scholarly journals Nanomaterials in Craniofacial Tissue Regeneration: A Review

2019 ◽  
Vol 9 (2) ◽  
pp. 317 ◽  
Author(s):  
Owen Tao ◽  
David Wu ◽  
Hieu Pham ◽  
Neelakshi Pandey ◽  
Simon Tran
Keyword(s):  
Extracellular Matrix ◽  
Temporomandibular Joint ◽  
Dental Implants ◽  
Tissue Regeneration ◽  
Mineral Trioxide Aggregate ◽  
Tissue Growth ◽  
Bone Tissue Regeneration ◽  
Scaffold Materials ◽  
Craniofacial Defects ◽  
Craniofacial Tissue

Nanotechnology is an exciting and innovative field when combined with tissue engineering, as it offers greater versatility in scaffold design for promoting cell adhesion, proliferation, and differentiation. The use of nanomaterials in craniofacial tissue regeneration is a newly developing field that holds great potential for treating craniofacial defects. This review presents an overview of the nanomaterials used for craniofacial tissue regeneration as well as their clinical applications for periodontal, vascular (endodontics), cartilage (temporomandibular joint), and bone tissue regeneration (dental implants and mandibular defects). To enhance periodontal tissue regeneration, nanohydroxyapatite was used in conjunction with other scaffold materials, such as polylactic acid, poly (lactic-co-glycolic acid), polyamide, chitosan, and polycaprolactone. To facilitate pulp regeneration along with the revascularization of the periapical tissue, polymeric nanofibers were used to simulate extracellular matrix formation. For temporomandibular joint (cartilage) engineering, nanofibrous-type and nanocomposite-based scaffolds improved tissue growth, cell differentiation, adhesion, and synthesis of cartilaginous extracellular matrix. To enhance bone regeneration for dental implants and mandibular bone defects, nanomaterials such as nanohydroxyapatite composite scaffolds, nanomodified mineral trioxide aggregate, and graphene were tested. Although the scientific knowledge in nanomaterials is rapidly advancing, there remain many unexplored data regarding their standardization, safety, and interactions with the nanoenvironment.

scholarly journals Nanoscale Strontium-Substituted Hydroxyapatite Pastes and Gels for Bone Tissue Regeneration

Nanomaterials ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1611
Author(s):  
Caroline J. Harrison ◽  
Paul V. Hatton ◽  
Piergiorgio Gentile ◽  
Cheryl A. Miller
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Culture Media ◽  
Full Range ◽  
Sol Gel ◽  
Tissue Growth ◽  
Bone Tissue Regeneration ◽  
Tissue Culture Media ◽  
Bone Deposition

Injectable nanoscale hydroxyapatite (nHA) systems are highly promising biomaterials to address clinical needs in bone tissue regeneration, due to their excellent biocompatibility, bioinspired nature, and ability to be delivered in a minimally invasive manner. Bulk strontium-substituted hydroxyapatite (SrHA) is reported to encourage bone tissue growth by stimulating bone deposition and reducing bone resorption, but there are no detailed reports describing the preparation of a systematic substitution up to 100% at the nanoscale. The aim of this work was therefore to fabricate systematic series (0–100 atomic% Sr) of SrHA pastes and gels using two different rapid-mixing methodological approaches, wet precipitation and sol-gel. The full range of nanoscale SrHA materials were successfully prepared using both methods, with a measured substitution very close to the calculated amounts. As anticipated, the SrHA samples showed increased radiopacity, a beneficial property to aid in vivo or clinical monitoring of the material in situ over time. For indirect methods, the greatest cell viabilities were observed for the 100% substituted SrHA paste and gel, while direct viability results were most likely influenced by material disaggregation in the tissue culture media. It was concluded that nanoscale SrHAs were superior biomaterials for applications in bone surgery, due to increased radiopacity and improved biocompatibility.

Ornamenting 3D printed scaffolds with cell-laid extracellular matrix for bone tissue regeneration

Biomaterials ◽  
2015 ◽  
Vol 37 ◽  
pp. 230-241 ◽  
Author(s):  
Falguni Pati ◽  
Tae-Ha Song ◽  
Girdhari Rijal ◽  
Jinah Jang ◽  
Sung Won Kim ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Bone Tissue Regeneration ◽  
3D Printed

scholarly journals Extracellular matrix compression temporally regulates microvascular angiogenesis

2020 ◽  
Vol 6 (34) ◽  
pp. eabb6351 ◽  
Author(s):  
M. A. Ruehle ◽  
E. A. Eastburn ◽  
S. A. LaBelle ◽  
L. Krishnan ◽  
J. A. Weiss ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Signaling Pathways ◽  
Tissue Regeneration ◽  
Network Formation ◽  
Bone Tissue Regeneration ◽  
Dynamic Matrix ◽  
Matrix Mechanics ◽  
Matrix Compression ◽  
Vessel Networks

Mechanical cues influence tissue regeneration, and although vasculature is known to be mechanically sensitive, little is known about the effects of bulk extracellular matrix deformation on the nascent vessel networks found in healing tissues. Previously, we found that dynamic matrix compression in vivo potently regulated revascularization during bone tissue regeneration; however, whether matrix deformations directly regulate angiogenesis remained unknown. Here, we demonstrated that load initiation time, magnitude, and mode all regulate microvascular growth, as well as upstream angiogenic and mechanotransduction signaling pathways. Immediate load initiation inhibited angiogenesis and expression of early sprout tip cell selection genes, while delayed loading enhanced microvascular network formation and upstream signaling pathways. This research provides foundational understanding of how extracellular matrix mechanics regulate angiogenesis and has critical implications for clinical translation of new regenerative medicine therapies and physical rehabilitation strategies designed to enhance revascularization during tissue regeneration.

scholarly journals Bioink Formulations for Bone Tissue Regeneration

2021 ◽  
Vol 9 ◽  
Author(s):  
Na Li ◽  
Rui Guo ◽  
Zhenyu Jason Zhang
Keyword(s):  
Extracellular Matrix ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Three Dimensional ◽  
Bone Tissue Regeneration ◽  
3D Bioprinting ◽  
Native Bone ◽  
Technical Requirements ◽  
Tissue Scaffolding ◽  
Functional Transformations

Unlike the conventional techniques used to construct a tissue scaffolding, three-dimensional (3D) bioprinting technology enables fabrication of a porous structure with complex and diverse geometries, which facilitate evenly distributed cells and orderly release of signal factors. To date, a range of cell-laden materials, such as natural or synthetic polymers, have been deployed by the 3D bioprinting technique to construct the scaffolding systems and regenerate substitutes for the natural extracellular matrix (ECM). Four-dimensional (4D) bioprinting technology has attracted much attention lately because it aims to accommodate the dynamic structural and functional transformations of scaffolds. However, there remain challenges to meet the technical requirements in terms of suitable processability of the bioink formulations, desired mechanical properties of the hydrogel implants, and cell-guided functionality of the biomaterials. Recent bioprinting techniques are reviewed in this article, discussing strategies for hydrogel-based bioinks to mimic native bone tissue-like extracellular matrix environment, including properties of bioink formulations required for bioprinting, structure requirements, and preparation of tough hydrogel scaffolds. Stimulus mechanisms that are commonly used to trigger the dynamic structural and functional transformations of the scaffold are analyzed. At the end, we highlighted the current challenges and possible future avenues of smart hydrogel-based bioink/scaffolds for bone tissue regeneration.

scholarly journals Osteogenic extracellular matrix sheet for bone tissue regeneration

2018 ◽  
Vol 36 ◽  
pp. 69-80 ◽  
Author(s):  
T Onishi ◽  
◽  
T Shimizu ◽  
M Akahane ◽  
S Omokawa ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Bone Tissue Regeneration

Apicoectomy and Enucleation on Maxillary Lateral Incisor with Radicular Cyst

2020 ◽  
Vol 48 ◽  
pp. 92-98
Author(s):  
Fitriana Hesti Dian ◽  
Yulita Kristanti ◽  
Wignyo Hadriyanto
Keyword(s):  
Bone Graft ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Mineral Trioxide Aggregate ◽  
Bone Tissue Regeneration ◽  
Maxillary Incisor ◽  
Radicular Cyst ◽  
The Third ◽  
History Of ◽  
Maxillary Lateral Incisor

Introduction. A radicular cyst is an odontogenic cyst originated from residual epithelial cells (remnants of Malassez cells) of periodontal ligaments with the previous history of chronic inflammation. The radicular cyst usually results from traumatized pulp or necrotic pulp that is left untreated. This study aimed to treat the maxillary lateral incisor with necrotic pulp and radicular cyst by apicoectomy, retrograde closure using mineral trioxide aggregate, followed by enucleation and bone graft application. Case Report. A 28-year-old female patient who suffered from recurrence pain and swelling in the right maxillary incisor area came to Gadjah Mada University Dental. The patient had a history of trauma on her maxillary right incisor 12 years ago. Vitality test, percussion test and palpation test showed negative results. Cone beam computed tomography radiograph showed circular radiolucent lesions with a firm radiopaque border surrounding the apical of tooth resulted in a cortical bone loss on the labial aspect. The lateral maxillary incisor was diagnosed as necrotic pulp with the radicular cyst. Conventional endodontic treatment was performed in two visits using calcium hydroxide as intracanal medicament followed by class I composite resin restoration. Apicoectomy procedure was performed on the third visit using mineral trioxide aggregate as retrograde filling material followed by enucleation of radicular cysts and application of bone graft as bone regeneration material. Three months of control after treatment showed good bone tissue regeneration. Post-operative control in the third month showed functional bone tissue regeneration. Conclusion. Apicoectomy and retrograde closure using aggregate mineral trioxide followed by enucleation and bone graft application is a treatment of choice for pulp necrosis with the radicular cyst.

Prospects for the use of collagen-containing matrices in directed tissue regeneration. Literature review

2021 ◽  
pp. 9-13
Author(s):  
Е. М. Boyko ◽  
A. A. Dolgalev ◽  
D. V. Stomatov ◽  
S. G. Ivashkevih ◽  
A. A. Chagarov ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Connective Tissue ◽  
Tissue Regeneration ◽  
San Antonio ◽  
Feedback Mechanism ◽  
Tissue Growth ◽  
Negative Feedback Mechanism ◽  
Decay Products ◽  
The Relationship

Studies of recent decades have convincingly shown that collagen in connective tissue plays not only a structural role. In the 80s of the XX centu[1]ry, A. Pishinger and H. Heine suggested the informative-regulatory role of collagen in the extracellular matrix (A. Pischinger, 1990). In recent years, the morphogenetic function of collagen has been actively studied, the implementation of which is possible due to the presence of collagen re[1]ceptors on the surface of various cell populations, such as platelets and fibroblasts. Collagen regulates the remodeling of the extracellular matrix (J. D. San Antonio et al., 2020). At the same time, its decay products, which stimulate growth by the negative feedback mechanism, are probably of great importance. In general, the relationship between the synthesis and breakdown of collagen is of fundamental importance for the regulation of connective tissue growth.

Monitoring of Extracellular Matrix Protein Conformations in the Presence of Biomimetic Bone Tissue Regeneration Scaffolds

2020 ◽  
Vol 865 ◽  
pp. 43-47
Author(s):  
Rodriguez Barroso ◽  
Lanzagorta Garcia ◽  
Farah Alwani Azaman ◽  
Declan M. Devine ◽  
Mark Lynch ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Matrix Protein ◽  
Tissue Scaffolds ◽  
C2c12 Cells ◽  
Bone Tissue Regeneration ◽  
Extracellular Matrix Protein ◽  
C2c12 Myoblast ◽  
Triangular Silver Nanoplates

Tissue scaffolds can be designed to mimic the native extracellular matrix (ECM), making them attractive for the development for a range of regenerative medicine applications. The macromolecules present in the ECM are critical for the provision of structural support to surrounding cells and signalling cues for the modulation of diverse processes including cell migration, proliferation and healing activation. Here, conformational and transitional behaviour of the ubiquitous ECM protein, fibronectin (Fn), in the presence of bone tissue regeneration scaffolds and living C2C12 myoblast cells is reported. Spectral monitoring of Fn functionalised high plasmonic resonance responsive gold-edge-coated triangular silver nanoplates (AuTSNP) is used to distinguish between compact and extended fibronectin conformations. Large spectral red shifts of ~20 to ~59 nm indicate Fn unfolding and fibril formation on incubation with C2C12 cells. The label-free nature, excellent sensitivity and straightforward application of the AuTSNP within cellular environments presents them as a powerful new tool to signature protein conformational activity in living cells and monitor essential protein activity for the assisted development of improved tissue scaffolds promoting enhanced tissue repair.

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