scholarly journals Proteomic and Metabolomic Analyses of Maggots in Porcine Corpses for Post-Mortem Interval Estimation

2021 ◽  
Vol 11 (17) ◽  
pp. 7885
Author(s):  
Jeong-Hun Mok ◽  
Minjoong Joo ◽  
Van-An Duong ◽  
Seonghyeon Cho ◽  
Jong-Moon Park ◽  
...  
Keyword(s):  
Interval Estimation ◽  
Guanosine Monophosphate ◽  
Potential Candidate ◽  
Post Mortem ◽  
Post Mortem Interval ◽  
Protein Pellet ◽  
Proteome Discoverer ◽  
Liquid Chromatography Tandem Mass ◽  
Metabolome Profiling ◽  
Pig Carcasses

Post-mortem interval (PMI) estimation is a critical task in forensic science. In this study, we used maggots collected from pig carcasses and applied an integrated proteomics and metabolomics approach to determine potential candidate substances for the estimation of PMI. After methanol precipitation, the supernatant containing metabolites and the protein pellet were separated and subjected to metabolomic and proteomic analyses using liquid chromatography-tandem mass spectrometry (LC-MS/MS). MS/MS data were analyzed for identification and quantification using Proteome Discoverer and Compound Discoverer software. A total of 573 metabolites and more than 800 porcine proteins were identified in maggots. This is the first dataset of proteins and metabolites in maggots collected from porcine carcasses. In this study, guanosine monophosphate, xanthine, inosine, adenosine, and guanine were detected with a similar tendency to increase during early days of maggot development and then decreased gradually. We broadly profiled various biomolecules through analysis in the spot of incident. Especially, we confirmed that proteome and metabolome profiling could be performed directly and indirectly.

A novel method for post-mortem interval estimation based on tissue nano-mechanics

2019 ◽  
Vol 133 (4) ◽  
pp. 1133-1139 ◽  
Author(s):  
Fabio De-Giorgio ◽  
Matteo Nardini ◽  
Federica Foti ◽  
Eleonora Minelli ◽  
Massimiliano Papi ◽  
...  
Keyword(s):  
Interval Estimation ◽  
Post Mortem ◽  
Post Mortem Interval ◽  
Novel Method

Post-mortem interval estimation based on insect evidence in a quasi-indoor habitat

2019 ◽  
Vol 59 (1) ◽  
pp. 109-115 ◽  
Author(s):  
Szymon Matuszewski ◽  
Anna Mądra-Bielewicz
Keyword(s):  
Interval Estimation ◽  
Post Mortem ◽  
Post Mortem Interval

scholarly journals Characterization of necrophagus entomofauna in a typical agricultural area in Emilia-Romagna region (Northern Italy)

Entomologia ◽  
2014 ◽  
Author(s):  
M. Calzolari ◽  
F. Defilippo ◽  
G. Zani ◽  
M. Colombo ◽  
M. Dottori
Keyword(s):  
Forensic Entomology ◽  
Interval Estimation ◽  
Northern Italy ◽  
Agricultural Area ◽  
Post Mortem Interval ◽  
Po Valley ◽  
Flying Insects ◽  
Emilia Romagna Region ◽  
Pig Carcasses

This study presents a checklist of Dipterans and Coleopterans accountable for carrion decay in the Po Valley (Italy), a contribution to the Forensic Entomology knowledge in Italy. Insects colonizing two pig carcasses in an agricultural area in Mezzani municipality (Parma, Northern Italy) were sampled by pitfall traps and original Malaise-like traps, which allowed the sampling of a very relevant number of flying insects. A checklist of 57 taxa was obtained, of which 26 were considered of forensic importance. For the latter the arrival time of adult specimens on the carcasses was recorded, as an important parameter in minimum post mortem interval estimation. Dipterans (6141 specimens) were the most common insects; the fastest specimens to detect and colonize the carcass belonged to the Calliphoridae family, while Fanniidae and Muscidae infested the carrion until completion of the skeletal stage. Coleopterans appeared later (308 adult specimens and 114 larvae were captured). Staphylinidae, Dermestidae, and Histeridae were the most common coleopterans sampled in this study.

scholarly journals The development of a post-mortem interval estimation for human remains found on land in the Netherlands

2017 ◽  
Vol 132 (3) ◽  
pp. 863-873 ◽  
Author(s):  
H. T. Gelderman ◽  
L. Boer ◽  
T. Naujocks ◽  
A. C. M. IJzermans ◽  
W. L. J. M. Duijst
Keyword(s):  
The Netherlands ◽  
Interval Estimation ◽  
Human Remains ◽  
Post Mortem ◽  
Post Mortem Interval

scholarly journals Post-mortem Interval Estimation in a Forensic Case with Two Predatory Species: Chrysomya albiceps and Synthesiomyia nudiseta

2021 ◽  
Vol 6 (1(Special)) ◽  
pp. 148
Author(s):  
Tania Ivorra ◽  
Anabel Martínez-Sánchez ◽  
Salvador Giner Alberola
Keyword(s):  
Interval Estimation ◽  
Post Mortem ◽  
Degree Days ◽  
Se Spain ◽  
Predatory Behaviour ◽  
Chrysomya Albiceps ◽  
Post Mortem Interval ◽  
Forensic Case ◽  
The Town ◽  
Accumulated Degree Days

A forensic case in which two facultative predatory species were found together on a human corpse is presented in this work. Second and third instar larvae of Synthesiomyia nudiseta (van der Wulp, 1883) (Diptera: Muscidae) and Chrysomya albiceps (Wiedemann, 1819) (Diptera: Calliphoridae) were collected from the dead body of a man on 14th November 2019, but puparia in the autopsy pictures were observed after, but not collected. The corpse was found on the terrace of the third floor of a building located in the town of Elche (SE Spain), and the pathologist confirmed the death as natural. The minimum post-mortem interval was estimated by the time to reach the length of the collected larvae or to reach the pupal developmental stage (in days and accumulated degree-days). In accordance with the influence of the predatory behaviour of each of these species on the developmental data of the other, as described in the existing literature, the effect of a hypothetical competition between both species on post-mortem interval was discussed.

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