiently killing a broad spectrum of bacteria on contact.

scholarly journals Developmental switch of intestinal antimicrobial peptide expression

2008 ◽  
Vol 205 (1) ◽  
pp. 183-193 ◽  
Author(s):  
Sandrine Ménard ◽  
Valentina Förster ◽  
Michael Lotz ◽  
Dominique Gütle ◽  
Claudia U. Duerr ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
High Performance ◽  
Bacterial Colonization ◽  
Paneth Cell ◽  
Active Form ◽  
Constitutive Expression ◽  
Postnatal Period ◽  
Enteric Infection ◽  
Immune Effector ◽  
Developmental Switch

Paneth cell–derived enteric antimicrobial peptides provide protection from intestinal infection and maintenance of enteric homeostasis. Paneth cells, however, evolve only after the neonatal period, and the antimicrobial mechanisms that protect the newborn intestine are ill defined. Using quantitative reverse transcription–polymerase chain reaction, immunohistology, reverse-phase high-performance liquid chromatography, and mass spectrometry, we analyzed the antimicrobial repertoire in intestinal epithelial cells during postnatal development. Surprisingly, constitutive expression of the cathelin-related antimicrobial peptide (CRAMP) was observed, and the processed, antimicrobially active form was identified in neonatal epithelium. Peptide synthesis was limited to the first two weeks after birth and gradually disappeared with the onset of increased stem cell proliferation and epithelial cell migration along the crypt–villus axis. CRAMP conferred significant protection from intestinal bacterial growth of the newborn enteric pathogen Listeria monocytogenes. Thus, we describe the first example of a complete developmental switch in innate immune effector expression and anatomical distribution. Epithelial CRAMP expression might contribute to bacterial colonization and the establishment of gut homeostasis, and provide protection from enteric infection during the postnatal period.

scholarly journals Engineering Increased Stability in the Antimicrobial Peptide Pediocin PA-1

2000 ◽  
Vol 66 (11) ◽  
pp. 4798-4802 ◽  
Author(s):  
Line Johnsen ◽  
Gunnar Fimland ◽  
Vincent Eijsink ◽  
Jon Nissen-Meyer
Keyword(s):  
Antimicrobial Peptide ◽  
Molecular Mass ◽  
Room Temperature ◽  
Bacteriocin Activity ◽  
Food Preservative ◽  
Food Grade ◽  
First Order ◽  
Methionine Residue ◽  
First Order Kinetics

ABSTRACT Pediocin PA-1 is a food grade antimicrobial peptide that has been used as a food preservative. Upon storage at 4°C or room temperature, pediocin PA-1 looses activity, and there is a concomitant 16-Da increase in the molecular mass. It is shown that the loss of activity follows first-order kinetics and that the instability can be prevented by replacing the single methionine residue (Met31) in pediocin PA-1. Replacing Met by Ala, Ile, or Leu protected the peptide from oxidation and had only minor effects on bacteriocin activity (for most indicator strains 100% activity was maintained). Replacement of Met by Asp was highly deleterious for bacteriocin activity.

scholarly journals RELATION BETWEEN BACTERIAL COLONIZATION IN LUNGS AND LEVELS OF 25-HYDROXYVITAMIN D AND ANTIMICROBIAL PEPTIDE LL-37 IN CHILDREN WITH CYSTIC FIBROSIS

InterConf ◽  
2021 ◽  
pp. 278-282
Author(s):  
Veronika Dudnyk ◽  
Valeriia Demianyshyna
Keyword(s):  
Cystic Fibrosis ◽  
Antimicrobial Peptide ◽  
Bacterial Colonization ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D ◽  
25 Hydroxycholecalciferol

The aim of the study was to assess the bacterial colonization in lungs of children with cystic fibrosis based on the antimicrobial peptide cathelicidin and 25-hydroxycholecalciferol in the serum. Study showed significant correlation between P. aeruginosa infection and cathelicidin and 25-hydroxycholecalciferol levels.

scholarly journals Multilayer Polyelectrolyte Films Functionalized by Insertion of Defensin: a New Approach to Protection of Implants from Bacterial Colonization

2004 ◽  
Vol 48 (10) ◽  
pp. 3662-3669 ◽  
Author(s):  
O. Etienne ◽  
C. Picart ◽  
C. Taddei ◽  
Y. Haikel ◽  
J. L. Dimarcq ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Micrococcus Luteus ◽  
Bacterial Colonization ◽  
Streaming Potential ◽  
Multilayer Films ◽  
Microbial Infection ◽  
Host Protection ◽  
New Strategy ◽  
Local Host ◽  
Positively Charged

ABSTRACT Infection of implanted materials by bacteria constitutes one of the most serious complications following prosthetic surgery. In the present study, we developed a new strategy based on the insertion of an antimicrobial peptide (defensin from Anopheles gambiae mosquitoes) into polyelectrolyte multilayer films built by the alternate deposition of polyanions and polycations. Quartz crystal microbalance and streaming potential measurements were used to follow step by step the construction of the multilayer films and embedding of the defensin within the films. Antimicrobial assays were performed with two strains: Micrococcus luteus (a gram-positive bacterium) and Escherichia coli D22 (a gram-negative bacterium). The inhibition of E. coli D22 growth at the surface of defensin-functionalized films was found to be 98% when 10 antimicrobial peptide layers were inserted in the film architecture. Noticeably, the biofunctionalization could be achieved only when positively charged poly(l-lysine) was the outermost layer of the film. On the basis of the results of bacterial adhesion experiments observed by confocal or electron microscopy, these observations could result from the close interaction of the bacteria with the positively charged ends of the films, which allows defensin to interact with the bacterial membrane structure. These results open new possibilities for the use of such easily built and functionalized architectures onto any type of implantable biomaterial. The modified surfaces are active against microbial infection and represent a novel means of local host protection.

Decreased Bacterial Adhesion to Surface-Treated Titanium

2005 ◽  
Vol 28 (7) ◽  
pp. 718-730 ◽  
Author(s):  
B. Del Curto ◽  
M.F. Brunella ◽  
C. Giordano ◽  
M.P. Pedeferri ◽  
V Valtulina ◽  
...  
Keyword(s):  
Titanium Oxide ◽  
Bacterial Adhesion ◽  
Bacterial Colonization ◽  
Antibacterial Properties ◽  
Bacterial Attachment ◽  
Treatment Results ◽  
Bone Implant ◽  
Crystalline Layer ◽  
Progressive Loss ◽  
Modification Treatment

Osteointegrative dental implants are widely used in implantology for their well-known excellent performance once implanted in the host. Remarkable bacterial colonization along the transgingival region may result in a progressive loss of adhesion at gum-implant interface and an increase of the bone area exposed to pathogens. This phenomenon may negatively effect the osteointegration process and cause, in the most severe cases, implant failure. The presence of bacteria at implant site affect the growth of new bone tissue and consequently, the achievement of a mechanically stable bone-implant interface, key parameters for a suitable implant osteointegration. In the present work, a novel surface treatment has been developed and optimized in order to convert the amorphous titanium oxide in a crystalline layer enriched in anatase capable of providing not only antibacterial properties but also of stimulating the precipitation of apatite when placed in simulated body fluid. The collected data have shown that the tested treatment results in a crystalline anatase-type titanium oxide layer able to provide a remarkable decrease in bacterial attachment without negatively effecting cell metabolic activity. In conclusion, the surface modification treatment analyzed in the present study might be an elegant way to reduce the risk of bacterial adhesion and increase the lifetime of the transgingival component in the osteointegrated dental implant.

scholarly journals Evaluation of the Physicochemical and Antibacterial Properties of Experimental Adhesives Doped with Lithium Niobate

Polymers ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1330 ◽  
Author(s):  
Laisa Cruzetta ◽  
Isadora M. Garcia ◽  
Gabriela de Souza Balbinot ◽  
Amanda S. Motta ◽  
Fabrício M. Collares ◽  
...  
Keyword(s):  
Statistical Difference ◽  
Antibacterial Properties ◽  
Control Group ◽  
Microtensile Bond Strength ◽  
Adhesive Resin ◽  
Dental Adhesives ◽  
Dental Adhesive ◽  
Negative Effect ◽  
Methacrylate Monomers

The aim of the present study was to formulate dental adhesives with different concentrations of LiNbO3 and to evaluate their physicochemical and antibacterial properties. A dental adhesive was formulated using methacrylate monomers and photoinitiators and used as a control filler-free group. Subsequently, three experimental adhesives doped with LiNbO3 at different concentrations (1 wt.%, 2 wt.%, and 5 wt.%) were also formulated. All the experimental adhesives were assessed to evaluate the degree of conversion (DC), softening in solvent, immediate and long-term microtensile bond-strength (μ-TBS), radiopacity, ultimate tensile strength, and antibacterial activity. The incorporation of 1 wt.% of LiNbO3 had no negative effect on the DC of the adhesive resin compared to the control group (p > 0.05). We observed a decrease in the percentage of softening in solvent in the group LiNbO3 at 1 wt.% (p < 0.05). The addition of LiNbO3 increased the radiopacity at a concentration above 2 wt.%, and there was also an increase in cohesive strength (p < 0.05). The immediate μ-TBS increased for LiNbO3 at 5 wt.% (p < 0.05), and there was no statistical difference for the other groups compared to the control (p > 0.05). After six months, the group with 5 wt.% still presented the highest μ-TBS (p < 0.05). The adhesives showed no antimicrobial activity (p > 0.05). LiNbO3 was successfully incorporated in dental adhesives, increasing the radiopacity and their resistance to degradation. Although LiNbO3 offered no antibacterial properties, the reliability of LiNbO3 incorporation in the adhesive encourages new tests to better investigate the antimicrobial action of LiNbO3 through temperature variation.

scholarly journals Microencapsulation of Thymol in Poly(lactide-co-glycolide) (PLGA): Physical and Antibacterial Properties

Materials ◽  
2019 ◽  
Vol 12 (7) ◽  
pp. 1133 ◽  
Author(s):  
Zhu Zhu ◽  
Tiantian Min ◽  
Xueji Zhang ◽  
Yongqiang Wen
Keyword(s):  
Storage Stability ◽  
In Vitro Release ◽  
Antibacterial Properties ◽  
Spherical Shape ◽  
Food Preservative ◽  
And Storage ◽  
Fast Release ◽  
Vitro Release ◽  
And Staphylococcus Aureus

Thymol has been shown to be a safe and effective broad-spectrum antimicrobial agent that can be used as a food preservative. However, its volatile characteristics and strong odor limit its use in food products. The microencapsulation of this essential oil in biopolymers could overcome these disadvantages. In this work, thymol-loaded poly(lactide-co-glycolide) (PLGA) microparticles were successfully prepared and the optimal encapsulation efficiency was obtained at 20% (w/w) thymol. Microparticles containing thymol presented a spherical shape and smooth surface. Microencapsulation significantly improved the thermal and storage stability of thymol. In vitro release profiles demonstrated an initial fast release followed by a slow and sustained release. Thymol-loaded microparticles had strong antibacterial activity against Escherichia coli and Staphylococcus aureus, and the effectiveness of their antibacterial properties was confirmed in a milk test. Therefore, the thymol-loaded microparticles show great potential for use as an antimicrobial and as preservation additives in food.

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