scholarly journals Antibacterial Fluorinated Diamond-Like Carbon Coating Promotes Osteogenesis—Comparison with Titanium Alloy

2021 ◽  
Vol 11 (20) ◽  
pp. 9451
Author(s):  
Takeshi Sasamoto ◽  
Masahito Kawaguchi ◽  
Katsutaka Yonezawa ◽  
Toru Ichiseki ◽  
Ayumi Kaneuji ◽  
...  
Keyword(s):  
Titanium Alloy ◽  
Bone Formation ◽  
Antibacterial Properties ◽  
Diamond Like Carbon ◽  
Bone Surface ◽  
Beagle Dogs ◽  
Trabecular Thickness ◽  
Dlc Coating ◽  
In Vivo Bone Formation

Fluorinated diamond-like carbon (F-DLC) coating is biologically safe, provides superior antibacterial properties, and shows promise in preventing postoperative peri-implant infections. However, potential negative effects of this coating on in vivo bone formation and resorption have not been studied. The authors investigated the effects of F-DLC coatings on bone union in beagle dogs. Seventy-two solid columns of titanium alloy were prepared with equally spaced slits. Half of these columns were coated with F-DLC (Group F), and the others were left uncoated as controls (Group C). Columns were implanted in the femurs of beagle dogs, and in vivo bone formation and resorption were assessed 4, 8, and 12 weeks after implantation. In comparison to Group C, Group F showed significantly greater bone volume and trabecular thickness at Week 8 (p < 0.05) and Week 12 (p < 0.005) and significantly lower bone resorption activity, measured by the ratio of osteoclasts to bone surface and of eroded surface to bone surface, at Week 12 (p < 0.05). The F-DLC coating encouraged bone formation in vivo more effectively than uncoated titanium alloy, suggesting that F-DLC will prove to be a useful coating material for antibacterial intraosseous implants.

In Vivo Bone Formation Following Transplantation of Human Adipose–Derived Stromal Cells That Are Not Differentiated Osteogenically

2008 ◽  
Vol 14 (8) ◽  
pp. 1285-1294 ◽  
Author(s):  
Oju Jeon ◽  
Jong Won Rhie ◽  
Il-Kuen Kwon ◽  
Jae-Hwan Kim ◽  
Byung-Soo Kim ◽  
...  
Keyword(s):  
Bone Formation ◽  
Stromal Cells ◽  
Adipose Derived Stromal Cells ◽  
In Vivo Bone Formation

scholarly journals A Delphinidin-Enriched Maqui Berry Extract Improves Bone Metabolism and Protects against Bone Loss in Osteopenic Mouse Models

Antioxidants ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 386 ◽  
Author(s):  
Masahiro Nagaoka ◽  
Toyonobu Maeda ◽  
Masahiro Chatani ◽  
Kazuaki Handa ◽  
Tomoyuki Yamakawa ◽  
...  
Keyword(s):  
Bone Loss ◽  
Bone Formation ◽  
Bone Metabolism ◽  
Mouse Models ◽  
Bone Morphogenetic Protein 2 ◽  
Bone Surface ◽  
Bone Formation Rate ◽  
Trabecular Thickness ◽  
Tissue Volume ◽  
Maqui Berry

In our previous investigation, delphinidin, one of the most abundant anthocyanins found in vegetables and berry fruits, had been shown to inhibit osteoclasts and prevent bone loss in mouse models of osteoporosis. In the present study, we investigated whether a delphinidin glycoside-enriched maqui berry extract (MBE, Delphinol®) exhibits beneficial effects on bone metabolism both in vitro and in vivo. MBE stimulated the osteoblastic differentiation of MC3T3-E1 cells, as indicated by enhanced mineralized nodule formation, and increased alkaline phosphatase activity, through the upregulation of bone morphogenetic protein 2 (Bmp2), runt-related transcription factor 2 (Runx2), Osterix (Osx), osteocalcin (Ocn), and matrix extracellular phosphoglycoprotein (Mepe) mRNA expression. Immunostaining and immunoprecipitation assays demonstrated that MBE suppressed NF-κB transnucleation through acting as a superoxide anion/peroxynitrite scavenger in MC3T3-E1 cells. Simultaneously, MBE inhibited both osteoclastogenesis in primary bone marrow macrophages and pit formation by maturated osteoclasts on dentine slices. Microcomputed tomography (micro-CT) and bone histomorphometry analyses of femurs demonstrated that the daily ingestion of MBE significantly increased BV/TV (ratio of bone volume to tissue volume), Tb.Th (trabecular thickness), Tb.N (trabecular number), N.Nd/N.Tm (node to terminus ratio), OV/TV (ratio of osteoid volume to tissue volume), BFR/TV (bone formation rate per tissue volume), and significantly decreased Tb.Sp (trabecular separation), ES/BS (ratio of eroded surface to bone surface) and N.Oc/BS (number of osteoclast per unit of bone surface), compared to vehicle controls in osteopenic mouse models. These findings suggest that MBE can be a promising natural agent for the prevention of bone loss in osteopenic conditions by not only inhibiting bone resorption, but also stimulating bone formation.

scholarly journals Synthetic design of growth factor sequestering extracellular matrix mimetic hydrogel for promoting in vivo bone formation

Biomaterials ◽  
2018 ◽  
Vol 161 ◽  
pp. 190-202 ◽  
Author(s):  
Hong Ji Yan ◽  
Tommaso Casalini ◽  
Gry Hulsart-Billström ◽  
Shujiang Wang ◽  
Oommen P. Oommen ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Growth Factor ◽  
Bone Formation ◽  
In Vivo Bone Formation

scholarly journals Early Biofilm Formation on UV Light Activated Nanoporous TiO2SurfacesIn Vivo

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Nagat Areid ◽  
Eva Söderling ◽  
Johanna Tanner ◽  
Ilkka Kangasniemi ◽  
Timo O. Närhi
Keyword(s):  
Titanium Alloy ◽  
Biofilm Formation ◽  
Uv Light ◽  
Antibacterial Properties ◽  
Mutans Streptococci ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Biofilm Development

Purpose. To explore earlyS. mutansbiofilm formation on hydrothermally induced nanoporous TiO2surfacesin vivoand to examine the effect of UV light activation on the biofilm development.Materials and Methods. Ti-6Al-4V titanium alloy discs (n = 40) were divided into four groups with different surface treatments: noncoated titanium alloy (NC); UV treated noncoated titanium alloy (UVNC); hydrothermally induced TiO2coating (HT); and UV treated titanium alloy with hydrothermally induced TiO2coating (UVHT).In vivoplaque formation was studied in 10 healthy, nonsmoking adult volunteers. Titanium discs were randomly distributed among the maxillary first and second molars. UV treatment was administered for 60 min immediately before attaching the discs in subjects’ molars. Plaque samples were collected 24h after the attachment of the specimens. Mutans streptococci (MS), non-mutans streptococci, and total facultative bacteria were cultured, and colonies were counted.Results. The plaque samples of NC (NC + UVNC) surfaces showed over 2 times more oftenS. mutanswhen compared to TiO2surfaces (HT + UVHT), with the number of colonized surfaces equal to 7 and 3, respectively.Conclusion. Thisin vivostudy suggested that HT TiO2surfaces, which we earlier showed to improve blood coagulation and encourage human gingival fibroblast attachmentin vitro, do not enhance salivary microbial (mostly mutans streptococci) adhesion and initial biofilm formation when compared with noncoated titanium alloy. UV light treatment provided Ti-6Al-4V surfaces with antibacterial properties and showed a trend towards less biofilm formation when compared with non-UV treated titanium surfaces.

ONO-1301 Enhances in vitro Osteoblast Differentiation and in vivo Bone Formation Induced by Bone Morphogenetic Protein

Spine ◽  
2018 ◽  
Vol 43 (11) ◽  
pp. E616-E624 ◽  
Author(s):  
Sadaaki Kanayama ◽  
Takashi Kaito ◽  
Kazuma Kitaguchi ◽  
Hiroyuki Ishiguro ◽  
Kunihiko Hashimoto ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Morphogenetic Protein ◽  
Osteoblast Differentiation ◽  
Morphogenetic Protein ◽  
In Vivo Bone Formation

scholarly journals Association between in vivo bone formation and ex vivo migratory capacity of human bone marrow stromal cells

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Rikke K. Andersen ◽  
Walid Zaher ◽  
Kenneth H. Larsen ◽  
Nicholas Ditzel ◽  
Katharina Drews ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Formation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Ex Vivo ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Migratory Capacity ◽  
In Vivo Bone Formation
Sign in / Sign up

Export Citation Format

Share Document