scholarly journals The Development of Microscopy for Super-Resolution: Confocal Microscopy, and Image Scanning Microscopy

2021 ◽  
Vol 11 (19) ◽  
pp. 8981
Author(s):  
Colin J. R. Sheppard
Keyword(s):  
Confocal Microscopy ◽  
Biological Samples ◽  
Super Resolution ◽  
Scanning Microscopy ◽  
Optical Methods ◽  
Nonlinear Microscopy ◽  
Structured Illumination ◽  
Basic Principles ◽  
Image Scanning ◽  
Super Resolution Microscopy

Optical methods of super-resolution microscopy, such as confocal microscopy, structured illumination, nonlinear microscopy, and image scanning microscopy are reviewed. These methods avoid strong invasive interaction with a sample, allowing the observation of delicate biological samples. The meaning of resolution and the basic principles and different approaches to superresolution are discussed.

scholarly journals Super-resolution microscopy based on parallel detection

2019 ◽  
Vol 12 (06) ◽  
pp. 1950023
Author(s):  
Zhimin Zhang ◽  
Shaocong Liu ◽  
Liang Xu ◽  
Yubing Han ◽  
Cuifang Kuang ◽  
...  
Keyword(s):  
Cross Correlation ◽  
Signal To Noise Ratio ◽  
3D Structure ◽  
Super Resolution ◽  
Confocal Imaging ◽  
Scanning Microscopy ◽  
Resolution Limit ◽  
Image Scanning ◽  
Parallel Detection ◽  
Super Resolution Microscopy

Image scanning microscopy based on pixel reassignment can improve the confocal resolution limit without losing the image signal-to-noise ratio (SNR) greatly [C. J. R. Sheppard, “Super-resolution in confocal imaging,” Optik 80(2) 53–54 (1988). C. B. Müller, E. Jörg, “Image scanning microscopy, “Phys. Rev. Lett. 104(19) 198101 (2010). C. J. R. Sheppard, S. B. Mehta, R. Heintzmann, “Superresolution by image scanning microscopy using pixel reassignment,” Opt. Lett. 38(15) 2889–2892 (2013)]. Here, we use a tailor-made optical fiber and 19 avalanche photodiodes (APDs) as parallel detectors to upgrade our existing confocal microscopy, termed as parallel-detection super-resolution (PDSR) microscopy. In order to obtain the correct shift value, we use the normalized 2D cross correlation to calculate the shifting value of each image. We characterized our system performance by imaging fluorescence beads and applied this system to observing the 3D structure of biological specimen.

scholarly journals Structured illumination microscopy and image scanning microscopy: a review and comparison of imaging properties

2021 ◽  
Vol 379 (2199) ◽  
Author(s):  
Colin J. R. Sheppard
Keyword(s):  
Spatial Resolution ◽  
Super Resolution ◽  
Scanning Microscopy ◽  
Structured Illumination ◽  
Practical Application ◽  
Structured Illumination Microscopy ◽  
Image Scanning ◽  
Imaging Performance ◽  
Imaging Properties

Structured illumination microscopy and image scanning microscopy are two microscopical tech- niques, rapidly increasing in practical application, that can result in improvement in transverse spatial resolution, and/or improvement in axial imaging performance. The history and principles of these techniques are reviewed, and the imaging properties of the two methods compared. This article is part of the Theo Murphy meeting issue ‘Super-resolution structured illumination microscopy (part 1)’.

Practical aspects of super-resolution optical fluctuation image scanning microscopy (SOFISM)

2020 ◽  
Author(s):  
Adrian Makowski ◽  
Gur Lubin ◽  
Ron Tenne ◽  
Aleksandra Sroda ◽  
Uri Rossman ◽  
...  
Keyword(s):  
Super Resolution ◽  
Scanning Microscopy ◽  
Image Scanning

Coherent super-resolution microscopy via laterally structured illumination

Micron ◽  
2007 ◽  
Vol 38 (2) ◽  
pp. 150-157 ◽  
Author(s):  
Brad Littleton ◽  
Kim Lai ◽  
Dennis Longstaff ◽  
Vassilios Sarafis ◽  
Paul Munroe ◽  
...  
Keyword(s):  
Super Resolution ◽  
Structured Illumination ◽  
Super Resolution Microscopy

scholarly journals Regulated protein stabilization underpins the functional interplay among basal body components in Trypanosoma brucei

2019 ◽  
Vol 295 (3) ◽  
pp. 729-742
Author(s):  
Kieu T. M. Pham ◽  
Ziyin Li
Keyword(s):  
Trypanosoma Brucei ◽  
Basal Body ◽  
Super Resolution ◽  
Specific Protein ◽  
Protein Stabilization ◽  
Structured Illumination ◽  
Human Parasite ◽  
Evolutionarily Conserved ◽  
Body Components ◽  
Super Resolution Microscopy

The basal body in the human parasite Trypanosoma brucei is structurally equivalent to the centriole in animals and functions in the nucleation of axonemal microtubules in the flagellum. T. brucei lacks many evolutionarily conserved centriolar protein homologs and constructs the basal body through unknown mechanisms. Two evolutionarily conserved centriole/basal body cartwheel proteins, TbSAS-6 and TbBLD10, and a trypanosome-specific protein, BBP65, play essential roles in basal body biogenesis in T. brucei, but how they cooperate in the regulation of basal body assembly remains elusive. Here using RNAi, endogenous epitope tagging, immunofluorescence microscopy, and 3D-structured illumination super-resolution microscopy, we identified a new trypanosome-specific protein named BBP164 and found that it has an essential role in basal body biogenesis in T. brucei. Further investigation of the functional interplay among BBP164 and the other three regulators of basal body assembly revealed that BBP164 and BBP65 are interdependent for maintaining their stability and depend on TbSAS-6 and TbBLD10 for their stabilization in the basal body. Additionally, TbSAS-6 and TbBLD10 are independent from each other and from BBP164 and BBP65 for maintaining their stability in the basal body. These findings demonstrate that basal body cartwheel proteins are required for stabilizing other basal body components and uncover that regulation of protein stability is an unusual control mechanism for assembly of the basal body in T. brucei.

scholarly journals Super-resolution microscopy and its applications in neuroscience

2017 ◽  
Vol 10 (05) ◽  
pp. 1730001 ◽  
Author(s):  
Xuecen Wang ◽  
Jiahao Wang ◽  
Xinpei Zhu ◽  
Yao Zheng ◽  
Ke Si ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Super Resolution ◽  
Diffraction Limit ◽  
Biological Science ◽  
Basic Principles ◽  
Suitable Tool ◽  
Subcellular Processes ◽  
Conventional Microscopy ◽  
Super Resolution Microscopy

Optical microscopy promises researchers to see most tiny substances directly. However, the resolution of conventional microscopy is restricted by the diffraction limit. This makes it a challenge to observe subcellular processes happened in nanoscale. The development of super-resolution microscopy provides a solution to this challenge. Here, we briefly review several commonly used super-resolution techniques, explicating their basic principles and applications in biological science, especially in neuroscience. In addition, characteristics and limitations of each technique are compared to provide a guidance for biologists to choose the most suitable tool.

scholarly journals Advances in High-Speed Structured Illumination Microscopy

2021 ◽  
Vol 9 ◽  
Author(s):  
Tianyu Zhao ◽  
Zhaojun Wang ◽  
Tongsheng Chen ◽  
Ming Lei ◽  
Baoli Yao ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
High Speed ◽  
Cell Imaging ◽  
Super Resolution ◽  
Live Cell ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Light Power ◽  
Recent Developments ◽  
Super Resolution Microscopy

Super-resolution microscopy surpasses the diffraction limit to enable the observation of the fine details in sub-cellular structures and their dynamics in diverse biological processes within living cells. Structured illumination microscopy (SIM) uses a relatively low illumination light power compared with other super-resolution microscopies and has great potential to meet the demands of live-cell imaging. However, the imaging acquisition and reconstruction speeds limit its further applications. In this article, recent developments all targeted at improving the overall speed of SIM are reviewed. These comprise both hardware and software improvements, which include a reduction in the number of raw images, GPU acceleration, deep learning and the spatial domain reconstruction. We also discuss the application of these developments in live-cell imaging.

scholarly journals A robust and versatile platform for image scanning microscopy enabling super-resolution FLIM

2019 ◽  
Vol 16 (2) ◽  
pp. 175-178 ◽  
Author(s):  
Marco Castello ◽  
Giorgio Tortarolo ◽  
Mauro Buttafava ◽  
Takahiro Deguchi ◽  
Federica Villa ◽  
...  
Keyword(s):  
Super Resolution ◽  
Scanning Microscopy ◽  
Image Scanning
Sign in / Sign up

Export Citation Format

Share Document