scholarly journals Enhancement of Growth and Paramylon Production of Euglena gracilis by Upcycling of Spent Tomato Byproduct as an Alternative Medium

2021 ◽  
Vol 11 (17) ◽  
pp. 8182
Author(s):  
Sunah Kim ◽  
Riry Wirasnita ◽  
Donghyun Lee ◽  
Jaecheul Yu ◽  
Taeho Lee
Keyword(s):  
Carbon Source ◽  
Organic Carbon ◽  
Euglena Gracilis ◽  
Food Processing ◽  
Environmental Remediation ◽  
Synthetic Medium ◽  
Processing Plant ◽  
Beta Glucan ◽  
Carbon And Nitrogen ◽  
Initial Ph

Euglena gracilis (E. gracilis) accumulates paramylon, an immune-functional beta-glucan that can be used as a functional food. Paramylon production is strongly affected by the organic carbon source and the initial pH conditions. Food processing byproducts have attracted attention for microalgal cultivation because of their low cost and abundance of nutrients, including carbon and nitrogen. We investigated the optimal carbon source and its concentration for efficient paramylon production. A spent tomato byproduct (STB) generated from a tomato processing plant was applied for biomass and paramylon production from E. gracilis with respect to the initial pH condition. The highest paramylon concentration (1.2 g L−1) and content (58.2%) were observed with 15 g L−1 glucose. The biomass production increased when STB was used as compared with that when a synthetic medium was used (1.6-fold higher at pH 3 and 2-fold higher at pH 8). The optimal initial pH was determined according to the maximum production of biomass and paramylon. Upcycling the food processing byproduct, STB, can contribute not only to cost reduction of the biorefinery process using E. gracilis but also to environmental remediation by removing organic carbon and nitrogen from the byproducts.

Conversion of Industrial Wastes to Produce Useful Bio-Organics

1971 ◽  
Vol 6 (1) ◽  
pp. 38-52
Author(s):  
F.G. Peacock ◽  
O. T. Page
Keyword(s):  
Food Processing ◽  
Chromatographic Analysis ◽  
Fungal Growth ◽  
Industrial Wastes ◽  
Processing Plant ◽  
Biological Degradation ◽  
Treated Effluent ◽  
Before And After ◽  
Initial Ph ◽  
Made In

Abstract Studies were conducted on the biological degradation, by the fungus Fusarium moniloforme, of solubles in effluent from food processing plant wastes. Initial pH of the effluent ranged from 10.5 to 11.5. Marked decreases in pH were found to occur during fungal growth. UV spectrophotometric analyses (200–450nm) of this effluent showed a progressive decrease in absorbance over several days of treatment. Starch, a major component of the effluent, was utilized and eliminated by the organism as determined by visual and spectrophotometric IKI tests. Chromatographic analysis before and after treatment indicated a substantial decrease in the number of amino acids whether marketable by-produce were produced by the fungus during this process, examinations of the treated waste are being carried out. IR spectrophotometric determinations of steroids have been made in dry chloroform extracts following controlled fungal growth on primary treated effluent.

Stabilization of Organic Carbon and Nitrogen in Consolidating Benthal Deposits

1985 ◽  
Vol 17 (6-7) ◽  
pp. 929-940 ◽  
Author(s):  
C. W. Bryant ◽  
L. G. Rich
Keyword(s):  
Organic Carbon ◽  
Model Verification ◽  
Volatile Acid ◽  
Free Energies ◽  
Organic Loading ◽  
Carbon And Nitrogen ◽  
Loading Rates ◽  
Degradation Reactions ◽  
The Individual ◽  
Organic Deposits

The objective of this research was to develop and validate a predictive model of the benthal stabilization of organic carbon and nitrogen in deposits of waste activated sludge solids formed at the bottom of an aerated water column, under conditions of continual deposition. A benthal model was developed from a one-dimensional, generalized transport equation and a set of first-order biological reactions. For model verification, depth profiles of the major interstitial carbon and nitrogen components were measured from a set of deposits formed in the laboratory at 20°C and a controlled loading rate. The observed sequence of volatile acid utilization in each benthal deposit was that which would be predicted by the Gibbs free energies of the individual degradation reactions and would be controlled by the reduction in interstitial hydrogen partial pressure with time. Biodegradable solids were solubilized rapidly during the first three weeks of benthal retention, but subsequent solubilization occurred much more slowly. The benthal simulation effectively predicted the dynamics of consolidating, organic deposits. Simulation of organic loading rates up to 250 g BVSS/(m2 day) indicated that the stabilization capacity of benthal deposits was far above the range of organic loading rates currently used in lagoon design.

scholarly journals Isolation of Rhodocyclus gelatinosus from poultry slaughterhouse wastewater

2002 ◽  
Vol 45 (4) ◽  
pp. 445-449 ◽  
Author(s):  
Elisa Helena Giglio Ponsano ◽  
Pedro Magalhães Lacava ◽  
Marcos Franke Pinto
Keyword(s):  
Carbon Source ◽  
Photosynthetic Bacteria ◽  
Synthetic Medium ◽  
Poultry Feed ◽  
Gram Negative ◽  
Slaughterhouse Wastewater ◽  
Rhodocyclus Gelatinosus

Four cultures of photosynthetic bacteria isolated from poultry slaughterhouse wastewater were identified as Rhodocyclus gelatinosus based on the following properties: reddish color of cultures in synthetic medium, presence of motility, slightly curved Gram-negative rods morphology, gelatin liquefying activity, utilization of citrate as carbon source and production of bacteriochlorophyl a and carotenoids of the spirilloxanthin alternative series. R. gelatinosus may represent a source of nutrients and pigments with application in poultry feed.

The Interactions of Carbon and Nitrogen in Liquid Silicon

2014 ◽  
Vol 33 (4) ◽  
pp. 363-368 ◽  
Author(s):  
Halvor Dalaker ◽  
Merete Tangstad
Keyword(s):  
Silicon Nitride ◽  
Carbon Source ◽  
Nitrogen Content ◽  
High Purity ◽  
Liquid Silicon ◽  
Interaction Parameters ◽  
Carbon And Nitrogen ◽  
Temperature Range ◽  
High Purity Silicon

AbstractThe interactions between carbon and nitrogen in liquid silicon have been studied experimentally. High purity silicon was melted in silicon nitride crucibles under an Ar atmosphere with a graphite slab inserted in the crucible prior to melting as a carbon source. The system was thus simultaneously equilibrated with Si3N4 and SiC. Samples were extracted in the temperature range 1695–1798 K and analyzed using Leco.It was observed that the simultaneous saturation of nitrogen and carbon caused a significant increase in the solubilities of both elements. The interaction parameters were derived as The solubility of carbon in liquid silicon as a function of temperature and nitrogen content was found to follow: And the solubility of nitrogen in liquid silicon found to follow:

Soil Organic Carbon and Nitrogen Variations with Vegetation Succession in Passively Restored Freshwater Wetlands

Wetlands ◽  
2021 ◽  
Vol 41 (1) ◽  
Author(s):  
Yu An ◽  
Yang Gao ◽  
Xiaohui Liu ◽  
Shouzheng Tong ◽  
Bo Liu ◽  
...  
Keyword(s):  
Organic Carbon ◽  
Soil Organic Carbon ◽  
Vegetation Succession ◽  
Freshwater Wetlands ◽  
Carbon And Nitrogen

scholarly journals Biosurfactant from endophytic Bacillus pumilus 2A: physicochemical characterization, production and optimization and potential for plant growth promotion

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Olga Marchut-Mikołajczyk ◽  
Piotr Drożdżyński ◽  
Arkadiusz Polewczyk ◽  
Wojciech Smułek ◽  
Tadeusz Antczak
Keyword(s):  
Phaseolus Vulgaris ◽  
Carbon Source ◽  
Plant Growth ◽  
Taguchi Method ◽  
Beta Vulgaris ◽  
Food Processing ◽  
Bacillus Pumilus ◽  
Brewer’S Spent Grain ◽  
Spent Grain ◽  
By Products

Abstract Background Microbial surfactants called biosurfactants, thanks to their high biodegradability, low toxicity and stability can be used not only in bioremediation and oil processing, but also in the food and cosmetic industries, and even in medicine. However, the high production costs of microbial surfactants and low efficiency limit their large-scale production. This requires optimization of management conditions, including the possibility of using waste as a carbon source, such as food processing by-products. This papers describes the production and characterization of the biosurfactant obtained from the endophytic bacterial strain Bacillus pumilus 2A grown on various by-products of food processing and its potential applications in supporting plant growth. Four different carbon and nitrogen sources, pH, inoculum concentration and temperature were optimized within Taguchi method. Results Optimization of bioprocess within Taguchi method and experimental analysis revealed that the optimal conditions for biosurfactant production were brewer’s spent grain (5% w/v), ammonium nitrate (1% w/v), pH of 6, 5% of inoculum, and temperature at 30 °C, leading to 6.8 g/L of biosurfactant. Based on gas chromatography–mass spectrometry and Fourier transform infrared spectroscopy analysis produced biosurfactant was determined as glycolipid. Obtained biosurfactant has shown high and long term thermostability, surface tension of 47.7 mN/m, oil displacement of 8 cm and the emulsion index of 69.11%. The examined glycolipid, used in a concentration of 0.2% significantly enhanced growth of Phaseolus vulgaris L. (bean), Raphanus L. (radish), Beta vulgaris L. (beetroot). Conclusions The endophytic Bacillus pumilus 2A produce glycolipid biosurfactant with high and long tem thermostability, what makes it useful for many purposes including food processing. The use of brewer’s spent grain as the sole carbon source makes the production of biosurfactants profitable, and from an environmental point of view, it is an environmentally friendly way to remove food processing by products. Glycolipid produced by endophytic Bacillus pumilus 2A significantly improve growth of Phaseolus vulgaris L. (bean), Raphanus L. (radish), Beta vulgaris L. (beetroot). Obtained results provide new insight to the possible use of glycolipids as plant growth promoting agents.

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