scholarly journals Phytochemical Constituents and Biological Activities of the Unexplored Plant Rhinanthus angustifolius subsp. grandiflorus

2021 ◽  
Vol 11 (19) ◽  
pp. 9162
Author(s):  
Leilei Zhang ◽  
Gokhan Zengin ◽  
Gabriele Rocchetti ◽  
Ismail Senkardes ◽  
Jugreet B. Sharmeen ◽  
...  
Keyword(s):  
High Resolution Mass Spectrometry ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Water Extract ◽  
The Other ◽  
Total Phenolic ◽  
Water Extracts ◽  
Extraction Solvent ◽  
Rhinanthus Angustifolius ◽  
Untargeted Profiling

In the present study, a total of 12 extracts of Rhinanthus angustifolius subsp. grandiflorus, an understudied hemiparasitic species, were obtained using different extraction techniques, namely, homogenizer-assisted extraction (HAE), maceration (MAC), soxhlet (SOX), infusion, and solvents (ethyl acetate, methanol, ethanol, and water), and were evaluated for their in vitro antioxidant and enzyme-inhibiting properties. Additionally, untargeted profiling based on high-resolution mass spectrometry targeted different phytochemical classes, namely, polyphenols, terpenoids, and alkaloids. The highest total phenolic and flavonoid contents were detected using methanol as the extraction solvent. Multivariate statistics following the untargeted profiling revealed that the extraction solvent had a hierarchically higher impact than the extraction method when considering the recovery of bioactive compounds. The methanolic extracts displayed the highest radical-scavenging antioxidant capacity, as provided by CUPRAC and FRAP assays. On the other hand, the water extracts (MAC and HAE) and the infusion extract showed the highest activity as metal chelators (25.66–27.51 mg EDTAE/g). Similarly, the water extract obtained by HAE and the infusion extract revealed the highest phosphomolybdenum activity (3.92 ± 0.14 and 3.71 ± 0.01 mmol TE/g, respectively). The different extracts also exhibited different enzyme inhibition potentials. For instance, HAE and MAC ethanolic extracts inhibited only α-amylase (0.69 ± 0.01 and 0.70 ± 0.01 mmol ACAE/g), while all the other extracts showed a dual inhibition against both carbohydrate-hydrolyzing enzymes tested (i.e., α-amylase: 0.07–0.69 mmol ACAE/g; α-glucosidase: 0.03–1.30 mmol ACAE/g). Nevertheless, the other extracts inhibited acetyl-, butyryl-cholinesterases, or both; MAC–water extract displayed no inhibition against the enzymes. Additionally, all the studied extracts were found to inhibit tyrosinase, ranging from 10.62 to 52.80 mg KAE/g. In general, the water extracts showed weaker inhibition towards the enzymes than the other extracts. This study demonstrated that R. angustifolius is an excellent source of natural antioxidants and enzyme inhibitors that could be further investigated and exploited for pharmaceutical purposes.

scholarly journals Comprehensive Biological and Chemical Evaluation of Two Seseli Species (S. gummiferum and S. transcaucasicum)

Antioxidants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1510
Author(s):  
Gokhan Zengin ◽  
Dejan Stojković ◽  
Mohamad Fawzi Mahomoodally ◽  
Bibi Sharmeen Jugreet ◽  
Mehmet Yavuz Paksoy ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Water Extract ◽  
Herbal Remedies ◽  
Total Phenolic ◽  
Hacat Cells ◽  
Medicinal Uses ◽  
Water Extracts ◽  
Methanolic Extracts

Seseli L. is one of the largest genera of the Apiaceae family widely known for their traditional uses as herbal remedies. In the present study, the methanolic and water extracts of two Seseli species, S. gummiferum and S. transcaucasicum were evaluated for their bioactive contents and biological activities. The total phenolic and flavonoid contents in the extracts ranged from 19.09 to 24.33 mg GAE/g and from 0.45 to10.09 mg RE/g, respectively. Moreover, while narcissin was detected as the most abundant component in the methanolic extract of S. transcaucasicum (261.40 µg/g), chlorogenic acid was identified as the major component in all the other extracts, although a high amount was also present in the methanolic S. transcaucasicum extract (107.48–243.12 µg/g). The total antioxidant capacity was also determined by the phosphomolybdenum assay (0.66–1.18 mM TE/g). Other antioxidant assays such as the radical scavenging assays (DPPH: 5.51–11.45 mg TE/g; ABTS: 43.46–51.91 mg TE/g), reducing assays (CUPRAC: 41.67–53.20 mg TE/g; FRAP: 31.26–34.14 mg TE/g), as well as the metal chelating activity assay (14.38–38.57 mg EDTAE/g) were conducted. All the extracts showed inhibitory potential against the enzyme’s amylase (0.12–0.78 mM ACAE/g), acetyl- and butyryl-cholinesterase (0.15–9.71 mg GALAE/g), while only the methanolic extracts acted as inhibitors of tyrosinase (107.15 and 109.37 mg KAE/g) and only the water extract of S. gummiferum displayed anti-glucosidase activity (0.13 mM ACAE/g). Interestingly, the methanolic extracts of both Seseli species showed lower cytotoxicity towards HaCaT cells (IC50: >500 µg/mL), compared to the water extracts (IC50: 267.8 and 321.41 µg/mL). Besides, only the methanolic extracts showed a slight wound healing effect (28.21 and 31.23%). All extracts showed antibacterial action against Staphylococcus lugdunensis (minimum inhibitory and bactericidal concentrations: 0.025–2 mg/mL). S. gummiferum methanolic extract, which exhibited the highest antibacterial potency was found to inhibit adhesion and invasion of S. lugdunensis to HaCaT cells as well. Taken together, this study demonstrated the two Seseli species to harbour interesting bioactive components, in particular polyphenolics and to exhibit several biological properties that could be further investigated for their potential exploitation as healing agents as supported by various traditional medicinal uses.

scholarly journals Phytochemical, Antibacterial, and Antioxidant Activities of Anthurium Hookerii leaves Extracts

2019 ◽  
Vol 26 (3) ◽  
pp. 101
Author(s):  
Atmira Sariwati ◽  
Inayah Fitri ◽  
Adi Setyo Purnomo ◽  
Sri Fatmawati
Keyword(s):  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Water Extract ◽  
Maximum Diameter ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Total Phenol Content ◽  
Water Extracts ◽  
The Family ◽  
Antibacterial Potential

Many plants of the family of Araceae possess significant benefit as medicinal plants. Anthurium hookerii is herbaceous genus of the family of Araceae. A. hookerii leaves were extracted with five dissimilarity solvents (methanolic, water, ethyl acetate, n-hexane, and dichloromethane). The extracts were evaluated for their phytochemical, total phenolic contents, and antibacterial potential. The presences of tannins and saponins were found in all crude extracts. The steroid was only found in dichloromethane extract, whereas flavonoid was obtained in methanol and water extracts. Besides; methanol, ethyl acetate, water, and n-hexane extracts showed triterpenoid contents. Alkaloid presences in ethyl acetate, methanolic, dichloromethane, and water extracts. The total phenol content was examined by Follin-Ciocalteu assay, which varied from 9.52-76.56 mg/g GAE. The highest total phenolic was found in methanol extract. Antioxidant activity was calculated based on diphenyl picryl hydrazyl radical scavenging ability that showed the scavenging activity with range 7.24-66.11%, which the methanoilic extract have the excellent antioxidant potential (IC50 232.90 µg/ml). Antibacterial activity of leaves extracts of A. hookerii was screened based on disc diffusion method. Water extract showed the wide spectrum antibacterial potential. Klebsiella sp., Bacillus subtilis, Pripioni agnes, and Strepticoccus mutans with maximum diameter of inhibition zone 10.30, 14.20, 9.60, and 15.10 mm, respectively.

scholarly journals Propiedades antioxidante y antiglicosilante de extractos de Diplotaxis tenuifolia

Bionatura ◽  
2019 ◽  
Vol 4 (2) ◽  
pp. 852-855
Author(s):  
Romina B Parada ◽  
Marisol Vallejo ◽  
Emilio R Marguet
Keyword(s):  
Ascorbic Acid ◽  
Maillard Reaction ◽  
Dry Matter ◽  
Biological Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Water Extract ◽  
Edible Plant ◽  
Water Extracts ◽  
Trolox Equivalent

The goal of this study was to investigate phenolic contents and biological activities in leaves of Diplotaxis tenuifolia collected in Patagonia (Argentina). Anti-glycation activities were tested by bovine serum albumin-glucose (BSA-glu) and Maillard reaction models. Only in the case of water extract of dry matter, Maillard reaction could be 40 % inhibited. The remaining results do not allow make definitive conclusions. Water extracts exhibited higher polyphenol content than methanolic ones (1074.25 and 647.5 mg Gallic acid equivalent/100 g of dry matter, respectively). On the other hand, flavonoid content was comparable between the water and methanolic extracts (218.2 and 213.4 mg quercetin equivalent/100 g of dry matter, respectively). Regarding antioxidant properties, CUPRAC (Cupric reducing antioxidant capacity) showed values of 655.6 mg Trolox equivalent/100 g of dry matter and 749.6 mg ascorbic acid equivalent/100 g of dry matter for water extracts and 1045,2 mg Trolox equivalent/100 g of dry matter and 1203,9 mg ascorbic acid equivalent/100 g of dry matter for methanolic ones. DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay displayed values of 203,3 mg Trolox equivalent/100 g of dry matter and 180.0 mg ascorbic acid equivalent/100 g of dry matter for water extracts, and 97.7 mg Trolox equivalent/100 g of dry matter and 89,3 mg ascorbic acid equivalent/100 g of dry matter for methanolic ones. The results obtained highlight the antioxidative potential of D. tenuifolia collected in extreme regions like Patagonia. This edible plant is an antioxidant-rich source that should be considered as functional food, its consumption is recommendable to prevent cell damage by scavenging deleterious free radicals.

scholarly journals Antioxidant capacity and antioxidants of strawberry, blackberry, and raspberry leaves

2011 ◽  
Vol 29 (No. 2) ◽  
pp. 181-189 ◽  
Author(s):  
L. Buřičová ◽  
M. Andjelkovic ◽  
A. Čermáková ◽  
Z. Réblová ◽  
O. Jurček ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Ellagic Acid ◽  
Radical Scavenging ◽  
Water Extract ◽  
Rubus Idaeus ◽  
Total Phenolic ◽  
Water Extracts ◽  
Antioxidant Capacities ◽  
Dpph Method ◽  
Radical Scavenging Ability

The total phenolic content (Folin-Ciocalteu method), free radical scavenging ability expressed as DPPH value, ferric reducing antioxidant capacity (FRAP), and oxygen radical absorbance capacity (ORAC) were determined in water extracts of leaves from Rosaceae family plants (Fragaria vesca L., Rubus fructicosus L., and Rubus idaeus L.). The antioxidant capacities of the extracts (in the order of the above mentioned methods) were 73.6–88.9%, 60.1–71.4%, 49.7–78.0% respectively, and 45.3–66.5% of that of green tea water extract. Further, the presence of 15 compounds (gallic acid, rutin, ellagic acid, caffeic acid, p-coumaric acid, quercetin, kaempferol, myricetin, quercetin-3-d-glucoside, ascorbic acid, (+)-catechin, (–)-epicatechin, epicatechingallate, epigallocatechin, procyanidin B1) was studied by HPLC-ECD and their antioxidant capacities were compared to the antioxidant capacity of the extracts. Out of the compounds studied, mostly (+)-catechin, ellagic acid, and (–)-epicatechin participated in the antioxidant capacities of the studied plant leaves water extracts. The antioxidant capacity of leaves infusions (determined by DPPH method) was lower than those of red wines and tea infusions, but comparable to the antioxidant capacities of white wines and fruit beverages.

scholarly journals LC-HRMS Profiling and Antidiabetic, Anticholinergic, and Antioxidant Activities of Aerial Parts of Kınkor (Ferulago stellata)

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2469
Author(s):  
Hatice Kızıltaş ◽  
Zeynebe Bingol ◽  
Ahmet Ceyhan Gören ◽  
Leyla Polat Kose ◽  
Lokman Durmaz ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
High Resolution Mass Spectrometry ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Water Extract ◽  
Ethanolic Extract ◽  
Metabolic Enzyme ◽  
Scavenging Activity ◽  
Ferrous Ions ◽  
Water Extracts

Kınkor (Ferulago stellata) is Turkish medicinal plant species and used in folk medicine against some diseases. As far as we know, the data are not available on the biological activities and chemical composition of this medicinal plant. In this study, the phytochemical composition; some metabolic enzyme inhibition; and antidiabetic, anticholinergic, and antioxidant activities of this plant were assessed. In order to evaluate the antioxidant activity of evaporated ethanolic extract (EEFS) and lyophilized water extract (WEFS) of kınkor (Ferulago stellata), some putative antioxidant methods such as DPPH· scavenging activity, ABTS•+ scavenging activity, ferric ions (Fe3+) reduction method, cupric ions (Cu2+) reducing capacity, and ferrous ions (Fe2+)-binding activities were separately performed. Furthermore, ascorbic acid, BHT, and α-tocopherol were used as the standard compounds. Additionally, the main phenolic compounds that are responsible for antioxidant abilities of ethanol and water extracts of kınkor (Ferulago stellata) were determined by liquid chromatography-high-resolution mass spectrometry (LC-HRMS). Ethanol and water extracts of kınkor (Ferulago stellata) demonstrated effective antioxidant abilities when compared to standards. Moreover, ethanol extract of kınkor (Ferulago stellata) demonstrated IC50 values of 1.772 μg/mL against acetylcholinesterase (AChE), 33.56 ± 2.96 μg/mL against α-glycosidase, and 0.639 μg/mL against α-amylase enzyme respectively.

Investigation of phytochemicals and antioxidant capacity of selected Eucalyptus species using conventional extraction

2015 ◽  
Vol 0 (0) ◽  
Author(s):  
Deep Jyoti Bhuyan ◽  
Quan V. Vuong ◽  
Anita C. Chalmers ◽  
Ian A. van Altena ◽  
Michael C. Bowyer ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Content ◽  
Bioactive Compound ◽  
The Other ◽  
Total Phenolic ◽  
Eucalyptus Species ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Extraction Solvent ◽  
Conventional Extraction

AbstractEucalyptus species have found their place in traditional medicine and pharmacological research and they have also been shown to possess a large number of phenolic compounds and antioxidants. The present study sought to implement conventional extraction to yield maximal total phenolic content (TPC), total flavonoid content (TFC), proanthocyanidins, antioxidants, and saponins from E. robusta using different solvents. The most suitable extraction solvent was further employed for extracting phytochemicals from E. saligna, E. microcorys, and E. globulus to select the Eucalyptus species with the greatest bioactive compound content. The results emphasised the efficiency of water in extracting TPC ((150.60 ± 2.47) mg of gallic acid equivalents per g), TFC ((38.83 ± 0.23) mg of rutin equivalents per g), proanthocyanidins ((5.14 ± 0.77) mg of catechin equivalents per g), and antioxidants ABTS ((525.67 ± 1.99) mg of trolox equivalents (TE) per g), DPPH ((378.61 ± 4.72) mg of TE per g); CUPRAC ((607.43 ± 6.69) mg of TE per g) from E. robusta. Moreover, the aqueous extract of E. robusta had the highest TPC, TFC and antioxidant values among the other Eucalyptus species tested. These findings highlighted the efficiency of conventional extraction in extracting natural bioactive compounds from Eucalyptus species for pharmaceutical and nutraceutical applications.

The Effects of Solvents and Extraction Methods on the Antioxidant Activity of P. niruri

2014 ◽  
Vol 68 (5) ◽  
Author(s):  
Hafizah Mohd Hadzri ◽  
Mohd Azizi Che Yunus ◽  
Salman Zhari ◽  
Fahim Rithwan
Keyword(s):  
Antioxidant Activity ◽  
Extraction Method ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Soxhlet Extraction ◽  
Solvent Polarity ◽  
Extraction Methods ◽  
The Other ◽  
Natural Antioxidant ◽  
Total Phenolic

The effects of different types of solvents and extraction method were investigated to determine the presence of antioxidant contents and activity from the P. niruri plant. The aim of this study is to determine which extraction method will give higher natural antioxidant contents and antioxidant activity. The content of natural antioxidant and antioxidant activity were analysed by total phenolic content (TPC), total flavonoid content (TFC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity assay. The results showed that extracts from a supercritical fluid extraction (SFE) method without the addition of modifier showed the highest content of total phenolic (187.66 mg GAE/ g) and flavonoid (1100.93 mg QE/g) in P. niruri compared to the other methods of extraction with different type of solvents. The extract of P. niruri from different extraction methods showed antioxidant activity on DPPH radical scavenging assay. The soxhlet extraction method by methanol showed the lowest IC50 compared to the other methods of extraction. The results revealed that P. niruri extracts had different content of antioxidant and antioxidant activity. The solvent polarity and different methods of extraction play significant roles in determining the most suitable method for production of antioxidant contents and antioxidant activity from P. niruri extracts.

scholarly journals Antioxidant Properties and Anti-inflammatory Effects of the Hydroethanolic Extracts of Two Varieties of Bayberry fruit (Myrica rubra Sieb et Zucc.) Prepared by Stirring and Ultrasonic Methods

2019 ◽  
Vol 47 (3) ◽  
Author(s):  
Kuan-Hung LIN ◽  
Chun-Ping LU ◽  
Jia-Wei CHAO ◽  
Yi-Ping YU
Keyword(s):  
Extraction Method ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ultrasonic Extraction ◽  
The Other ◽  
Total Phenolic ◽  
Myrica Rubra ◽  
Ultrasonic Methods ◽  
Anti Inflammatory

Chinese bayberry (Myrica rubra Sieb. et Zucc.) is an economically important medicinal plant with multiple uses. Two varieties ‘Dongkui Oriental Pearl’ (Dongkui for short) and acuminata ‘Nakai’ (Nakai for short) were used to compare and evaluate the antioxidant activities of hydroethanolic extracts of the fruit using ultrasonic and stirring extraction methods. Dongkui bayberry fruit extract (BFE) prepared using the ultrasonic method exhibited a significantly higher value for the total phenolic content (TPC) and had lower 50% inhibitory concentration (IC50) values of scavenging activities against 1,1-diphenyl-2- picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS), and hydrogen peroxide (H2O2), as well as reducing power compared to the other treatment. The TPC of the BFE was significantly correlated with its DPPH, ABTS, and H2O2 radical-scavenging and reducing power activities. Dongkui BFE at a concentration of 0.25 mg/mL exhibited significantly greater protection of RAW 264.7 mouse macrophages against H2O2-induced damage and lipopolysaccharide (LPS)-stimulated nitric oxide production by macrophages, and it displayed remarkable inhibitory effects compared to the other extracts using the ultrasonic extraction method. Furthermore, compared to the Nakai BFE, macrophages exposed to the Dongkui BFE by the ultrasonic extraction method significantly inhibited LPS-induced production of tumor necrosis factor-α at a concentration of the extract of 0.25 mg/mL. The antioxidant properties and anti-inflammatory and protective effects of BFE prepared by stirring and ultrasonic methods are discussed for the first time in this study.   ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********

scholarly journals Phytochemicals and In-Vitro Antioxidant Activities of Aloe Vera Gel

2020 ◽  
Vol 44 (1) ◽  
pp. 33-41
Author(s):  
Md Nazim Uddin ◽  
Subrata Chandra Roy ◽  
Abdulla All Mamun ◽  
Kanika Mitra ◽  
Md Zahurul Haque ◽  
...  
Keyword(s):  
Gallic Acid ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Aloe Vera ◽  
The Other ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Aloe Vera Gel ◽  
Gallic Acid Equivalent

The phytochemicals (total phenolic, tannin, flavonoid, alkaloid, and saponin) contents in the Aloe vera gel derived from the leaf of Aloe vera (L.) Burm. f. (Synonym Aloe barbadensismiller) were extracted and their antioxidant capacity was studied by Ferric reducing antioxidant power assay (FRAP), by free radical-scavenging capability using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Three different solvents with three different polarities CH3OH, CH3OH: HCl, CH3CH3OH: H2O were used at v/v ratio of 100, 98:2, 70:30, respectively. The acidified methanol solvent extracted the highest amounts of phytochemicals including total phenolic (4.64 mg gallic acid equivalent/g), tannin (3.84 mg tannic acid equivalent/g), alkaloid (662 mg piperine equivalent/g), and saponin (353 mg diosgenin equivalents/g) compared to the other two solvents. Similarly, in the extract with acidified methanol solvent, high level of total antioxidant activity (about 12 mg gallic acid equivalent/g) and scavenging effects expressed as 50% inhibition concentrations (IC50) for DPPH and ABTS assay were determined to be about 61 μg/mL and 371 μg/mL, respectively, which are higher than those with the other two solvents. The gel extract could be used as a potent antioxidant in medicine and food industries. Journal of Bangladesh Academy of Sciences, Vol. 44, No. 1, 33-41, 2020

scholarly journals Nutritional Composition, Antioxidant Activities, and Antiulcer Potential ofLentinus squarrosulus(Mont.) Mycelia Extract

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Nor Adila Mhd Omar ◽  
Noorlidah Abdullah ◽  
Umah Rani Kuppusamy ◽  
Mahmood Ameen Abdulla ◽  
Vikineswary Sabaratnam
Keyword(s):  
Antioxidant Capacity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Water Extract ◽  
Nutritional Composition ◽  
Oral Feeding ◽  
Total Phenolic ◽  
Sprague Dawley ◽  
Total Fat

Water extract ofLentinus squarrosulusmycelia was analysed for nutritional content, antioxidant capacity, and antiulcer ability. The extract contains high protein (57.6 g/100 g) and low total fat (0.5 g/100 g) and is rich in magnesium (0.4 g/100 g), potassium (3.8 g/100 g), vitamins B1(1.42 mg/100 g), and B3(194.29 mg/100 g) with total phenolic content of 39.16 mg/100 g. The cupric reducing antioxidant capacity and 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity of the extract were A450of 0.20 ± 0.03 at 0.5 mg/ml and IC50of 14.29 mg/ml, respectively. Oral feeding ofL. squarrosulusextract (250 mg/kg) offered significant gastric mucosal protection of Sprague-Dawley rats compared to cimetidine (50 mg/kg). The ulcer healing rate of ulcerated rats after 24, 48, and 72 hours of treatment was 82%, 90%, and 100%, respectively. The IL-1β level in the serum and the NF-κB level in the tissues indicate that the healing potential was associated with attenuation of proinflammatory cytokines.

Sign in / Sign up

Export Citation Format

Share Document