scholarly journals Healing Capacity of Bone Surrounding Biofilm-Infected and Non-Infected Gutta-Percha: A Study of Rat Calvaria

2021 ◽  
Vol 11 (15) ◽  
pp. 6710
Author(s):  
Daniel Moreinos ◽  
Ronald Wigler ◽  
Yuval Geffen ◽  
Sharon Akrish ◽  
Shaul Lin

This paper aims to evaluate the healing capacity of bony lesions around biofilm-infected and non-infected gutta-percha (GP) points. Bony defects were created in the calvaria of 28 Wistar rats. The rats were divided into three groups: Group 1—Implantation of infected GP particles in the bony defect; Group 2—Positive control implantation of non-infected GP particles in the bony defect; and Group 3—Negative control, in which no GP particles were implanted. The biofilm consisted of three strains of bacteria: Enterococcus faecalis, Streptococcus sanguis, and Porphyromonas gingivalis. The animals were sacrificed 60 days postoperation, and histological assessments were performed. In Group 1, the biofilm-infected group, we observed a mild foreign body reaction with a few inflammatory cells adjacent to the capsule and a newly woven bone matrix surrounded by osteoblasts and mature bone. In Group 2, the non-infected GP particles group, minimal inflammatory cell reactions were observed in the adjacent tissue, and a newly woven bone matrix was surrounded by osteoblasts. This study shows that bone healing is possible around both sterile and infected GP points. This contradicts the claim that some root canal treatments fail because of non-microbial factors, including extruded root canal filling materials, which may cause foreign body reactions. The healing observed suggests that overextension should not be considered an indication for endodontic surgery.

2014 ◽  
Vol 8 (1) ◽  
pp. 184-187 ◽  
Author(s):  
Hamed Homayouni ◽  
Nima Moradi Majd ◽  
Heidar Zohrehei ◽  
Behrad Mosavari ◽  
Mamak Adel ◽  
...  

Objectives: The aim of this study was to evaluate the effect of the precipitate that was formed by combining Sodium Hypochlorite (NaOCl) and Chlorhexidine Gluconate (CHX) on the sealing ability of root canal obturation materials. Materials and Methods: The fluid filtration method was conducted on a total of 100 roots. Samples were randomly divided into two control (n=5) and three experimental groups (n=30). The samples in group 1 were irrigated with 1.5 mL of 2.5% NaOCl, and then the smear layers of the teeth were removed by 17% EDTA, while the specimens of group 2 were irrigated by 1.5 mL of 2.5% NaOCl and 1.5 mL of 2% CHX; after the smear layer removal, a final flush with 1.5 mL of 2.5% NaOCl was performed. The samples of group 3 were irrigated the same as group 1 but after the smear layer removal canals were irrigated again with 1.5 mL of 2.5% NaOCl and then a final flush with 1.5 mL of 2% CHX was performed. Teeth were obturated with gutta-percha and AH26 sealer and after seven days, microleakage was evaluated by the fluid filtration technique. The results were analyzed by the ANOVA and Tukey's test. Results: The samples in group 3 had significantly greater microleakage compared to teeth in group 1, 2 (p<0.05), and the specimens in group 1 showed significantly less amount of microleakage than samples in group 2, 3 (p<0.05). Conclusion: The presence of the precipitate that is formed due to interaction between NaOCl and CHX has negative effect on the sealing ability of gutta-percha and AH26 sealer.


2014 ◽  
Vol 08 (01) ◽  
pp. 032-037 ◽  
Author(s):  
Ali Keles ◽  
Fuat Ahmetoglu ◽  
Mevlut S. Ocak ◽  
Burak Dayi ◽  
Alperen Bozkurt ◽  
...  

ABSTRACT Objectives: The aim of this study was to evaluate the sealing abilities of three different gutta-percha techniques in experimentally defective roots (EDR) and non-defective roots (NR). Materials and Methods: Sixty canine teeth were divided into six groups of ten; Group 1, NR + cold lateral condensation (CLC); Group 2, EDR + LC; Group 3, NR + BeeFill; Group 4, EDR + BeeFill; Group 5, NR + Thermafil; and Group 6, EDR + Thermafil. Apical leakage was measured using a computerized fluid filtration meter with a laser system. Results: Statistical analysis revealed that the CLC demonstrated more microleakage in the EDR than in the NR (P < 0.01). Thermafil demonstrated more microleakage in the NR than in the EDR (P < 0.01). No statistically significant differences were found between the BeeFill groups (P > 0.05). Conclusions: The results of this study indicate that internal resorptive cavities can affect the apical sealing properties of different root canal filling techniques, with Thermafil ensuring the lowest apical microleakage.


2013 ◽  
Vol 14 (3) ◽  
pp. 440-444 ◽  
Author(s):  
Jyoti Sachdeva ◽  
Chetan R Patil ◽  
Venugopal Thangala ◽  
Pabbati Ravi Kumar ◽  
Akash Krishna

ABSTRACT Aim To evaluate and compare the efficacy, cleaning ability of hand and two rotary systems in root canal retreatment. Methodology Sixty extracted premolars were retreated with following systems: Group 1—ProTaper Universal retreatment files, Group 2—ProFile system, Group 3—H-file. Specimens were split longitudinally and amount of remaining gutta-percha on the canal walls was assessed using direct visual scoring with the aid of stereomicroscope. Results were statistically analyzed using ANOVA test. Results Completely clean root canal walls were not achieved with any of the techniques investigated. However, all three systems proved to be effective for gutta-percha removal. Significant difference was found between ProTaper universal retreatment file and H-file, and also between ProFile and H-file. Conclusion Under the conditions of the present study, ProTaper Universal retreatment files left significantly less guttapercha and sealer than ProFile and H-file. Clinical significance Rotary systems in combination with gutta-percha solvents can perform superiorly as compared to the time tested traditional hand instrumentation in root canal retreatment. How to cite this article Shivanand S, Patil CR, Thangala V, Kumar PR, Sachdeva J, Krishna A. To Evaluate and Compare the Efficacy, Cleaning Ability of Hand and Two Rotary Systems in Root Canal Retreatment. J Contemp Dent Pract 2013;14(3):440-444.


2021 ◽  
Vol 37 ◽  
pp. e37004
Author(s):  
Matheus Albino Souza ◽  
João Paulo De Carli ◽  
Fernando Tolfo Rodrigues ◽  
Larissa Piuco ◽  
Karolina Frick Bischoff ◽  
...  

The aim of this study was to evaluate apical transportation and apical root canal sealing after root canal filling in human teeth prepared with MTwo® Rotary System with and without apical foramen enlargement. Twenty mandibular premolars were divided into two groups (n=10). Group 1 had root canals prepared 1mm beyond the apical foramen. Group 2 had root canals prepared 1mm below the root canal length. After chemo-mechanical preparation, samples were submitted to scanning electronic microscopy. Apical foramen images had 75x magnification at standardized positions, allowing measurements from the apical foramen area before and after root canal preparation, and after root canal filling. Apical foramen shape and apical transportation, as well as its level of circumferential filling after root canal preparation were accessed using the Image Subtraction System. Scanning electronic microscopy analysis demonstrated that samples of Group 1 showed larger foraminal diameter than samples of Group 2 (p<0.05). Apical foramen transportation was statistically different between Groups 1 and 2 (p=0.0108). Furthermore, the apical foramen sealing also differed statistically between groups 1 and 2 (p=0.0007) and 100% of samples of Group 1 showed apical root canal sealing. Apical root canal sealing was more effective when the root canal was prepared with apical foramen enlargement, even when the apical transportation was detected.


2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Mustaffa M ◽  
Nordin N ◽  
Embong SNH ◽  
Mohd Ibrahim MS

INTRODUCTION: This study compared the obturated surface area, the extrusion of root filling material beyond the apical foramen and the duration of obturation procedure in single-rooted mandibular premolar using monocone obturation technique. MATERIALS AND METHODS: The root canal of twenty single-rooted mandibular premolars were prepared, and then divided into two groups; Group 1 (GuttaFlow Bioseal (GFB) and a gutta-percha (GP) cone) and Group 2 (RoekoSeal Automix root canal sealer and a GP cone). The obturation procedure was timed and the obturation radiograph was taken after the procedure. The roots were sectioned perpendicularly and the surfaces were observed under scanning electron microscope. The images were transferred to the SketchAndCalc Area Calculator software for evaluation of the obturated surface area. RESULTS: The median score of obturated surface area in Group 1 and 2 at the apical was 86.51 and 83.00, at the middle was 90.48 and 87.35 and at the coronal was 93.00 and 83.39, respectively. The extrusion of root filling material between two groups did not show statistically significant difference. The mean duration of obturation in Group 1 and 2 was 149.50 and 137.60, respectively. CONCLUSIONS: The obturated surface area at the apical and middle root regions in Group 1 and 2 was comparable but at the coronal region, Group 1 showed 11.5% better coverage. The extrusion of root filling material in Group 1 and 2 was equivalent. Obturation procedure in Group 1 took 8.6% longer than in the Group 2.


2018 ◽  
Vol 6 (8) ◽  
pp. 1475-1479 ◽  
Author(s):  
Dalia Y. Zaki ◽  
Mohamed H. Zaazou ◽  
Maram E. Khallaf ◽  
Tamer M. Hamdy

BACKGROUND: The composition of the root canal filling materials together with the apical limit of the root canal obturation affect the complete periapical healing after root canal therapy.AIM: This study was performed to evaluate and compare the periapical healing in response to calcium-silicate (iRoot SP) and calcium-hydroxide (Apexit) based-sealers.MATERIAL AND METHODS: Seventy-two upper premolars root canals of six dogs were used. The teeth were randomly assigned to four groups: Group one: roots were obturated using gutta-percha and Apexit-sealer; Group two: roots were obturated using gutta-percha&iRoot SP-sealer; Group three: the teeth were left open without obturation; Group four: where healthy teeth were used as a negative control. Teeth were evaluated after one, two and three months. The newly formed mineralised apical tissue and the periapical inflammatory infiltrate of the obtained photomicrographs were evaluated, and scorings were statistically-analysed.RESULTS: The mean percentage of the periapical inflammatory infiltrates and mineralisation scoring after one, two and three months evaluation period were not significantly different among the four groups (P > 0.05).CONCLUSIONS: Regardless of the sealer used, iRoot SP and Apexit promote healing of periapical tissues. IRoot SP sealer showed early insignificant more partial and almost full healing after two and three months.


2018 ◽  
Vol 28 (2) ◽  
pp. 46-51
Author(s):  
Martin Hashemi ◽  
Miglė Mackevičiūtė

Today it is more and more preferred to preserve teeth with promising prognosis rather than to extract them. The efficiency and the precision of retreatment files to remove filling material are important factors of successful treatment. The aim of this study was to evaluate the efficiency of MaxWire® alloy file in removing root canal filling material after retreatment with endodontic retreatment instruments. Distal roots of twenty human mandibular molars with single and straight canals were selected for this study. All canals were instrumented up to #40/.04 with Bio-race system and apical preparation was finished with Kfile #50/.02. Obturation was performed using lateral condensation technique with ADSEAL sealer. Radiographs were performed in buccolingual and mesiodistal directions with periapical X-ray after obuturation. Teeth were randomly divided into two groups with ten teeth in each. Group 1: removal of root canal filling material was performed using D-race system. Group 2: removal of root canal filling material was performed using D-race system following XP endo Finisher R instrument. Radiographs were performed after retreatment. Residual material was calculated in percentage left in canals. Statistical significance between the two groups were analyzed with t-test. Remnants of root canal filling material was observed in both groups: 38.90% in Group 1 and 13.71% in Group 2, respectively. There was a significant difference between the groups in term of the total root canal filling material (p&amp;lt;0.05). MaxWire® alloy file significantly increased the amount of removed root canal filling material after retreatment with endodontic retreatment instruments.


2018 ◽  
Vol 6 (11) ◽  
pp. 2193-2197
Author(s):  
Roohollah Sharifi ◽  
Amin Torabi ◽  
Reza Hatam ◽  
Nafiseh Nikkerdar ◽  
Hamid Reza Mozaffari ◽  
...  

AIM: This study was conducted to investigate the effect of applying Gates-Glidden (GG) drill by pre-clinical dental students on root canal treatment quality. METHOD: A total of 56 first molars consisting of 168 canals were selected in this study. For this purpose, 56 students who had been formerly trained by two methods of root canal preparation were randomly divided into two groups (n = 28). Group 1: the step-down method by GG and Group 2: step-back technique without GG. The prepared teeth were filled with gutta-percha/ZOE sealer using lateral condensation. Periapical radiographs were taken before and the following treatment to survey occurrence of preparation errors and CBCT images to determine residual dentine at furcation region. RESULTS: The findings showed that among 10 error types in specimens prepared by students, the occurrence of underfilling, overfilling, inappropriate, ledge formation, and single cone was more common without GG. There were no significant differences in residual dentine amount at furcation region between preparation with and without using GG (P > 0.05). CONCLUSION: Using GG for root canal preparation by dental students resulted in low errors and not an increased dentine removal risk.


2016 ◽  
Vol 62 (4) ◽  
pp. 204-208
Author(s):  
Sînziana Adina Scărlătescu ◽  
◽  
Irina Maria Gheorghiu ◽  
Stana Păunica ◽  
Paula Perlea ◽  
...  

The purposes of retreatment are to eliminate the cause for failure of initial endodontic treatment and to preserve the tooth as well. The aim of this study is a comparative evaluation of efficacy of Protaper rotaty retreatment instruments for gutta-percha removal with or without solvents specific both to the sealer and gutta-percha. Materials and methods: Forty single-rooted extracted teeth were randomly divided into 4 groups according to different methods of retreatment. The teeth were prepared with Protaper Universal system (Dentsply-Maillefer, Ballaigues, Switzerland) and filled using lateral condensation of gutta-percha and AH Plus (Dentsply DeTrey, Konstanz, Germany). All root canal fillings were removed using Protaper Universal Retreatment system (Dentsply-Maillefer, Ballaigues, Switzerland). During the retreatment procedure, in group 1 Resosolv (Pierre Rolland, Merignac, France) was used; in group 2 eucalyptol was used, in group 3 Endosolv E (Septodont, Paris, France) was used and in group 4 no solvent was used. The times required for retreatments and radiographycal evaluation after retreatment were recorded. Results: All root canals showed filling material remnants and group 1 with specific solvent for AH Plus recorded the shortest time. Conclusions: Although it is not possible to eliminate the entire filling material on the canal walls, the duration of retreatment was reduced by the use of a solvent specific to the sealer compared with a gutta-percha solvent or no solvent.


2019 ◽  
Vol 25 (4) ◽  
pp. 161-165
Author(s):  
Scarlatescu Sanziana ◽  
Gheorghiu Irina-Maria ◽  
Mitran Loredana ◽  
Mitran Mihai ◽  
Perlea Paula

Abstract Calcium hydroxide is the most used intracanalar medicament between apointments. It shoud be properly removed before root canal filling because its remnants could compromise the sealing of root obturation. The aim of this study was to compare the effectiveness of two type of irrigation solutions, EDTA and SmearClear in the removal of calcium hydroxide from root canals. Materials and method: twenty human single rooted teeth were prepared using ProTaper Universal instrumentation till F2, .06 taper. The canals were filled with Ca(OH)2 and after 7 days the Ca(OH)2 was removed using 3 rinses of 5 ml 15% EDTA and 5 ml 5.25% NaOCl alternately (group 1; n=10) or 3 rinses of 5 ml Smear Clear (SybronEndo) and 5 ml 5.25% NaOCl alternately (group 2; n=10). Results: On the final radiographs the best result was found in group 2 where the cleanliness of the total surface of the root canal wall when SmearClear was used obtained better results comparative to NaOCl – 15% EDTA (91.34% to 71.69%). Conclusions: under the limitation of this study, SmearClear was superior in removing Ca(OH)2 material compared to NaOCl and 15% EDTA irrigation solution.


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