scholarly journals The Construction of an Engineered Bacterial Strain and Its Application in Accumulating Mercury from Wastewater

2018 ◽  
Vol 8 (9) ◽  
pp. 1572 ◽  
Author(s):  
Shenghe Chang ◽  
Zhengjing Wu ◽  
Wei Sun ◽  
Haiyan Shu
Keyword(s):  
Mercuric Chloride ◽  
Bacterial Strain ◽  
Inorganic Mercury ◽  
Luria Bertani ◽  
Methylmercury Chloride ◽  
Ph Values ◽  
Minimum Inhibitory Concentrations ◽  
Strain Bl21 ◽  
Engineered Bacteria

To remove organic and inorganic mercury from wastewater, an engineered bacterial strain, BL21-7, was constructed that contained the artificial operon P16S-g10-merT-merP-merB1-merB2-ppk-rpsT. For BL21-7, the minimum inhibitory concentrations of mercuric chloride, methylmercury chloride and phenylmercury chloride in Luria-Bertani (LB) medium were 100 µmol/L, 60 µmol/L and 80 µmol/L, respectively. After being cultured in three media (liquid LB containing 80 µmol/L mercuric chloride, 40 µmol/L methylmercury chloride or 60 µmol/L phenylmercury chloride) for 72 h, the engineered bacteria accumulated up to 70.5 ± 1.5 µmol/L, 33.5 ± 3.2 µmol/L and 45.3 ± 3.7 µmol/L of mercury, respectively. In the presence of 10 µmol/L Cd2+, 10 µmol/L Pb2+ or 10 µmol/L Cu2+, the accumulation of mercurial derivatives by BL21-7 was not affected. BL21-7 could accumulate mercury well in media with pH values ranging from 5 to 8 and it could work well at temperatures from 25 °C to 37 °C. After BL21-7 was added to wastewater and cultured for 24 h, approximately 43.7% of the Hg in the wastewater was removed.

CHEMICAL CONTROL OF SNOW MOULD OF GRASSES AND ALFALFA IN ALBERTA

1961 ◽  
Vol 41 (4) ◽  
pp. 744-750 ◽  
Author(s):  
J. B. Lebeau ◽  
M. W. Cormack ◽  
E. W. B. Ward
Keyword(s):  
Mercuric Chloride ◽  
Chemical Control ◽  
Inorganic Mercury ◽  
Sodium Tetraborate ◽  
Turf Grass ◽  
Mercurous Chloride ◽  
Margin Of Safety ◽  
Mercury Salts ◽  
Early Fall ◽  
Snow Mould

Chemical tests for control of snow mould of turf grass and alfalfa were conducted at Edmonton from 1945 to 1957. Of a number of chemicals tested, inorganic mercury salts provided the best control. Mercuric chloride was more effective than mercurous chloride for treating alfalfa but no difference in control was observed from the two chemicals on turf grass. Sodium tetraborate sprays applied to the foliage of alfalfa in early fall gave remarkable control of the disease but are not recommended because of the low margin of safety from boron injury.

scholarly journals The Effects of Chronic Ingestion of Mercuric Chloride on Fertility and Testosterone Levels in Male Sprague Dawley Rats

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
John C. Heath ◽  
Y. Abdelmageed ◽  
Tim D. Braden ◽  
Hari O. Goyal
Keyword(s):  
Mercuric Chloride ◽  
Inorganic Mercury ◽  
Male Reproduction ◽  
Male Rats ◽  
Male Rat ◽  
Sprague Dawley ◽  
Testosterone Levels ◽  
Mercury Compounds ◽  
Sprague Dawley Rats ◽  
Sperm Counts

Although male infertility is well researched, the effects of inorganic mercury on male reproduction and fertility are less well known. Studies pertaining to mercury and male fertility identified reduced concentration of testosterone in the serum of male workers, a toxic influence on fertility of organic mercury compounds within concentrations at the workplace, and increased days to pregnancy. We evaluated the effect of chronic mercuric chloride (HgCl2) exposure in male rats on reproductive endpoints. Thirty-day old male Sprague Dawley rats (n=31) were exposed to 0.0, 1.0, or 2.0 mg/kg/day of HgCl2via gavage. After 60 days exposure, they were housed with nonexposed females for 21 days. A survivor analysis revealed the exposed animals took longer to impregnate the females and had a lower rate of impregnation. Further statistical analysis revealed a lower correlation between testicular testosterone levels and days to impregnate, and also lower sperm counts in the epididymis head and body of the exposed males. The results indicate that HgCl2exposure had significant adverse effects on male rat reproduction endpoints including fertility at a dose that was not clinically toxic.

scholarly journals A highly efficient auxin-producing bacterial strain and its effect on plant growth

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Seunghye Park ◽  
A-Leum Kim ◽  
Yoon-Kyung Hong ◽  
Ji-Hwan Shin ◽  
Se-Hwan Joo
Keyword(s):  
Plant Growth ◽  
Root Growth ◽  
Bacterial Strain ◽  
High Efficiency ◽  
Early Stage ◽  
Plant Root ◽  
Luria Bertani ◽  
Process Waste ◽  
Luria Bertani Broth ◽  
Rdna Sequencing

Abstract Background Various bacteria promote plant root growth in the rhizosphere, as a measure of securing and enlarging their ecological niche. These interactions are mediated by plant growth regulators (PGRs) such as auxin, and indole-3-acetic acid (IAA) is one of the physiologically active auxin. In this study, we isolated an unusual bacterial strain from food process waste with high efficiency and demonstrated its effects on plant rooting and early-stage growth. Results The efficiency of this bacterial strain in producing IAA was 16.6 mg/L/h in Luria-Bertani broth containing 0.05% l-tryptophan (Trp) at room temperature (24 ± 2 °C). Its IAA production was highly dependent on the presence of precursor, Trp. This bacterium was identified as Ignatzschineria sp. by 16S rDNA sequencing. Its bacterial culture supernatant (BCS) enhanced plant root initiation, root growth, and plant growth in the early stages. The root mass formed BCS-treated in apple mint cuttings was twofold of that formed in the control. The root number and length were 46% and 18% higher, respectively, in BCS-treated chrysanthemum cuttings than in the control. Conclusions These results show that the BCS of Ignatzschineria sp. CG20001 isolate obtained in this study can be used for agricultural applications. In addition, the novelty of this strain makes it a valuable genetic resource for biotechnological applications.

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