scholarly journals Non-Ionizing Millimeter Waves Non-Thermal Radiation of Saccharomyces cerevisiae—Insights and Interactions

2021 ◽  
Vol 11 (14) ◽  
pp. 6635
Author(s):  
Ayan Barbora ◽  
Shailendra Rajput ◽  
Konstantin Komoshvili ◽  
Jacob Levitan ◽  
Asher Yahalom ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Division ◽  
Millimeter Waves ◽  
Model Organism ◽  
Horn Antenna ◽  
Colony Growth ◽  
Genetic Alterations ◽  
Power Delivery ◽  
Repair Gene ◽  
Safety Standards

Non-ionizing millimeter-waves (MMW) interact with cells in a variety of ways. Here the inhibited cell division effect was investigated using 85–105 GHz MMW irradiation within the International Commission on Non-Ionizing Radiation Protection (ICNIRP) non-thermal 20 mW/cm2 safety standards. Irradiation using a power density of about 1.0 mW/cm2 SAR over 5–6 h on 50 cells/μL samples of Saccharomyces cerevisiae model organism resulted in 62% growth rate reduction compared to the control (sham). The effect was specific for 85–105 GHz range and was energy- and cell density-dependent. Irradiation of wild type and Δrad52 (DNA damage repair gene) deleted cells presented no differences of colony growth profiles indicating non-thermal MMW treatment does not cause permanent genetic alterations. Dose versus response relations studied using a standard horn antenna (~1.0 mW/cm2) and compared to that of a compact waveguide (17.17 mW/cm2) for increased power delivery resulted in complete termination of cell division via non-thermal processes supported by temperature rise measurements. We have shown that non-thermal MMW radiation has potential for future use in treatment of yeast related diseases and other targeted biomedical outcomes.

scholarly journals Non-Ionizing Millimeter Waves Non-thermal Radiation of Saccharomyces Cerevisiae – Insights and Interactions.

2021 ◽  
Author(s):  
Ayan Barbora ◽  
Sailendra Rajput ◽  
Konstantin Komoshvili ◽  
Jacob Levitan ◽  
Asher Yahalom ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Division ◽  
Millimeter Waves ◽  
Model Organism ◽  
Horn Antenna ◽  
Colony Growth ◽  
Genetic Alterations ◽  
Power Delivery ◽  
Repair Gene ◽  
Safety Standards

Nonionizing millimeter-waves (MMW) interact with cells in a variety of ways. Here the inhibited cell division effect was investigated using 85-105 GHz MMW irradiation within the ICNIRP (International Commission on Non-Ionizing Radiation Protection) non-thermal 20 mW/cm2 safety standards. Irradiation using a power density of about 1.0 mW/cm2 , SAR over 5-6 hours on 50 cells/μl samples of Saccharomyces cerevisiae model organism resulted in 62% growth rate reduction compared to the control (sham). The effect was specific for 85-105 GHz range, and was energy and cell density dependent. Irradiation of wild type and Δrad52 (DNA damage repair gene) deleted cells presented no differences of colony growth profiles indicating non-thermal MMW treatment does not cause permanent genetic alterations. Dose versus response relations studied using a standard horn antenna (~1.0 mW/cm2) and compared to that of a compact waveguide (17.17 mW/cm2) for increased power delivery resulted in complete termination of cell division via non-thermal processes supported by temperature rise measurements. We have shown that non-thermal MMW radiation has potential for future use in treatment of yeast related diseases and other targeted biomedical outcomes.

scholarly journals Non-Thermal Millimeter Waves Non-Ionizing Radiation of Saccharomyces Cerevisiae – Insights and Interactions

2021 ◽  
Author(s):  
Ayan Barbara ◽  
Shailendra Rajput ◽  
Konstantin Komoshvili ◽  
Jacob Levitan ◽  
Asher Yahalom ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Division ◽  
Ionizing Radiation ◽  
Millimeter Waves ◽  
Model Organism ◽  
Horn Antenna ◽  
Colony Growth ◽  
Genetic Alterations ◽  
Repair Gene ◽  
Safety Standards

Nonionizing millimeter-waves (MMW) interact with cells in a variety of ways. Here the inhibited cell division effect was investigated using 85-105 GHz MMW irradiation within the ICNIRP (International Commission on Non-Ionizing Radiation Protection) non-thermal 20 mW/cm2 safety standards. We irradiated using radiation with a power density of about 1.0 mW/cm2 over 5-6 hours on 50 cells/μl samples of Saccharomyces cerevisiae model organism. This resulted in 62% growth rate reduction compared to the control (sham). The effect was specific for 85-105 GHz range, and was energy and cell density dependent. Irradiation of wild type and Δrad52 (DNA damage repair gene) deleted cells presented no differences of colony growth profiles indicating non-thermal MMW treatment does not cause permanent genetic alterations. Dose versus response relations studied using a standard horn antenna (~1.0 mW/cm2) and compared to that of a compact waveguide (17.17 mW/cm2) for increased power delivery resulted in complete termination of cell division via non-thermal processes supported by temperature rise measurements. Combinations of MMW mediated Structure Resonant Energy Transfer (SRET), membrane modulations eliciting signaling effects, and energetic resonance with biomolecules are conjectured to be responsible for the observations reported. Our results suggest innovative applications of nonionizing radiation procedures for yeast related diseases and other targeted biomedical outcomes.

scholarly journals Non-thermal Millimeter Waves Non-Ionizing Radiation of Saccharomyces Cerevisiae – Insights and Interactions.

2020 ◽  
Author(s):  
Ayan Barbara ◽  
Shailendra Rajput ◽  
Konstantin Komoshvili ◽  
Jacob Levitan ◽  
Asher Yahalom ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Division ◽  
Ionizing Radiation ◽  
Millimeter Waves ◽  
Model Organism ◽  
Horn Antenna ◽  
Colony Growth ◽  
Genetic Alterations ◽  
Repair Gene ◽  
Safety Standards

Nonionizing millimeter-waves (MMW) interact with cells in a variety of ways. Here the inhibited cell division effect was investigated using 85-105 GHz MMW irradiation within the ICNIRP (International Commission on Non-Ionizing Radiation Protection) non-thermal 20 mW/cm2 safety standards. We irradiated using radiation with a power density of about 1.0 mW/cm2 over 5-6 hours on 50 cells/μl samples of Saccharomyces cerevisiae model organism. This resulted in 62% growth rate reduction compared to the control (sham). The effect was specific for 85-105 GHz range, and was energy and cell density dependent. Irradiation of wild type and Δrad52 (DNA damage repair gene) deleted cells presented no differences of colony growth profiles indicating non-thermal MMW treatment does not cause permanent genetic alterations. Dose versus response relations studied using a standard horn antenna (~1.0 mW/cm2) and compared to that of a compact waveguide (17.17 mW/cm2) for increased power delivery resulted in complete termination of cell division via non-thermal processes supported by temperature rise measurements. Combinations of MMW mediated Structure Resonant Energy Transfer (SRET), membrane modulations eliciting signaling effects, and energetic resonance with biomolecules are conjectured to be responsible for the observations reported. Our results suggest innovative applications of nonionizing radiation procedures for yeast related diseases and other targeted biomedical outcomes.

scholarly journals Mechanism of the Non-thermal Effect of Millimeter Wave Irradiation on Cell Growth

2020 ◽  
Author(s):  
Ayan Barbara ◽  
Shailendra Rajput ◽  
Konstantin Komoshvili ◽  
Jacob Levitan ◽  
Asher Yahalom ◽  
...  
Keyword(s):  
Cell Division ◽  
Model Organism ◽  
Horn Antenna ◽  
Colony Growth ◽  
Genomic Alteration ◽  
Plain Water ◽  
Non Invasive ◽  
Damage Repair ◽  
Microbial Infections ◽  
And Control

Nonionizing millimeter-waves (MMW) are reported to inhibit cell division of lung cancer cells. In this article, we present a mechanism for the effect of inhibited cell division upon 85-105 GHz MMW irradiation. Strains of cell division model organism Saccharomyces cerevisiae cultured under physiological conditions were analyzed for the effects of MMW exposure. Irradiated cells showed a reduced growth rate than that of control (sham) cells. DNA damage repair mutant (rad52) strain cells were also subjected to MMW exposure to identify the involvement of genomic alteration(s) in this process. Irradiated wild type and rad52 mutant strains showed similar colony growth profiles indicating MMW treatment does not alter genomic DNA. Further, MMW interaction with cytological water was explored as a possible mechanism of action. Cells absorbed more power as compared to plain water. MMW irradiation highly absorbed by the cytological water content likely affects proteomic changes, accounting for the observed effects of inhibited cell division. Irradiations using a standard horn antenna were compared to that of a compact waveguide for increased power which led to complete termination of cell division. Our results provide indications of the development of non-invasive nonionizing irradiation procedures to treat tumor metastasis and control microbial infections.

scholarly journals Cardiolipin-Containing Lipid Membranes Attract the Bacterial Cell Division Protein DivIVA

2021 ◽  
Vol 22 (15) ◽  
pp. 8350
Author(s):  
Naďa Labajová ◽  
Natalia Baranova ◽  
Miroslav Jurásek ◽  
Robert Vácha ◽  
Martin Loose ◽  
...  
Keyword(s):  
Cell Division ◽  
Lipid Bilayers ◽  
Bacterial Cell ◽  
Lipid Membranes ◽  
Model Organism ◽  
Active Role ◽  
Membrane Curvature ◽  
Bacterial Cell Division ◽  
Division Site ◽  
Clostridioides Difficile

DivIVA is a protein initially identified as a spatial regulator of cell division in the model organism Bacillus subtilis, but its homologues are present in many other Gram-positive bacteria, including Clostridia species. Besides its role as topological regulator of the Min system during bacterial cell division, DivIVA is involved in chromosome segregation during sporulation, genetic competence, and cell wall synthesis. DivIVA localizes to regions of high membrane curvature, such as the cell poles and cell division site, where it recruits distinct binding partners. Previously, it was suggested that negative curvature sensing is the main mechanism by which DivIVA binds to these specific regions. Here, we show that Clostridioides difficile DivIVA binds preferably to membranes containing negatively charged phospholipids, especially cardiolipin. Strikingly, we observed that upon binding, DivIVA modifies the lipid distribution and induces changes to lipid bilayers containing cardiolipin. Our observations indicate that DivIVA might play a more complex and so far unknown active role during the formation of the cell division septal membrane.

scholarly journals In Vivo Production of RNA Aptamers and Nanoparticles: Problems and Prospects

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1422
Author(s):  
Ousama Al Shanaa ◽  
Andrey Rumyantsev ◽  
Elena Sambuk ◽  
Marina Padkina
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Large Scale ◽  
Potential Model ◽  
Model Organism ◽  
Rna Aptamers ◽  
Early Stages ◽  
Near Future

RNA aptamers are becoming increasingly attractive due to their superior properties. This review discusses the early stages of aptamer research, the main developments in this area, and the latest technologies being developed. The review also highlights the advantages of RNA aptamers in comparison to antibodies, considering the great potential of RNA aptamers and their applications in the near future. In addition, it is shown how RNA aptamers can form endless 3-D structures, giving rise to various structural and functional possibilities. Special attention is paid to the Mango, Spinach and Broccoli fluorescent RNA aptamers, and the advantages of split RNA aptamers are discussed. The review focuses on the importance of creating a platform for the synthesis of RNA nanoparticles in vivo and examines yeast, namely Saccharomyces cerevisiae, as a potential model organism for the production of RNA nanoparticles on a large scale.

scholarly journals A Recombination Repair Gene of Schizosaccharomyces pombe, rhp57, Is a Functional Homolog of the Saccharomyces cerevisiae RAD57 Gene and Is Phylogenetically Related to the Human XRCC3 Gene

Genetics ◽  
2000 ◽  
Vol 154 (4) ◽  
pp. 1451-1461 ◽  
Author(s):  
Yasuhiro Tsutsui ◽  
Takashi Morishita ◽  
Hiroshi Iwasaki ◽  
Hiroyuki Toh ◽  
Hideo Shinagawa
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Schizosaccharomyces Pombe ◽  
Genomic Library ◽  
Synthetic Lethality ◽  
Multicopy Plasmid ◽  
Repair Gene ◽  
Recombination Repair ◽  
Xrcc3 Gene ◽  
Γ Rays ◽  
Lower Temperature

Abstract To identify Schizosaccharomyces pombe genes involved in recombination repair, we identified seven mutants that were hypersensitive to both methyl methanesulfonate (MMS) and γ-rays and that contained mutations that caused synthetic lethality when combined with a rad2 mutation. One of the mutants was used to clone the corresponding gene from a genomic library by complementation of the MMS-sensitive phenotype. The gene obtained encodes a protein of 354 amino acids whose sequence is 32% identical to that of the Rad57 protein of Saccharomyces cerevisiae. An rhp57 (RAD57 homolog of S. pombe) deletion strain was more sensitive to MMS, UV, and γ-rays than the wild-type strain and showed a reduction in the frequency of mitotic homologous recombination. The MMS sensitivity was more severe at lower temperature and was suppressed by the presence of a multicopy plasmid bearing the rhp51 gene. An rhp51 rhp57 double mutant was as sensitive to UV and γ-rays as an rhp51 single mutant, indicating that rhp51 function is epistatic to that of rhp57. These characteristics of the rhp57 mutants are very similar to those of S. cerevisiae rad57 mutants. Phylogenetic analysis suggests that Rhp57 and Rad57 are evolutionarily closest to human Xrcc3 of the RecA/Rad51 family of proteins.

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