scholarly journals Enhanced Agarose and Xylan Degradation for Production of Polyhydroxyalkanoates by Co-Culture of Marine Bacterium, Saccharophagus degradans and Its Contaminant, Bacillus cereus

2017 ◽  
Vol 7 (3) ◽  
pp. 225 ◽  
Author(s):  
Shailesh Sawant ◽  
Bipinchandra Salunke ◽  
Larry Taylor ◽  
Beom Kim
Keyword(s):  
Bacillus Cereus ◽  
Marine Bacterium ◽  
Saccharophagus Degradans ◽  
Xylan Degradation

scholarly journals A Novel Glycoside Hydrolase Family 5 β-1,3-1,6-Endoglucanase from Saccharophagus degradans 2-40Tand Its Transglycosylase Activity

2016 ◽  
Vol 82 (14) ◽  
pp. 4340-4349 ◽  
Author(s):  
Damao Wang ◽  
Do Hyoung Kim ◽  
Nari Seo ◽  
Eun Ju Yun ◽  
Hyun Joo An ◽  
...  
Keyword(s):  
Glycoside Hydrolase ◽  
Marine Bacterium ◽  
Enzymatic Reaction ◽  
Glycoside Hydrolase Family ◽  
Reaction Products ◽  
Brown Macroalgae ◽  
Fungal Cell ◽  
Content Type ◽  
Saccharophagus Degradans ◽  
Hydrolase Family

ABSTRACTIn this study, we characterized Gly5M, originating from a marine bacterium, as a novel β-1,3-1,6-endoglucanase in glycoside hydrolase family 5 (GH5) in the Carbohydrate-Active enZyme database. Thegly5Mgene encodes Gly5M, a newly characterized enzyme from GH5 subfamily 47 (GH5_47) inSaccharophagus degradans2-40T. Thegly5Mgene was cloned and overexpressed inEscherichia coli. Through analysis of the enzymatic reaction products by thin-layer chromatography, high-performance liquid chromatography, and matrix-assisted laser desorption ionization–tandem time of flight mass spectrometry, Gly5M was identified as a novel β-1,3-endoglucanase (EC 3.2.1.39) and bacterial β-1,6-glucanase (EC 3.2.1.75) in GH5. The β-1,3-endoglucanase and β-1,6-endoglucanase activities were detected by using laminarin (a β-1,3-glucan with β-1,6-glycosidic linkages derived from brown macroalgae) and pustulan (a β-1,6-glucan derived from fungal cell walls) as the substrates, respectively. This enzyme also showed transglycosylase activity toward β-1,3-oligosaccharides when laminarioligosaccharides were used as the substrates. Since laminarin is the major form of glucan storage in brown macroalgae, Gly5M could be used to produce glucose and laminarioligosaccharides, using brown macroalgae, for industrial purposes.IMPORTANCEIn this study, we have discovered a novel β-1,3-1,6-endoglucanase with a unique transglycosylase activity, namely, Gly5M, from a marine bacterium,Saccharophagus degradans2-40T. Gly5M was identified as the newly found β-1,3-endoglucanase and bacterial β-1,6-glucanase in GH5. Gly5M is capable of cleaving glycosidic linkages of both β-1,3-glucans and β-1,6-glucans. Gly5M also possesses a transglycosylase activity toward β-1,3-oligosacchrides. Due to the broad specificity of Gly5M, this enzyme can be used to produce glucose or high-value β-1,3- and/or β-1,6-oligosaccharides.

scholarly journals Biosynthesis of Extracellular Polymeric Substances by the Marine BacteriumSaccharophagus degradansunder Different Nutritional Conditions

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Yolanda González-García ◽  
Alejandra Heredia ◽  
Juan Carlos Meza-Contreras ◽  
Froylán M. E. Escalante ◽  
Rosa María Camacho-Ruiz ◽  
...  
Keyword(s):  
Carbon Source ◽  
Synergistic Effect ◽  
Maximum Concentration ◽  
Marine Bacterium ◽  
Extracellular Polymeric Substances ◽  
Mineral Medium ◽  
Saccharophagus Degradans ◽  
First Report ◽  
Nutritional Conditions ◽  
New Feature

The effect of carbon source, carbon to nitrogen (C/N) ratio, and limitation in nutrients (N, P, K, Ca, Mg, and Fe) on extracellular polymeric substances (EPS) synthesis by the marine bacteriumSaccharophagus degradanswas studied. This strain was able to grow in mineral medium and produce EPS with different efficiency according to the C source used (g EPS/L): glucose or starch (1.5 ± 0.2); galactose, sucrose, or xylose (0.7 ± 0.2); and fructose (0.3 ± 0.1). The C/N ratio (glucose/ammonium) had a significant effect on EPS biosynthesis due to its production rise as the C/N ratio increased from 3 to 100 (0.7 to 2.1 g EPS/L). It was also observed that limitation in nutrients such as N, P, K, Ca, Mg, and Fe also favored EPS biosynthesis. When taking into account both factors (C/N ratio, 100; nutrients limitation, 50%) a positive synergistic effect was noted on EPS production since under these conditions the maximum concentration obtained was 4.12 ± 0.3 g/L after 72 h of culture. The polymer was found to be a polysaccharide of mainly glucose, mannose, and galactose. This is the first report on EPS production byS. degradanswhich is a new feature of this versatile marine bacterium.

Biosynthesis and characterization of polyhydroxyalkanoates in the polysaccharide-degrading marine bacterium Saccharophagus degradans ATCC 43961

2008 ◽  
Vol 35 (6) ◽  
pp. 629-633 ◽  
Author(s):  
Yolanda González-García ◽  
Jesús Nungaray ◽  
Jesús Córdova ◽  
Orfil González-Reynoso ◽  
Martin Koller ◽  
...  
Keyword(s):  
Marine Bacterium ◽  
Saccharophagus Degradans

scholarly journals Complete Genome Sequence of the Complex Carbohydrate-Degrading Marine Bacterium, Saccharophagus degradans Strain 2-40T

PLoS Genetics ◽  
2008 ◽  
Vol 4 (5) ◽  
pp. e1000087 ◽  
Author(s):  
Ronald M. Weiner ◽  
Larry E. Taylor ◽  
Bernard Henrissat ◽  
Loren Hauser ◽  
Miriam Land ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Marine Bacterium ◽  
Complex Carbohydrate ◽  
Saccharophagus Degradans

scholarly journals Processive Endoglucanases Mediate Degradation of Cellulose by Saccharophagus degradans

2009 ◽  
Vol 191 (18) ◽  
pp. 5697-5705 ◽  
Author(s):  
Brian J. Watson ◽  
Haitao Zhang ◽  
Atkinson G. Longmire ◽  
Young Hwan Moon ◽  
Steven W. Hutcheson
Keyword(s):  
Marine Bacterium ◽  
Saccharophagus Degradans ◽  
Cellulosic Materials ◽  
Independent Mechanism ◽  
Bacteria And Fungi ◽  
The One ◽  
Secreted Enzymes

ABSTRACT Bacteria and fungi are thought to degrade cellulose through the activity of either a complexed or a noncomplexed cellulolytic system composed of endoglucanases and cellobiohydrolases. The marine bacterium Saccharophagus degradans 2-40 produces a multicomponent cellulolytic system that is unusual in its abundance of GH5-containing endoglucanases. Secreted enzymes of this bacterium release high levels of cellobiose from cellulosic materials. Through cloning and purification, the predicted biochemical activities of the one annotated cellobiohydrolase Cel6A and the GH5-containing endoglucanases were evaluated. Cel6A was shown to be a classic endoglucanase, but Cel5H showed significantly higher activity on several types of cellulose, was the highest expressed, and processively released cellobiose from cellulosic substrates. Cel5G, Cel5H, and Cel5J were found to be members of a separate phylogenetic clade and were all shown to be processive. The processive endoglucanases are functionally equivalent to the endoglucanases and cellobiohydrolases required for other cellulolytic systems, thus providing a cellobiohydrolase-independent mechanism for this bacterium to convert cellulose to glucose.

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