scholarly journals Organic Electrochemical Transistor Microplate for Real-Time Cell Culture Monitoring

2017 ◽  
Vol 7 (10) ◽  
pp. 998 ◽  
Author(s):  
Ota Salyk ◽  
Jan Víteček ◽  
Lukáš Omasta ◽  
Eva Šafaříková ◽  
Stanislav Stříteský ◽  
...  
Keyword(s):  
Cell Culture ◽  
Real Time ◽  
Electrochemical Transistor ◽  
Culture Monitoring

Impedance-based drug-resistance characterization of colon cancer cells through real-time cell culture monitoring

Talanta ◽  
2021 ◽  
Vol 222 ◽  
pp. 121441
Author(s):  
Fuentes-Vélez Susana ◽  
Fagoonee Sharmila ◽  
Sanginario Alessandro ◽  
Gallo Valentina ◽  
Riganti Chiara ◽  
...  
Keyword(s):  
Cell Culture ◽  
Colon Cancer ◽  
Drug Resistance ◽  
Cancer Cells ◽  
Real Time ◽  
Colon Cancer Cells ◽  
Culture Monitoring

scholarly journals Transistor in a tube: A route to three-dimensional bioelectronics

2018 ◽  
Vol 4 (10) ◽  
pp. eaat4253 ◽  
Author(s):  
C. Pitsalidis ◽  
M. P. Ferro ◽  
D. Iandolo ◽  
L. Tzounis ◽  
S. Inal ◽  
...  
Keyword(s):  
Cell Culture ◽  
Real Time ◽  
Conducting Polymer ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Biological Tissues ◽  
Tissue Formation ◽  
Polymer Scaffold ◽  
Real Time Monitoring ◽  
Electrochemical Transistor

Advances in three-dimensional (3D) cell culture materials and techniques, which more accurately mimic in vivo systems to study biological phenomena, have fostered the development of organ and tissue models. While sophisticated 3D tissues can be generated, technology that can accurately assess the functionality of these complex models in a high-throughput and dynamic manner is not well adapted. Here, we present an organic bioelectronic device based on a conducting polymer scaffold integrated into an electrochemical transistor configuration. This platform supports the dual purpose of enabling 3D cell culture growth and real-time monitoring of the adhesion and growth of cells. We have adapted our system to a 3D tubular geometry facilitating free flow of nutrients, given its relevance in a variety of biological tissues (e.g., vascular, gastrointestinal, and kidney) and processes (e.g., blood flow). This biomimetic transistor in a tube does not require photolithography methods for preparation, allowing facile adaptation to the purpose. We demonstrate that epithelial and fibroblast cells grow readily and form tissue-like architectures within the conducting polymer scaffold that constitutes the channel of the transistor. The process of tissue formation inside the conducting polymer channel gradually modulates the transistor characteristics. Correlating the real-time changes in the steady-state characteristics of the transistor with the growth of the cultured tissue, we extract valuable insights regarding the transients of tissue formation. Our biomimetic platform enabling label-free, dynamic, and in situ measurements illustrates the potential for real-time monitoring of 3D cell culture and compatibility for use in long-term organ-on-chip platforms.

Real-time cell culture monitoring by means of lensfree video microscopy

2014 ◽  
Author(s):  
Srikanth Vinjimore Kesavan ◽  
Cedric Allier ◽  
Fabien Momey ◽  
Fabrice Navarro ◽  
Olivier Cioni ◽  
...  
Keyword(s):  
Cell Culture ◽  
Real Time ◽  
Video Microscopy ◽  
Culture Monitoring

scholarly journals Real-time monitoring of liver fibrosis through embedded sensors in a microphysiological system

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Hafiz Muhammad Umer Farooqi ◽  
Bohye Kang ◽  
Muhammad Asad Ullah Khalid ◽  
Abdul Rahim Chethikkattuveli Salih ◽  
Kinam Hyun ◽  
...  
Keyword(s):  
Cell Culture ◽  
Liver Fibrosis ◽  
Real Time ◽  
Hepatic Fibrosis ◽  
Transforming Growth Factor ◽  
Cell Monolayer ◽  
Embedded Sensors ◽  
Matrix Deposition ◽  
Tgf Β1

AbstractHepatic fibrosis is a foreshadowing of future adverse events like liver cirrhosis, liver failure, and cancer. Hepatic stellate cell activation is the main event of liver fibrosis, which results in excessive extracellular matrix deposition and hepatic parenchyma's disintegration. Several biochemical and molecular assays have been introduced for in vitro study of the hepatic fibrosis progression. However, they do not forecast real-time events happening to the in vitro models. Trans-epithelial electrical resistance (TEER) is used in cell culture science to measure cell monolayer barrier integrity. Herein, we explored TEER measurement's utility for monitoring fibrosis development in a dynamic cell culture microphysiological system. Immortal HepG2 cells and fibroblasts were co-cultured, and transforming growth factor β1 (TGF-β1) was used as a fibrosis stimulus to create a liver fibrosis-on-chip model. A glass chip-based embedded TEER and reactive oxygen species (ROS) sensors were employed to gauge the effect of TGF-β1 within the microphysiological system, which promotes a positive feedback response in fibrosis development. Furthermore, albumin, Urea, CYP450 measurements, and immunofluorescent microscopy were performed to correlate the following data with embedded sensors responses. We found that chip embedded electrochemical sensors could be used as a potential substitute for conventional end-point assays for studying fibrosis in microphysiological systems.

scholarly journals Real-time imaging of cellular forces using optical interference

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Andrew T. Meek ◽  
Nils M. Kronenberg ◽  
Andrew Morton ◽  
Philipp Liehm ◽  
Jan Murawski ◽  
...  
Keyword(s):  
Cell Culture ◽  
Real Time ◽  
High Throughput ◽  
Mechanical Activity ◽  
Dynamic Processes ◽  
Imaging Method ◽  
Neonatal Cardiomyocytes ◽  
A Cell ◽  
Cellular Forces ◽  
Time Acquisition

AbstractImportant dynamic processes in mechanobiology remain elusive due to a lack of tools to image the small cellular forces at play with sufficient speed and throughput. Here, we introduce a fast, interference-based force imaging method that uses the illumination of an elastic deformable microcavity with two rapidly alternating wavelengths to map forces. We show real-time acquisition and processing of data, obtain images of mechanical activity while scanning across a cell culture, and investigate sub-second fluctuations of the piconewton forces exerted by macrophage podosomes. We also demonstrate force imaging of beating neonatal cardiomyocytes at 100 fps which reveals mechanical aspects of spontaneous oscillatory contraction waves in between the main contraction cycles. These examples illustrate the wider potential of our technique for monitoring cellular forces with high throughput and excellent temporal resolution.

scholarly journals Detection of enterovirus in mussels from Morocco by cell culture and real-time PCR

2017 ◽  
Vol 16 (34) ◽  
pp. 1791-1799 ◽  
Author(s):  
Meryem Idrissi Azzouzi Lalla ◽  
Senouci Samira ◽  
El Qazoui Maria ◽  
Oumzil Hicham ◽  
Naciri Mariam
Keyword(s):  
Cell Culture ◽  
Real Time ◽  
Real Time Pcr
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