scholarly journals Chlorine Tolerance and Inactivation of Escherichia coli recovered from Wastewater Treatment Plants in the Eastern Cape, South Africa

2017 ◽  
Vol 7 (8) ◽  
pp. 810 ◽  
Author(s):  
Mojisola Owoseni ◽  
Ademola Olaniran ◽  
Anthony Okoh
Keyword(s):  
Escherichia Coli ◽  
South Africa ◽  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Eastern Cape

scholarly journals Isolation and antibiotic profile ofVibriospp. in final effluents of two wastewater treatment plants in the Eastern Cape of South Africa

2018 ◽  
Author(s):  
Olayinka Osuolale ◽  
Anthony Okoh
Keyword(s):  
South Africa ◽  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Treated Wastewater ◽  
Eastern Cape ◽  
Sources Of Pollution ◽  
Antibiotic Drugs ◽  
Treated Effluents ◽  
Vibrio Spp

ABSTRACTBackgroundPoorly or partially treated wastewater disposed of can contaminate water and even properly treated sewage can have its problems. The highlight of this danger is wastewater treatment plants serving as reservoir for proliferation of antibiotic resistant organisms. We have reported the state of two wastewater treatment in the Eastern Cape of South Africa which discharge poorly and partially treated effluents. Our aims to identify Vibrio spp. and their antibiotic profiles in treated final effluent discharge from wastewater treatment plant.MethodsCulture based approach using the TCBS agar for isolationVibriospp., presumptive isolates were purified and confirmed using PCR. The confirmed isolated were also genotyped to identify the species present. The antibiotic profiling of the confirmed isolates was using the CLSI recommended first line antibiotics for Vibrio.ResultsOut of the 786 presumptive isolates, 374 were confirmed asVibriospp. None of the Vibrio spp. pathotypes were present in the confirmed isolates. Randomized isolates of 100 Vibrio spp. were selected, > 90 % of the isolates were susceptible to Ciprofloxacin, and > 50 – 80 % for Ampicillin, Chloramphenicol, Tetracycline, Cefotaxime, and Trimethoprim-sulfamethoxazole respectively.ConclusionsWe are able to isolate Vibrio spp. from treated effluents but none of their pathotypes were present. The antibiotic agents considered for primary testing which are ciprofloxacin was the most effective of the antibiotic drugs, followed by cefotaxime, tetracycline with less susceptibility. Contamination from discharged effluents from wastewater treatment can lead to spread of spread of disease in this environment. The WWTPs studied are sources of pollution to surface water with environmental and public health.

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