scholarly journals Evaluation of Anti-Obesity Activity of an Herbal Formulation (F2) in DIO Mice Model and Validation of UPLC-DAD Method for Quality Control

2021 ◽  
Vol 11 (16) ◽  
pp. 7404
Author(s):  
Prakash Raj Pandeya ◽  
Kyung-Hee Lee ◽  
Ramakanta Lamichhane ◽  
Gopal Lamichhane ◽  
Amrit Poudel ◽  
...  
Keyword(s):  
Quality Control ◽  
Serum Lipid ◽  
Body Weight Gain ◽  
Histological Evaluation ◽  
Control Group ◽  
Obese Mice ◽  
Mice Model ◽  
Curative Therapy ◽  
Triglyceride Accumulation ◽  
Food Efficiency Ratio

Obesity is considered a chronic metabolic disorder that can be associated with multiple medical complications. Currently, there is no or limited curative therapy for obesity. This study focused on the assessment of anti-obesity activity and UPLC standardization of a polyherbal formulation (F2). An anti-obesity activity was investigated using the diet-induced obese (DIO) mice model, where obesity was developed in C57BL/6J mice by providing a high-fat diet (HFD) for five weeks without treating drugs. After the successful development of obesity, the obese mice were treated with F2 for seven weeks with continuing HFD feeding. The major obesity-related parameters such as body weight gain, food efficiency ratio, serum lipid profile, and white adipose tissue (WAT) mass were found to be significantly reduced in F2 treated obese mice. These results were supported by the down-regulation of specific adipogenic transcription factors (PPARγ, SREBP-1c, and ap2) in epididymal WAT. Histological evaluation of liver and WAT also revealed reduced fat deposition in the tissues by F2 compared to the HFD control group. The overall observations indicated that the F2 exhibited pronounced obesity-controlling activity through the inhibition of adipocyte differentiation and triglyceride accumulation in the tissues, and serum lipid depletion. In addition, F2 ameliorated obesity-induced insulin resistance. Furthermore, the UPLC-DAD method for quality control of F2 was validated and standardized using five reference compounds: astragalin, ellagic acid, fisetin, fustin, and sulfuretin.

scholarly journals Chronic photoperiodic manipulation induced chronodisruption upregulates HSP60 during early pro-atherogenic remodeling in thoracic aorta of C57BL/6J mice

2021 ◽  
Vol 82 (1) ◽  
Author(s):  
Kavita Shirsath ◽  
Apeksha Joshi ◽  
Aliasgar Vohra ◽  
Ranjitsinh Devkar
Keyword(s):  
Body Weight ◽  
Thoracic Aorta ◽  
Serum Lipid ◽  
Body Weight Gain ◽  
Hdl Cholesterol ◽  
Circadian Disruption ◽  
Control Group ◽  
Chow Diet ◽  
Heat Shock Protein 60 ◽  
Lipid Parameters

Abstract Background Circadian disruption is often associated with aggravation of atherosclerosis; however, the pathophysiological mechanisms underlying atherogenic initiation in normolipidemic diet remains unclear. Most of the studies done for understanding circadian disruption induced atherosclerosis have been carried out in murine model of hyperlipidemia induced atherosclerosis. The present study investigates pro-atherogenic events in response to chronic photoperiodic manipulation induced chronodisruption (PMCD) in C57BL/6J mice fed with laboratory chow diet. Results The results were compared with atherogenic initiation induced by high fat high fructose (HFHF) diet. The combined effects of HFHF and PMCD on atherogenic initiation were also investigated for possible synergy of both variants. The HFHF and HFHF+PMCD groups recorded increments in body weight gains and serum lipid parameters (TC, TG, LDL-cholesterol, VLDL) and a decrement in HDL-cholesterol as compared to the control group. However, PMCD group recorded body weight gain similar to that of the control group, but the serum lipid parameters (TG and VLDL) were significantly elevated and the HDL levels were lowered. However, prominent hypertrophic remodeling, higher collagen deposition, and elastin derangement, along with endothelial dysfunction, its activation, and macrophage infiltration, were observed in thoracic aorta of all the three experimental groups. But the mRNA and immunoblots of heat shock protein 60 (HSP60) in thoracic aorta was found to be maximum in PMCD followed by HFHF and HFHF+PMCD groups. Conclusion Laboratory chow feeding coupled with photoperiodic manipulation mediated chronodisruption overexpress HSP60 that in turn plays a central role in PMCD mediated pro-atherogenic remodeling in thoracic aorta of C57BL/6J mice.

scholarly journals ANTI-OBESITY EFFECT OF CANNABIS SATIVA SEED FLOUR FROM KHLALFA OF TAOUNATE REGION (NORTHERN OF MOROCCO) IN HIGH CALORIC DIET-INDUCED OBESE MICE

2020 ◽  
pp. 139-144
Author(s):  
MOUNA BOUARFA ◽  
ZOUHAIR SOUIRTI ◽  
PIERFRANCESCO CERRUTI ◽  
MAURIZIO AVELLA ◽  
ABDELLAH FARAH ◽  
...  
Keyword(s):  
Cannabis Sativa ◽  
Body Weight Gain ◽  
Hdl Cholesterol ◽  
Serum Glucose ◽  
Normal Diet ◽  
Control Group ◽  
Obese Mice ◽  
Seed Flour ◽  
Diet Control ◽  
High Caloric Diet

Objective: The objective of the present study was to evaluate the anti-obesity activity of Cannabis sativa seed Flour (CSSF) on obese mice. Methods: The experiment process was carried out in 8 weeks. In this time, three groups of animals were adopted: High caloric diet control group (HCD), group treated with 100 mg/kg of CSSF, group treated with 200 mg/kg of CSSF, and normal diet control group (ND). Then, the histopathological and biochemical parameters analysis has been realized at the end of the experiment. Results: As a result, body weight gain, epididymal, and perirenal adipose tissue decreased in the CSSF (Cannabis sativa seed Flour) group in both doses (100 mg/kg and 200 mg/kg) compared to the HCD. CSSF lowered serum glucose level elevated by HCD. For lipid profiles, total cholesterol (TC), HDL-cholesterol, LDL-cholesterol, and triglycerides (TG) significantly decreased in the CSSF group. The histopathological results of this study showed a regenerative effect of CSSF on the kidney and liver tissues. Conclusion: It is concluded from the present study that CSSF can be used as a natural anti-obesity agent.

scholarly journals Anti-Obese Effect of Cinnamon Extracts Dietary Supplementation on Serum Lipids and Body Weight Gain in High-Fat-Diet Induced Obese Mice Model

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 1236-1236
Author(s):  
Joohee Oh ◽  
Hyun-Sook !Kim
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
High Fat Diet ◽  
Body Weight Gain ◽  
Diet Group ◽  
Normal Diet ◽  
Obese Mice ◽  
Mice Model ◽  
High Fat ◽  
Final Body Weight

Abstract Objectives Cinnamon is one of the oldest spices widely used in traditional medicine and also currently used by people all around the world. Cinnamon has been known for modulating metabolic disorders by regulating insulin sensitivity. The aim of this study was to investigate the anti-obese effects of cinnamon extracts in high-fat-diet induced obese mice model. Methods After a week of adaptation period, the 6-week-old male C57BL/6J mice were randomly divided into 4 groups (n = 11 for each group) of the normal diet group (ND), the high-fat-diet group (HF), the normal diet with 1% cinnamon extracts (NC), and the high-fat diet with 1% cinnamon extracts (HC). All groups were treated for 14 weeks. Results In final body weight and body weight gain, NC group was significantly lower than ND group and HC group was significantly lower than HF group (P = 0.000). In serum TG (Triglyceride) levels and TC (Total cholesterol) levels, NC group showed significantly decreased level compared to that of ND group and HC group represented significantly decreased level compared to that of HF group (P = 0.000). Conclusions The present data showed NC group and HC group showed lower final body weight and body weight gain than ND group and HF group. Also, NC group and HC group showed the decreased level of TG (Triglyceride) and TC (Total cholesterol) compared to ND group and HF group. The further indicators of insulin-related factors are in progress. Funding Sources This study received no external funding.

scholarly journals Single Oral Acute Toxicity of Banhasasim-Tang and Its Antiobesity Effect on Diet-Induced Obese Mice and 3T3-L1 Adipocytes

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Sae-Rom Yoo ◽  
Soo-Jin Jeong ◽  
Mee-young Lee ◽  
Hyeun-Kyoo Shin ◽  
Chang-Seob Seo ◽  
...  
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Acute Toxicity ◽  
Body Weight Gain ◽  
Clinical Signs ◽  
The Body ◽  
Dependent Manner ◽  
Obese Mice ◽  
Triglyceride Accumulation ◽  
Sd Rats

We had tested antiobesity effect of 52 traditional herbal formulas in 3T3-L1 adipocyte, and Banhasasim-tang (BHSST) was chosen as one of the effective medications to inhibit triglyceride accumulation. We investigated the antiobesity effect of BHSST on 3T3-L1 adipocytes and high-fat diet- (HFD-) induced obese mice. In addition, we evaluated the acute toxicity of BHSST in Sprague Dawley (SD) rats. Differentiated 3T3-L1 cells were treated with various concentrations of BHSST for 8 days. Accumulated triglyceride level and the expressions of adipogenesis-related genes and proteins were subsequently investigated. To evaluate the single oral toxicity of BHSST, the SD rats of each sex were administered a single dose (5000 mg/kg) of BHSST via oral gavage; the control group received vehicle only. After a single administration, the mortality, clinical signs, gross findings, and body weight were monitored for 15 days. Male C57BL/6J mice were fed HFD for 4 weeks to induce obesity and randomly received 50 mg/kg of Orlistat (n=12, OR), 200 mg/kg of BHSST (n=12, B200), and 1000 mg/kg of BHSST (n=12, B1000) for another 8 weeks. BHSST suppressed the triglyceride contents and lipid accumulation in a dose-dependent manner in 3T3-L1 adipocytes. BHSST also downregulated the adipogenesis-related gene levels and protein expression compared with those in undifferentiated adipocytes. In a single oral dose toxicity study, there was no adverse effect on mortality, clinical signs, body weight changes, and gross findings in the treatment group. HFD-fed mice treated with BHSST showed significantly reduced body weight gain, food efficiency ratio, and white adipose tissue weight. The medial lethal dose (LD50) of BHSST was 5000 mg/kg/day body weight for each sex in the rats. BHSST decreased the body weight gain in HFD-fed obese mice and inhibited triglyceride accumulation via a cascade of multiple factors at the mRNA and protein levels in 3T3-L1 adipocytes.

Adipose-derived Mesenchymal Stem Cells from Obese Mice Prevent Body Weight Gain and Hyperglycemia

2020 ◽  
Author(s):  
Yicheng Qi ◽  
Wen Liu ◽  
Xiangsheng Wang ◽  
Wei Liu ◽  
Jing Ma ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Body Weight ◽  
Weight Gain ◽  
Body Weight Gain ◽  
Disease Status ◽  
Obese Mice ◽  
Mice Model ◽  
Glucose Levels ◽  
Blood Glucose Levels

Abstract Disease status always affects mesenchymal stem cells (MSCs) function which limits their therapeutical effects. This study aims to evaluate the effects of adipose-derived MSCs (ADSCs) on body weight and glucose homeostasis in obese mice model. After 10 weeks high-fat diet feeding, mice were injected with either phosphate buffered saline (PBS), ADSCs from normal control mice (N-ADSCs) or ADSCs from obese mice (O-ADSCs). Mice fed with chow-fat diet were injected with PBS served as normal controls. Obese mice received O-ADSCs showed slower body weight gain, better blood glucose levels and prevented glucose intolerance deterioration. The inguinal fat weights were reduced in mice receiving O-ADSCs compared to other obese groups, may as the increased lipolysis of inguinal fat. Moreover, O-ADSCs improved insulin signaling molecule expression in muscle. These data reveal that obesity derived autologous ADSCs infusion is an effective treatment for obesity and hyperglycemia.

Gut carbohydrate metabolism instead of fat metabolism regulated by gut microbes mediates high-fat diet-induced obesity

2014 ◽  
Vol 5 (3) ◽  
pp. 335-344 ◽  
Author(s):  
M. Li ◽  
D. Gu ◽  
N. Xu ◽  
F. Lei ◽  
L. Du ◽  
...  
Keyword(s):  
Weight Gain ◽  
Carbohydrate Metabolism ◽  
Normal Control ◽  
High Fat Diet ◽  
Body Weight Gain ◽  
Control Group ◽  
Obese Mice ◽  
High Fat ◽  
Gut Microbes ◽  
Diet Induced Obesity

The aim of this study was to investigate the mechanisms underlying the involvement of gut microbes in body weight gain of high-fat diet-fed obesity-prone (obese) and obesity-resistant (lean) mice. C57BL/6 mice were grouped into an obese group, a lean group and a normal control group. Both obese and lean mice were fed a high-fat diet while normal control mice were fed a normal diet; they were observed for six weeks. The results showed that lean mice had lower serum lipid levels, body fat and weight gain than obese mice. The ATPase, succinate dehydrogenase and malate dehydrogenase activities in liver as well as oxygen expenditure and rectal temperature of lean mice were significantly lower than in obese mice. As compared with obese mice, the absorption of intestinal carbohydrates but not of fats or proteins was significantly attenuated in lean mice. Furthermore, 16S rRNA abundances of faecal Firmicutes and Bacteroidetes were significantly reduced in lean mice. In addition, faecal β-D-galactosidase activity and short chain fatty acid levels were significantly decreased in lean mice. Expressions of peroxisome proliferator-activated receptor gamma 2 and CCAAT/enhancer binding protein-β in visceral adipose tissues were significantly downregulated in lean mice as compared with obese mice. Resistance to dyslipidaemia and high-fat diet-induced obesity was mediated by ineffective absorption of intestinal carbohydrates but not of fats or proteins, probably through reducing gut Bacteroidetes and Firmicutes contents and lowering of gut carbohydrate metabolism. The regulation of intestinal carbohydrates instead of fat absorption by gut microbes might be a potential treatment strategy for high-fat diet-induced obesity.

scholarly journals 18KHT01, a Potent Anti-Obesity Polyherbal Formulation

2021 ◽  
Vol 12 ◽  
Author(s):  
Prakash Raj Pandeya ◽  
Ramakanta Lamichhane ◽  
Gopal Lamichhane ◽  
Kyung-Hee Lee ◽  
Hyeong Kyu Lee ◽  
...  
Keyword(s):  
Fasting Blood Glucose ◽  
Histological Evaluation ◽  
Citrus Limon ◽  
Herbal Therapy ◽  
Mice Model ◽  
Quercus Acutissima ◽  
Polyherbal Formulation ◽  
Food Efficiency Ratio

Obesity is a life-threatening metabolic disorder necessitating urgent development of safe and effective therapy. Currently, limited such therapeutic measures are available for obesity. The present study was designed to develop a novel, safe and effective herbal therapy for the management of obesity. A polyherbal formulation (18KHT01) was developed by homogeneously mixing a specific proportion of crude Quercus acutissima (acorn jelly powder), Camellia sinensis (dry leaf buds), and Geranium thunbergii (dry aerial part) along with Citrus limon (fruit juice). Synergistic antioxidant, antiadipogenic, and anti-obesity activities were evaluated by in vitro as well as in vivo studies. In vitro experiments revealed strong synergistic antioxidant and anti-adipogenic activities of 18KHT01. Molecular assessment of 18KHT01 showed significant down-regulation of vital adipogenic factors such as PPARγ, C/EBPα, aP2, SREBP-1c, FAS, and LPL. Based on the results of the preliminary toxicity study, 75 and 150 mg/kg, twice daily doses of 18KHT01 were administered to evaluate anti-obesity activity in diet-induced obese (DIO) C57BL/6J mice model. The major obesity-related parameters such as body weight, weight gain, food efficiency ratio, as well as serum lipid profile were significantly reduced by 18KHT01 with potential synergism. Also, the high-fat diet-induced insulin resistance was suggestively alleviated by the formulation, and thus ameliorated fasting blood glucose. Histological evaluation of liver and white adipose tissue revealed that the significant reduction of fat depositions and thus reduction of these tissue weights. Synergy evaluation experiments exhibited that the 18KHT01 offered strong synergism by improving efficacy and reducing the toxicity of its ingredients. Overall results evidenced the 18KHT01 as a safe and potent anti-obesity herbal therapy.

L-Ascorbic Acid Addition to Chitosan Reduces Body Weight in Overweight Women

2014 ◽  
Vol 84 (1-2) ◽  
pp. 5-11 ◽  
Author(s):  
Eun Y. Jung ◽  
Sung C. Jun ◽  
Un J. Chang ◽  
Hyung J. Suh
Keyword(s):  
Ascorbic Acid ◽  
Body Weight ◽  
Fat Body ◽  
Body Weight Gain ◽  
Skinfold Thickness ◽  
The Body ◽  
Control Group ◽  
Percentage Body Fat ◽  
Overweight Women ◽  
Body Weights

Previously, we have found that the addition of L-ascorbic acid to chitosan enhanced the reduction in body weight gain in guinea pigs fed a high-fat diet. We hypothesized that the addition of L-ascorbic acid to chitosan would accelerate the reduction of body weight in humans, similar to the animal model. Overweight subjects administered chitosan with or without L-ascorbic acid for 8 weeks, were assigned to three groups: Control group (N = 26, placebo, vehicle only), Chito group (N = 27, 3 g/day chitosan), and Chito-vita group (N = 27, 3 g/day chitosan plus 2 g/day L-ascorbic acid). The body weights and body mass index (BMI) of the Chito and Chito-vita groups decreased significantly (p < 0.05) compared to the Control group. The BMI of the Chito-vita group decreased significantly compared to the Chito group (Chito: -1.0 kg/m2 vs. Chito-vita: -1.6 kg/m2, p < 0.05). The results showed that the chitosan enhanced reduction of body weight and BMI was accentuated by the addition of L-ascorbic acid. The fat mass, percentage body fat, body circumference, and skinfold thickness in the Chito and Chito-vita groups decreased more than the Control group; however, these parameters were not significantly different between the three groups. Chitosan combined with L-ascorbic acid may be useful for controlling body weight.

202-OR: Comparing Glycemic Control of Youth with T1D in NICH to Referred Youth: Quality Control (Group)

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 202-OR
Author(s):  
DAVID V. WAGNER ◽  
JAKE MAHON ◽  
CELESTE JENISCH ◽  
SAMANTHA A. BARRY ◽  
HANNAH LUZOD ◽  
...  
Keyword(s):  
Quality Control ◽  
Glycemic Control ◽  
Control Group

Effect of HylocereusPolyrhizus Rind Extract Toward Interleukin-1β, Vascular Endothelial Growth Factor Expression, Endometriosis Implant Area

2017 ◽  
Vol 9 (08) ◽  
Author(s):  
YanuarEka P. ◽  
Hendy Hendarto ◽  
Widjiati .
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Treatment Group ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Vascular Endothelial ◽  
Mice Model ◽  
Endothelial Growth Factor ◽  
Fruit Rind

Retrograde menstruation lead to I Kappa B Kinase (IKK) fosforilation in peritoneum macrophage and cause secretion of proinflammatory cytokine interleukin1β then stimulate endometriosis cell to produce Vascular Endothelial Growth Factor which lead to increasing of endometriosis lession seen as endometriosis implant area. Cytokine secretion was inhibited through prevention of NF-κB activation by dragon red fruit rind extract (Hylocereuspolyrhizus). The aim of this reserach is to know the effect of dragon red fuit rind extract with 0,25; 0,5; and 1 mg/g bodyweight dosage toward IL-1β, VEGF expression and implant area in endometriosis mice model. The design of this experiment was randomized post test only control group design.Endometrios mice model were made in 14 days and split into two group, positive control group and treatment group after two week negative control group and postive control group were given Na-CMC 0,5% solution consequetively, and treatment group were given dragon red fruit extract with different dosage. Signification number for IL-1β is p>0,05, signification number for VEGF is p>0,05, and implant area signification number is p>0,05. Administration of dragon red fruit rind extract can decrease IL-1β, VEGF, and implant area.

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