scholarly journals Antibacterial Efficacy of Commercially Available Essential Oils Tested Against Drug-Resistant Gram-Positive Pathogens

2018 ◽  
Vol 8 (11) ◽  
pp. 2201 ◽  
Author(s):  
Hercules Sakkas ◽  
Vangelis Economou ◽  
Panagiota Gousia ◽  
Petros Bozidis ◽  
Vasilios Sakkas ◽  
...  
Keyword(s):  
Essential Oils ◽  
Ocimum Basilicum ◽  
Thymus Vulgaris ◽  
Matricaria Chamomilla ◽  
Bacterial Strains ◽  
Melaleuca Alternifolia ◽  
Gram Positive ◽  
Tea Tree ◽  
Thymus Capitatus ◽  
Basil Oil

The potential antibacterial activity of basil (Ocimum basilicum), chamomile (Matricaria chamomilla), origanum (Thymus capitatus), tea tree (Melaleuca alternifolia) and thyme (Thymus vulgaris) essential oils, was investigated against 29 Gram-positive bacterial strains isolated from wastewater treatment plants, clinical samples (n = 25) and American Type Culture Collection (ATCC) reference strains (n = 4). Wild bacterial strains included methicillin-resistant Staphylococcus aureus (n = 16) and vancomycin-resistant Enterococcus spp. (n = 9). The antimicrobial activity of the selected oils was studied using the broth macrodilution method. The Minimal Inhibitory Concentration (MIC) values for S. aureus ranged from 0.06 to 0.5% (v/v) for origanum oil, 0.06 to 1% (v/v) for thyme oil, 0.12 to 1% (v/v) for tea tree oil, 0.25 to 4% (v/v) for basil oil and 2 to >4% (v/v) for chamomile oil. For enterococci the MIC values were significantly higher ranging from 0.25 to 1% (v/v), 0.5 to 2% (v/v), 1 to 4% (v/v), 4 to >4% (v/v) and >4% (v/v) for the above-mentioned oils, respectively. The main compounds of the tested essential oils were: estragole (Ocimum basilicum), bisabolol and trans-b-farnesene (Matricaria chamomilla), carvacrol and thymol (Thymus capitatus), terpinen-4-ol and p-cymene (Melaleuca alternifolia), thymol, linalool, and p-cymene (Thymus vulgaris). Origanum essential oil yielded the best antimicrobial results followed by thyme, tea tree, and basil oil, while chamomile oil exhibited weak antibacterial properties.

scholarly journals Essential Oils in the Control of Anthracnose on ‘Prata Ana’ Banana

2018 ◽  
Vol 10 (9) ◽  
pp. 116
Author(s):  
M. L. M. Rodrigues ◽  
E. H. Mizobutsi ◽  
I. R. F. F. Fagundes ◽  
M. B. Fernandes ◽  
G. P. Mizobutsi ◽  
...  
Keyword(s):  
Essential Oils ◽  
Mycelial Growth ◽  
Fungal Growth ◽  
Zingiber Officinale ◽  
Thymus Vulgaris ◽  
Tea Tree Oil ◽  
Melaleuca Alternifolia ◽  
Negative Effects ◽  
Tea Tree ◽  
Colletotrichum Musae

Alternative forms of disease control in fruits have been researched in an attempt to minimize the negative effects of chemical control on men and environment. The aim of this study was to determine the best concentration of essential oils to control the development of Colletotrichum musae and anthracnose intensity in ‘Prata Anã’ banana. In order to inhibit germination, mycelial growth and sporulation, four essential oils of clove (Eugenia caryophyllus), tea tree (Melaleuca alternifolia), thyme (Thymus vulgaris) and ginger (Zingiber officinale) species were used at concentrations of 2, 4, 6 and 8 μL. In fruits, the same essential oils were applied at concentrations of 80, 160, 240 and 320 μL to evaluate the incidence and severity of anthracnose. Clove and thyme oils were the most efficient in inhibiting mycelial growth, germination and sporulation at all concentrations tested. The volatile compounds present in clove and thyme oils provide complete inhibition of fungal growth. Tea tree and ginger oils have the ability to suppress anthracnose in ‘Prata Anã’ banana. Concentrations of 160 μL of ginger oil, 160 and 240 μL of tea tree oil reduced the anthracnose severity in fruits. Ginger and tea tree essential oils have the potential to control banana anthracnose, reducing the anthracnose incidence in fruits by 48% and 24%, respectively.

scholarly journals Chemical Composition, Olfactory Analysis and Antibacterial Activity of Thymus vulgaris Chemotypes Geraniol, 4-Thujanol/Terpinen-4-ol, Thymol and Linalool Cultivated in Southern France

2012 ◽  
Vol 7 (8) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Erich Schmidt ◽  
Jürgen Wanner ◽  
Martina Höferl ◽  
Leopold Jirovetz ◽  
Gerhard Buchbauer ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Thymus Vulgaris ◽  
Gram Positive ◽  
Southern France ◽  
Food Borne ◽  
Brochothrix Thermosphacta ◽  
Olfactory Analysis ◽  
The Impact ◽  
And Staphylococcus Aureus

The essential oils of four chemotypes of Thymus vulgaris L. (Lamiaceae) were analyzed for their composition and antibacterial activity to assess their different properties. GC-MS and GC-FID analyses revealed that the essentials oils can be classified into the chemotypes thymol (41.0% thymol), geraniol (26.4% geraniol), linalool (72.5% linalool) and 4-thujanol/terpinen-4-ol (42.2% cis- and 7.3% trans-sabinene hydrate, 6.5 % terpinen-4-ol). The olfactory examination confirmed the explicit differences between these chemotypes. Furthermore, antibacterial activity was investigated against several strains of two Gram-positive ( Brochothrix thermosphacta and Staphylococcus aureus) and four Gram-negative food-borne bacteria ( Escherichia coli, Salmonella abony, Pseudomonas aeruginosa and P. fragi). All essential oil samples were demonstrated to be highly effective against Gram-positive strains, whereas the impact on Gramnegative microorganisms was significantly smaller, but still considerable. The results obtained indicate that, despite their different properties, the essential oils of selected T. vulgaris chemotypes are potent antimicrobials to be employed as useful additives in food products as well as for therapeutic applications.

scholarly journals In Vitro Efficacy of Essential Oils from Melaleuca Alternifolia and Rosmarinus Officinalis, Manuka Honey-based Gel, and Propolis as Antibacterial Agents Against Canine Staphylococcus Pseudintermedius Strains

Antibiotics ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 344
Author(s):  
Gabriele Meroni ◽  
Elena Cardin ◽  
Charlotte Rendina ◽  
Valentina Rafaela Herrera Millar ◽  
Joel Fernando Soares Filipe ◽  
...  
Keyword(s):  
Essential Oils ◽  
Antimicrobial Agents ◽  
Antibacterial Properties ◽  
Rosmarinus Officinalis ◽  
Diffusion Method ◽  
Melaleuca Alternifolia ◽  
Disk Diffusion Method ◽  
Staphylococcus Pseudintermedius ◽  
Tea Tree

Essential oils (EOs) and honeybee products (e.g., honey and propolis) are natural mixtures of different volatile compounds that are frequently used in traditional medicine and for pathogen eradication. The aim of this study was to evaluate the antibacterial properties of tea tree (Melaleuca alternifolia) EO (TTEO), Rosmarinus officinalis EO (ROEO), manuka-based gel, and propolis against 23 strains of Staphylococcus pseudintermedius (SP) isolated from canine pyoderma. Antimicrobial resistance screening was assessed using a panel of nine antimicrobial agents coupled with a PCR approach. An aromatogram was done for both EOs, using the disk diffusion method. The minimum inhibitory concentration (MIC) was determined for all the compounds. Among the 23 SP strains, 14 (60.9%) were multidrug-resistant (MDR), 11 strains (47.8%) were methicillin-resistant (MRSP), and 9 (39.1%) were non-MDR. The mean diameter of the inhibition zone for Melaleuca and Rosmarinus were 24.5 ± 8.8 mm and 15.2 ± 8.9 mm, respectively, resulting as statistically different (p = 0.0006). MIC values of TTEO and ROEO were similar (7.6 ± 3.2% and 8.9 ± 2.1%, respectively) and no statistical significances were found. Honeybee products showed lower MIC compared to those of EOs, 0.22 ± 0.1% for Manuka and 0.8 ± 0.5% for propolis. These findings reveal a significant antibacterial effect for all the tested products.

scholarly journals ANTIMICROBIAL AND SYNERGISTIC ACTIVITY OF ESSENTIAL OILS FACING ISOLATED BACTERIA FROM SURGICAL STAFF’S

2019 ◽  
Vol 5 ◽  
Author(s):  
Tatiana Areas Cruz ◽  
Fernanda Rodrigues Torres ◽  
Monise Fazolin Petrucelli ◽  
Mariana Heinzen De Abreu ◽  
Silvia Sidneia Silva ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Staphylococcus Epidermidis ◽  
Citrus Sinensis ◽  
Concentration Index ◽  
Inhibitory Concentration ◽  
Thymus Vulgaris ◽  
Synergistic Activity ◽  
Bacterial Strains ◽  
Microdilution Method

Objective:to evaluate antimicrobial and synergistic activity of essential oils (Citrus aurantifolia, Citrus sinensis, Mentha viridisand Thymus vulgaris) against isolated bacteria from surgical staff’s hands and bacterial strains. Method:the antimicrobial activity of oils was analyzed by microdilution method to determine the Minimum Inhibitory Concentration. Moreover, the combinatory effect of the oil that presented greater effectiveness using gentamycin sulphate through the Fractional Inhibitory Concentration index was tested. The study was performed over the period 2016-2018. Results:T. vulgaris was effective against Staphylococcus saprophyticus with a concentration of 0.0008 µL.mL-1and against the ATCC standard strains of Staphylococcus aureus, Salmonella choleraesuis,Staphylococcus epidermidis and Proteus vulgarisand the bacteria S. aureus, S. epidermidisand Bacillus sp. with MIC ≥ 0.10 µL.mL-1. This oil association with gentamycin sulphate showed synergistic activity againstS. epidermidis. Conclusion:T. vulgarisessential oil showed expressive antimicrobial activity against ATCC and isolated clinic microorganisms, suggesting broad spectrum of activity.

scholarly journals Toxicity and Biochemical Effects of Cumin and Basil Essential Oils on Tribolium Castaneum

2021 ◽  
Vol 52 (3) ◽  
pp. 39-48
Author(s):  
A.F. Omar ◽  
M.E. El-Ebiary ◽  
G.M. Nasr ◽  
H.M. Hassan
Keyword(s):  
Mass Spectrometry ◽  
Alkaline Phosphatase ◽  
Essential Oils ◽  
Tribolium Castaneum ◽  
Amylase Activity ◽  
Ocimum Basilicum ◽  
Red Flour Beetle ◽  
Whole Body ◽  
Biochemical Effects ◽  
Basil Oil

Abstract The essential oils (EOs) of cumin (Cuminum cyminum L.) seeds and basil (Ocimum basilicum L.) herb were extracted by hydrodistillation and tested against the red flour beetle, Tribolium castaneum (Herbst) for insecticidal and biochemical effects on certain enzymes of this insect. Major components of C. cyminum EO determined by gass chromatography-mass spectrometry (GC/MS) analysis were γ-terpinene (15.78 %) and benzenemethanol (11.32 %), while those of O. basilicum EO were linalool (56.7 %) and epi-α-cadinol (11.4 %). The lethal concentration values for 50% mortality after three days of T. castaneum whole body exposure were 678 mg kg–1 for cumin oil and 755 mg kg–1 for basil oil. The enzymatic activity of treated insects showed a reduction in total protein, alkaline phosphatase, aspartate aminotransferase, and alanine aminotransferase compared to untreated ones. However, α-amylase activity increased with both tested EOs. Hence, for T. castaneum control, these EOs may represent alternatives to conventional insecticides.

scholarly journals Purple basil (Ocimum basilicum var. purple) oil under drought stress effects on Aspergillus fumigates fungus in the food industry

2021 ◽  
Author(s):  
Arghavan Haji Mohammad ◽  
Hesam Sharifi Nejad
Keyword(s):  
Drought Stress ◽  
Essential Oil ◽  
Essential Oils ◽  
Food Industry ◽  
Field Capacity ◽  
Ocimum Basilicum ◽  
Research Station ◽  
Minimum Concentration ◽  
Aspergillus Fumigates ◽  
Basil Oil

Abstract To investigate the effect of drought stress Purple Basil (Ocimum basilicum var.purple), on Aspergillus fumigates in food industry, an experiment was performed in the Alborz Karaj research station, and extracted essential oils of purple basil in Agricultural Education Center of Imam Khomeini in 2011, wasperformed. Purple basil essential oil obtained from the stress levels of 30%, 60% and 90% field capacity (FC) is. Using brows microdilution, the average minimum inhibitory concentration and the mean minimum concentration of essential oils produced in concentrations of 0/25, 0/5, 1, 1/5, 2 and 2/5 mg/ml against fungi Aspergillus fumigates was determined in three replicates. The results showed that under conditions of purple basil oil 30% field capacity had the strongest activity against the fungi studied (0/37 mg/ml = 90MIC).Antibacterial activity of essential oil and the lowest was seen in 90% of field capacity (0/97mg/ml = 90MIC). Purple basil oil under the terms of the MFC as the lowest 30% of field capacity (0/56 mg/ml) and maximum (0/99 mg/ml) was seen in 90% of the crop. According to survey results in the absence of purple basil oil 30% field capacity (severe water stress) was a good performance against fungi Aspergillus fumigates antifungal and take advantage of it to use its influence in the food industry, pharmaceutical and health is .

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