scholarly journals Bioimaging Using Full Field and Contact EUV and SXR Microscopes with Nanometer Spatial Resolution

2017 ◽  
Vol 7 (6) ◽  
pp. 548 ◽  
Author(s):  
Przemysław Wachulak ◽  
Alfio Torrisi ◽  
Mesfin Ayele ◽  
Joanna Czwartos ◽  
Andrzej Bartnik ◽  
...  
Author(s):  
Leslie M. Phinney ◽  
Wei-Yang Lu ◽  
Justin R. Serrano

This paper reports and compares Raman and infrared thermometry measurements along the legs and on the shuttle of a SOI (silicon on insulator) bent-beam thermal microactuator. Raman thermometry offers micron spatial resolution and measurement uncertainties of ±10 K. Typical data collection times are a minute per location leading to measurement times on the order of hours for a complete temperature profile. Infrared thermometry obtains a full-field measurement so the data collection time is on the order of a minute. The spatial resolution is determined by the pixel size, 25 μm by 25 μm for the system used, and infrared thermometry also has uncertainties of ±10 K after calibration with a nonpackaged sample. The Raman and infrared measured temperatures agreed both qualitatively and quantitatively. For example, when the thermal microactuator was operated at 7 V, the peak temperature on an interior leg is 437 K ± 10 K and 433 K ± 10 K from Raman and infrared thermometry, respectively. The two techniques are complementary for microsystems characterization when infrared imaging obtains a full-field temperature measurement and Raman thermometry interrogates regions for which higher spatial resolution is required.


2007 ◽  
Author(s):  
Courtney A. Brewer ◽  
Fernando Brizuela ◽  
Dale Martz ◽  
Georgiy Vaschenko ◽  
Mario C. Marconi ◽  
...  

Author(s):  
C. W. Han ◽  
S. Cho ◽  
B. Han

Moire´ interferometry is a full-field optical method that has high displacement, strain and spatial resolution. The method has been used extensively for deformation analyses in the various fields of mechanics. Special considerations arise when deformation measurements of tiny specimens or tiny regions of larger specimens are sought. The relative displacements within a small field of view will be small (even if the strains are not small), so the number of morie´ fringes might not be enough for an accurate analysis.


2019 ◽  
Vol 205 ◽  
pp. 05015
Author(s):  
Mancini Giulia F. ◽  
Robert Karl ◽  
Shanblatt Elisabeth ◽  
Bevis Charles ◽  
Gardner Dennis ◽  
...  

We demonstrate full-field quantitative ptychographic imaging using tabletop high harmonics to visualize the extended structure of silica close-packed nanosphere multilayers with <20nm spatial resolution, and also extract small-angle EUV Bragg scattering order/disorder correlations.


2019 ◽  
Vol 26 (2) ◽  
pp. 571-584 ◽  
Author(s):  
Steven J. Leake ◽  
Gilbert A. Chahine ◽  
Hamid Djazouli ◽  
Tao Zhou ◽  
Carsten Richter ◽  
...  

The ID01 beamline has been built to combine Bragg diffraction with imaging techniques to produce a strain and mosaicity microscope for materials in their native or operando state. A scanning probe with nano-focused beams, objective-lens-based full-field microscopy and coherent diffraction imaging provide a suite of tools which deliver micrometre to few nanometre spatial resolution combined with 10−5 strain and 10−3 tilt sensitivity. A detailed description of the beamline from source to sample is provided and serves as a reference for the user community. The anticipated impact of the impending upgrade to the ESRF – Extremely Brilliant Source is also discussed.


2018 ◽  
Vol 63 (7) ◽  
pp. 075006 ◽  
Author(s):  
Shouhua Luo ◽  
Tao Shen ◽  
Yi Sun ◽  
Jing Li ◽  
Guang Li ◽  
...  

2019 ◽  
Vol 5 (11) ◽  
pp. 83
Author(s):  
Qimei Zhang ◽  
Anna M. Grabowska ◽  
Philip A. Clarke ◽  
Stephen P. Morgan

The spatial resolution and light detected in fluorescence imaging for small animals are limited by light scattering, absorption and autofluorescence. To address this, novel near-infrared fluorescent contrast agents and imaging configurations have been investigated. In this paper, the influence of the light wavelength and imaging configurations (full-field illumination system and scanning system) on fluorescence imaging are compared quantitatively. The surface radiance for both systems is calculated by modifying the simulation tool Near-Infrared Fluorescence and Spectral Tomography. Fluorescent targets are embedded within a scattering medium at different positions. The surface radiance and spatial resolution are obtained for emission wavelengths between 620 nm and 1000 nm. It was found that the spatial resolution of the scanning system is independent of the tissue optical properties, whereas for full-field illumination, the spatial resolution degrades at longer wavelength. The full width at half maximum obtained by the scanning system is 25% lower than that obtained by the full-field illumination system when the targets are located in the middle of the phantom. The results indicate that although imaging at near-infrared wavelength can achieve a higher surface radiance, it may produce worse spatial resolution.


2016 ◽  
Vol 41 (17) ◽  
pp. 3920 ◽  
Author(s):  
Peng Xiao ◽  
Mathias Fink ◽  
A. Claude Boccara

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